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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 10 % human AB serum (Sigma-Aldrich), and 10 ng/ml recombinant human macrophage colony stimulating factor (Peprotech ...
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bioRxiv - Cancer Biology 2024Quote: ... raised against human ST3GAL1 (Sigma HPA040466) and ST3GAL2 (Abcam ab96028) ...
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bioRxiv - Bioengineering 2024Quote: IgG from human serum (Sigma-Aldrich) was incubated at 62°C for 30 min to induce aggregation ...
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bioRxiv - Bioengineering 2024Quote: ... or human complement C1q (Sigma-Aldrich) were immobilized on a CM5 or C1 sensor chip (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... 4 mg/mL human fibrinogen (Sigma), 10% Matrigel (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% human serum albumin (Sigma-Aldrich), 0.0002% heparin (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... and LbaCas12a protein expression for protein purification utilized pET-21a protein expression plasmids (Novagen) (Figure 1).1,25 AspCas12a ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were seeded in 6-well plates (3.0 × 105 cells/well) coated with poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... Then, wildtype HEK293 cells (ATCC, CRL-1573) were infected in regular growth medium with 5 µg/mL polybrene (Sigma-Aldrich, TR-1003-G) and the lentivirus at a multiplicity of infection of 5 viral particles per cell ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of Cypridina noctiluca luciferase and Omicron RBD or its mutants were co-transfected (9:1) into HEK293 cells using X-tremeGENE 9 (Millipore-Sigma XTG9-RO). After 16 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2×YT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were then added directly to 96-well His-Select Ni-NTA resin filter plates (Sigma Aldrich) and processed off-robot by centrifugation ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatants were independently applied to a column of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: ... followed by cleavage of the His-tag with 3C protease and dephosphorylation with bovine alkaline phosphatase (Sigma). Further purification was performed by ion exchange chromatography (Source Q for WT and the S897E ...
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bioRxiv - Biophysics 2020Quote: ... and passed through pre-equilibrated HIS-Select Ni-nitrilotriacetic acid resin (Ni-NTA) (Sigma-Aldrich Co., USA) at 4 °C for binding of 6x-His tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...
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bioRxiv - Cell Biology 2019Quote: ... The His-tag was removed by cleavage with 16 U of thrombin (Ref 27-0846-01, Sigma) added directly onto the beads for 2h at 25°C ...
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bioRxiv - Biochemistry 2021Quote: ... pneumoniae LicB with an N-terminal 10×His affinity tag in a modified pET-19b vector (Novagen) was overexpressed in E ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... After several rounds of washing with His-AC washing buffer (supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: Human ovarian cancer A2780 and Adriamycin resistant human ovarian cancer A2780-R cells were procured from Sigma Inc ...
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bioRxiv - Genetics 2022Quote: ... Human Erythroleukemia (HEL) and human TF-1 cells were cultured in RPMI-1640 medium (Sigma Aldrich #R8758) supplemented with 10% Fetal Bovine Serum (Sigma Aldrich #F0926 ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte- human fibrinogen mixture (Sigma-Aldrich). Then ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte-human fibrinogen mixture (Sigma-Aldrich) and 2µL of the mixture is carefully pipetted into the microwell ...
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bioRxiv - Evolutionary Biology 2020Quote: ... three replicate groups of 22 ants were supplied with 300 μl of a 20% 13C glucose solution (w/v, Sigma Aldrich) and the remaining three groups were supplied with 300 μl of a 20% 12C glucose solution (w/v ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we treated females in the Pregnancy GCs (n = 44 total litters from 43 unique females) and Lactation GCs (n = 18 litters from 17 unique females) treatment groups with exogenous cortisol (hydrocortisone, Sigma H4001) dissolved in peanut butter and wheat germ mixture (8 g of peanut butter ...
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bioRxiv - Bioengineering 2021Quote: ... we labeled a single lysine group on a portion of our ELPs with a cyanine-5 (Cy5) NHS molecule (excitation: 651, emission: 670; Sigma 679011) via standard NHS-ester chemistry ...
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bioRxiv - Microbiology 2020Quote: Infected cells grown on coverslips were fixed with 4% paraformaldehyde and permeabilized/blocked with a Triton-X100 (0.2%)/BSA (3%) solution and then incubated with mouse anti-Flavivirus Group Antigen 4G2 (Millipore, Burlington, MA), mouse anti-alphavirus capsid (kindly provided by Dr ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells from iCD4-PRMT5fl/fl and iCD4-PRMT5Δ/Δ mice (n = 3 pooled mice/sample and n = 3 samples/group) were lysed in our in-house lysis buffer (50 mM triethylammonium bicarbonate, Millipore Sigma, catalog no ...
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bioRxiv - Biochemistry 2022Quote: ... the digested tryptic peptides from the pull-down samples and from the control group were desalted using C18 ziptips (EMD Millipore). Samples were further reconstituted in liquid chromatography-mass spectrometry (LC-MS ...
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bioRxiv - Immunology 2019Quote: C57BL/6 mice (female, 9-10 weeks old) were nebulised with LPS (3 mg per group of 8 mice) (Pseudomonas aeruginosa, Sigma-Aldrich) and immediately injected intraperitoneally (i.p. ...
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bioRxiv - Immunology 2021Quote: ... 721.221 was purchased from the International Histocompatibility Working Group and grown in RPMI-1640 supplemented with 15% heat-inactivated fetal calf serum (FCS) (Sigma-Aldrich), sodium pyruvate (Thermo Fisher) ...
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bioRxiv - Microbiology 2019Quote: Frozen larvae were homogenised in group of 5 larvae in extraction buffer (0,01 % IGEPAL® CA-630 (Sigma-Aldrich, Saint Louis), 1 mM PMSF ...
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Cold exposure drives weight gain and adiposity following chronic suppression of brown adipose tissuebioRxiv - Physiology 2021Quote: ... clean up and trypsinisation was performed on 50-100 mg of frozen tissue (n=4/group) was homogenised in 500 μL CellLytic MT cell lysis buffer (Sigma, C3228) prior to removal of lipid and other contaminants using the ReadyPrep 2D cleanup Kit (Biorad ...
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bioRxiv - Cancer Biology 2020Quote: ... mice bearing tumors with similar sizes (determined by live animal imaging of bioluminescence) were randomly divided into either a “vehicle control” group (0.5 methylcellulose, Sigma-Aldrich #M0430) or a PRMT5 inhibitor “GSK595 treatment” group (100 mg/kg) ...
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bioRxiv - Cell Biology 2021Quote: Mice in shame and MI groups were anesthetized by intraperitoneal injection of 15 mg/mL tribromoethanol (0.01 mL/g) (Sigma-Aldrich, T48402) on day 0 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a control where females were fed a mixture of honey and water (1:2 w/w) or a treatment group fed a mixture of honey and deuterated water (Sigma Aldrich)(1:2 w/w ...
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bioRxiv - Molecular Biology 2021Quote: ... n = 3 for each group) were intraperitoneally injected with lipopolysaccharide (LPS; Escherichia coli 0111:B4, Sigma-Aldrich, St Louis, MO, USA), polyinosinic:polycytidylic acid [poly(I:C) ...
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bioRxiv - Cell Biology 2021Quote: ... groups conferring overall positive charge (TdB, DD4) were dissolved in injection buffer (50 mM K-HEPES, pH 7.4 (Sigma-Aldrich, H3375), 25% glycerol (Applichem ...
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bioRxiv - Biochemistry 2022Quote: ... Protein pellet of each SA+ and SA-group was obtained by removing the aqueous and organic layers consecutively following the addition of 4 volumes of methanol (Sigma, #34860), 1 volume of chloroform (ACROS Organics ...
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bioRxiv - Cell Biology 2022Quote: ... R4-R5 section) from both control and PFA-treated group were dissected in Shields and Sang M3 Insect Media (Sigma, S8398) with or without 1 mM PFA added ...
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bioRxiv - Neuroscience 2023Quote: ... in a paired fashion, randomly assigned to either control or bumetanide group (20 μM, Sigma, B3023, stock solution prepared in DMSO (Sigma D8418) prior to dilution in HEPES-aCSF to a final DMSO concentration of 0.1% as also employed as vehicle) ...
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bioRxiv - Microbiology 2023Quote: Newborn mice were randomized to treatment groups with PBS control or incremental intranasal doses of Escherichia coli 055: B5 LPS (Millipore Sigma). Pups were treated on postnatal day 3 (PN3 ...