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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for 1 tert Butoxy 4 methyl 2 nitrobenzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Membranes of primary motor neuron samples were incubated overnight at 4°C with primary antibodies for ERK1/2 (1:10000, Sigma Aldrich) and ATPB (1:1000 ...
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bioRxiv - Pathology 2022Quote: ... 1 part NTA), incubated (30min., RT) and centrifuged (3000g, 1h, 4°C) in an Ultra-2 Centrifugal Filter Unite (Millipore, #UFC203024). Filtered samples (100µL ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were diluted in PBS and sections were incubated for 1 hour at room temperature with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for nuclei visualization ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of these extracts were centrifuged at 14,000 xg for 1 min at 4°C with 2.5 μl 2% (v/v) Tween 20 (Sigma-Aldrich, Darmstadt, Germany) to promote the aggregation into a pellet ...
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bioRxiv - Genetics 2022Quote: ... Bb was cultured at 37ºC in Barbour-Stonner-Kelly with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer media (BSK-H) complete with 6% rabbit serum (Millipore Sigma) and 1% Amphotericin B (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
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bioRxiv - Neuroscience 2020Quote: ... The cells were centrifuged for 5 min at 700g and treated for 10 min with DAPI (4’,6-diamidino-2-phenylindole) (1:4000, Sigma, 62248) to label dead cells ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 4% formaldehyde solution diluted in PBS during 2 hours and stained with 1% Naphtol blue black (Sigma, USA) solution for 1 hour for plaque visualization ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then rinsed 4 times with the same medium free of GFP-nanobodies and fixed at room temperature for 2 hours with PBS supplemented with 4% paraformaldehyde (PFA) and 1% BSA (A7638 Sigma-Aldrich). Open-books were observed with a STED microscope (TCS SP8 ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 20 minutes at room temperature with DAPI (4',6-diamidino-2-phenylindole; 1:150000, Sigma-Aldrich), mounted on glass slides with Fluoromount-G (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then washed three times in PBS and incubated with 4’-6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma-Aldrich) at room temperature for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The transfection plasmids were mixed in a 4:2:1 ratio in Opti-MEM medium utilizing the polycationic agent polyethylenimine (PEI; Sigma-Aldrich). Following transfection ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 1 min with 4’,6-diamidino-2-phenylindole (DAPI; 0.5 μg/ml, Sigma-Aldrich, cat# D8417) diluted in PBS to label cell nuclei ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... DOI (1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride-hydrochloride) and serotonin were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). LP44 (4-[2-(methylthio)phenyl]-N-(1,2,3,4-tetrahydro-1-naphthalenyl)-1-piperazinehexanamide hydrochloride ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed in PBS followed by a nuclear staining with DAPI (4’,6-diamidino-2-fenylindool, 1:1000 in PBS, 32670, Sigma-Aldrich). All sections were mounted with Mowiol and a glass cover slide ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μL protoplast was mixed with 20 μL DNA (1 – 2 μg total) and 50 μL ice-cold solution 4 (25 g of PEG 6000 or 8000 (Sigma, P2139), 1.47 g of CaCl2.2H2O ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed twice at RT with 0.1% Triton 100X in PBS and then counterstaining with 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) (1:5000, Sigma Millipore, #MBD0015) in PBS for five minutes ...
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bioRxiv - Microbiology 2024Quote: ... 2 ug mL-1 N-(6-(isopropylsulfonyl)quinolin-4-yl)benzo[d]thiazol-5-amine hydrochloride (GSK’872) (Sigma-Aldrich # 5303890001) and 2 ug mL-1 Necrosulfanamide (EMD Millipore #480073 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin, F1141, Sigma-Aldrich) tissue cultureware.
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bioRxiv - Cell Biology 2024Quote: ... gels were incubated in primary antibodies (Supplementary Table 4) diluted to 1:500 in antibody dilution buffer (2% bovine serum albumin (BSA; Sigma, A9647) in 1X PBS ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (B-5-1-2) (1:10000; Sigma-Aldrich T5168, B-5-1-2), mouse anti-GAPDH (1:25000 ...
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bioRxiv - Immunology 2020Quote: ... containing 1% 2-mercaptoethanol (Sigma) and stored at -80°C until the RNA extraction ...
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bioRxiv - Bioengineering 2021Quote: ... with 1% 2-Mercaptoethanol (Sigma). Total RNA was extracted using RNeasy Micro Kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... + 1% 2-Mercaptoethanol (Sigma Aldrich) at -80 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 mM 2-mercaptoethanol (Sigma), and 12.5 µg/mL of basic fibroblast growth factor (bFGF) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 mM 2-mercaptoethanol (Sigma), and 1000 units/mL leukemia inhibitory factor ...
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bioRxiv - Neuroscience 2021Quote: ... 2 U.μl-1 Benzonase (Novagen) and 4 mM MgCl2 ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 2-hexanone (Sigma 103004), 1% 2-phenylethanol (Sigma 77861) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 2-phenylethanol (Sigma 77861), 1% 3-methyl-1-butanethiol (Sigma W385808) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 2-heptanone (Sigma 53768315), 1% linalool (Sigma L2602) ...
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bioRxiv - Neuroscience 2023Quote: ... containing 1% 2-mercaptoethanol (Sigma) to be able to use for RNA extraction.
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bioRxiv - Developmental Biology 2023Quote: ... 1-phenyl-2-thiourea (Sigma) was added at 30 mg/l to prevent embryonic pigmentation.
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bioRxiv - Genomics 2022Quote: ... with 1% 2-Mercaptoethanol (Sigma), vortexed briefly ...
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bioRxiv - Genomics 2023Quote: ... followed by the addition of 1 uM 4-Hydroxytamoxifen (4-OHT; Sigma, SML1666), when required ...
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bioRxiv - Physiology 2020Quote: ... inhibitor 2-bromohexadecanoic acid (2-bromo-palmitate; 2-Br) [IC50 of ∼4 µM] (Varner et al., 2003) were purchased from Sigma-Aldrich (St. Louis, MO). Bradford protein assay kit as well as anti-mouse and anti-rabbit secondary antibodies were obtained from Bio-Rad (Hercules ...
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bioRxiv - Immunology 2022Quote: ... 2-deoxy-D-glucose (2-DG, 1 mmol/l, Sigma) or diethyl succinate (1 mmol/l ...
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bioRxiv - Cell Biology 2023Quote: ... and 1% photo initiator (2-hydroxy-2-methylpropiophenone; Sigma-Aldrich), was introduced between the PDMS stamp and the glass coverslip using capillary action ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were incubated with 2 μg/mL 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, Missouri, USA) for 10 minutes as the final step in all flow cytometry assays to distinguish membrane integrity and disruption ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: Cells were seeded on coated glass cover (Assistent) slips 2 days before fixation with 4 % paraformaldehyde (Sigma, 30525-89-4) for 15 minutes ...
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bioRxiv - Immunology 2021Quote: PBMCs and tissue MNC suspensions were treated with 5 μM Chk2 inhibitor II or 2-[4-(4-chlorophenoxy)phenyl]-1H-benzimidazole-5-carboxamide (Sigma) prepared in final volume of 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... After addition of 250 µL 4× lysis buffer (4% SDS, 40 mM tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich, C4706), 160 mM chloroacetamide (CAM ...
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bioRxiv - Cell Biology 2024Quote: ... and then seeded at 2000 cells per well in enriched pseudoislet media supplemented with 2 μM 4-hydroxytamoxifen (4-OHT; Sigma) or vehicle control (0.1% ethanol ...
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bioRxiv - Bioengineering 2020Quote: ... SSEA-4 (Millipore, Cat. # MAB4304; 1:100), TRA-1-60 (eBioscience ...
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bioRxiv - Cell Biology 2021Quote: ... syntaxin-4 (rabbit, Millipore AB5330, 1:1000), TMED9 (rabbit ...
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bioRxiv - Microbiology 2020Quote: ... Calpain 4 (1:500, MAB3083, Merck Millipore), Calpastatin (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µM (Z)-4-hydroxytamoxifen (Sigma H7904) was added to the slice culture medium to induce Cre-activation ...