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Citations for Millipore Sigma :
1001 - 1050 of 10000+ citations for 1 tert Butoxy 4 methyl 2 nitrobenzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: Visualization of the accessible pool of cholesterol was performed after 30 min treatment with 10 mM methyl-beta-cyclodextrin (MβCB) in 1% lipoprotein-deficient serum (LPDS, Sigma #S5519) or 1% LPDS control ...
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bioRxiv - Plant Biology 2024Quote: ... The tissue was blotted on tissue paper before weighing to remove excess water and then was transferred into a 1.5mL Eppendorf tube containing 1 mL of di-methyl sulfoxide (DMSO) (#D8418, Sigma Aldrich) and incubated in the dark at 65C with 300 rpm shaking for 45 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and pretreated 1 h at 37°C with either 5 mM Methyl-β-cyclodextrin (MBCD) (Sigma Aldrich, stock in ddH2O) in OptiMEM medium buffered with 25 mM HEPES (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Fully grown oocytes were collected 48 hours after injection and placed in M2 medium containing 200 μM 3-isobutyl-1-methyl-xanthine (IBMX, Sigma) at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... The instrument was calibrated against a poly(ethylene glycol) methyl ether standard (Sigma Aldrich, Average Mn = 2,000 g mol-1) prepared under the same conditions with DCTB matrix.
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bioRxiv - Microbiology 2023Quote: ... 4 µl internal standard (2 g/l L-2-aminoadipic acid, Sigma-Aldrich Chemie GmbH, Buchs, Switzerland), and 3.5 µl diethyl ethoxymethylenemalonate (VWR International AG ...
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bioRxiv - Cancer Biology 2023Quote: ... and fixed in FACS Buffer containing 2 % FBS and 4 % para-formadehyde (Sigma-Aldrich #95-30525-89-4). Zombie Aqua Fixable viability dye (Biolegend # 423101 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1 % tetramethylethylenediamine (TEMED, Roth) and 0.005 % 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (4-hydroxy-TEMPO, Sigma) were added to activate the polymerization reaction ...
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bioRxiv - Bioengineering 2021Quote: ... The secondary antibodies were washed away with PBS (3x 5 min) whereafter 4’,6-diamidino-2-phenylindole (DAPI, 1:500, Sigma) was added for 10 minutes to localize the cell nuclei ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed using 70% Ethanol and re-suspended in PBS containing 1 μg/ml 4’,6-diamino-2-phenylindole (DAPI, Sigma) and analyzed by microscopy ...
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bioRxiv - Developmental Biology 2020Quote: ... The female mice were sacrificed by cervical dislocation and the whole female reproductive tract with the inner contents was collected with the copulation plug at 1 hour or 2 hours after coitus and placed into 4% paraformaldehyde (PFA, Sigma) at 4°C overnight.
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bioRxiv - Molecular Biology 2022Quote: ... in a 10-cm dish transfected one day post seeding with packaging plasmids (1 μg VSV.G, 2 μg psPAX2 and 4 μg of transfer plasmid using PEI (Sigma-Aldrich). Virus containing supernatant was collected 72 hours post transfection ...
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bioRxiv - Neuroscience 2021Quote: ... the NMDA glutamate receptor antagonist [3H]3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP, 10 μM, Sigma-Aldrich) and a selective group III metabotropic glutamate receptor agonist ...
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bioRxiv - Neuroscience 2021Quote: ... nuclear labelling was achieved by incubating the sections for 5 min in DAPI (4′,6-diamidino-2-phenylindole; 1:5000; Sigma). Two final washes in PBS were performed before the sections were mounted on glass slides using Vectashield (Vector Laboratories ...
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bioRxiv - Developmental Biology 2020Quote: ... or 1000 nM of 2-(4-amino-1-isopropyl-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-1H-indol-5-ol (PP242; Sigma, P0037), (2 ...
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bioRxiv - Immunology 2020Quote: ... siLP cells were released by digestion of the tissue with RPMI/4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) supplemented with 60 μg/ml DNaseI (Sigma), and 400 ng/ml of Liberase (Roche Applied Science ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... wells were washed with embryo media before the fish were incubated in media containing 0.01% 2-[4-(dimethylamino) styryl]-1-ethylpyridinium iodide (DASPEI, Sigma-Aldrich) to stain for neuromast mitochondrial activity for 20 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was re-suspended in ice-cold 100 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid) buffer (pH 8.0) containing complete protease inhibitor cocktail (Sigma, USA) and PMSF (phenylmethanesulphonyl fluoride ...
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bioRxiv - Neuroscience 2020Quote: ... 66 These transgenic mice express the GEVI in cortical layer 2/3 pyramidal neurons under the intersectional control of TetO and destabilised Cre-recombinase.70–72 The destabilised Cre-recombinase was stochastically re-stabilised to induce sparse expression of the voltage indicator via two IP injections of a total of 2 × 10−4 mg kg−1 Trimethoprim (TMP, Sigma) over 2 consecutive days as described in Song et al ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue and cells were incubated in 2-(4-amidinophenyl)-1H -indole-6-carboxamidine (DAPI, D-6564, Sigma, 1:1000). Biotinylated-secondary antibodies were from Vector Labs ...
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bioRxiv - Immunology 2022Quote: ... For cell sorting cell suspensions were stained for viability with 1:3000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) immediately before sorting.
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bioRxiv - Developmental Biology 2022Quote: ... sections were washed in PBS and then incubated for 10 minutes in 1:10000 4’,6-diamidino-2-fenilindol (DAPI; Sigma) for nuclei counterstaining ...
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bioRxiv - Microbiology 2021Quote: ... 1X Pen-Strep and 1 μg/mL L-[(toluene-4-sulphonamido)-2-phenyl] ethyl chloromethyl ketone (TPCK)-trypsin (Sigma-Aldrich). The CPE was observed by visual microscopy at 3 and 5 days post-infection (d.p.i. ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% TritonX/PBS) for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I ...
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bioRxiv - Immunology 2022Quote: ... The sections were washed in PBS and incubated 10 min with 4’,6-diamidino-2-phenylindole (DAPI, 1/10,000, Sigma-Aldrich) to visualize the cell nuclei ...
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bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Immunology 2024Quote: ... slides were washed in PBS and incubated in 5 μg mL-1 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... (phenylmethylsulfonyl fluoride, PMSF 1mM; 1-chloro-3-tosylamido-4-phenyl-2-butanone, TPCK, 10 μg/ml; aprotinin, 10 μg/ml Sigma) incubated for 30min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in staining media supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from analyses ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then washed with 0.1% Tris-buffered saline and Tween-20 (TBST) prior to 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (D9542 Sigma) for nuclei visualization ...
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bioRxiv - Cell Biology 2023Quote: ... except the protein sample was concentrated to 4 mg/ml and mixed with 0.005% 3-([3-Cholamidopropyl]dimethylammonio)-2-shydroxy-1-propanesulfonate (CHAPSO; Sigma Aldrich) immediately before vitrification ...
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bioRxiv - Biochemistry 2022Quote: ... and cell pellets were collected and resuspended in the resuspension buffer containing 20 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma) pH 7.4 and 200 mM NaCl (Sigma)] ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed several times in PBS and incubated with 1 µg/mL of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize the nuclei.
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bioRxiv - Cell Biology 2024Quote: ... samples were washed in 1X PBS and incubated with DAPI (4-6-diamidino-2-phenylindole, Sigma-Aldrich, 1 µg/mL) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... in 0.1% BSA/PBS supplemented with 5 μg/ml Hoechst or 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI)) or ExtrAvidinCy3 (Sigma) solution (1:100 in 0.1% BSA/PBS supplemented with 5 μg/ml Hoechst) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in PBS and further incubated for 15 min at room temperature with DAPI (4’, 6-diamidino-2-phenylindole, 1:5000, Sigma). Incubation with the secondary antibody lasted for 3 h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... and 1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol-4’,5’-bisphosphate) (PI(4,5)P2) in chloroform: methanol (Millipore MX0488): water (20:9:1 ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1x 30 min with 5×SSCT containing 4′,6-diamidino-2-phenylindole (DAPI, 1μg/ml, #62248, ThermoFischer) and/or Phalloidin Atto 565 (1:50, #94072, Sigma Aldrich), and 1x 5min in 5xSCCT ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were washed in PBS and further incubated for 15 min at room temperature with DAPI (4’,6-diamidino-2-phenylindole, 1:5000, Sigma). Incubation with the secondary antibody lasted for 3 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μM 4–OHT (Sigma-Aldrich) treatment was used at different time-points (2 ...
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bioRxiv - Biophysics 2022Quote: ... 1 mM 4-nitrobenzyl alcohol (Sigma) and 1 mM Trolox (Sigma)] ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μM 4-hydroxytamoxifen (Sigma) for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 μM 4-Hydroxytamoxifen (Sigma). For P7 CGNPs ...
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bioRxiv - Microbiology 2022Quote: ... 4-Choloro-1-naphthol substrate (Sigma) and H2O2 catalyst (VWR International ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mM 4-MUG (, M9130-Sigma) and incubated at 37 °C for 6 h ...
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bioRxiv - Cell Biology 2024Quote: ... 4-hydroxytamoxifen (OHT, Sigma, 1 µM) was added 24 h post T cell isolation and stimulation ...
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bioRxiv - Bioengineering 2024Quote: ... 1-4,Dithiothreitol (DTT; Sigma-Aldrich) and KCGPQGIWGQCK (MMP-2 degradable peptide ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% of photoinitiator (2-hydroxy-2-methylpropiophenone - Sigma) was immediately introduced by capillary action between the PDMS stamp and the glass coverslip ...