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Citations for Millipore Sigma :
1101 - 1150 of 10000+ citations for Cow Prostaglandin E Synthase 2 PTGES2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 5 μM oligomycin and 100 mM 2-deoxyglucose (2-DG) (Sigma-Aldrich) were injected to each well after 18 ...
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bioRxiv - Immunology 2021Quote: ... 2-Deoxy-D-glucose (2-DG) was obtained from Sigma Aldrich (D8375) and used at 0.32 mM ...
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bioRxiv - Genomics 2020Quote: ... Substrate solution (2 mM ABTS, Sigma A9941; 2 mM hydrogen peroxide, Sigma H1009 ...
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bioRxiv - Cell Biology 2021Quote: ... The last injection was supplemented with 2-deoxyglucose (2-DG, Sigma, D8375) to obtain the ECAR background value due to nonglycolytic acidification ...
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bioRxiv - Immunology 2019Quote: ... 2’-Deoxyadenosine (dAdo) and 2’-Deoxycytidine (dCyt) were purchased from Sigma-Aldrich Japan (Tokyo ...
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bioRxiv - Immunology 2021Quote: ... For the last 2 h 2 µg/ mL Brefeldin A (Sigma-Aldrich) was added ...
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bioRxiv - Immunology 2021Quote: ... 2 ml overlay [MEM containing 2% FBS and 0.4% tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... 55μM 2-mercaptoethanol (2-ME) supplemented with 50 U/ml Benzonase (Sigma). Aliquots derived from the same donors were combined and all samples were enriched for T cells by removing CD14 and CD19 expressing cells using a column-based magnetic depletion approach according to the manufacturer’s recommendations (Miltenyi) ...
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bioRxiv - Immunology 2021Quote: ... cells were treated with 2-deoxyglucose (2-DG) 100 nM (Sigma, #D8375).
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bioRxiv - Immunology 2020Quote: ... 2 ml overlay [MEM containing 2% FBS and 0.4% tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... 3-[2-(2-aminoethylamino)-ethylamino] propyl-trimethoxysilane (DETA) (Sigma-Aldrich, Hamburg, Germany) for microtubule immobilization ...
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bioRxiv - Biophysics 2022Quote: ... and 2 µM 9-Amino-6-Chloro-2-Methoxyacridine (ACMA, Sigma-Aldrich) to establish a K+ gradient ...
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bioRxiv - Biophysics 2024Quote: ... and 3% (wt/vol) hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Sigma, 410896) in ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... 0.15 M 2-amino-2-methyl-1-propanol (AMP) (A65182, Sigma-Aldrich) in UPW ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µM epoxomicin and 50 µM (Z-LL)2-ketone (Merck Millipore) were added from stock solutions in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1µM Oligomycin A and 50mM of 2-deoxyglucose (2-DG) (Sigma, D3179). For mitostress tests ...
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bioRxiv - Developmental Biology 2024Quote: ... dissolved in water and 2-2’ bipyridyl (BP) (Sigma‒Aldrich Cat# D216305) dissolved in DMSO were added to the NGM before pouring to final concentrations of 4 mmol/L ...
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bioRxiv - Bioengineering 2024Quote: ... methyl triflate and 2-ethyl-2-oxazoline were obtained from Sigma Aldrich and dried over CaH ...
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bioRxiv - Biochemistry 2024Quote: ... oligomycin and 2-deoxy-D-glucose (2-DG, Sigma-Aldrich, D8375-1G)) at final well concentrations of 10 mM ...
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bioRxiv - Microbiology 2021Quote: ... were fluorescently labelled by the addition of 2 μl PKH26 dye (1 mM stock concentration) from the MINI26 PKH26 Red Fluorescent Cell Linker Kit (Sigma-Aldrich) and mixed by pipetting ...
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bioRxiv - Immunology 2022Quote: ... CHO CoV-2-Spike cells were used as target cells and were fluorescently labeled with PKH67 Fluorescent Cell Linker Kit (Sigma Aldrich) prior to incubation with mAbs ...
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bioRxiv - Microbiology 2021Quote: ... DNA was then extracted from 2 mL of cell culture using a GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich, USA). DNA samples were subjected to an initial quality check using a Nanodrop spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: Cell viability and cytotoxicity of antibiotics and peptides were determined against human corneal epithelial cells (HCE-2, CRL-11135, ATCC, Manassas, Virginia, USA) using cell-counting-kit-8 (CCK-8) assay (Sigma Aldrich, Merck Life Science UK Limited ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was removed and the cell pellet was resuspended in 500 μL Diluent C containing 2 μL PKH67 dye (PKH67 Green Fluorescent Cell Linker Kit, Sigma-Aldrich), incubated for 5 minutes at room temperature ...
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bioRxiv - Genomics 2019Quote: ... with 2 mL of ice-cold EZ-Prep lysis buffer from the Nuclei EZ-prep Isolation Kit (Sigma, Cat #NUC-101). The tissue was dounced 25 times with Pestle A ...
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bioRxiv - Immunology 2022Quote: ... 100-150 mg of snap-frozen tissue were mechanically homogenized in 750 µL of Lysis Solution/2-mercaptoethanol mixture and total RNA was isolated using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich). Reverse transcription for up to 2 µg of total RNA was performed using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Following primary antibody incubation coverslips were washed 2× in PBS and then the proximity ligation assay was carried out (Duolink In Situ Red Starter kit (Sigma Aldrich) according to the manufacturer’s instructions) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in 96-well plates (2 × 103 cells/well) and analyzed with the MTT Cell Growth Assay Kit II (Sigma-Aldrich) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was extracted from 2 ml of overnight culture from each time point using the GenElute™ Bacterial Genomic DNA kit (Sigma) and 10 ng analysed by qPCR using a Light Cycler 480 real-time PCR system (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... in FibroGRO™ Complete Media Kit supplemented with bFGF (75 ng/mL) and 2% of foetal bovine serum (FBS, Sigma-Aldrich). Only iPSC-cFbs with a passage number ≤ 10 (P10 ...
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bioRxiv - Immunology 2023Quote: Inflammation-associated with neutrophils and monocytes tissue infiltration was indirectly determined by measuring the MPO activity in 2 cm length fragments of distal colon using an MPO colorimetric activity kit assay (Sigma-Aldrich), following the manufactures instructions.
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bioRxiv - Microbiology 2024Quote: Cytotoxicity was studied according to the manufacturer’s instruction either using cytotoxicity LDH assay kit-WST (Dojindo) or by measuring transepithelial electrical resistance (TEER) (Millicell® ERS-2, Millipore) as previously described (25) ...
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bioRxiv - Biochemistry 2019Quote: Insulin and amylin concentration in plasma were determinate by ELISA (Insulin, Cat # EZRMI-13K and Amylin, Cat # EZHA-52K, Merck Millipore) following manufacturer manual ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were lysed and processed for cell death detection ELISA assay by following the manufacturer’s instruction (#11774425001, Millipore Sigma). At the end of the assay ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Insulin concentration of the supernatants was measured with a mouse/rat insulin ELISA (EZRMI-13K, EMD Millipore, Billerica, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plates were then rewashed three times with PBST before the addition of 3,3’,5,5’-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma-Aldrich, UK) and incubated for 12 minutes in the dark at RT ...
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bioRxiv - Immunology 2022Quote: Stable knockdown of host proteins in THP1 cells was carried out by transduction with lentivectors expressing individual shRNAs (pLKO.1, 400 ng p24Gag (as measured by ELISA) per 1 × 106 cells) in the presence of polybrene (Millipore). Cells were washed and cultured for 5-7 days in the presence of puromycin (2 µg/ml ...
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bioRxiv - Immunology 2019Quote: ... The peritoneal lavage fluids were collected and concentrated for ELISA analysis with Amicon Ultra 10 K filter (UFC900308) from Millipore. Peritoneal exudate cells were collected and analyzed by FACS.
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bioRxiv - Microbiology 2020Quote: ... The ELISA plates were then blocked with 300 μL of blocking buffer (1% BSA and 10% bovine calf serum (BCS) (Sigma) in PBS at 37°C for 2 hrs ...
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bioRxiv - Microbiology 2021Quote: ... and stored at −80°C until particle concentration assessment by p24 ELISA (Innogenetics) while the rest of the supernatant was concentrated by filtration on Centricon units (Centricon Plus-70/100K, Millipore) and frozen at −80°C for RNA extraction ...
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Brain cell-specific origin of circulating microRNA biomarkers in experimental temporal lobe epilepsybioRxiv - Neuroscience 2023Quote: ... Lysates were removed and protein antibody-conjugates linked to the bottom of the plate were washed three times with 300 µL of ELISA wash buffer (100mM Tris buffered saline, pH8.2, 2mM MgCl2, 0.5% Tween-20 – all Sigma-Aldrich, Ireland). Protein was eluted by incubating wells in 2 X Laemelli buffer for 10 mins ...
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bioRxiv - Immunology 2022Quote: ... antibody levels were detected using a secondary anti-murine IgG conjugated with alkaline phosphatase and the substrate for alkaline phosphatase yellow (pNPP) liquid substrate system for ELISA (cat. P7998, Sigma). After 30 minutes of incubation ...
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bioRxiv - Synthetic Biology 2021Quote: ... AraC (2 μM, Sigma) was added at 2 days in vitro (DIV 2) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 M acrylamide (Sigma), 90 ppm N,N’-methylenebisacrylamide (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 2-DG (Sigma-Aldrich), CCCP (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM glutamine (Sigma), and 10 mM glucose (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... or Binuclein 2 (Sigma; #B1186 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM Tubacin (Sigma) or 2 mM Niltubacin (negative control for Tubacin ...
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bioRxiv - Evolutionary Biology 2021Quote: ... in 2% BSA (Sigma) for permeabilization ...
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bioRxiv - Genomics 2020Quote: ... 1x 2-Mercaptoethanol (Millipore), 1x NEAA (Stemcell Technologies) ...