Labshake search
Citations for Millipore Sigma :
1051 - 1100 of 10000+ citations for Cow Prostaglandin E Synthase 2 PTGES2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2019Quote: Embryos designated for hematoxylin and eosin (H&E) staining were dissected and fixed overnight in 10% neutral buffered formalin solution (Sigma-Aldrich) at room temperature (RT ...
-
bioRxiv - Microbiology 2019Quote: ... Macrophages were counted by haemocytometer and diluted to a concentration of 1×105 cells per ml in DMEM supplemented with 1 μg/ml lipopolysaccharide (LPS from E. coli, Sigma L2630) before being plated into 24 well microplates (Greiner ...
-
bioRxiv - Cancer Biology 2021Quote: ... After 10 days constructs were fixed in 4% paraformaldehyde and embedded in paraffin and stained with H&E and stained for Fibronectin with FN1 antibody (F3648, Sigma Aldrich). For each construct the number of cells invading into the collagen was counted in at least five different fields of view under light microscopy ...
-
bioRxiv - Biochemistry 2021Quote: ... 0.01 mg aprotinin and 0.1 mM trans-epoxysuccinyl-L-leucyl amido (4-guanidino) butane (E-64) as a protease inhibitors (Sigma Aldrich®) and lysed by sonication (5 pulses for 1 min each ...
-
bioRxiv - Immunology 2021Quote: ... HEK293 and HEK293T cells (gifts from Caetano Reis e Sousa, The Francis Crick Institute, UK) were maintained in DMEM (Sigma-Aldrich) containing 4.5 g/L glucose ...
-
bioRxiv - Microbiology 2021Quote: ... PMH were cultured in hepatocyte culture medium (William’s E medium, GlutaMAX™ Supplement, Gibco™ 32551020; 100 U/ml penicillin/streptomycin, Sigma P4333 ...
-
bioRxiv - Molecular Biology 2022Quote: ... by negative selection and activated for 72 hr in the presence of LPS (50 μg/ml final concentration, E. coli 0111:B4; Sigma-Aldrich) plus IL4 (2.5 ng/ml final concentration ...
-
bioRxiv - Cancer Biology 2021Quote: ... The retrieved cells were seeded on collagen-treated vessels (at a density of 150,000 viable cells per cm2) and maintained in Williams’ medium E (Sigma, Darmstadt, Germany) supplemented with 1 μM dexamethasone ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were then incubated with 1 ml of SF DMEM containing the broad-spectrum hydroxamate metalloproteinases inhibitor CT1746 (100 μM) and the protease inhibitor cocktail (a mixture of aprotinin, bestatin, E-64, leupeptin and pepstatin A)(SIGMA, P1860) in the presence or absence (control ...
-
bioRxiv - Biophysics 2022Quote: ... The E protein was cleaved from the EGFP-8xHis fusion by incubation of the beads with 150 units of thrombin (Sigma-Aldrich) overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... MDMi cells were treated with 500 nM stable human Aβ1-42 oligomers (180222EMH, gift from Crossbeta) or 100 ng/mL lipopolysaccharide in PBS (LPS, L4391-1MG, E. coli 0111:B4, Sigma-Aldrich). As a control for the oligomer and LPS condition ...
-
bioRxiv - Neuroscience 2022Quote: ... Time-mated pregnant rhesus macaques at 127 days of gestation (80% of term gestation) were treated with intra-amniotic LPS 1mg (E. coli O55:B5, Sigma-Aldrich) diluted in 1 mL of sterile saline (LPS) ...
-
bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
-
bioRxiv - Plant Biology 2020Quote: ... As negative control one set of samples was pre-incubated with 20 μM E-64 (Sigma-Aldrich, St. louis, Mississippi, USA). Subsequently ...
-
bioRxiv - Biophysics 2021Quote: ... cell monolayers were allowed to migrate for at least four hours before exchanging the medium with pre-warmed medium containing 10 μg/ml E-cadherin blocking antibody (clone DECMA-1, Millipore MABT26).
-
bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
-
bioRxiv - Microbiology 2019Quote: Peptidoglycan-derived stem peptide fragments including ‘A-E-K-A-A’ pentapeptide (L-Ala-D-Glu-L-Lys-D-Ala-D-Ala (Sigma-Aldrich), ‘K-A-A’ tripeptide (acetyl-L-Lys-D-Ala-D-Ala ...
-
bioRxiv - Immunology 2020Quote: ... 180 uL of co-culture media (RPMI-1640 media containing 1% FBS, LPS (100 ng/mL; from E coli, Sigma-Aldrich) and IFNɣ (100 ng/mL ...
-
bioRxiv - Immunology 2020Quote: ... in the central-left abdomen area and 10 μg of LPS (from E. coli, serotype 055:B5 and deproteinized; Sigma-Aldrich) in 100 μl of PBS in the centralright abdomen area ...
-
bioRxiv - Neuroscience 2022Quote: ... media was aspirated and cells were exposed to 10/50 μg/mL Lipopolysaccharide (LPS; E. coli strain 0111:B4; Sigma-Aldrich) (MilliQ H2O vehicle ...
-
bioRxiv - Microbiology 2024Quote: ... and methyl N-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-N-methoxycarbamate (i. e. pyraclostrobin, pesticide analytical standard, CAS 175013-18-0, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
-
bioRxiv - Developmental Biology 2024Quote: ... the broad-spectrum hydroxamate metalloproteinases inhibitor CT1746 (100 µM) and the protease inhibitor cocktail (a mixture of aprotinin, bestatin, E-64, leupeptin and pepstatin A, 1/1000)(SIGMA, P1860) with or without 20 nM Wnt5a in the presence or absence of 500 nM RAP for 3 h at 37 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Central transverse sections of the heart were cut into 4 μm sections and stained with haematoxylin and eosin (H&E) and Masson’s Trichrome staining (#HT15, Sigma-Aldrich, UK) for collagen detection ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were stimulated for 4 h with one of the following: LPS (E. coli 0127:B8 100 ng/ml; Sigma-Aldrich); solutions of mix of synthetic neuromelanins (NM 10 μg/mL of Pheo-β-LG and 10 μg/mL of Pheo-β-fl) ...
-
bioRxiv - Neuroscience 2022Quote: ... media was aspirated and cells were exposed to 10 or 50 μg/mL lipopolysaccharide (LPS) (E. coli strain 0111:B4; Sigma-Aldrich) (MilliQ H2O vehicle ...
-
bioRxiv - Neuroscience 2023Quote: Induced microglia-like cells were treated for 6 and 24h with 100ng/ml of LPS from Escherichia coli (E. coli) O55:B5 (Sigma L2880) that was diluted first in DPBS and then added to the iMG media ...
-
bioRxiv - Immunology 2023Quote: ... Staining was done using hematoxylin and eosin (H&E) to view the alveolar spaces and inflammatory cells and Grocott’s Methenamine Silver stain (GMS) (Sigma-Aldrich, USA) to stain fungal cells in infected tissue ...
-
bioRxiv - Systems Biology 2023Quote: ... All E. coli strains (a gift from Dr. Christian Rudolph) were routinely grown in modified Luria Broth (LB) (1% tryptone (Sigma-Aldrich), 0.5% yeast extract (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2023Quote: ... either fresh or thawed DCs (differentiation day 8) were stimulated for 24 h with 200 ng/ml lipopolysaccharide (LPS; E. coli O26:B6, Sigma-Aldrich) and used for experiments on day 9 ...
-
bioRxiv - Cancer Biology 2023Quote: ... then incubated with primary antibodies (CK8, Abcam ab182875,1:200. CK14, Biolegend 905301,1:400; E-cadherin, Biolegend 147301,1:200; α-SMA, Sigma A5228, 1:500) diluted in blocking buffer at 4°C overnight ...
-
bioRxiv - Immunology 2023Quote: ... CD11c+ were purified and either plated with no further supplementation or stimulated with 100 ng/mL or 1 μg/mL of lipopolysaccharide (LPS, E. coli O111:B4, Sigma-Aldrich).
-
bioRxiv - Neuroscience 2023Quote: ... 8-week old male and female C57BL/6J mice (n=2/sex/condition) were intraperitoneally injected once with 1.0 mg/kg lipopolysaccharide (LPS; Lipopolysaccharides from E. coli O55:B5, Sigma-Aldrich L5418) or vehicle solution 1xPBS (Phosphate buffered saline ...
-
bioRxiv - Neuroscience 2023Quote: ... 8-week old male and female Cx3cr1-GFP mice bred on a C57BL/6J background (n=2 females and 1 male/condition) were intraperitoneally injected with 0.5 mg/kg lipopolysaccharide (LPS; Lipopolysaccharides from E. coli O55:B5, Sigma-Aldrich L5418) or vehicle solution (PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... 4a-e, 4g, 4j, 5d-f) were cultured in 24-well plates (Falcon, 353047) on mixed cellulose ester Millicell inserts (Millipore, PIHA01250). Cells were plated at 100,000 cells/insert and cultured 6-8 weeks before experimental manipulations ...
-
bioRxiv - Genetics 2023Quote: ... each probe set was added (final concentration of 500 nM) to 20mM VRC and hybridization buffer (1% Dextran sulfate [EMD Millipore], 1 mg/mL E. coli tRNA [Sigma] ...
-
bioRxiv - Cell Biology 2023Quote: Cells post aggregation and appropriate control (EGTA for E-cad and Cells without DNA labelling for DNA-cad) were incubated on ice with RIPA lysis buffer (Sigma Aldrich) supplemented with proteases and phosphatases inhibitor cockbTails (Sigma Aldrich) ...
-
bioRxiv - Neuroscience 2024Quote: Mouse vibrissae were carefully isolated from the upper lip pad of 4-week-old C57BL/6 male mice and cultured in Williams E medium (Sigma-Aldrich), supplemented with 1% (v/v ...
-
bioRxiv - Cell Biology 2024Quote: ... EBs at day 5 were transferred to a new 6-well plate coated with PDL (Millipore. Catalog. MILL-A-003-E)/Laminin (Gibco ...
-
bioRxiv - Immunology 2024Quote: ... respectively) were seeded in Seahorse XF 96 designated plates using 100 µg/ml poly-D-lysine (Sigma-Aldrich, A-003-E) and assayed according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... programed for uniform mixing as previously described at a controlled temperature and modified with online automated desalting.13 Proteins were denatured in a solution of 35% v/v 2-2-2 Trifluoroethanol (TFE, Sigma), 40 mM Dithiothreitol (DTT ...
-
bioRxiv - Neuroscience 2023Quote: ... mice were anesthetized by intraperitoneal injection with Avertin (2, 2, 2-Tribromoethanol, Sigma-Aldrich, St. Louis, MO, USA; catalog #T4). Cardiac perfusion was performed with 0.1 M phosphate-buffered saline (PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... the fluorescent dyes were removed by flowing 2 mL of the cleavage buffer containing 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; 646547, Sigma) with a 15-min incubation time in the flow chamber to cleave the disulfide bond linking the dyes to the readout probes ...
-
bioRxiv - Developmental Biology 2023Quote: ... The following factors were applied to experimental BPs in culture at the specified concentrations: 2-Deoxyglucose (2-DOG) 2 mM (SIGMA), Sodium Pyruvate (NaP ...
-
bioRxiv - Immunology 2023Quote: ... T cells transduced with the anti-hCD19 CAR construct 42 were cultivated for additional 2 days in presence or without 2 mM 2-DG (Sigma) before adoptive transfer into MC38-hCD19 tumor-bearing mice.
-
bioRxiv - Biophysics 2023Quote: ... different amounts of freeze dried GelMA macromer were dissolved in DPBS containing 0.5% (w/v) 2-Hydroxy-4’-(2-hydroxyethoxy)-2- methylpropiophenone (Sigma-Aldrich) as photoinitiator ...
-
bioRxiv - Immunology 2020Quote: ... for 2 days and then just IL-2 (20ng/ml) (Sigma-Aldrich) for a further 5 days ...
-
bioRxiv - Developmental Biology 2021Quote: ... the tissues were processed to 2’-2’-thio-diethanol (TDE, Sigma, 166782) for clearing and mounting ...