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Citations for Millipore Sigma :
1051 - 1100 of 10000+ citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1% human insulin (Sigma-Aldrich), and penicillin (100 I.U./ml ...
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bioRxiv - Immunology 2023Quote: ... A human IgG (Sigma #I2511) standard curve (ten three-fold serial dilutions starting at 20 µg/ml ...
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bioRxiv - Microbiology 2023Quote: ... 1% human insulin (Sigma-Aldrich), and penicillin (100 I.U./ml ...
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bioRxiv - Immunology 2023Quote: ... 2% human serum (Sigma-Aldrich), 1% non-essential amino acids (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... human insulin (I2643-25mg, Sigma) and monothioglycerol (M1753 ...
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bioRxiv - Immunology 2024Quote: ... A human IgG (Sigma #I2511) standard curve (10 three-fold serial dilutions starting at 20 µg/mL ...
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bioRxiv - Biophysics 2020Quote: ... Thrombin cleavage of the affinity tag was performed using the Sigma Thrombin CleanCleave Kit (Sigma) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were incubated with the antibodies as follows: antibodies for HSV tag (Millipore, 69171), SREBP-1 (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2019Quote: Selected oligonucleotides were ordered with a 5′-biotin tag and HPLC-purified from Sigma-Aldrich. Each oligo was diluted to 100 μM in nuclease-free 10 mM Tris pH 8 (diluted from Ambion 1 M Tris pH 8 ...
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bioRxiv - Physiology 2019Quote: ... flag epitope tag (Cell Signaling clone D6W5B, 14793S and Sigma clone M2, F3165 and A2220), GST (Cell Signaling clone 91G1 ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-TMIGD1 constructs in pFlag-CMV-1 (N-terminal Flag tag, Sigma-Aldrich, Munich, Germany): hTMIGD1 without signal peptide (AA 30-262 ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was cleaved by overnight incubation with thrombin (from bovine plasma, Sigma Aldrich) at 4 °C during dialysis ...
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bioRxiv - Bioengineering 2021Quote: ... The coding sequences for the His6-Tag were amplified with the pCOLADUETTM-1 (Merck/Millipore) plasmid backbone ...
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bioRxiv - Biophysics 2020Quote: ... FLAG and T7 affinity tags were detected with anti-FLAG (F3165, M2 clone, Sigma-Aldrich) and anti-T7 antibody (69522 ...
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bioRxiv - Microbiology 2020Quote: ... Wild-type and mutant VP40 bearing a Strep-Tag were cloned into pTriEx-5 (Novagen) and transfected into cells using TrasnIT-LT1 transfection reagent (Mirus) ...
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bioRxiv - Immunology 2022Quote: ... and 100 µL of alkaline phosphatase-conjugated anti-HIS tag antibody (Sigma-Aldrich, cat: A5588), diluted 1:2,000 in TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary anti-Flag tag antibody was obtained from Sigma-Aldrich (F7425-.2MG, WB 1:2000). Primary antibodies against viral proteins were obtained from ...
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bioRxiv - Bioengineering 2021Quote: ... then probed with the conjugated anti-6x His tag® mouse monoclonal antibody (Sigma-Aldrich) overnight at 4ºC or 1 hour at room temperature and rewashed three times with washing buffer (CMT ...
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bioRxiv - Microbiology 2022Quote: ... the primary antibodies were horse radish peroxidase-conjugated α-poly-histidine tag antibody (Sigma Aldrich; St ...
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bioRxiv - Physiology 2020Quote: ... the N-terminal-His-tag was labeled with the Ni-NTA Atto550 fluorophore (Sigma-Aldrich) following the protocol provided by the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... and the histidine tag removed with carboxypeptidase A bound to agarose (Sigma, St. Louis, MO) at a ratio of 10 protein:1 enzyme (w/w) ...
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bioRxiv - Biophysics 2022Quote: ... the N-terminal hexa-histidine tag was cleaved with ∼2 Units of bovine thrombin (Sigma) per 1 mg of protein ...
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bioRxiv - Molecular Biology 2023Quote: ... the sequence containing the HA tag were transferred in a bacterial expression vector pET22b (Novagen) between Xho1 and HindIII restriction sites ...
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bioRxiv - Cancer Biology 2023Quote: ... CUT&Tag analysis was assayed for the following epigenetic markers: H3K4Me2 (Millipore, Cat# 07-030), H3K27Ac (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... the histidine tag was then removed by proteolytic digestion with 1U/mg of thrombin (SIGMA). The digestion mixture was then loaded again on the IMAC on a Ni2+-His-Trap column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... full-length REC114 and mutants were cloned as Strep-tag fusions into pRSFDuet-1 (Novagen). MEI41-43 and its mutants were cloned as His-MBP fusions in pETM41 ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were probed at 4◦C overnight with mouse monoclonal anti-Myc-Tag antibodies (Sigma) at the 1:1000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated with a rabbit anti-HA-tag antibody (H6908, Sigma‒Aldrich) in PBS-Tx supplemented with 0.2% BSA ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with M2 antibody (anti-FLAG-tag, catalogue no. F1804A-5MG; Sigma) at 4 ℃ for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... 68] (dilution 1:1000) and mouse mAb to FLAG tag (Sigma, F3165, dilution 1:500).
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bioRxiv - Pathology 2020Quote: ... high-purity (>99%) human CRP obtained from human plasma (C4063; Sigma-Aldrich, Saint Louis, Missouri) was infused via the femoral vein after 45 minutes of ischemia with LAD ligation ...
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bioRxiv - Biophysics 2020Quote: ... Human Serum from human male AB plasma and NaOH 1N Bioreagent were purchased from Sigma. ss M13mp18 ...
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma-Aldrich) were used.
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bioRxiv - Molecular Biology 2020Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma Aldrich) were used.
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in CF media + 37.5mg/ml 70 kDa Ficoll (Fc; Sigma, F2878) and 25 mg/ml 400 kDa Ficoll PM (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Selective oxidation of hmC to fC was achieved using potassium perruthenate (KRuO4, Sigma-Aldrich) as described (Booth et al. ...
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bioRxiv - Neuroscience 2020Quote: ... 5% FCS with primary antibodies: anti-Calbindin D-28K (rabbit polyclonal, 1:1000, Millipore, AB1778 ...
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bioRxiv - Neuroscience 2021Quote: ... 17 div cultured neurons were incubated with TrkB-Fc (1 µg/mL, T8694-Sigma) a BDNF scavenger suppressing BDNF binding to TrkB (Sakuragi et al ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (vol/vol) FCS and 100 U/mL penicillin/streptomycin (Sigma Aldrich). D2.0R-GFP and 4T1-mCherry cells were generated by lentiviral transduction with TurboGFP and mCherry reporter ...
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bioRxiv - Cancer Biology 2022Quote: ... in RPMI-1640 medium complete (R8758, Sigma-Aldrich, supplemented with: 10% FCS (Sigma-Aldrich), 2mM L-glutamine (Biochrom) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10% heat inactivated Fetal Calf Serum (FCS) (Sigma-Aldrich, St. Louis, Missouri, USA) and under standard culture conditions in humidified incubator at 37°C with 5% CO2.
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bioRxiv - Cancer Biology 2021Quote: ... consisting of 1% fetal calf serum (FCS; #S0615, Sigma, part of Merck, Darmstadt, Germany) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... binding was detected by using goat-anti-hIgG(Fc)-HRP (1:70000, A0170, Sigma). Binding was visualized by using TMB substrate ...
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bioRxiv - Microbiology 2022Quote: ... RPMI-1640 or MEM media and 10% Fetal Calf Serum (FCS, Sigma cat. F7524). THP-1 cells media was also supplemented with 0.05 mM β-mercaptoethanol (Euromedex ...
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bioRxiv - Microbiology 2021Quote: ... Cell permeabilization and blocking was performed in IF buffer (5% FCS, 0.05% saponin (Sigma) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... and at day 13 with 50 μg of RBD-Fc plus Titermax adjuvant (Sigma). Blood were collected from individual mice one week after the last immunization (day 20 ...
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bioRxiv - Immunology 2021Quote: ... The cultures were supplemented on day 2 with FCS (10% v/v; Sigma-Aldrich). Medium was replaced every 3 days thereafter with fresh R+ containing FCS (10% v/v ...
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bioRxiv - Immunology 2020Quote: Cells were thawed and subsequently washed in IMDM+10%FCS+75U/ml DNAse (Sigma). To minimize day-to-day variation ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T and NIH-3T3 cells were grown in DMEM with 10 % FCS (Sigma-Aldrich, St ...