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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... C-terminal tags were removed by incubation overnight at 4° C with Thrombin (Sigma). Thrombin was removed by passage over benzamidine agarose beads ...
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bioRxiv - Biochemistry 2020Quote: ... C-terminal tags were removed by incubation overnight at 4° C with Thrombin (Sigma). Thrombin was removed by passage over benzamidine agarose beads ...
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bioRxiv - Cell Biology 2021Quote: ... Atg18 was isolated by affinity purification using the Gly6-FLAG3 tag and Dynabeads (Sigma) crosslinked with the FLAG M2 antibody (F1804 epitope ...
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bioRxiv - Plant Biology 2021Quote: ... Western blotting was performed using anti His-tag (Cat no. H1029-5ML, Sigma, USA) as well as with anti GST antibody (Cat no ...
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bioRxiv - Microbiology 2020Quote: PfaF (locus tag: PBPRA0221) was cloned into pET28 as an NheI-XhoI fragment (Novagen) as to generate N-terminal 6xHis tagged protein ...
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bioRxiv - Microbiology 2020Quote: ... A horseradish peroxidase-labeled anti-His tag antibody (1:25000 dilution; Sigma-Aldrich, Poland) diluted in 5% skimmed milk / TBS-Tween (0.1% ...
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bioRxiv - Microbiology 2021Quote: ... The results were analyzed by immunoblotting using antibodies for the Flag tag (SIGMA, SLCD6338).
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bioRxiv - Neuroscience 2020Quote: ... The His-tag was removed by O/N incubation with TEV protease (Millipore Sigma) at 4 °C followed by negative INAC ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Cell Biology 2022Quote: ... FTractin cDNA was fused with Halo tag in pTriEx-4 vector (Millipore Sigma, MA). EGFP was swapped with mEos3.2 41 in the cortactin-EGFP vector to obtain cortactin-mEos3.2 ...
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bioRxiv - Biochemistry 2019Quote: The TAP tag was detected using Peroxidase-anti-peroxidase 1:5000 (Sigma, Lot #103M4822), Pgk1 was detected using mouse anti-Pgk1 1:7500 (459250 ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tag was removed by treating purified nanobody Nb_0 with carboxypeptidase A (Sigma) and B (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Expression of pAgos was verified by Western blot with antibodies against His-tag (Sigma) after cell growth for 2 hours at 30 °C ...
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bioRxiv - Physiology 2023Quote: ... TAG levels were then measured using the Serum Triglyceride Determination Kit (TR0100-1kt, Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Monoclonal ANTI-FLAG® M2 antibody used in Cut&Tag assay was from Sigma. Mouse CD4 T Cell Isolation Kit used for the enrichment of CD4+ T cells was from Stem cell ...
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bioRxiv - Biochemistry 2023Quote: ... REC114 variants were cloned as N-terminal Strep-tag fusions into pRSFDuet-1 (Novagen) and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 2h with antibodies recognizing the FLAG-tag (F-1804; Sigma-Aldrich), Strep-tag (NBP2-41073 ...
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bioRxiv - Physiology 2023Quote: TAG and total cholesterol evaluation were processed according to the manufacturer’s instructions (Sigma-Aldrich, MAK266 for TAG and Cell Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Genetics 2024Quote: ... These antisense DNA probes were synthesized with Biotin-TAGs at the 5’ ends (Sigma) and 100 µM probes were pooled in equimolar ratios ...
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bioRxiv - Molecular Biology 2024Quote: ... and a C-terminal His6-tag was cloned into the expression vector pET21b (Novagen). The MALT1(PCASP-Ig3)339–719-his construct was transformed into Escherichia coli strain T7 express competent cells ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... TAG content was quantified using the High Sensitivity Triglyceride Assay Kit (Sigma-aldrich MAK264), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was carried out in freshly made blocking buffer (5% FCS, Sigma-Aldrich, Gillingham ...
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bioRxiv - Immunology 2022Quote: ... in 10 ml VLE DMEM (Biochrom) containing 10% heat-inactivated FCS (Sigma-Aldrich), 0.1% 2-mercaptoethanol (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Spleens were gently meshed in FACs buffer (PBS containing 3% FCS from Sigma) using a 40μM cell strainer to prepare single cell suspensions ...
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bioRxiv - Molecular Biology 2019Quote: ... MDCKII cells were grown in DMEM + 10%FCS + Antibiotic/Antimycotic solution (Sigma A5955)(Williams et al. ...
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bioRxiv - Genomics 2021Quote: ... supplemented with 10% FCS tested to be dox-free (Sigma-Aldrich, Taufkirchen, Germany) and penicillin and streptomycin (final concentrations 100 units/ml and 100µg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...
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bioRxiv - Plant Biology 2022Quote: ... and anti-Mouse IgG (Fc-specific)-Peroxydase (Sigma, dilution: 1/10000, 1.5hr incubation). Anti-Hsp90 (Agrisera ...
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bioRxiv - Immunology 2020Quote: ... the media was replaced by FCS-free medium containing 0.1% BSA (Sigma-Aldrich) and cells were stimulated with 100 μl milk EVs or EV-depleted controls in the presence or absence of 5 μg/ml Poly I:C (Invivogen) ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO, USA), 10mM HEPES ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected in each matrix 25 μg of Recombinant Sema3C-Fc (Sigma-Aldrich) in a concentration of 50 μg/ml in PBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... cell media was supplemented with 500 μg/mL 5-FC (Sigma, MO, USA). For control conditions ...
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bioRxiv - Immunology 2019Quote: ... before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich], 90% FCS [Sigma-Aldrich]). This method preserves immune cell viability ...
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bioRxiv - Immunology 2019Quote: ... before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich], 90% FCS [Sigma-Aldrich]). This method preserves immune cell viability ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Biotinylated goat anti-mouse IgG (diluted 1:500 in 10% FCS) (Sigma-Aldrich) was added to the wells and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2021Quote: ... sBHI media controls or Porphyromonas cell-free supernatants (240 µg of total protein) were incubated with 120 µg of human fibrinogen (Sigma-Aldrich, St. Louis, MO). Reaction mixtures were incubated at 37°C under anaerobic conditions or in an atmosphere of 5% CO2 for cell suspension or cell-free supernatants ...
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bioRxiv - Cell Biology 2020Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45µm filter (EMD Millipore), and used for transduction of 4T07 ...
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bioRxiv - Molecular Biology 2021Quote: ... After 2h of virus adsorption in the presence of 0.2 mg/ml of porcine pancreatin (Sigma-Aldrich) prepared in CMGF(-) ...
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bioRxiv - Cell Biology 2019Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2µg/ml Puromycin (Sigma-Aldrich). After 7 days of selection ...
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bioRxiv - Microbiology 2020Quote: ... Airway Chips infected with influenza virus (MOI = 0.1) were treated with 1 µM oseltamivir acid (Sigma-Aldrich) under flow (60 µl/h ...
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bioRxiv - Cell Biology 2021Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45μm filter (EMD Millipore) and used for transduction of MCF-10A cells in the presence of polybrene (8μg/mL) ...
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bioRxiv - Neuroscience 2021Quote: Each animal (with DREADD virus injection) received CLZ (0.5 mg/kg, Sigma-Aldrich, dissolved with 0.1% DMSO) or vehicle (sterilized saline with 0.1% DMSO ...
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bioRxiv - Immunology 2022Quote: ... the antibody-virus mixture was removed and 100 μl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cancer Biology 2022Quote: ... pLVX-EF1α-mKate2-WPRE-Neo virus supplemented with 10 µg/ml polybrene infection/transfection reagent (Sigma-Aldrich) was incubated with PSCs grown in monolayer for 24 h ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... virus was also concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore). Mice were injected s.c ...