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Citations for Millipore Sigma :
1001 - 1050 of 3415 citations for Human ADH5 siRNA since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Human IL-8 ELISA Kit (RAB0319, Sigma Aldrich), Human TNF Alpha ELISA Kit (ab181421 ...
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bioRxiv - Bioengineering 2024Quote: ... Human IL-6 ELISA Kit (RAB0306, Sigma Aldrich), Human IL-8 ELISA Kit (RAB0319 ...
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bioRxiv - Biophysics 2024Quote: ... and 0.4 ug/mL human insulin (Sigma 19278). After three days ...
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bioRxiv - Genomics 2024Quote: ... 200µg/mL human holo-transferrin (Sigma-Aldrich, T0665), 1ng/mL recombinant human IL-3 (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/ml of human EGF (Sigma, E9644)/ 100 ng/ml Noggin (Cerderlane labs ...
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bioRxiv - Cell Biology 2024Quote: ... followed by blocking with human IgG (Sigma, I4506) at a concentration of 5 μg per 1 x 10⁶ cells on ice for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µg/mL of human insulin (#I9278, Sigma), 100 µM nonessential amino acids (#11140050 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2.5 μg/ml human recombinant insulin (Sigma)) ...
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bioRxiv - Developmental Biology 2024Quote: ... 2.5 μg/mL human insulin (Sigma-Aldrich, I9278), 50 μM 2-mercaptoethanol (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... Mature human white-like adipocytes were transfected in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522) to mimic the subcutaneous tissue environment ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG (Jackson Immuno Research 109-036-088) or anti-human IgA (Sigma A0295) secondary antibody conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection for mature human white-like adipocytes was performed in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522). Both HeLa cells and mature human white like adipocytes were transfected with LNPs at a final mRNA concentration of 1.25μg/mL ...
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bioRxiv - Microbiology 2024Quote: ... The human ORF sequence encoding for GSDMD was obtained from the Viral Vector Facility (LUMC) human ORF (cDNA) library from Sigma-Aldrich. The gene ORF was obtained in pDONR223 entry vectors and amplified by PCR with overhang primers to introduce partial sequences of an N-terminal FLAG-tag and C-terminal Myc-tag ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Pathology 2023Quote: ... adiponectin levels were measured by enzyme-linked immunosorbent assays (Human Chemerin ELISA kit, Biovendor, Brno, Czech Republic; Human Resistin ELISA kit, Millipore Sigma; Human Total Adiponectin/Acrp30 Quantikine ELISA Kit ...
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bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords using collagenase type I (Sigma-Aldrich, 400 U/mL) to separate the cell layer surrounding the vein lumen ...
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bioRxiv - Cell Biology 2024Quote: ... Human EndoC-βH3 cells (Human Cell Design) were cultured on 2 µg/mL fibronectin and 1% extracellular matrix (E1270, Sigma-Aldrich) coated plates in Advance DMEM/F-12 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... of rat embryonic fibroblasts (REF) cells were transferred (seven days post initial siRNA tranfection) onto REF feeder layer inactivated with mitomycin C (Sigma) in replicates of four in a 12 well plate and maintained in ESC media (DMEM/F12 supplemented with 20% knockout serum replacement (KSR ...
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... A total of 2.5 μg of plasmid DNA or 80 ng of siRNA (siWIPI3, EMU081491 or siWIPI4, EMU007321 or siControl, SIC001 all Sigma) was used per transfection ...
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bioRxiv - Cell Biology 2021Quote: ... X-tremeGENE 9 DNA Transfection Reagent and X-tremeGENE siRNA Transfection Reagents were purchased from Sigma-Aldrich (St. Louis, MO). FG4592 (#HY-13426 ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transfection was carried out in a total of 15,000 ELT3-V cells transfected with SCR and Etv2 siRNA for 24 hours using X-tremeGENE HP DNA transfection reagent (Sigma). Cells were transfected with 10 ng of pRL_CMV vector (internal control ...
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bioRxiv - Cell Biology 2020Quote: Silencing of MASTL and TSC2 was performed using pLKO.1 lentiviral plasmids encoding specific siRNA (ON-TARGET SMARTpool, Dharmacon) or shRNA sequences (Sigma), as previously described 59 ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 nM siRNA against DDX3X (5’-CCUAGACCUGAACUCUUCAGAUAAU dTdT-3’) or 20 nM siRNA against GRSF1 (5’-GUGCCUCUCUGCUGCCGCAdTdT-3’) which were synthesized by Sigma. Cells were subsequently incubated at 37 °C for 48 h before harvesting and analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Specific depletion was controlled with an equivalent amount of non-targeting siRNA (D-001810-01-05, onTarget Plus, Dharmacon) or GFP esiRNA (EHUEGFP, Sigma). siRNA-induced depletion of vimentin was obtained with 20 nM of siRNAs from Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... per 2.7 x 105 hiPSC-CMs 5 days post plating using Mission® siRNA Transfection Reagent (Cat. S1452, Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: PFKFB3 depletion was carried out in HEK293 cells using siRNAs (80 nM; transfected twice on consecutive days) from SIGMA (SASI_Hs01_00065120). The cells were treated with control or specific siRNA and lipofectamine 2000 (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Immunology 2024Quote: ... the wells were incubated with PBS (control) or serially-diluted irrelevant human IgG (Human IgG isotype control, Sigma-Aldrich, St. Louis, MO) #1139c or #1193c (0.4 nM ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... and then treated with human complement serum (33.3%, Sigma) at 37oC for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml human insulin (Sigma-Aldrich, Gillingham, UK), 0.4 μg/ml hydrocortisone ...
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bioRxiv - Microbiology 2020Quote: ... the working volume of human serum (Sigma-Aldrich, Australia) was divided in half ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...
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bioRxiv - Genetics 2021Quote: ... 10 μg ml-recombinant human insulin (Sigma, 19278-5ML), 2 IU ml-heparin (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... supplemented with 1.25 mg/ml human insulin (Sigma #I9278), 1 % Penicillin-Streptomycin (Sigma #P4333 ...
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bioRxiv - Neuroscience 2022Quote: ... human basic fibroblast growth factor (bFGF) (Sigma, cat. #F0291), HEPES 1M (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 ng/mL of human bFGF (Sigma-Aldrich). Cells were incubated at 37 °C in a humidified atmosphere with 5% of CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... Normal Human Dermal Fibroblasts (NHDFs) (Sigma-Alrdrich, Buchs, Switzerland), a non-cancerous human skin fibroblast cell line ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10 % heat-inactivated pooled human serum (Sigma) and 100 ng/ml macrophage colony stimulating factor (R&D systems) ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgG (Fab specific) – peroxidase-conjugated antibody (Sigma) diluted 1:5000 in blocking buffer was added to the wells and incubated for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... or shRNA directed against human IP6K1 (TRC0000013508, Sigma-Aldrich) were co-transfected with VSV-G and psPAX2 (a gift from Didier Trono ...
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bioRxiv - Microbiology 2021Quote: Human neuronal progenitor cells (EnStem-A™ SCC003, Millipore) were cultured on plates previously cultured with geltrex (A1413201 ...
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bioRxiv - Immunology 2020Quote: ... with 5% human serum (Sigma-Aldrich; H5667 or H4522), 1% penicillin/streptomycin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in human serum from male AB plasma (Sigma) and incubated for 16 h at 37 □C with shaking (180 rpm) ...
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bioRxiv - Bioengineering 2020Quote: Human monocytic THP-1 cells (Sigma Aldrich, lot# 16K052) were cultured and expanded in suspension at a cell density of (0.5–1.5)·106 cells·ml−1 culture medium (RPMI-1640 containing 10 % FBS and 1 % P/S ...
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bioRxiv - Bioengineering 2020Quote: ... or human hemoglobin (all proteins were purchased from Sigma). Protein nanorods were prepared using chemically modified human serum albumin.
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bioRxiv - Neuroscience 2021Quote: ... human leukemia inhibitory factor (hLIF, 10 ng/ml, Millipore), SB431542 (2 mM) ...