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Citations for Millipore Sigma :
1001 - 1050 of 10000+ citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and membrane blocked overnight while shaking at 4°C in 5% milk (Marvel) containing 0.077% sodium azide (Sigma Aldrich). Primary antibodies were diluted in 5% milk/PBS/0.1% TWEEN 20 at concentrations as follows ...
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bioRxiv - Plant Biology 2021Quote: ... Pellet was washed twice with acetone at maximum speed (13,000xg) for 5 minutes at 4◦C and then air dried and resuspended in 50 mM ammoniumbicarbonate (ABC) (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated at 37 °C and 5% CO2 for 4 days before addition of resazurin (Sigma Aldrich) to a final concentration of 20 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Dehydrated tissue was then bleached with gentle shaking at 4°C for 48 hours in 1.5 ml of 5% H2O2 (Sigma) in methanol (1 volume of 30% H2O2 in 5 volumes of 100% methanol ...
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bioRxiv - Microbiology 2021Quote: ... centrifuged for 5 min at 16,000 g at 4°C and homogenized with glass beads (≤ 106 μm; G4649, SIGMA) in 350 μl of lysis buffer (RNeasy Plus Mini Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Conditioned media were retrieved by spinning down at 2,000 × g for 5 minutes at 4 °C then supernatants were passed through 0.22 μm filter (Millipore: SLGP033RS).
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bioRxiv - Immunology 2022Quote: ... overnight at 37°C in 5% CO2 tissue culture incubator and were subsequently fixed with 4% paraformaldehyde (Sigma-Aldrich) in DPBS for 30 minutes at RT ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular ions were extracted from dried pellets by overnight incubation at 4°C in 5 ml milliRho water (Millipore). Na+ and K+ were measured by flame spectroscopy with a Solaar spectrometer.
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bioRxiv - Microbiology 2022Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξCellLytic B (Sigma-Aldrich), 1 % Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... Immunoprecipitation was carried out by rotating sonicated lysates overnight at 4 C with NFIA antibody (5 mg, Sigma, HPA006111) or SOX9 antibody (EMD Millipore ...
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bioRxiv - Systems Biology 2022Quote: ... The lysate was centrifuged at 4,000 xg for 5 min at 4°C and the protein pelletwas resuspended in Digestion Buffer(10% 2,2,2-Trifluoroethanol (Sigma; #96924)) in 100 mM HEPEs pH 8.5) ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed once with TBS pH 8.0 for 5 min and incubated for overnight at 4 °C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Cell Biology 2023Quote: ... pelleted by centrifugation (5 min, 400 gavg, 4°C) and washed in phosphate buffered saline (PBS, Sigma, D8537-500ml), before being pelleted again ...
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bioRxiv - Biochemistry 2024Quote: ... Transferred PVDF membranes were blocked in 5% milk powder in 0.2% Tween-TBS overnight at 4°C and immunoblotted with mouse anti-FLAG-HRP (Sigma) conjugated primary antibody ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then incubated overnight at 4°C with primary antibodies diluted in blocking solution [5% Goat Serum (Sigma Aldrich) + 0.2% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 16,000 g for 5 min at 4 °C before it was immobilized on anti-FLAG (Sigma) agarose bead slurry for 30 min at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: Membranes were incubated overnight in primary antibody o/n at 4°C: Rabbit ⍺-Vang (Rawls & Wolff, 2003) diluted 1:1,000 in 5% milk in TBST or Rabbit ⍺-Actin (Sigma) diluted 1:2,000 in 10% Seablock in TBST followed by 3x wash in TBST for 10 minutes each ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei pellets were then collected by centrifuge for 4 min at 1,300 × g at 4°C and washed once in PBS supplemented with 5 mM sodium butyrate (Sigma) before binding to prepared concanavalin A coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked overnight at 4°C in 5% BSA (SRL) in 1X PBS containing 0.05% Tween20 (Sigma) (wash buffer) ...
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bioRxiv - Molecular Biology 2024Quote: ... Extracts were incubated overnight at 4 °C with 5-7.5 μl packed-volume of either M2 FLAG agarose beads (Millipore-Sigma), anti-SF3A2 Protein G-agarose beads ...
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bioRxiv - Bioengineering 2024Quote: ... the solution was pre-warmed to 40 °C and mixed 4:1 with freshly prepared 5 mg/mL lithium phenyl-2,4,6 trimethylbenzoylphosphinate (LAP, Sigma-Aldrich) in PBS (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... 4°C for 5 minutes before being resuspended in ALDEFLUOR™ assay buffer containing 0.2 μM DAPI (Sigma-Aldrich). Cells were sorted using a FACS-ARIA II (BD Biosciences) ...
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bioRxiv - Cell Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies diluted in 5% bovine serum albumin (BSA) (Sigma) in TBST ...
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bioRxiv - Neuroscience 2024Quote: ... BrdU was dissolved in 0.9% NaCl and injected at a concentration of 100 mg/kg 3 times each 2h (Sigma-Aldrich, Buchs, Switzerland). CldU was dissolved in 0.9% NaCl and injected at a concentration of 42.5 mg/kg either 3 times each 9h for the experiment about quiescence exit in vivo ...
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bioRxiv - Biochemistry 2020Quote: ... C-terminal tags were removed by incubation overnight at 4° C with Thrombin (Sigma). Thrombin was removed by passage over benzamidine agarose beads ...
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bioRxiv - Biochemistry 2020Quote: ... C-terminal tags were removed by incubation overnight at 4° C with Thrombin (Sigma). Thrombin was removed by passage over benzamidine agarose beads ...
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bioRxiv - Cell Biology 2021Quote: H2S production was measured using 7-Azido-4-Methylcoumarin (AzMC) fluorescent dye (Sigma, #802409). The protocol was adapted from Szabo Laboratory with minor modifications (Modis et al. ...
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bioRxiv - Cell Biology 2024Quote: ... 4 to 7-day-old protonemata were digested with 0.5% (w/v) driselase (Sigma) in 8.5% mannitol and regenerated on PRMB medium (supplemented with 100 µg/ml vancomycin ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were then chemically cross-linked to the membrane during 90 min at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma Aldrich). Membranes were prehybridized for 30 min in PerfectHyb™ plus (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... in 0,5× TBE buffer for 1h at 350 mA of current and EDC-cross-linked for 45 min at 60°C with a solution containing 33 mg/ml of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Sigma Aldrich), 11 ng/ul of 1-methylimidazol and 0.46% of HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... minced using sterile razor blades and digested in a 5 mL tube containing 3-5 mL of the digestion mix at 37 °C for 90 minutes: Collagenase type V (Sigma-Aldrich C9263; 5 mg/mL) and Dispase II (Gibco 17105041 ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then co-labelled overnight at 4°C with primary antibodies directed against Paired Box 7 (Pax7; DSHB; 1:50) and Laminin α1 (Sigma-Aldrich #L9393; 1:100) in 2% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then co-labelled overnight at 4°C with primary antibodies directed against Paired Box 7 (Pax7; DSHB; 1:50) and Laminin α1 (Sigma-Aldrich #L9393; 1:100) in 2% BSA ...
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bioRxiv - Microbiology 2023Quote: ... The rest of the pre-cleared lysates was then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at a 1:400 dilution ...
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bioRxiv - Microbiology 2024Quote: ... Cells were centrifuged at 3500 x g for 7 min at 4°C and were resuspended in 100 µL RIPA buffer [50 mM TRIS-HCl (Sigma Aldrich, Cat. No. T1503), 150 mM NaCl (VWR ...
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bioRxiv - Immunology 2021Quote: ... DNA was counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). NETs were visualized by using an Olympus IX81 motorized epifluorescence microscope (Olympus America Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Pictures were acquired using a FSL confocal microscope (Olympus).
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bioRxiv - Immunology 2020Quote: ... Nuclei were counterstained with 4’,6’-Diamidino-2-phenylindole (DAPI; #D9564; Sigma) diluted 1/1500 in PBS/Ca/Mg for 5min ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Imaging of cells was carried out in epifluorescence and differential interference contrast (DIC ...
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bioRxiv - Neuroscience 2020Quote: ... Cell nuclei were stained with 4’,6-diamidino-2-Phenylindole (DAPI) (Sigma) to visualize cell layers and borders of the DCN and VCN ...
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bioRxiv - Neuroscience 2020Quote: ... DAPI (4′,6-diamidino-2-phenylindole; Sigma-Aldrich, North Ryde, NSW, Australia) was used for nuclear staining of the neurons.
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bioRxiv - Cell Biology 2021Quote: ... Nuclei were visualized with 4’,6-diamidino-2-phenylindole (DAPI, blue; Sigma) staining ...
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bioRxiv - Microbiology 2021Quote: ... samples were labeled with DAPI (4, 6-diamidino-2-phenylindole; Sigma-Aldrich) for 10 minutes at room temperature following fixation ...
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bioRxiv - Genetics 2021Quote: ... Nuclear staining was performed using 4’,6-diamidino-2-phenylindole dihydrochloride (Sigma). Then ...
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bioRxiv - Microbiology 2021Quote: ... labeling of nuclei using 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich) or ToPro3 (ThermoFisher ...
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bioRxiv - Physiology 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI, #D9542) was purchased from Sigma-Aldrich Co ...
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bioRxiv - Cancer Biology 2021Quote: ... then counter stained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Images were taken using an Olympus FV1000 confocal microscope (Tokyo ...
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bioRxiv - Cell Biology 2022Quote: ... and DAPI (4’,6-diamino-2-phenylindole) (D9542; Sigma; 1 μg/mL). Additionally ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was determined using 4’,6-diamidino-2-phenylindole (DAPI, Sigma). Flow cytometry was performed on a MACSQuant Analyzer 10 (Miltenyi Biotec) ...