Labshake search
Citations for Millipore Sigma :
1201 - 1250 of 10000+ citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2023Quote: ... The solubilized nucleoproteins were cleared by centrifugation at 20,400xg for 5 min at 4°C followed by filtration using an Ultrafree-MC spin column (Millipore, UFC30GV0S).
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were incubated O/N at 4°C followed by two washing steps of 5 minutes with Wash Buffer A (Sigma) at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... The fluid was centrifuged at 500xg for 5 min at 4°C and resuspended in RBC lysis buffer (Sigma, R7757) for 5 min at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... the cells were firstly cultured for 4 h in a 37°C/5% CO2 incubator with 50 ng/ml PMA (Sigma) and 1 mM ionomycin (Sigma ...
-
bioRxiv - Cell Biology 2022Quote: ... The conditioned media was spun down for 5 minutes at 800g and 4°C and then filtered with a syringe and 0.22 μm filter (Millipore, SLGV033RS) and stored at 4°C for no more than three days before being applied to cells ...
-
bioRxiv - Molecular Biology 2023Quote: The sorted cells were concentrated by centrifugation at 300 x g for 5 minutes at 4 °C and resuspended in DPBS (Sigma) with 0.04% w/v BSA (Sigma) ...
-
bioRxiv - Microbiology 2024Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξ CellLytic B (Sigma-Aldrich), 1 % Triton X 100 ...
-
bioRxiv - Immunology 2024Quote: ... at 37°C in pre-warmed epithelial cell removal solution (HBSS supplemented with 4% iHS [Sigma] and 5 mM EDTA [Sigma]). The remaining tissues containing lamina propria and muscularis layer were digested for 20 min digestion ...
-
bioRxiv - Molecular Biology 2024Quote: ... Extracts were incubated overnight at 4 °C with 5-7.5 μl packed-volume of either M2 FLAG agarose beads (Millipore-Sigma), anti-SF3A2 Protein G-agarose beads ...
-
bioRxiv - Plant Biology 2024Quote: ... Twenty µl was saved as input and the remaining supernatant was then incubated at 4°C overnight with specific antibodies (5 µg of H3K27me3 Ab, Diagenode C15410069 or Millipore 07-449 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, purity > 98%) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
-
bioRxiv - Cell Biology 2021Quote: ... 50 μL of azino-bis-3-ethylbenthiazoline-6-sulfonic acid (ABTS liquid substrate; Sigma) was added and incubated in the dark at room temperature for 20 minutes and 50 μL of 1% SDS was used to stop the reaction ...
-
bioRxiv - Biochemistry 2020Quote: ... TsFpn was then concentrated to 3-6 mg/ml (Amicon 50 kDa cutoff, Millipore) and loaded onto a size-exclusion column (SRT-3C SEC-300 ...
-
bioRxiv - Microbiology 2020Quote: ... 100 μL of azino-bis-3-ethylbenthiazoline-6-sulfonic acid (ABTS liquid substrate; Sigma) was added and incubated in the dark at room temperature and 100 μL of 1% SDS was used to stop the reaction ...
-
bioRxiv - Microbiology 2020Quote: ... 50 μL of azino-bis-3-ethylbenthiazoline-6-sulfonic acid (ABTS liquid substrate; Sigma) was added and incubated in the dark at RT and 50 μL of 1% SDS was used to stop the reaction ...
-
bioRxiv - Microbiology 2023Quote: ... 540-69-2] (pH 3, adjusted with formic acid [Sigma Aldrich, 64-18-6]) and solvent B was acetonitrile [Sigma Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... 3 mM Na+pyruvate (Sigma-Aldrich; St. Louis, MO; CAS No. 113-24-6), 2 mM N-acetyl-L-cysteine (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... 6 uM pargyline and 50 uM (-)-epigallocatechin-3-gallate (EGCG) (all from Sigma Aldrich). For metabolic pathway analysis ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cordycepin was added to the 3’ end of in vitro transcribed mRNA by substituting 50 mM Cordycepin (3’-deoxyadenosine) 5’-triphosphate sodium salt (Sigma-Aldrich) in 10 mM Tris pH7 for ATP in a poly(A)-tailing reaction with a Poly(A ...
-
bioRxiv - Neuroscience 2023Quote: ... a shRNA sequence based on 5’-GCCTAAATGGTCAAGGAGATA-3’ as the sense nucleotide corresponding to the 3’UTR of mouse mDia1 (NM_007858.4; Sigma-Aldrich, Cat# TRCN0000108685) was designed as an oligonucleotide with overhangs (s ...
-
bioRxiv - Genomics 2023Quote: ... then quickly was cut to small pieces (1-3 mm) in petri dish with 3 -5 ml RMPI medium containing 0.05mg/ml TM Liberase (Sigma Aldrich, 5401119001) and 0.02 mg/ml DNAaseI (ThermoFisher ...
-
bioRxiv - Cell Biology 2020Quote: ... The supernatant was incubated at 4°C with Glutathione Beads (Sigma) for 4 hours ...
-
bioRxiv - Cell Biology 2021Quote: ... and incubated overnight (4°C) with 1.5 μ antibody (4G10 Millipore). Immune complexes were captured with 50 μ magnetic beads (Millipore ...
-
bioRxiv - Immunology 2021Quote: ... and lysed at 4° C in either RIPA buffer (Sigma-Aldrich) or self-made lysis buffers (either 50 mM Tris pH 7.5 ...
-
bioRxiv - Neuroscience 2020Quote: ... Huntington aggregates were stained at 4 °C over night (EM48, Millipore; 1:500 ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were fixed with prewarmed (37°C) 4% paraformaldehyde (Sigma-Aldrich) for 10 min and permeabilised by incubation with PBS containing 0.5% Triton X-100 and blocked with 5% BSA in PBS for 20 min ...
-
bioRxiv - Neuroscience 2022Quote: ... stained with 1’ ab for 12h at 4°C (EMD Millipore [ab144P ...
-
bioRxiv - Neuroscience 2020Quote: ... the cells were embedded in 4% agar at 45°C (Sigma) under the stereomicroscope and cut into 1-2 mm3 blocks ...
-
bioRxiv - Cancer Biology 2020Quote: ... overnight at 4°C and p85 (Millipore, Cat. 0619, 1:10000) and GAPDH (Santa Cruz ...
-
bioRxiv - Neuroscience 2022Quote: ... and stored at 4°C in 0.03% Sodium Azide (Sigma-Aldrich) in PBS until use ...
-
bioRxiv - Cancer Biology 2024Quote: ... The bacteria were sonicated at 4°C in 1% Sarcosyl (Sigma), followed by adding Triton X-100 (1%) ...
-
bioRxiv - Immunology 2024Quote: ... blots were probed overnight at 4°C with anti-LC3 (Sigma) and anti-actin (Cell Signalling ...
-
bioRxiv - Neuroscience 2022Quote: ... the cells were embedded in 4% agar at 45°C (Sigma) under the stereomicroscope and cut into 1-2 mm3 blocks ...
-
bioRxiv - Genetics 2022Quote: ... pelleted at 4°C and lysed in the RIPA buffer (Sigma) along with complete protease inhibitor cocktail (Roche ...
-
bioRxiv - Cell Biology 2024Quote: ... and fixed at 4 °C overnight using 3.2% glutaraldehyde (Sigma Aldrich). Subsequently ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde and the slide was mounted using a medium containing 4′,6-diamidino-2-phenylindole-DAPI (Fluoroshield, Sigma-Aldrich, USA). A fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera ...
-
bioRxiv - Genomics 2024Quote: ... slides were counterstained in 4’,6-diamidino-2-phenylindole (DAPI) solution and embedded in Mowiol 4–88 mounting medium (Sigma-Aldrich, USA).
-
bioRxiv - Molecular Biology 2021Quote: ... to YPD containing 5 mM 4-thiouracil (Sigma, 440736-1G). Cells were incubated with 4tU for 5 minutes before pelleting (one minute ...
-
bioRxiv - Neuroscience 2021Quote: ... 5 dpf larvae were fixed in 4% paraformaldehyde (PFA; Sigma) in phosphate-buffered saline (PBS ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich), and 0.5 μg/ml anti-CD180 (RP/14 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 4 µM 5-fluoro-2′-deoxyuridine (FldU, Sigma-Aldrich) were added to the cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml mouse recombinant IL-4 (Sigma-Aldrich) to allow B cell activation and class switch recombination to IgG1 ...
-
bioRxiv - Neuroscience 2022Quote: ... 100μm of 4-AP (Sigma-Aldrich, CAS#: 504-24-5) was washed on for 3 minutes (the amount of time it took to regularly induce 4-AP oscillations ...
-
bioRxiv - Developmental Biology 2023Quote: ... followed by fixation in 4% formaldehyde (EMD Millipore FX0410-5) in 1X PBS with Triton X-100 (0.3% ...
-
bioRxiv - Immunology 2024Quote: ... or 4 mM EGTA (CAS 67-42-5, Sigma-Aldrich) were added to buffers as outlined in the text.
-
bioRxiv - Cancer Biology 2024Quote: ... and 5 μL of thrombin (4 units/ml, Sigma, UK) were mixed ...
-
bioRxiv - Neuroscience 2024Quote: ... 5′-cyclic monophosphate (dibutyryl cAMP) (Sigma-Aldrich, 60-92-4) and 2 ng/mL GDNF (Pepro Tech ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were stained with DAPI before mounting with Mowiol (12 mL of 0.2 M Tris buffer pH8.5, 6 mL distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore).
-
bioRxiv - Neuroscience 2023Quote: ... Coverslips were shortly rinsed in distilled water and mounted using Mowiöl (12 ml of 0.2 M Tris buffer, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore). Samples were imaged directly or within the next 48h ...