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Citations for Millipore Sigma :
9951 - 10000 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... were saturated with 3% fatty acid free BSA (Sigma-Aldrich A8806) in PBS-Tween-20 0.1% for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... We used 3-amino-9-ethylcarbazole (AEC) (Sigma-Aldrich, Steinheim, Germany) to visualize protein binding ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 μL 3% hydrogen peroxide solution (Sigma, catalog #88597-100ML-F) was added to the solution followed by thorough mixing ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were treated with 50-70 µL blocking solution (5% normal donkey serum [NDS, ImmunoReagents, SP-072-V×10], 1% Bovine Serum Albumin (BSA, Sigma-Aldrich, A1595-50ML), 8.5 mL PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Western blots were probed using the following primary and secondary antibodies (incubated in PBS, 0.1% Tween20 and 5% milk): rabbit anti-Eps8L2 (Sigma-Aldrich #HPA041143, 1:500), mouse anti-Flag (Sigma-Aldrich #F1804 ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were then blocked with 5% milk or BSA in TBST and incubated with the indicated antibodies: mouse monoclonal anti-Tubulin (#T5168, Sigma, 1/10,000 dilution), rabbit anti-cleaved PARP (#9546 ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies were diluted in PBS containing 5% fetal bovine serum (Biological Industries Israel, 04-007-1A) and 1% or 0.1% Triton X-100 (Sigma Aldrich Israel, X-100), respectively ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... cells were co-stained with Hoechst 3342 (1 µg/ml, total cell count) and propidium iodide (5 µg/ml, Sigma-Aldrich, dead cells). Cells were imaged using the InCell 2200 System ...
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bioRxiv - Cancer Biology 2020Quote: ... the remaining plates were fixed daily from day 2 to day 5 (excluding day 1) prior to staining with crystal violet (Sigma-Aldrich, C0775-100G) for 3-5 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were blocked for 1 h in Tris-buffered saline (TBS) with 5% milk and 0.05% Tween 20 (Sigma-Aldrich, St. Louis, MO) and washed 3 times (5 min each ...
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bioRxiv - Microbiology 2020Quote: Proteolysis was performed using 2 μM of encapsulated or unencapsulated DypB in proteolysis buffer (100mM Sodium acetate pH 5, 2mM DTT) with or without the addition of 1 μM Cathepsin B (Sigma-Aldrich Cat# 219362). Reactions were run for 14 hours at 40°C followed by analysis of samples by SDS-PAGE using 4-20% Criterion™ polyacrylamide gels (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... The female was anaesthetized for 5 min in a solution of MS 222 (tricaine methane sulfonate 0.18 g L-1; Sigma - Aldrich Co, Missouri, USA). Blood was then sampled from the caudal artery using a 23-gauge needle in a 1ml TB syringe (Becton ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed with TBST three times for 5 minutes and incubated with a 1:25,000 dilution of horseradish peroxidase-conjugated anti-mouse (Sigma, Cat no. # A9044-2ML6) or anti-rabbit (Sigma ...
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bioRxiv - Neuroscience 2019Quote: Permeability of the BNB (perineurium): Evans blue albumin (EBA) was prepared in 5% BSA with 1% EB dye (Sigma-Aldrich, St. Louis, USA) in sterile distilled PBS and filtered through a 0.20 µm filter (Sartorius stedim Biotech GmbH ...
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bioRxiv - Molecular Biology 2020Quote: ... Seedlings were then stained in 1/10 MS containing 10 µg/ml of FDA (Sigma, PDA stock solution 5 mg/ml in DMSO) for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... Permeabilized cells were blocked with 5% BSA diluted in PBS for 4 h at 4°C and incubated overnight with pan-Cadherin antibody (Sigma, C1821, 1:500), MRP2 antibody (Sigma ...
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bioRxiv - Genetics 2019Quote: ... hiPSC-CMs were permeabilized with 0.1% Triton X-100 and incubated in 5% normal goat serum before overnight incubation with the primary α-actinin antibody (Sigma Aldrich, A7811) at 4C ...
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bioRxiv - Cell Biology 2021Quote: Binding of RBD to the surface of cells was measured by flow cytometry after incubation with increasing doses of human lactoferrin (0, 1, 5 and 10 mM) (Sigma Aldrich Cat#: L1294) in a final volume of 100 μL of culture medium ...
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bioRxiv - Neuroscience 2021Quote: ... free-floating sections were washed with 1X PBS three times (1×10 min, 2×5 min) and incubated for 10 min in 0.5% Thio-S (T1892; Sigma-Aldrich, St. Louis, MO) diluted in 50% ethanol ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with FACS buffer (2% FBS and 5 mM EDTA in PBS) prior to staining with antibodies and/or Calcofluor White M2R (Sigma, 1 μg/mL) in FACS buffer for 15-20min ...
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Comparing the appetitive learning performance of six European honeybee subspecies in a common apiarybioRxiv - Animal Behavior and Cognition 2021Quote: ... bees were trained with 30 % sucrose solution as unconditioned stimulus and reward and 5 µl of 1-hexanol as conditioned stimulus (Sigma Aldrich, Steinheim, Germany). During each conditioning trial ...
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bioRxiv - Cancer Biology 2021Quote: ... and cultured at 37 °C for 24 h, followed by various concentrations (0, 1, 2, 5, 10 µM) of lomitapide (Sigma Aldrich, Missouri, USA). The plate was incubated at 5 % CO2 at 37 °C for 24 h after treatment with lomitapide ...
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bioRxiv - Immunology 2020Quote: ... the Jurkat cells were transduced with TCR lentivirus at an estimated multiplicity of infection (MOI) of 5 with 1 μl of polybrene at a final concentration of 4 μg/ml (Sigma, St. Louis, MO). Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco ...
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bioRxiv - Immunology 2021Quote: TCRδCreER.ZsGreen or TCRδCreER.TdTomato mice were fed with TAM chow for 30 days followed by normal chow and 1 mg/ml 5-bromo-2’-deoxyuridine (BrdU, Sigma, St. Louis, MO) in drinking water containing 1% glucose (to reduce the taste aversion and increase the palatability of water ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked for one hour in 5% normal goat serum in PBST and incubated overnight with rabbit anti-p75NTR (Millipore 07-476, 1:1000) and phalloidin-Alexa Fluor® 488 (Invitrogen A12379 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells in dissociation reagent were incubated for 15-20 min at 37°C to ensure dissociation of cell clusters before adding 5 mL of DMEM/F12 containing 0.04% BSA and 10% ROCK-inhibitor (1/1000; Sigma-Aldrich; Cat. No. 1254), followed by resuspension by gentle pipetting ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked in 5% BSA diluted in PBS before being incubated overnight at 4°C with rabbit ɑ-MBNL1 antibody (1:1000, Millipore Sigma, ABE241) or mouse ɑ-RFP antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were incubated with primary antibodies diluted in 5 % BSA in 1x PBS: Tau1 clone 46 (1:500) mouse monoclonal IgG (Sigma-Aldrich, T-9450), GFP (H-140 ...
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bioRxiv - Immunology 2022Quote: ... Lymph nodes were transferred to digestion media (HBSS, 5% Fetal Calf Serum, 1% HEPES) containing 500U/mL of Collagenase D (Sigma-Aldrich, Cat 11088866001) and 20 mg/mL of DNAse I ...
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bioRxiv - Cell Biology 2022Quote: ... NIH/3T3 cells were infected with the corresponding lentiviruses (1/5 – lentivirus/cell culture medium) in the presence of 4 μg/mL polybrene (Sigma-Aldrich, cat# 107689). 4 days post-infection ...
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bioRxiv - Neuroscience 2024Quote: ... 5% CO2 in DMEM:F12 containing 1% pen-strep and 10% FBS on 5 poly-D-lysine (PDL; P7405- 5MG; Sigma-Aldrich, St. Louis, MO) coated T-75 flasks ...
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bioRxiv - Physiology 2023Quote: ... The minced tissue is placed in a 15 mL conical tube (USA Scientific #188261) and 5 mL of MS-EGTA buffer with 1 mg Trypsin (Sigma #T1426-50 MG) is added to the tube ...
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bioRxiv - Bioengineering 2024Quote: ... 7.5 × 105 MDCK-HA cells were overlayed on the transfected HEK293ts with 1 µg/mL of TPCK-treated trypsin (Sigma-Aldrich T1426-50MG). When approximately 70% of the cells had died ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were diluted in antibody buffer (0.2 % Tween 20, 10 µg/ml heparin, both Sigma-Aldrich, 1 % BSA, 5 % DMSO in PBS) at the following concentrations ...
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bioRxiv - Immunology 2022Quote: ... rhesus macaque HSPCs were mobilized from the bone marrow by treatment with granulocyte colony-stimulating factor (G-CSF) 10-15 mg/kg/day (Amgen, Thousand Oaks, CA) for 5 days and AMD3100 (plerixafor) 1 mg/kg (Sigma, St. Louis, MO) on the morning of the fifth day ...
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bioRxiv - Plant Biology 2023Quote: ... The endocarp was submerged in 1:4:5 v/v/v mixture of 30% hydrogen peroxide (HX0635-2; EMD Millipore, Burlington, MA, USA), distilled water ...
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bioRxiv - Neuroscience 2022Quote: ... The silanized ion-sensitive barrel tip (5% trimethyl-1-chlorosilane in dichloromethane)was filled with a potassium ionophore I cocktail A (60031 Fluka distributed by Sigma-Aldrich, Lyon, France) and backfilled with 100 mM KCl ...
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bioRxiv - Bioengineering 2023Quote: ... The following day the tissues were incubated in primary antibody diluted 1:100 in fresh block buffer and 5% (v/v) dimethyl sulfoxide (DMSO; Sigma; a penetration enhancer) at 37°C for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were then washed with PBST for 5 min and incubated with the following primary antibodies: GAPDH (EMD Millipore, #MAB 374, 1:2000), β-catenin (EMD Millipore ...
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bioRxiv - Immunology 2023Quote: ... tumors were resected and single cell suspensions were generated by incubating in a 5 mL solution of RPMI containing collagenase type IV (1 mg/mL, Sigma Aldrich Cat. C5138), Hyaluronidase Type V (0.1 mg/mL ...
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bioRxiv - Immunology 2023Quote: ... LNs were resected and digested in a solution of a 5 mL solution of RPMI containing collagenase type IV (1 mg/mL, Sigma Aldrich Cat. C5138) and DNase Type IV (20 U/mL ...
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bioRxiv - Developmental Biology 2024Quote: ... the cultures were washed 3 times in PBS for 5 minutes and incubated with Duolink Plus and Minus PLA probes (diluted 1:5) followed by Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma, cat. no. DUO92102), according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked with blocking buffer (TBS containing 0.1% Tween-20 with 5% w/v skim milk) and then incubated with the primary antibody (anti-FLAG M2 (Sigma-Aldrich, USA; #F1804) or anti-alpha-tubulin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... mice were treated with 3 minutes of peri-adventitial application of either 5 µL of peri-adventitial elastase (0.4 U/mL type 1 porcine pancreatic elastase, Sigma Aldrich, St. Louis, MO) or heat-inactivated elastase as control ...
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bioRxiv - Physiology 2024Quote: ... 60 min and blocked for 1 hour in 5% skim milk in Tris buffered saline plus 0.1% Tween-20 (TBST; #P1379, Sigma-Aldrich, North Ryde, Australia). Membranes were incubated at 4°C overnight in the primary antibody ...
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bioRxiv - Synthetic Biology 2021Quote: ... purified Sx-Cm-MOMP and Sx-CtE-MOMP proteins were spotted directly onto nitrocellulose membranes and incubated for 5 min before being blocked with 5% BSA (Sigma) and probing with the same primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were collected at appropriate time points from 5-8 week old hormone-primed (5 IU each, pregnant mare serum gonadotropin (Sigma) and human chorionic gonadotropin (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... Eggs were de-jellied in a cysteine solution (1 g of cysteine and 500 µl of NaOH 5 M in 50 ml MMR 0.1×) and transferred to ficoll 5% in MMR 0.45× (w/v) (Sigma-Aldrich, P7798). To target the neural crest population ...
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bioRxiv - Molecular Biology 2021Quote: The concentrated elute was dialyzed with buffer BC-50 (BC buffer with 50 mM NaCl and 5% glycerol) and loaded on a 5 mL HiTrap Q Fast Flow anion exchange chromatography column (Sigma) using an ÄKTATM Pure FPLC (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were mixed with 3 μl of 2μM fluorescent primer (D4-5′-TTTCTTTCCCCCTGGCCTT for the probed/control samples and D2-5′-TTTCTTTCCCCCTGGCCTT for the sequencing reaction, Sigma) and incubated for 5 minutes at 65°C ...