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Citations for Millipore Sigma :
9451 - 9500 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... were obtained from ATCC and cultured at 37°C and 5% CO2 in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum and 1× penicillin-streptomycin (Sigma, St. Louis, MO, USA).
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bioRxiv - Neuroscience 2020Quote: ... 1×105 cells were plated on coverslips that were pre-coated with 5 μg/ml poly-D-lysine (Sigma-Aldrich, Rehovot, Israel) in a 24-well plate containing 1.5 ml Neurobasal-A medium (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM 2-mercaptoethanol) and concentrated up to 1 nmol/ml (1A260 = 69 pmol) using Amicon Ultra centrifugal filters (Millipore, MWCO 100,000). Further ...
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bioRxiv - Cancer Biology 2021Quote: ... The membranes were blocked in 5 % milk in 1XTBST and immunoblotted with primary antibodies against KYAT1 (1:1000, anti-CCBL1, #C4622) (Sigma, Darmstadt, Germany) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated with the primary antibodies in a blocking solution for 5 days at 4°C: mouse anti-GFAP (G3893, Sigma, 1:300), mouse anti-HSP70 (MA3-028 ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed five times in PBS-T and blocked with buffer containing 5% milk (Difco #232100) and 1% BSA (Sigma #A906-500G) in PBS-T (BioVision #2310-100) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 hour of blocking with 5% horse serum in PBS was followed by incubation with anti-FLAG primary antibody (1:500, Sigma Aldrich F3165) in 3% horse serum in PBS for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidified chamber at RT for 1 hr with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidity chamber at RT for 1 hour with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were subjected to blocking in 5% milk in TBST and incubated overnight with respective primary antibodies - rabbit anti-HA (1:1500, Cat. No. H6908, Sigma-Aldrich, USA), rabbit anti-c-fos (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in blocking solution (5% bovine serum albumin in PBST) for 60 min at room temperature and then incubated with anti-CB1R (Merck Millipore, 1:1000) in blocking solution overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μl 1x PBS and 2 μl (1 U) PNGase F (from Elizabethkingia meningoseptica, expressed in E. coli, Sigma Aldrich F8435-50UN) were added to 40 μl of SEC purified YFP-S-RBD (0.24 mg/ml protein concentration) ...
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bioRxiv - Neuroscience 2020Quote: ... and FoxP2-Cre mice using glass capillary (tip diameter 35 μm; 1-5 μL Hirschmann® microcapillary pipette, CAT#Z611239, Sigma-Aldrich) connected to a Picospritzer® pressure system (Parker Hannifin) ...
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bioRxiv - Plant Biology 2021Quote: Infiltrated leaf tissue was harvested 72 h later and proteins were extracted by grinding leaf tissue in liquid nitrogen followed by resuspension in extraction buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% Glycerol, 1 mM EDTA, 5 mM DTT, 1x protease inhibitor cocktail [P9599, Sigma], 10 μM MG132) at a ratio of 2 mL / g FW ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in TBS-T followed by incubation in the following primary antibodies that were diluted in TBS-T containing 5% milk: rat-anti-HA 3F10 (Sigma, 1:1,000) and rabbit-anti-aldolase (14 ...
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bioRxiv - Bioengineering 2019Quote: Samples were taken from scaffolds using a 2mm biopsy punch (n=5) and incubated with 1% alizarin red s (Sigma Aldrich, A5533) in distilled water for 10 min at a pH of between 4.1 - 4.3 ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated for 30 min at room temperature in PBS-T containing 5% milk powder and secondary antibody: peroxidase-conjugated anti-mouse IgG (1:5000) (Sigma-Aldrich, A2304). The membranes were then washed in PBS-T ...
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bioRxiv - Microbiology 2019Quote: ... 8 female CD-1 mice aged ∼5 weeks were randomly divided into two groups and immunosuppressed by adding disodium dexamethasone 21-phosphate (Sigma, cat. D1169) to drinking water at a concentration of 16 mg/L [29] ...
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bioRxiv - Biochemistry 2019Quote: ... 5 × 106 cell equivalents were analyzed on 10% PAA / SDS gels and sequentially probed with anti-HA antibodies (Sigma, 3F10, 1:3000), anti-Protein A antibodies (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... the membranes were washed 3x in TBS-T for 5 min each and incubated for 1 h with peroxidase-conjugated anti-rabbit secondary antibody (Sigma, cat # A0545) diluted 1:10,000 in TBS-T + 1% BSA solution ...
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bioRxiv - Immunology 2020Quote: ... and 50 μL supernatant was then mixed with equal parts Griess reagents A (dH2O with 1 % sulphanilamide [Sigma-Aldrich] and 5 % phosphoric acid [Sigma-Aldrich]). The mixture was incubated in the dark for 10 min at room temperature (RT) ...
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bioRxiv - Plant Biology 2020Quote: ... After pre-washing the magnetic beads (50 mM Tris-HCl pH7.5, 500 mM NaCl, 5% glycerol, 0.05% NP-40, 1 mM MgCl2, 0.5 mM PMSF, 1X Sigma protease inhibitor, 10 mM DTT), the Co-IP sample (crude extract + αMyc antibody ...
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bioRxiv - Biophysics 2020Quote: ... 20 µL of 10 % Tween® 20 and 20 µL of a potassium phosphate buffer (4:5 mixture of 1 M monobasic and dibasic potassium phosphate, Sigma Aldrich, USA) were added as well as 10 µL of a 2 nmol thiol-modified single-stranded DNA solution (5’-T20-SH-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... Larvae were then fixed in 1 ml Bouin’s solution (5% acetic acid, 9% formaldehyde, 0.9% picric acid; Sigma Aldrich, St. Lous, MO) for 2 hrs at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mM EDTA, 2.5 mM MgCl2, 25% glycerol, 250 mM sucrose, 5 mM DTT and 1/100X protease inhibitor cocktail, [Millipore-Sigma, Cat# P9599]). After filtration with 2 layers of Miracloth ...
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bioRxiv - Cancer Biology 2021Quote: ... HCECs were grown in a hypoxia chamber with 2% O2 and 5% CO2 at 37°C in 4 parts DMEM to 1 part medium 199 (Sigma-Aldrich #M4530) with 2% cosmic calf serum (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... and proteins were eluted with 4 mL of the same buffer but containing 100 μL (5 mg mL−1) Flag peptide (Sigma-Aldrich F4799). The eluate was spin concentrated (Sigma-Aldrich CLS431485-251A ...
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bioRxiv - Immunology 2021Quote: ... 5% CO2) with 1% BSA RPMI containing 50 ng/ml PMA and 500 ng/ml Ionomycin from Sigma-Aldrich (St. Louis, Missouri); Monensin from BD Biosciences (Franklin Lakes ...
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bioRxiv - Genomics 2019Quote: Neutrophils (1×10^5) were spun onto cover slips using a Cytospin3 (Shandon, 74010121 GB) and flooded with Wright stain (Sigma, WS16-500ML) for 3 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (diluted in 5% BSA in 1X PBS-T) were as follows: Mouse anti-β-Actin (1:10,000, Sigma-Aldrich, A5441), Rat anti-α Tubulin (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 volume of AluI-stock was added to 2 volumes of 5 mg ml−1 fluorescein (FITC) labelled 500 kDa dextran fraction (Sigma-Aldrich, FD500S) dissolved in injection buffer (50 mM K-HEPES ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
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bioRxiv - Biophysics 2021Quote: Mica was freshly cleaved and then pre-treated by depositing 20 μl 0.01% (w/v) 1-5 kDa poly-L-lysine (Sigma-Aldrich, MO, USA), left for 5 mins before washing with 1000 μl filtered milliQ H2O and finally vacuum drying ...
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bioRxiv - Biochemistry 2022Quote: ... The peptides were purified by reverse-phase HPLC (Acetonitrile in Water with 0.1% formic acid) using either a Discovery BIO wide pore C18-5 column (25 cm x 10 mm, Millipore-Sigma #568230-U) or a Shim-pack GIS C18 (20 × 250 mm ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were washed with D-PBS-/- and incubated for 5 min at 37řC with 1 ml of accutase solution (Sigma-Aldrich A6964-500ML). The plate was gently tapped to detach cells and cells were gently pipetted to generate a single cell suspension ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked in 5% BSA in TBST and incubated overnight with primary antibodies targeting the following proteins: FLAG (M2; Sigma; 1:1000), phosphorylated tyrosine (P-Y-1000 MultiMab ...
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bioRxiv - Microbiology 2022Quote: ... M63 borth with 0.2% galactose was made by diluting 500 mL 2× M63 buffer to 1× using 500 mL H2O and adding filter sterilized 0.1 mL 5 mg/mL FeSO4 · 7H2O (Sigma-Aldrich cat. F8633), 1 mL 1 M MgSO4 (Sigma-Aldrich cat ...
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bioRxiv - Microbiology 2022Quote: wtKSHV inoculum was obtained from BCBL-1 cells (5×105 cells/ml) induced with 20 ng of TPA (12-O-tetradecanoylphorbol-13-acetate; Sigma, St.Louis, USA)/ml ...
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bioRxiv - Neuroscience 2022Quote: Mice received an intraplantar injection (unilateral or in both hind paws) of 5 μl λ−carrageenan (primed, 1% w/v, Sigma-Aldrich) to induce transient inflammatory hyperalgesia ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... Cultured astrocytes were treated with 150 pM HIV-1 gp120 CM (ProSpec-Tany TechnoGene Ltd., Israel) for 12 h before application of 5 or 25 μM KYNA (Sigma-Aldrich, USA) for a further 12 h ...
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bioRxiv - Neuroscience 2022Quote: ... −1.8 mm from cortical surface) using glass microcapillary (tip diameter ≃35 μm; 1–5 μL Hirschmann® microcapillary pipette, Z611239, Sigma-Aldrich) connected to a custom Openspritzer pressure system (Forman et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Human AAA specimens were harvested during surgical repair and stored in complete DMEM/F12 Medium containing 5% FBS and 1% PBS (Millipore, Darmstadt, Germany). The tissue was placed in a sterile Petri dish and washed with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Worms were synchronized by bleaching and Day 1 adults were plated on NGM plates containing 100 µM 5-Fluoro-2′-deoxyuridine (FuDR) (Sigma Cat. F0503) with or without 4 mM methyl viologen dichloride hydrate (paraquat ...
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bioRxiv - Neuroscience 2024Quote: ... and probed with the following antibodies (diluted in 5% milk in PBST) overnight at 4 °C: α-Tubulin (1:400000, Sigma t9026), α-Dnmt3a (1:5000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Antibodies were diluted in PBS containing 5% fetal bovine serum (Biological Industries Israel, 04-007-1A) and 1% or 0.1% Triton X-100 (Sigma Israel, X-100), respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... Fluorescein or Texas Red labelled dextran (0.1mL of 1-5% of 70kDa dextran solution in saline; Sigma-Aldrich #46945 or Thermofisher D1830) was injected intravenously immediately before the start of imaging to visualize blood flow ...
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bioRxiv - Microbiology 2022Quote: ... beginning with a virus stock dilution of 1:5 in complete DMEM media as mentioned above supplemented with DEAE-Dextran (Sigma Aldrich #30461) at the final concentration of 40 mg /ml ...
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bioRxiv - Cancer Biology 2023Quote: ... were recovered from Matrigel using 3 mM EDTA in DPBS, washed, centrifuged (200g, 5 min, 4°C) and lysed with 1x RIPA buffer supplemented with PPI (1:100, Sigma- Aldrich, 04693132001), and benzonase (1:100 ...
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bioRxiv - Pathology 2023Quote: ... for 15 min and permeabilized with 1% Triton X-100 (Solorbio, T8200, China) for 15 min and blocked with 5% bovine serum albumin (Sigma, B2064, USA) for 30 min ...