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Citations for Millipore Sigma :
951 - 1000 of 4637 citations for Recombinant Human CEACAM1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... fluorinated oil (Fluorinert FC-40, Cat# 86508-42-1, Sigma-Aldrich) was loaded into the device for sealing microwell arrays after introducing freeze-thaw lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 1% FCS and with 0.1% Penicillin Streptomycin (PS, Sigma), minced and incubated at 37°C with the digestion solution (collagenase 2.5mg/ml in EBSS) ...
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bioRxiv - Microbiology 2020Quote: ... streptomycin (100 mg/ml) and 5% fetal calf serum (FCS) (Sigma). IBDV infections were performed on preconfluent (ca ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 2% (v/v) FCS and 2mM EDTA (Sigma, E7889). Wash buffer consists of PBS supplemented with 2% FCS.
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bioRxiv - Biophysics 2022Quote: ... containing 10mM HEPES (Roth) and 0.5% FCS (Biochrom GmbH, Merck Millipore). One chamber slide was used for gel expansion and the other one as non-expanded control ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in PBS supplemented with 1% FCS (S7524, Sigma) and 2mM EDTA and kept at 4°C at any time ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Media were supplemented with 5% fetal calf serum (FCS) (Sigma-Aldrich) and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with RPMI containing 10% (v/v) FCS (Sigma) and 2 mM L-Glutamine (R10) ...
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bioRxiv - Immunology 2023Quote: ... and 10% (v/v) heat-inactivated fetal calf serum (FCS) (Sigma). Cells were added to sterile 96 well U-bottom tissue culture plates pre-coated with purified NA/LE anti-CD3 (Clone 145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich #A2429), at a 1:4000 dilution was added ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µM ABA or 1 µM Fusicoccin (FC) (all from Sigma) dissolved in absolute ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 20% fetal calf serum (FCS; Sigma-Aldrich cat# F7524) and 50 ng/mL vascular endothelial growth factor (VEGF165 ...
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bioRxiv - Cell Biology 2024Quote: ... was used to prepare unstimulated cells or FCS (Biosera or Sigma) to prepare stimulated cells ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Plant Biology 2019Quote: ... Immuno-blotting was performed using anti-ubiquitin antibody and anti-His (Sigma, USA).
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bioRxiv - Biochemistry 2020Quote: 25 μl of the monoclonal α-His antibody (Catalog-ID H1029, Sigma-Aldrich) were mixed with 100 μl buffer comprising 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: Ezrin constructs in pET-28a(+) (N-terminal hepta-His tag, Novagen, Madison, WI): hEzrin wildtype (His-Ezrin/WT ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Cell Biology 2019Quote: Immunoprecipitations of Flag-tagged proteins were performed using α-Flag M2 affinity beads (Sigma-Aldrich). For each immunoprecipitation reaction ...
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bioRxiv - Cell Biology 2019Quote: ... Washed cells were treated with TRITC tagged Concanavalin A (20 µg ml-1) (Sigma, USA) for 1 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
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bioRxiv - Cancer Biology 2021Quote: ... All FLAG-tagged proteins were purified with anti-FLAG® M2 Affinity Gel (Sigma-Aldrich) in a native condition using the FLAG peptide elution ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma, #A2220). For the IP ...
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bioRxiv - Microbiology 2020Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously (49).
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bioRxiv - Physiology 2021Quote: ... followed by incubation with fluorescent-tagged carboxylate-coated 2 um size latex beads (L3030, Sigma) for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-tagged Pros-S-HD in soluble fraction was purified using Glutathione-Agarose beads (Sigma). The bound proteins were eluted in elution buffer (50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
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bioRxiv - Genetics 2022Quote: V5H6-tagged FRQ was immunoprecipitated with 20 μL of V5 agarose (Sigma-Aldrich, Catalog #7345) from 2 mg of centrifugation-cleared lysate ...
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bioRxiv - Microbiology 2022Quote: ... and FLAG tagged S-protein was stained using anti-FLAG (clone M2, Sigma-Aldrich, F1804). SARS-CoV-2 Spike Antibody (ProSci ...
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bioRxiv - Neuroscience 2023Quote: ... GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich. 200 µg of GST-α synuclein was incubated with the equivalent amount of purified His-spectrin at 4°C overnight in the presence of glutathione sepharose beads ...
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bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...