Labshake search
Citations for Millipore Sigma :
851 - 900 of 4637 citations for Recombinant Human CEACAM1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... These specimens were immersed in Fluorinert (FC-40, Sigma-Aldrich) and allowed to rest for four hours at room temperature before scanning ...
-
bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% FCS (Biowest) and 1% Pen/Strep (Sigma).
-
bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma F-7524), 1000 U/mL LIF ...
-
bioRxiv - Cell Biology 2023Quote: ... The medium was additionally supplemented with 2.5% FCS (Sigma Aldrich) from DIV 9 onwards ...
-
bioRxiv - Immunology 2023Quote: ... and 8% fetal calf serum (FCS, Sigma-Aldrich Chemie BV). TK-1 murine T-cell lymphoma cells (CRL-2396 ...
-
bioRxiv - Immunology 2023Quote: ... complete media supplemented with 10% (vol/vol) FCS (F0804, Sigma), 1% penicillin-streptomycin (15140122 ...
-
bioRxiv - Immunology 2023Quote: ... 70% fetal calf serum (FCS) (Sigma-Aldric, St. Louis, MO) and 20% RPMI 1640 (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... U251 glioblastoma cells were purchased from Millipore Sigma and maintained in DMEM supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma, F7524-500ML) and 1% Penicillin-Streptomycin (Pen/Strep ...
-
bioRxiv - Biophysics 2023Quote: ... which consisted of fluorinated oil (Fluorinert FC-40, Sigma-Aldrich) and 2% w/w fluorosurfactant (RAN biotechnologies ...
-
bioRxiv - Microbiology 2024Quote: ... supplemented with 10% fetal calf serum (FCS) (Sigma Aldrich, F7524) and maintained in a humidified incubator at 37°C and 5% CO2.
-
bioRxiv - Cancer Biology 2024Quote: ... and fetal calf serum (10%, FCS, Sigma-Aldrich-Chemie-BV)) with Geneticin G418 Sulphate (0.6 mg/ml ...
-
bioRxiv - Immunology 2020Quote: ... and primary antibodies in the presence of Fc block in flow buffer (DPBS [Gibco] supplemented with 2% FCS, 2 mM EDTA [both Sigma] and 0.01% sodium azide [Morphisto]). After staining with secondary biotin-coupled antibodies in flow buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... as a His-tag fusion and Ars2206-531 into pRSFduet vector (Novagen) as a Strep-tag fusion ...
-
bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
-
bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...
-
bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Lysis/Binding Buffer (50 mM Na2HPO4 ...
-
bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
-
bioRxiv - Microbiology 2020Quote: ... 10 % heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich), and 1 % antibiotic-antimycotic (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2020Quote: ... The supernatant was loaded onto His-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris–HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... The β-Spectrin-His construct was transformed into BL21(DE3) cells (Novagen) and induced for protein expression with 0.5mM IPTG (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: 30µL/well of rabbit anti-His tag antibody (Sigma Aldrich, catalog #SAB5600227) was adsorbed at a concentration of 0.5 μg/mL on to 384 well microplates (Thermofisher ...
-
bioRxiv - Bioengineering 2022Quote: ... Pre-cleared supernatant was mixed with Ni-NTA His-Bind Resin (Novagen) (Pajenda et al. ...
-
bioRxiv - Immunology 2020Quote: ... supplemented with 15% FBS HI (Life Tehnologies) + 1% Pen/Strept (Sigma-Aldrich), 1% L-glutamine (Life Technologies) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Purification of rcCBP2 was performed by HIS-Select HF Nickel Affinity (Sigma) and gel permeation chromatography on Superdex 75 column (GE Healthcare Life Sciences) ...
-
bioRxiv - Cell Biology 2019Quote: ... for 10 minutes followed by anti-his antibody (Sigma, Cat. No. 11922416001) for another 10 mins ...
-
bioRxiv - Microbiology 2021Quote: ... The crude lysate rocked with HIS-select nickel affinity gel resin (Sigma) for 45 min at 4 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
-
bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-His antibody (Sigma, USA) followed by 1:20000 anti-mouse antibody (Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... The His-tag of the eluted protein was cleaved by thrombin (Sigma), incubated at 4 °C overnight ...
-
bioRxiv - Biophysics 2023Quote: ... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
-
bioRxiv - Plant Biology 2023Quote: ... in-frame of the C-terminal (His)6-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a:ApTPS1 ...
-
bioRxiv - Biophysics 2023Quote: ... then incubated with mouse anti-His tag primary antibody (Millipore Sigma SAB1305538) at 1:1,000 dilution ...
-
bioRxiv - Plant Biology 2024Quote: ... and confirmed by Western blotting using His-tag antibody (Sigma; SAB 1305538). The His-tagged GUN1-PS protein was produced essentially as described (19) ...
-
bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich) at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... and 5% Fetal bovine serum (FBS; Sigma Heat Inactivated, US/HI origin). PBMCs were cultured overnight in chemotaxis media containing RPMI and 1% ultrapure BSA prior to use in assays.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... or human liver microsomes (HLMs) (male human pooled, Sigma-Aldrich), respectively ...
-
bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 proteins were detected using mouse anti-FLAG M2 primary antibody (Sigma), followed by goat anti-mouse Alexa Fluor 568 (Invitrogen) ...
-
Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... the epitope tagged LSF was detected by mouse anti-FLAG antibody (F3165, Sigma-Aldrich) and visualized with an anti-mouse IgG coupled with Alexa Fluor 488 (Molecular Probes) ...
-
bioRxiv - Biochemistry 2020Quote: ... Flag-EGFP tagged ATP11C was eluted with 0.2 mg/ml Flag peptide (Sigma-Aldrich) in the wash buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... and Flag-tagged FtsX proteins were detected by using ANTI-FLAG® antibody (Sigma) as previously described by Stamsås et al ...
-
bioRxiv - Plant Biology 2020Quote: MBP tagged SyYVCV βC1 and mutants were transformed in Rosetta Gammi DE3 cells (Novagen) according to manufacturer’s directions ...
-
bioRxiv - Microbiology 2021Quote: ... the Flag- or HA-tagged proteins were eluted with Flag peptide (Sigma, cat# F4799) or HA peptide (Sigma ...
-
TIR signaling promotes the interactions between EDS1/PAD4 and ADR1-L1 and oligomerization of ADR1-L1bioRxiv - Plant Biology 2021Quote: ... The FLAG-tagged proteins were immunoprecipitated using 20 μl M2 beads (Sigma Cat. #A2220) and biotinylation was detected by Streptavidin-HRP (Abcam Cat ...
-
bioRxiv - Molecular Biology 2021Quote: ... 6xHis-MPB-tagged 43GTPBP6 was expressed in E.coli BL21 (DE3) RIL cells (Merck Millipore) grown in LB media ...
-
bioRxiv - Biophysics 2022Quote: ... Fractions containing tagged ribosomes were concentrated in 100 kDa cut off spin filters (Millipore) and washed with buffer A-1 ...
-
bioRxiv - Microbiology 2023Quote: ... SPA-tagged proteins were detected with monoclonal anti-Flag M2 antibody purchased from Sigma. YbgF protein was used as an internal control and revealed with polyclonal anti-YbgF antibodies ...
-
bioRxiv - Molecular Biology 2023Quote: ... and then with a secondary mouse anti-rabbit IgG tagged with FITC (Millipore, MA). Positively stained areas were quantified and expressed as positive area (μm2)/total area (mm2).
-
bioRxiv - Microbiology 2023Quote: ... and HA-tagged proteases were detected using the anti-HA peroxidase-conjugated antibody (Sigma), according to manufacturers’ instructions.