Labshake search
Citations for Millipore Sigma :
51 - 100 of 7246 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... CUBIC Reagent 1A was prepared using urea (Sigma, 10% (w/w)) ...
-
bioRxiv - Neuroscience 2020Quote: ... Probes were then labelled with DIG or Fluorescein (Roche DIG/Fluorescein RNA Labeling Mix, Sigma Aldrich), tagged with Alexa Fluor 594 or 488 dyes (Invitrogen) ...
-
bioRxiv - Genetics 2022Quote: ... in DIG Easy Hybridization Solution (Sigma) overnight at 45°C and then washed twice each with 2 X SSC ...
-
bioRxiv - Developmental Biology 2023Quote: ... and DIG RNA Labeling Mix (SIGMA) according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2024Quote: ... Anti-DIG-POD (Millipore Sigma #11207733910) was used to detect DIG-labeled probes ...
-
bioRxiv - Biochemistry 2023Quote: SUV420H1 methyltransferase activity was determined by monitoring the production of S-adenosyl homocysteine (SAH) in the presence of S-adenosyl methionine (SAM) (Sigma) and nucleosomes ...
-
bioRxiv - Neuroscience 2021Quote: DIG-labeled DNA probes were synthesized using Roche PCR DIG Probe Synthesis Kit (Millipore Sigma ca#11636090910). Probes were designed to target either the LexA coding sequence (Probe 1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... we synthesized DIG-labeled RNA probes with the DIG RNA labeling mix (Sigma-Aldrich; Cat. No. 11277073910) and T7 RNA polymerase (Promega ...
-
bioRxiv - Evolutionary Biology 2020Quote: All Digoxygenin (DIG) labeled probes were generated using the Roche DIG labeling kit (Sigma Cat no 11277073910). Antisense probes were generated using Pme1 (NEB cat no R0560S ...
-
bioRxiv - Neuroscience 2023Quote: ... DIG epitopes were detected with HRP anti-DIG fab fragments at 1:2500 (Sigma-Aldrich; Reference 11207733910) and revealed using Cy3-tyramide at 1:100 ...
-
bioRxiv - Cancer Biology 2022Quote: ... with collagenase (1mg/ml, Type 1A from clostridium histolyticum, Sigma, cat # C9891) in collagenase buffer (RPMI-1640 supplemented with 2.5 % FBS and 1% HEPES) ...
-
bioRxiv - Biophysics 2020Quote: ... 1A where we used Fluo4-AM (Sigma Aldrich, St. Louis, MO, USA). The commonly used Fluo4-AM is prone to photobleaching ...
-
bioRxiv - Bioengineering 2022Quote: ... with 630 U/mL Type 1A collagenase from clostridium histolyticum (Sigma, #C9891) was added to the tube ...
-
bioRxiv - Genomics 2023Quote: ... supplemented with collagenase type 1A (1 mg/mL, c2674-500 mg, Sigma) and DNase I (10 units/mL ...
-
bioRxiv - Bioengineering 2024Quote: ... with 4.5 ml of HBSS balanced salt solution (Sigma, #02-018-1A) to obtain 5 ml of the coating solution ...
-
bioRxiv - Neuroscience 2020Quote: ... cultures were treated with 1mM Cytosine b-D-arabinofuranoside hydrochloride (Ara-C) (Sigma Aldrich) to inhibit glial cell proliferation ...
-
bioRxiv - Neuroscience 2022Quote: ... cultures were treated with 1 mM Cytosine b-D-arabinofuranoside hydrochloride (Ara-C) (Sigma) at DIV 4 to inhibit glial cell proliferation ...
-
bioRxiv - Developmental Biology 2020Quote: ... DIG-labeled RNA probes were generated using the DIG RNA Labeling Kit SP6/T7 (Sigma-Aldrich cat. # 11175025910) according to manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2023Quote: ... was diluted to 0.01 mg/mL concentration in 100 µL of 25 mM Tris/HCl buffer containing various concentrations of SAHS proteins or BSA (Sigma-Aldrich). 50 µL of each sample was stored at 4°C while the other 50 µL was dried using a Savant SPD131DDA SpeedVac (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... primary mouse anti-DIG (Sigma Roche 11333062910) was added at the dilution of 1 ...
-
bioRxiv - Biochemistry 2022Quote: ... the anti-Dig Fab was from Millipore-Sigma (Burlington ...
-
bioRxiv - Plant Biology 2021Quote: ... 0.1 mM DIG-11-dUTP (Sigma Merck), 0.1 mM dATP ...
-
bioRxiv - Developmental Biology 2024Quote: ... and DIG RNA Labeling Mix (Sigma, 11277073910) to produce probes ...
-
bioRxiv - Molecular Biology 2022Quote: ... at 120,000 microjoules per cm2 and 100 ng/mL of the probe diluted in DIG Easy Hyb Granules Working Buffer was hybridized overnight at 42°C with gentle agitation (Sigma, #11796895001). Next day ...
-
bioRxiv - Genetics 2024Quote: ... Hybridization of the DIG-UTP labeled RNA probe was performed overnight at 65° C and staining was performed using NBT/BCIP (Sigma Aldrich) alkaline phosphatase substrates ...
-
bioRxiv - Plant Biology 2021Quote: ... RNA was crosslinked using UV light and hybridized with a DIG labelled probe (PCR DIG probe synthesis kit, Sigma). For detection of LbuCas13a the membrane was stripped and probed with DIG labelled Cas13a specific probe and signal detected on a Licor Odyssey imaging system (LI-COR Bioscience ...
-
bioRxiv - Molecular Biology 2023Quote: ... sample could be incubated also for 1h with oligo-dT-dig probe (1:1000, polyT-dig Sigma HA09131354-004). Antibodies were diluted 1:500 in blocking buffer ...
-
bioRxiv - Immunology 2023Quote: ... The MLN were minced and digested in supplemented RPMI (Collagenase Type 1A (Sigma), DNAse I 10U/mL (Roche) ...
-
bioRxiv - Plant Biology 2021Quote: ... The protein was eluted with buffer C (buffer B+10%glycerol+2.5 mM Desthiobiotin (Novagen)) in four column volumes ...
-
bioRxiv - Neuroscience 2021Quote: ... The DIG-labeled probes were hybridized to the membrane in Roche DIG Easy Hyb hybridization buffer (Millipore Sigma cat# 11603558001) at 49°C overnight ...
-
bioRxiv - Neuroscience 2022Quote: ... Dig epitopes were detected with alkaline phosphatase-coupled anti-Dig fab fragments antibody at 1:2500 (Millipore Sigma, cat. #11093274910), and the signal revealed with Nitro Blue Tetrazolium chloride/5-Bromo-4-Chloro-3-Indolyl Phosphate ...
-
bioRxiv - Developmental Biology 2021Quote: ... anti-dig alkaline phosphatase conjugate (Sigma Ref. 11093274910), nitro blue tetrazolium (Sigma Ref ...
-
bioRxiv - Developmental Biology 2022Quote: ... and anti-DIG-alkaline phosphatase antibody (Sigma 11093274910) used at 1:5,000 in MAB buffer (100 mM Maleic acid ...
-
bioRxiv - Developmental Biology 2021Quote: ... and anti-DIG-alkaline phosphatase antibody (Sigma 11093274910) used at 1:5,000 in MAB buffer (100 mM Maleic acid ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... for colorimetric visualization or DIG antibody HRP (Sigma) and TSA kit (Perkin Elmer ...
-
bioRxiv - Plant Biology 2021Quote: ... Alkaline phosphatase-conjugated anti-DIG antibodies (Sigma-Aldrich) and NBT/BCIP substrate (Sigma-Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... along with DIG-UTP-labelling mix (Millipore Sigma). Worms harvested from the mesenteries of untreated mice were used to visualize transcripts Smp_076320 ...
-
bioRxiv - Microbiology 2024Quote: ... and DIG RNA labeling mix (Sigma Aldrich, #11277073910) was added as nucleotide mix ...
-
bioRxiv - Developmental Biology 2023Quote: ... and anti-DIG-alkaline phosphatase antibody (Sigma 11093274910) used at 1:5000 in MAB buffer (100 mM Maleic acid ...
-
bioRxiv - Developmental Biology 2023Quote: ... anti-DIG-POD (Roche/Sigma 11207733910, 1:2000) and/or anti-FITC-HRP (Roche/Sigma 11426346910 ...
-
bioRxiv - Microbiology 2020Quote: ... and b (chl b) were identified (RT and maxima absorption spectra) by direct injection of chl a and chl b standards (Sigma-Aldrich C-5753 and 00538, respectively) under the same HPLC conditions ...
-
bioRxiv - Molecular Biology 2021Quote: ... b) PEI only without Bgpiwi siRNAs and c) Mission Universal mock siRNA/PEI complexes (Sigma Millipore). RNA was isolated as described above from washed transfected snails before utilizing for qPCR as described above ...
-
bioRxiv - Molecular Biology 2021Quote: ... b) PEI only without Bgpiwi siRNAs and c) Mission Universal mock siRNA/PEI complexes (Sigma Millipore). RNA was isolated as described above from washed transfected snails before utilizing for qPCR as described above ...
-
bioRxiv - Physiology 2022Quote: The fructose (protocol B) or glucose (protocol C) supplements were provided in powder form (Sigma-Aldrich) that participants dissolved in 1 to 1.5 L of water and drank throughout the day ...
-
bioRxiv - Immunology 2023Quote: ... mouse lung tissues were minced and digested with collagenase 1A (0.5 mg/ml, Sigma) and Dnase I (0.05 mg/ml ...
-
bioRxiv - Genetics 2022Quote: ... Tissue fragments were overnight digested with 1 mg/ml collagenase type 1A (Sigma Aldrich) in culture medium at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... The blot was then neutralized with SSC and hybridized with a DIG-labeled telomere probe prepared as described 48 in DIG Easy Hyb (Sigma-Aldrich) solution overnight at 42°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... Anti-sense DIG-labeled in-situ probes were generated using linearized plasmid cDNA templates with 10X DIG RNA labeling mix (Sigma 11277073910) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... Anti-sense DIG labeled in- situ probes were generated using linearized plasmid cDNA templates with the10X DIG RNA labeling mix (Sigma 11277073910) according to manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2022Quote: ... The membrane was washed and subjected to Anti-DIG-AP antibody by using DIG Wash and Block Buffer Set (Sigma, #11585762001) and immonological detection of the membrane was conducted by CDP-Star chemiluminescent substrate included in the northern blotting kit.