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Citations for Millipore Sigma :
9801 - 9850 of 10000+ citations for Mouse Spermidine Synthase SRM ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies: mouse anti-RSV fusion protein monoclonal antibody (MAB8599, Millipore, USA) or goat anti-human IgG (Licor ...
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bioRxiv - Cancer Biology 2019Quote: ... the TMA slide was subjected to Cell Conditioning 1 (CC1, Ventana) antigen retrieval and stained with a prediluted anti-MCT antibody (Mouse, Clone G3, Millipore #MAB1222) using an automated immunostainer ...
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bioRxiv - Plant Biology 2019Quote: ... 10 µL protein G Dynabeads® were washed twice in ChIP dilution buffer and incubated either with 2 µg of mouse IgG (Sigma), anti-H3K27me3 (Abcam ab6002 ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated 12 min with 5% Triton in PBS and then primary cilia and SMO were stained using a mouse anti-acetylated tubulin Ab (6-11B-1, Sigma-Aldrich) and a rabbit anti-SMO Ab (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... K124 and K42 immunoblots were reprobed with anti-keratin K14 and mouse anti-β-actin-HRP mAb (K124 blots; 1:15,000, clone AC-15; Sigma-Aldrich) or anti-β-actin-HRP alone (K42 blots ...
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bioRxiv - Molecular Biology 2019Quote: ... NF-kB (CST, #3033), PCM1 (CST, #5213) and GABARAP (CST, #26632) and NDP52 (. Secondary antibodies: anti-Mouse IgG–Peroxidase (Sigma, A5906), anti-Rabbit IgG–Peroxidase (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... cell lysates were subjected to immunoprecipitation overnight using an anti-mouse HSF2 (Santa-Cruz) and a non relevant IgG (Sigma-aldrich) as a negative control that were pre-incubated 1h at RT with Protein G UltraLink Resin beads (53132 ...
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bioRxiv - Neuroscience 2019Quote: ... Five 15 cm dishes of 70 – 80 % confluent mouse Schwann cells were separately transfected with 30 μg of pLKO.1-puro vector containing five commercial VDAC1 shRNA (Sigma-Aldrich, sh1#TRCN0000012388 ...
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bioRxiv - Microbiology 2019Quote: ... The plate was washed three times with wash buffer (PBS/0.1% Tween-20) and the primary antibody (anti-NP mouse monoclonal Ab, Millipore, cat. MAB2851) added in blocking buffer ...
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated with HRP-coupled secondary antibodies diluted 1:10,000 in BS (anti-mouse IgG Jackson 515-035-062, anti-rabbit IgG Sigma A-9169), washed twice in BS and twice in TBS ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1% BSA ...
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bioRxiv - Biochemistry 2019Quote: ... rabbit monoclonal DYKDDDDK tag (Flag tag, 1:1000, CST, #14793) and/or mouse monoclonal α-tubulin (1:10,000, Millipore, 05-829). Following three washes with TBS-T ...
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bioRxiv - Microbiology 2020Quote: ... Membrane was further blocked with 5% milk in 1X PBS and Tween 20 for 30 min followed by incubation with mouse anti-gE monoclonal antibody (clone 8612, EMD Millipore) (1:3,000 ...
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bioRxiv - Genetics 2019Quote: ... paraffin-embedded and stained using anti-RAS (G12S) mouse monoclonal antibody (NewEast, 26186) followed by incubation with HRP-conjugated corresponding secondary antibody (Sigma-Aldrich). The expression levels were evaluated by H-score method ...
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bioRxiv - Developmental Biology 2019Quote: Single cell suspensions of wild type AGM and YS at E9.5 and E11.5 were stained with rat anti-mouse CD144-efluor660 1:200 (eBioscience #50-1441-82, clone eBIOBV13) and 7AAD (Sigma, #A9400). Cells were analyzed and sorted on FACSAria (BD) ...
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bioRxiv - Immunology 2021Quote: ... and IP-10) were measured relative to unstimulated cells in supernatants using a Milliplex MAP Magnetic Mouse Cytokine/Chemokine multiplex immunoassay (EMD Millipore).
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bioRxiv - Immunology 2021Quote: ... bound antibodies were detected by addition of a 1 in 5000 dilution of alkaline phosphatase (AP)-conjugated goat anti-mouse IgG (Sigma-Aldrich) for 1 hour at 21°C and addition of p-Nitrophenyl Phosphate ...
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bioRxiv - Microbiology 2021Quote: ... DivK protein separated based on its phosphorylation state was transferred to a PVDF membrane and detected using a chemiluminescent substrate (SuperSignal West PICO PLUS, Thermo Pierce) after incubation with mouse anti-Flag (1:10000) primary antibody (Sigma, F1804) and a polyclonal goat anti-mouse HRP (1:10000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... incubated with secondary anti-Rabbit IgG-Gold coupled antibody (1/10 in BHN1) (Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat (Sigma, G7652), washed twice in BHN2 and HN (50 mM HEPES ...
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bioRxiv - Physiology 2021Quote: ... in maternal serum and placenta was performed with MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assays (MCYTOMAG-70K, Merck Millipore, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour before incubation with primary antibodies: rabbit anti-NogoA (1:200; Supplementary Table 4) and mouse anti-GFAP (1:500; Sigma-Aldrich), for 72 hours at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... They were blocked in 1% BSA in PBT for 1hr at RT and incubated overnight at 4°C with the monoclonal mouse anti-FLAG M2 antibody (Sigma, F1804) diluted 1:500 in 1% BSA/PBS ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitations of 3XF-PPIA and 3XF-Mutant PPIA transiently transfected cells were performed with a mouse monoclonal anti-FLAG antibody (clone M2, Millipore Sigma) in 293T cells ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm) were incubated for 2 h at 30°C with Mouse anti-TH antibody (1:1000, MAB318, Millipore, Burlington, MA) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Neuroscience 2020Quote: ... as well as secondary anti-mouse and anti-rabbit (Thermo Fischer Scientific) Membranes were incubated with Immobilon® ECL Ultra Western HRP Substrate (Millipore) for 3 minutes prior to image acquisition ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with rabbit UPF1 antibody (CST #9435) antibody used at 1:1000 dilution in 5% BSA 1X TBST overnight at 4°C or mouse Tubulin (Sigma T9206) used at 1:2000 dilution in 5% milk 1X TBST for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: Primers were designed to span at least one exon-exon junction and to match the target sequence only in mouse (Primer-Blast, NCBI) and were synthesised commercially (Sigma Aldrich). Primer efficiency was determined separately through a dilution series of cDNA samples from wildtype hippocampus ...
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bioRxiv - Neuroscience 2020Quote: Soluble and insoluble human brain samples and mouse brain samples were homogenized in PBS with a protease inhibitor cocktail (catalog no. 11873580001; Sigma Aldrich) with 3.2 mm stainless steel beads ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslip were washed with PBS with 0.25% triton X-100 and followed by incubation with secondary antibodies Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse (1:1000) together with Hoechst 33342 1 µM (14533; Sigma-Aldrich) for nuclei staining during 1h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... the primary antibody that was used was mouse monoclonal anti-IAV nucleoprotein A1 and A3 blend (EMD Millipore, MAB8251, 1:250). Images were taken at 10x magnification using a Zeiss Axio Observer and Axiocam 503 mono camera (Zeiss).
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bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-GSX2 (1:250 dilution; gift from Kenneth Campbell (Toresson et al., 2000)) mouse anti-Olig2 (1:250 dilution; Millipore (#MABN50), Billerica ...
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bioRxiv - Bioengineering 2021Quote: ... samples were blocked for 1 h in 1% (w/v) bovine serum albumin and incubated with mouse monoclonal anti-vinculin antibody (1:1000, Sigma #V9264) or mouse monoclonal anti-VE-cadherin antibody (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143, Sigma Aldrich, USA; 30 min at 4°C).
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bioRxiv - Biochemistry 2020Quote: SV40-immortalized mouse embryonic fibroblasts (MEF, a kind gift of K. L. Rudolph) were grown in high glucose DMEM medium (Sigma, D6429) until they reached 70 % confluency ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), and rabbit anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2021Quote: ... They were incubated with the following primary antibodies in blocking solution at 4°C overnight: mouse monoclonal anti-GFAP (1:1000, G3893; Sigma-Aldrich), rabbit polyclonal anti-GFAP (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated overnight at 4°C with a tubulin primary antibody (mouse monoclonal anti-β-tubulin III, 1:500, Sigma) and for 2 h with a CY3-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit antibody against Parvalbumin (1:500, PA1-933, Thermo Fischer Scientific) and Mouse antibody against GAD67 (1:500, MAB5406, Sigma-Aldrich) in staining buffer (0.2% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... nonfat dry milk in PBS-T for 1 h Membranes were incubated with mouse anti-FLAG M2 antibody (1:500, Sigma-Aldrich) or mouse anti-α-tubulin B512 antibody (1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were incubated overnight at 4 °C with primary antibodies: mouse monoclonal antibody against α-actinin (1:1,000; Millipore, MAB1682), mouse monoclonal antibody against G6PD (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Neuroscience 2020Quote: ... blocked and permeabilized for 1 h in 10% donkey serum/PBS-T/0.3% triton-X-100 pH 7.4 and incubated for 2 h (RT) in mouse anti-puromycin 12D10 antibody (Sigma Aldrich, MABE343), washed extensively ...
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bioRxiv - Physiology 2021Quote: ... Protein detection was performed by the use of the HRP-conjugated secondary antibodies: anti-rabbit (CST #7074) or anti-mouse (CST #7076) and Immobilon Forte Western HRP substrate (Millipore Sigma). Protein band intensity on exposed film was measured with Adobe Photoshop software.
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bioRxiv - Biochemistry 2021Quote: ... chromatin isolated from 1–2 × 106 cells were incubated with 5 µg of mouse anti-FLAG (F1804, SIGMA-ALDRICH - RRID: AB262044) antibody pre-coupled to Protein A/G Sepharose ...
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bioRxiv - Biochemistry 2021Quote: Anti-FLAG immunoprecipitation was performed using 20 μL of packed agarose-conjugated mouse anti-FLAG antibody (M2 Affinity gel, A2220 Sigma-Aldrich) and were targeted either against the exogenously expressed transgenes (HMR+ ...
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bioRxiv - Biochemistry 2021Quote: ... mouse APC-conjugated antibody directed against dsRNA J2 (IF 1:200, Scicons, 10010500) mouse anti-3xFlag M2 (WB 1:25000, Sigma, F1804), rabbit anti-Tubulin (WB 1:4000 ...
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bioRxiv - Biochemistry 2020Quote: ... Chemiluminescence of the appropriate secondary rabbit or mouse HRP-conjugated antibodies after incubation with ECL HRP substrate (Immobilon™ Western HRP Substrate, Millipore) was detected using a Chemi Doc™ Quantity One® system (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: Free floating mouse brain sections were incubated with 300 nanograms of active human cathepsin-S (SRP0292, Sigma-Aldrich, St. Louis, MO), in activation buffer containing 1.8 mM DTT ...
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins were probed using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, F3165; used at 1:2500) in blocking buffer ...