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Citations for Millipore Sigma :
9751 - 9800 of 10000+ citations for Mouse Spermidine Synthase SRM ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and an Atto 647N protein labeling kit (Sigma-Aldrich, 76508) were used ...
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bioRxiv - Cell Biology 2024Quote: ... organoids were stained with periodic acid-Schiff staining kit (Sigma). LDL uptake was detected using Image-iT™ Low Density Lipoprotein Uptake Kit ...
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bioRxiv - Cell Biology 2024Quote: ... and stained with the Fontana-Masson kit (#HT200-1KT, Sigma), following the manufacturer instructions.
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bioRxiv - Cancer Biology 2024Quote: The QCM 24-well cell migration assay kit (ECM508, Millipore) was used to assess the CXCL10-mediated chemotaxis of OS cells according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the blots were detected using the ECL detection kit (Millipore).
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bioRxiv - Immunology 2024Quote: ... Free Fatty Acid Quantitation Kit was purchased from Sigma-Aldrich.
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bioRxiv - Cell Biology 2024Quote: ... The ChIP assays were performed using EZ ChIP kit (Millipore). The procedure was as described in the kit provided by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... PAS staining was performed using PAS staining kits (Sigma-Aldrich) and ALP activity assay was performed using the Alkaline Phosphatase Staining Kit (Vector Laboratories ...
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bioRxiv - Developmental Biology 2024Quote: ... and the Betafluor Beta Galactosidase Assay Kit (EMD Millipore, 70979), respectively ...
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bioRxiv - Synthetic Biology 2024Quote: ... or plasmids (purified with GenELuteTM Plasmid Miniprep Kit (Sigma-Aldrich) was conducted at at Microsynth AG (Switzerland) ...
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bioRxiv - Neuroscience 2024Quote: ... using the Duolink® Proximity Ligation Assay kit (Sigma-Aldrich). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 100 mg of shoot material was used for RNA extraction using the plant RNA isolation kit Spectrum Plant total RNA Kit (Sigma-Aldrich Chemie GmbH., Steinheim, Germany). The obtained RNA was treated with DNAse (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... the protein was quantified by PierceTM BCA Protein Assay Kit (Thermo ScientificTM, Rockford, IL, USA) and the carbohydrate was quantified by Total Carbohydrate Assay Kit (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Microbiology 2021Quote: ... The concentration of urea in plasma (UreaPLASMA) and PELF (UreaPELF) were determined by using urea test kit (Urea test kit; Sigma Chemical, St Louis, MO, USA) and the absorbance values measured by using spectrophotometer (Wuhan ...
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bioRxiv - Biophysics 2021Quote: ... glass coverslips (Marienfeld, 0117640) were sonicated in 70% ethanol for 5 min and air-dried before coating with mouse laminin (SIGMA L2020) for 1 h at RT and at a final laminin concentration of 25 μg/mL in coating buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... To deplete mouse STK3 expression in HMVP2 cells TRC2 MISSION shRNAs in pLKO.1-puro vector backbone were used (Millipore-Sigma). Lentivirus was generated with psPAX2 and pMD2.G packaging plasmids using with the Polyplus jetPRIME transfection kit in 293T cells ...
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bioRxiv - Cell Biology 2019Quote: ... and immunoblotted with the indicated antibodies followed by HRP-conjugated goat anti–mouse IgG or anti–rabbit IgG or rabbit anti–chicken IgY (Sigma-Aldrich) and Super Signal West Pico Chemiluminescent Substrate (PI34080 ...
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bioRxiv - Cell Biology 2020Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
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bioRxiv - Cell Biology 2020Quote: Cells were stained with rabbit anti-GlActin antibody 28PB+1 (Paredez et al., 2011) and mouse monoclonal anti-HA (Clone HA7, Sigma-Aldrich) both diluted 1:125 in PMEBALG and incubated over-night ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with Alexa-488 goat anti-mouse and Alexa-555 goat anti-α-rabbit (Sigma-Aldrich, St. Louis, MO) (diluted 1:150 in PMEBALG ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with Alexa 488/647-conjugated goat anti-mouse or anti-rabbit (1:100-1:400, Thermo Fischer) and Alexa 488/546-conjugated phalloidin (1:200, Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The membrane was washed four times (each for 10 min) in TBS-T before being incubated with an anti-mouse horse radish peroxidase (HRP)-conjugated secondary antibody (A9044, Sigma-Aldrich) or an anti-rabbit HRP-conjugated secondary antibody (31466 ...
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Developmental Biology 2021Quote: Innervation analysis was done on regenerating limb and flank sections to quantify innervation abundance using the Mouse Monoclonal Anti-Acetylated Tubulin antibody (1:200 dilution – Sigma Aldrich), followed by the Goat-Anti-Mouse IgG Alexa Fluor 488 (1:200 dilution – Abcam ...
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bioRxiv - Developmental Biology 2020Quote: ... a complementary DNA (cDNA) fragment encoding amino acids 448 to 622 of mouse Zcwpw1 was inserted into the p-ET-32a + vector (EMD Millipore) and transfected into BL21-CodonPlus (DE3 ...
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bioRxiv - Microbiology 2021Quote: ... the following were incubated for 15-18hrs at 4°C in PBS with 0.3% Triton-X and 5% goat serum: mouse biotin conjugated anti-NeuN B-clone A60 (MAB377, Millipore, 1:500), rabbit anti-calbindin (C2724 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were then washed and probed with either goat anti-mouse horseradish peroxidase or goat anti-rabbit horseradish peroxidase (Sigma-Aldrich) at a dilution of 1:10000 for 1 hour (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... The conditioned media was filter-sterilized and cytokines and chemokines were measured using Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (MCYTOMAG-70K, Millipore Sigma) and Procartaplex Mouse IFNα/IFNβ Panel (2plex ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by immunocytochemistry with mouse monoclonal anti-vinculin antibody (1:200 dilution in 1% BSA–PBS) (Sigma; V9264, clone hVIN-1) and Alexa 555–labelled goat anti–mouse IgG (#A21422 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated at 37ºC for 1 h with Duolink® in situ PLA® Anti-rabbit PLUS and Anti-mouse MINUS (Sigma) and later washed with 0,01% PBS-tween ...
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bioRxiv - Developmental Biology 2020Quote: ... Zygotes were collected from the oviduct of superovulated females 20h post HCG injection and cultured in EmbryoMax® KSOM Mouse Embryo Media (Millipore) at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then incubated for 1hr with primary antibodies diluted as follows with Block buffer: mouse monoclonal anti-FLAG (Sigma Aldrich) at 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: Tipless silicon nitride AFM cantilevers (NanoWorld, PNP-TR-TL) were coated with monoclonal anti-HA antibodies produced in mouse (H3663, Sigma-Aldrich) or Fc-control for the antibody-based mechano-activation experiments using flexible PEG spacers as described before (Ebner et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: Wild-type E14 mouse ES cells (Sarah Kinkley Lab) were cultured without feeders on gelatin-coated plates (0.1% gelatin, Sigma-Aldrich G1393). The media was changed every day and cells were passaged every 2 days ...
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bioRxiv - Developmental Biology 2022Quote: We developed an injury-mediated mouse model of skeletal muscle regeneration by intramuscular injection of cardiotoxin (CTX) from Naja nigricollis (EMD Millipore) following our established procedure as described earlier [27 ...
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bioRxiv - Neuroscience 2021Quote: ... This was directly followed by incubation overnight at room temperature with primary antibodies (GFAP; host: mouse; diluted 1:1000; Sigma-Aldrich; Cat# MAB360 ...
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bioRxiv - Neuroscience 2021Quote: Mice were sacrificed and the hippocampus of each mouse was dissected and placed in chilled Hanks’ Balanced Salt Solution (Sigma #55021C). The “Frankenstein” protocol [ref] was followed to obtain a nuclei suspension ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody reaction was carried out for 10 days at 4°C in blocking solution (1:1000 mouse monoclonal Tyrosine Hydroxylase antibody; T2928, Sigma-Aldrich). Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... chicken anti-GFAP (Chemicon, AB5541, 1:3000, RRID:AB_177521), mouse anti-SMI-32 (Covance, SMI-32R, 1:2000) and Hoechst 33342 (Sigma Aldrich, 1:300) for nuclear labeling ...
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bioRxiv - Molecular Biology 2021Quote: ... Larvae were blocked in 10% sheep serum in PBDT for 1 hour at room temperature and then incubated in mouse anti-alpha acetylated tubulin (Sigma-Aldrich Canada Ltd ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated overnight at 4 °C in vehicle with primary antibodies (mouse anti-Syt7, 1:200, Neuromab N275/14; rabbit anti-TH, 1:500, Millipore AB152). After 3 washes for 30 minutes in vehicle ...
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bioRxiv - Neuroscience 2021Quote: ... washed as above described and re-blotted using mouse anti-β-actin antibody (Sigma-Aldrich, USA; 1:2500, 1 h, RT). Again ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated in blocking solution [PBS with 3% bovine serum albumin prior to the addition of a primary mouse monoclonal antibody specific for phospho-Histone H2AX (Ser13; clone JBW301; Millipore Sigma). Following incubation ...
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bioRxiv - Cell Biology 2022Quote: ... followed by probing with horse radish peroxidase (HRP)-conjugated α-mouse (1:10000) (Santa-Cruz Biotech) and detection of signal after chemiluminescent HRP substrate (Millipore) treatment.
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bioRxiv - Cell Biology 2022Quote: ... The cDNA encoding mouse BANF1/BAF was synthesized using two120-mers and a 90-mer single-stranded DNA oligonucleotides (Table 2, Sigma-Aldrich). These ssDNA oligonucleotides each contain a 15-mer overlapping sequence at both ends ...
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bioRxiv - Microbiology 2019Quote: Mouse hnRNPC (EMU173751)-targeting Endonuclease prepared short interfering RNA (esiRNA) and scrambled esiRNA as negative-control were purchased from Sigma-Aldrich. Neuro2A cells upon achieving 60% confluency were undergone transfection with 20 Pico mole of hnRNPC esiRNA along with lipofectamine RNAiMax (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... anti-GFP (in house made rabbit polyclonal antibody; 1:1,000-4,000) anti-Vinculin (mouse monoclonal; clone hVIN-1; Sigma-Aldrich; 1:10000), anti PMF1/NNF1 (in house made mouse affinity purified monoclonal ...
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bioRxiv - Immunology 2019Quote: ... cells were washed twice with PBS for 1 h at 37°C and then incubated with a FITC-conjugated rabbit anti-mouse IgG antibody (Sigma-Aldrich) at a 1:500 dilution ...