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Citations for Millipore Sigma :
9601 - 9650 of 10000+ citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (anti-vGluT1, 1:1000, guinea pig, Millipore and anti-MAP2, 1:1000, rabbit, Millipore). Sections were washed 3 times for 10 mins each in 1x PBS ...
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bioRxiv - Genetics 2022Quote: ... The slices then incubated with primary antibodies with denoted dilutions overnight at 4°C (anti-Iba1 goat polyclonal, Abcam ab5076, 1:200; anti-HNA mouse monoclonal, Millipore MAB1281 ...
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bioRxiv - Cancer Biology 2022Quote: ... Horseradish peroxidase conjugated secondary antibodies were added to the membranes (anti-rabbit, Invitrogen; 31460 and anti-mouse EMD Millipore; AP308P). Membranes were developed with ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse IgG (H+L) HRP conjugated (Chemicon International AP308P) and Anti-Rabbit IgG (whole molecule)–Peroxidase (Sigma Aldrich A9169). Chemiluminescence detection was done with SuperSignal West Pico or Femto (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Anti-FLAG tag or anti-mCherry primary antibodies were used at a concentration of 1:1,250 (Sigma, St. Louis, MO). Then ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking in 5% BSA, membranes were incubated with Anti-FASN rabbit antibody (#3189, CST) antibody or mouse anti-tubulin antibody (Sigma). Protein was next incubated with HRP-conjugated secondary antibodies and was detected using chemiluminescence.
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bioRxiv - Microbiology 2019Quote: ... mouse anti-rabbit IgG antibody:alkaline phosphatase (Sigma-Aldrich; anti-mouse IgG (whole molecule:alkaline phosphatase antibody produced in rabbit (Sigma-Aldrich), and anti-rabbit IgG ...
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bioRxiv - Zoology 2019Quote: ... and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin, Sigma T9028 and rabbit anti serotonin ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunoprecipitation of protein extracts was performed using a monoclonal anti-Flag antibody covalently attached to agarose (Anti-FLAG M2 Affinity gel, Sigma). Proteins were eluted in 2xNuPAGE LDS Sample Buffer (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... the membranes were incubated with the appropriate horseradish-peroxidase conjugated secondary Ab (1:5000 in TBST + 2% BSA; 2 h at room temperature; anti-mouse A4416 and anti-rabbit A0545, Sigma). The immunoreactivity was detected by ECL reagents (Amersham) ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse monoclonal anti-Tau-1 (05-838, 1:1000), mouse monoclonal anti-MAP2 (05-346, 1:500), rat monoclonal anti-tubulin (MAB1864, 1:500) from Millipore; chicken polyclonal anti-GFP (ab13970 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-synaptophysin (1:500; SMC-178D, StressMarq Bio.) and rabbit monoclonal anti-GluR1 (1:500; 04-855, Millipore). Secondary antibodies were Alexa Fluor 488-donkey anti-mouse IgG (1:300 ...
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bioRxiv - Cell Biology 2020Quote: ... washed three times with TBST and incubated with the primary antibody – the polyclonal anti-Dam1p (Keating et al., 2009) or monoclonal anti-GFP antibody (#11814460001 from Sigma) overnight at 4 °C ...
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bioRxiv - Physiology 2020Quote: ... the membrane was incubated with rabbit anti-Urn8R (1:1000) and mouse anti-tubulin (1:2500; Sigma-Alrich, MO, USA) in blocking buffer supplemented with 0.2% Tween 20 (w/v) ...
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bioRxiv - Biochemistry 2021Quote: ... and the secondary fluorescence-labeled anti-mouse IgG antibody (2.5 µg/mL; goat anti-mouse IgG, Atto647N, Sigma Aldrich, Germany) were mixed in one batch and offered in a volume of 2 µL to the beads from the opposite side ...
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bioRxiv - Plant Biology 2021Quote: ... GSTU5 was detected by the anti-GST primary antibody (Novex, 1:500) and anti-mouse secondary antibody (Sigma, 1:5000).
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Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threatsbioRxiv - Molecular Biology 2019Quote: ... Tubulin as loading control was detected with anti-tubulin (1:10000, MBL) and HRP-conjugated anti-rabbit IgG as the secondary antibody (1:10000, Sigma). For detecting the heperphosphorylation of Crt1-13MYC ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatant was incubated with 100μl Anti-HA Affinity gel (D111140, Sangon Biotech, China) or Anti-Flag M2 Affinity Gel (A2220, Sigma, USA). Incubation lasted overnight at 4℃ with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse monoclonal anti-Na+/K+ ATPase (1:500; SantaCruz) and anti-acetylated tubulin (1:2,000; clone 6-11B-1, Sigma Aldrich). Goat anti-mouse or rabbit IgG conjugated with HRP were used for secondary antibodies (1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 h at 4 βC with primary antibodies (rat anti-BrdU, 1:500, OBT Serotec; mouse anti-NeuN, 1:200, MAB377, Millipore; rabbit anti-S100β ...
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bioRxiv - Developmental Biology 2021Quote: ... Antibodies used were chicken anti-GFP (1:10,000, Aves GFP-1010) and mouse anti-β-tubulin (1:5000, Sigma T4026).
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bioRxiv - Cancer Biology 2020Quote: ... serum samples used to detect levels of IgG by anti-IgG-HRP antibody (Bio-Techne) or IgM by anti-IgM-HRP antibody (Sigma) on 100μg/ml NP-KLH-coated 57 ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with primary antibodies (anti-Vimentin, 1:200 dilution, Abcam; anti-cytokeratin 14, 1:200 dilution, Millipore, USA ...
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Biophysics 2020Quote: ... Polyclonal rabbit anti-clathrin heavy chain (Cat. Nr. SAB1410212) and rat monoclonal anti-β1-integrin (Cat. Nr. MAB1997) were from Sigma. Rabbit monoclonal anti-caveolin-1 (Cat ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysates were then subjected to EZview Red Anti-HA Affinity Gel or Anti-FLAG M2 Affinity Gel (Sigma-Aldrich), incubated 3 h on rotation at +4 °C and washed three times with IP buffer without Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... 15 µl of secondary antibody (anti human Fab-HRP: Jackson ImmunoResearch, cat# 109-035-097; anti human IgG-HRP: Sigma, cat# A6029-1ML ...
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bioRxiv - Immunology 2020Quote: ... then binding was detected with specific alkaline phosphatase-conjugated secondary antibodies (anti-mouse total IgG and anti-monkey total IgG from Sigma and anti-mouse IgG1 and IgG2a from BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... C1q fixation was detected with rabbit anti-C1q antibodies (1/2000; in-house) and a goat anti-rabbit-HRP (1/2500; Millipore). TMB liquid substrate (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: The following primary antibodies were used for immunoprecipitation assay and/or immunoblotting: a mouse anti-Flag and a mouse anti-Flag-HRP antibody (Sigma), a rabbit anti-N antiserum (70) ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated with rabbit anti-TMEM43 polyclonal antibody (1:100, NBP1-84132, Novus) (Ab1-2) and mouse anti-FLAG (1:500, F1804, Sigma) for overnight ...
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bioRxiv - Immunology 2021Quote: ... mouse monoclonal anti Rho-GDI (Santa-Cruz Biotechnology, 1:500) and mouse monoclonal anti-FLAG M2 Ab (Sigma, 1:1000), anti-ERK1/2 (Cell Signaling Technology ...
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bioRxiv - Genetics 2020Quote: ... 4 RIMBP1 WT and 4 RIMBP1 KO cerebelli were cut parasagittal (30 um) and stained with antibodies anti-GAD65 (1:1000, mouse, DSHB) and anti-calbindin (1:1000, rabbit, Millipore). Synaptic puncta were imaged using a Nikon confocal microscope (A1RSi+ ...
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bioRxiv - Microbiology 2022Quote: ... These were prepared by preincubating the His-tagged PilC with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 1:1.5:1.5 (by weight ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586; anti-N2D, Millipore, MAB5578 ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunoprecipitates were washed with RIPA buffer and analysed by Western blot using anti-Kkv or anti-Reb antibodies and the Immobilon ECL reagent (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... The proteins were separated on SDS-PAGE gels and analyzed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10; anti-FLAG M2, 1:1,000 dilution, Sigma, F1804).
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti- FLAG (catalog #: F1804) and anti-β-actin (catalog #: A1978) antibodies came from Sigma (St. Louis, MO). The mouse monoclonal anti-Hsp47 antibody (catalog # ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... RPN11- 3xFLAG tagged subunits were detected by monoclonal anti-FLAG antibodies (Monoclonal ANTI- FLAG® M2 antibody #F3165, Millipore Sigma). The lysate from a wild-type strain with untagged RPN11 was used as a negative control ...
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bioRxiv - Molecular Biology 2022Quote: ... PLG binding was detected by adding an anti-PLG primary antibody raised in sheep (Acris Antibodies) followed by horseradish (HRP)-conjugated anti-sheep secondary antibody (Sigma). Primary and secondary antibodies were diluted 1:1000 and 1:2000 ...
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bioRxiv - Molecular Biology 2022Quote: ... the wells were incubated with an anti-PLG primary antibody raised in sheep (Acris Antibodies) followed by horseradish (HRP)-conjugated anti-sheep secondary antibody (Sigma). Primary and secondary antibodies were diluted 1:2000 and 1:4000 ...
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bioRxiv - Pathology 2022Quote: ... The membranes were washed three times with Tris-buffered saline and Tween-20 followed by incubation with the peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (both from Millipore) for 1 h at room temperature.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Secondary antibody HRP conjugated-rabbit anti-goat IgG (cat#: AD106P) and primary anti-MPO antibody (cat#: AF3667) were purchased from Sigma and R&D systems ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were washed with TBS-T and membranes were incubated with secondary antibodies anti-mouse and anti-rabbit coupled to alkaline phosphatase (1:5000; Sigma) diluted in TBS-T/1% BSA for one hour at RT ...
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bioRxiv - Cell Biology 2024Quote: ... anti-vinculin (Cat#V9131, 1:30K), and anti-PMP70 (Cat#SAB4200181 for WB, 1:1000) antibodies were obtained from Sigma. Anti-p62 (Cat#sc-28359 ...
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bioRxiv - Microbiology 2022Quote: ... the microarrays were overlaid with the VP1 proteins for 90 minutes as protein-antibody complexes that were prepared by preincubating VP1 with mouse monoclonal anti-polyhistidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 4:2:1 (by weight ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were incubated in primary antibodies (Anti-LAMP1 1:100 Invitrogen CAT#14-1079-80; anti-Iba-1 1:250 Abcam CAT#ab5076; anti-dsDNA 1:250 Millipore CAT#MAB1293 ...
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bioRxiv - Cell Biology 2022Quote: ... Dylight 550-labeled rabbit anti-BUB-1 (custom made, 74) and FITC-labeled mouse anti-α-tubulin (SIGMA F2168, DM1α) were used at a concentration of 1 μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated with mouse primary anti-Collagen Type II (DSHB II-II6B3) or mouse anti-dopamine 4 receptor (MABN125, Millipore) primary for 2 h at room temperature in a humidified chamber ...
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bioRxiv - Bioengineering 2024Quote: ... MyoD (primary Anti-MyoD Antibody, #ZRB1452, Sigma-Aldrich, and an anti-rabbit secondary antibody coupled with Rhodamine #SAB3700846, Sigma-Aldrich), DAPI (4′-6-diamidino-2-phenylindol ...