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Citations for Millipore Sigma :
9501 - 9550 of 10000+ citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Proteins were detected using either secondary anti-mouse or anti-rabbit IgG coupled to peroxidase (Millipore, 1:5000 dilution), western bright ECL substrate (Advansta ...
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bioRxiv - Physiology 2022Quote: ... onto PVDF membrane then blotted overnight at 4°C with anti-RBM20 antibody (Myomedix) or anti-γ-tubulin (Sigma) antibodies ...
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bioRxiv - Microbiology 2024Quote: ... Horseradish peroxidase-conjugated rabbit anti-rat IgG and horseradish peroxidase-conjugated goat anti-chicken IgY (Sigma, St. Louis, MO) were used for western blotting at a dilution of 1:80,000 ...
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bioRxiv - Cell Biology 2022Quote: ... used for HA::SYP-1) or mouse anti-HA (SYP-4) and mouse anti-α-tubulin (DM1A, EMD Millipore), each diluted 1:5,000 ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were rabbit anti-MAP2 (ab281588, abcom) and mouse anti-β-tubulin III (T8578, Sigma-Aldrich). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... #9664, CST), rabbit anti-GAPDH (1:10,000, #2118, CST) and mouse anti-β-actin (1:10,000, #A5441, Sigma-Aldrich). GAPDH and β-actin served as loading controls.
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies include: anti-V5 Tag (Invitrogen, ref R96025, 1:500, anti-HA (Sigma-Aldrich, ref H6908, 1:500), anti-Akt (pan ...
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bioRxiv - Microbiology 2024Quote: To assess localisation immunofluorescence assays were performed as described previously (31) using anti-HA (1:500, anti-rat,Sigma), anti-MYS (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... Mouse monoclonal anti-FLAG M2 antibody (F1804) and mouse monoclonal anti-β-actin antibody (A1978) were obtained from Sigma. HRP-conjugated goat anti-rabbit (CST#7074 ...
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bioRxiv - Plant Biology 2024Quote: ... anti-FLAG (for both the SUVH1-3xFLAG and the DMS3-ZF ChIPs, we used Anti-FLAG M2 (Sigma, F1804)) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA tag (CST, 2367, 1:1000 for WB) and anti-β-actin (Sigma, 2228, 1:2000 for WB) antibodies followed by incubation with HRP-conjugated secondary antibodies (CST ...
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bioRxiv - Immunology 2023Quote: ... mouse anti-β-tubulin (#T8328) and rabbit anti-phospho-TDP-43 (#SAB4200223) from Sigma Aldrich (St. Louis, MO, USA). Secondary antibodies labelled with horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were prepared in 3% bovine serum albumin 1X TBS-T with 0.02% sodium azide to the appropriate dilution: anti-Flag and anti-HA (Sigma) 1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-phospho-ERM (1/5000) (Roubinet et al., 2011) and rabbit anti-actin (Sigma-Aldrich A5060, 1/10,000) were also used ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was immunoprecipitated with anti-PPARγ (A3409A, Perseus Proteomics, Tokyo, Japan) or anti-mouse IgG (Millipore, 12-371) antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Rat anti-Ago2 (cat no. SAB4200085) and goat anti-Apolipoprotein B (cat no. AB742) were purchased from Millipore Sigma. Rabbit anti-CD63 (cat no ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by probing with secondary HRP conjugated anti-mouse or anti-rabbit antibody (1:2000 dilution, SIGMA). Proteins were visualized using the Clarity Western ECL substrate (Bio-Rad 1705060EM ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated for 1 h at RT with secondary AP-conjugated anti-mouse or anti-rabbit antibodies (1:20,000, Sigma), washed three times for 5 min with PBT and once for 15 min in 20 mM Tris-HCl (pH 9.8 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 % [v/v] Triton-X-100) and incubated with anti-HA or anti-FLAG M2 resin (Millipore-Sigma, USA) for 1 h at 4°C for immunoprecipitation of protected fragments.
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-SUN1 pAb (HPA008346) and mouse anti-β-tubulin mAb (T4026, clone TUB2.1) were also from Sigma-Aldrich. Both of them were used at a dilution of 1:200 for immunofluorescent microscopy and at a dilution of 1:1000∼2000 for western blotting ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-Osk (1:1000) (kind gift from Dr. Paul Lasko, McGill University) and rabbit anti-GFP (1:1,000) (Millipore).
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bioRxiv - Neuroscience 2023Quote: ... followed by overnight incubation at 4°C with primary antibody diluted in block (Rb anti-TMEM106B, 1:1,000, Sigma HPA058342 and Ck anti-GAPDH, 1:10,000, Millipore ab2303). The following day ...
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bioRxiv - Biochemistry 2023Quote: ... followed by primary antibody incubation in PBS+ for 1.5 hr at room temperature (1:15,000 Rb-anti-RAD51 (#70-001, BioAcademia, Japan) and 1:5,000 M-anti-γH2AX (#05-636, Millipore, USA)) ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM NaCl) and subsequent incubation with 300 nM anti-FLAG antibody (monoclonal ANTI-FLAG® M2 from Sigma) for 120 min ...
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bioRxiv - Bioengineering 2023Quote: ... Detection of IgG1 employed anti-human IgG (VH+VL) (Jackson) and of IgA2 anti-human IgA (Hc only) (Sigma). Commercial antibodies were used at dilutions recommended by manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 % [v/v] Triton-X-100) and incubated with anti-HA or anti-FLAG M2 resin (Millipore-Sigma, USA) for 1 h at 4°C for immunoprecipitation of protected fragments.
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bioRxiv - Neuroscience 2023Quote: ... rabbit polyclonal anti-Laminin (1:50; Santa Cruzcat # sc20142) and mouse monoclonal anti-CSPG (1:200; Sigma cat # C8035). Phalloidin Alexa Fluor 594–conjugated (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 1:500 dilution of rabbit anti-MeCP2 (Cell signalling) and 1:10000 dilution of mouse anti-vinculin (Sigma Aldrich). After the incubation with the primary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse and anti-rabbit IgG horseradish peroxidase (HRP)-linked antibodies (Amersham) and streptavidin HRP-conjugated antibodies (Sigma-Aldrich) were used for ECL detection Reagents (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... the bound antibody was incubated with horseradish-peroxidase labelled subclass-specific anti-mouse IgG1 at 1:8,000 or anti-mouse IgG2c at 1:2,000 (Sigma, Aldrich). After five washes with PBS-T ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibodies (anti-TDP-43 1:750, PTGLabs; anti-SUMO2/3 [8A2] 1:500 Sigma) diluted in blocking buffer (1.5% Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: ... Rabbit polyclonal anti-PEX14 was obtained from ProteinTech (cat. 10594-1-AP). Rabbit anti-calnexin (cat. C4731) and rabbit anti-TGN46 (cat. T7576) were from Sigma-Aldrich. Mouse anti-p230 was from BD Bioscience (cat ...
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bioRxiv - Cell Biology 2024Quote: ... anti-N protein (1:1000, CiteAb #40588-T62) and mouse anti-acetylated α-tubulin (1:200, Sigma-Aldrich #T7451). The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed thrice with TBS-T buffer prior to addition of horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in blocking solution (anti-mouse-HRP 1:10,000 or anti-rabbit-HRP 1:10,000 antibodies; all from Sigma Aldrich) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubating with the secondary antibodies (anti-Mouse MINUS, DU092004, Sigma-Aldrich; anti-Rabbit PLUS, DUO92002, Sigma-Aldrich) for 1 h at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... 1:200 dilution,36 anti-Yb (1G2H3, 1:200 dilution),56 anti-Flag M2 (F3165, 1:1,000 dilution, Sigma) monoclonal and anti-c-Myc (1:300 dilution ...
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bioRxiv - Bioengineering 2024Quote: ... the medium was changed to full DMEM (10% FBS and 1% Anti-anti) with 10mM sodium butyrate (Sigma-Aldrich) to boost CMV promoter activity ...
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bioRxiv - Neuroscience 2024Quote: Biotinylated proteins were eluted from streptavidin beads by the addition of 20 μL of elution buffer (2X Laemmli sample buffer [Bio-Rad], 20 mM dithiothreitol [Sigma-Aldrich], and 2 mM biotin [Sigma-Aldrich]) followed by a 10 min incubation at 95°C ...
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bioRxiv - Neuroscience 2021Quote: ... sections were stained with primary (1:1000, guinea pig anti-NeuN, Millipore #ABN90P; 1:1000, mouse anti-NeuN, Millipore #MAB377) and secondary antibodies (1:400 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were cleared by centrifugation at 12000 rpm for 10 minutes and incubated with anti-flag (anti-flag M2 Affinity Gel, Sigma) antibodies covalently attached to agarose 4 hours at 4 °C on a rocking platform ...
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bioRxiv - Cancer Biology 2021Quote: Anti-Flag (Cat. number: F7425) and anti-His (Cat. number: SAB1306085) antibodies were from Sigma-Aldrich (St. Louis, MS, USA). Anti-phospho-γ-H2AX (Ser139 ...
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bioRxiv - Cell Biology 2020Quote: ... For visualization of blue protein bands from the prestained protein ladder the anti-BLUE antibody (Anti-BLUE, 2D2-F11, Millipore) was used (Schuchner et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified lysates were incubated with equilibrated anti-Flag M2 affinity gel (ANTI-FLAG M2 Affinity Gel Sigma-Aldrich, A2220) on a rotating wheel for 3 h at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... The protein lysate was then separated by SDS-PAGE and blotted with anti-HA (Cat# HT301-02, TransGen Biotech, Beijing) and anti-FLAG (Cat# F1804, Sigma) primary antibodies and HRP-conjugated secondary antibodies (Cat# A9044 ...
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bioRxiv - Microbiology 2020Quote: ... the samples were probed with rabbit polyclonal anti-Spo0A or anti-FliC antibodies at a 1:1000 dilution and an anti-rabbit secondary antibody conjugated to horseradish peroxidase (Sigma) was used at dilution 1:10000 ...
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bioRxiv - Developmental Biology 2021Quote: Immunocytochemistry was carried out on fixed unpermeabilized cells with polyclonal antibodies anti-Xlenpp4 used at 1/200 or anti-mEnpp4 (CR65; see31) used at 1/400 and anti-rabbit IgG FITC (Sigma) at 1/80 ...
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bioRxiv - Molecular Biology 2019Quote: ... Myofibers present in a muscle section were stained with an anti-myosin antibody (MF 20, DSHB) while anti-laminin antibody (Sigma) was used to visualize the outline of all myofibers present in a given muscle section ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then fixed and rat anti-MBP (1/5) [11] and rabbit anti-NF-H (1/1000, EMD Millipore) primary antibodies were added o/n at 4°C ...