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Citations for Millipore Sigma :
9451 - 9500 of 10000+ citations for Rabbit Anti Human IgG+IgM+IgA Alexa Fluor 660 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2019Quote: ... sections were incubated with c-myc antibody (1:1000, rabbit polyclonal, Sigma-Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... Cy3 (Indocarbcyanine) conjugated mouse or rabbit secondary antibodies were purchased from EMD Millipore and Alexa633- / Alexa488-conjugated secondary antibodies were procured from Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... USA) and the α-Muc5B rabbit polyclonal antibody (HPA008246) was from Sigma-Aldrich. Fluorescently labeled secondary antibodies were obtained from Molecular Probes (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... which included 6% rabbit serum (complete media from Sigma-Aldrich, St. Louis, Missouri) at 33°C.
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bioRxiv - Neuroscience 2019Quote: The following antibodies were purchased: CNTNAP2 (C-terminal, rabbit polyclonal, Millipore, Darmstadt, Germany), CNTNAP2 (N-terminal ...
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bioRxiv - Microbiology 2020Quote: ... A 1:2500 dilution of alkaline phosphatase conjugated α-rabbit secondary antibody (Sigma) in TBS-T 0.1% supplemented with 0.1% BSA was applied and blots incubated for 2 hours at room temperature followed by the washing procedure described above with the addition of a final 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... We used a rabbit polyclonal antibody (1:1000, Millipore/Upstate, Cat. #07–432) to label KCC2 ...
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bioRxiv - Cell Biology 2022Quote: ... using the Duolink In Situ Red Starter Kit Mouse/Rabbit (#DUO92101, Sigma-Aldrich), according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Immunology 2022Quote: ... and Cy3 (Indocarbocyanine) conjugated mouse or rabbit secondary antibodies were purchased from Millipore. N-ethylmaleimide (cat no ...
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bioRxiv - Genomics 2022Quote: ... using 20 μg CTCF antibody (rabbit polyclonal, Merck Millipore 07-729, lot 2517762). Library preparation was performed using immunoprecipitated DNA or input DNA (max 50 ng ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit polyclonal antibody specific for the C terminus of Aβ40 or Aβ42 (Millipore) was coated onto plates ...
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bioRxiv - Cell Biology 2022Quote: ... and non-muscle myosin II produced in rabbit (1:100, Sigma-Aldrich M8064). In some samples ...
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bioRxiv - Cell Biology 2023Quote: ... The Duolink® In Situ Orange Starter Kit mouse/rabbit (Sigma Aldrich, USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: rabbit antimCherry (1:500, Millipore, Abcam, ab167453), mouse anti-PV (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: Rabbit polyclonal antibody to AQP4 (cat. HPA014784) was from Millipore Sigma (Rockville, MD). Mouse monoclonal antibody to VE-cadherin (cat ...
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bioRxiv - Neuroscience 2023Quote: We used primary antibodies against Purkinje cell marker calbindin (rabbit, Sigma-Aldrich, C9848) as previously described (Asher et al. ...
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bioRxiv - Genetics 2024Quote: ... and incubated with 50 uL rabbit complement serum (1: 10, Cat#S7764, Sigma) at room temperature for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices were then incubated with 1:500 rabbit-↵-TH (MAB318, Sigma Aldrich) and 1:500 mouse-↵-GFP (SAB2702197 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The primary and secondary antibodies were 1:2000 rabbit α-GAPDH (Sigma-Aldrich)/3% BSA/TBSA and 1:20,000 HRP-conjugated goat α-rabbit IgG (Sigma-Aldrich)/1% BSA/TBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunocomplexes were detected with horseradish peroxidase-conjugated rabbit or mouse secondary antibodies (Sigma) followed by enhanced chemiluminescence reaction (Millipore ...
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bioRxiv - Genomics 2024Quote: ... was washed with ChIP dilution buffer and then incubated with around 1.5 μg antibodies (anti-H3K4me3, Millipore-Sigma, 07–473; anti-H3K36me3, Abcam, ab9050; anti-H3K27me3, Millipore-Sigma ...
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bioRxiv - Genomics 2024Quote: ... was washed with ChIP dilution buffer and then incubated with around 1.5 μg antibodies (anti-H3K4me3, Millipore-Sigma, 07–473; anti-H3K36me3, Abcam, ab9050; anti-H3K27me3, Millipore-Sigma, 07–449 ...
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bioRxiv - Biochemistry 2023Quote: ... 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000, anti-UBR4/p600 ab86738, Abcam, 1:5000, anti-FLAG M2, Sigma F1804 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated in primary antibody (Anti S100A9, 1:200, CST; Anti Iba-1, 1:200, Wako; Anti NeuN, 1:200, Millipore; Anti NF-κB p65, 1:200, CST) at 4 °C overnight and in secondary antibody (Goat Anti-rabbit 1:200 ...
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bioRxiv - Microbiology 2021Quote: ... as secondary antibody, A purified mouse IgG (D50, NIH AIDS Reagent Program) as standard and o-phenylenediamine dihydrochloride (Sigma-Aldrich, #P8787-100TAB) as substrate ...
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bioRxiv - Cell Biology 2023Quote: ... 137 mM NaCl2) before blocking for 1 hour at RT with blocking solution (2% BSA IgG free and protease free (Sigma-Aldrich, A3059); 0.1 % Tween 20 (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... Mock RNA co-IP controls were prepared in an identical manner using either naïve polyclonal mouse IgG (control for α-ORF1p; Millipore Sigma, #I5381) or naïve polyclonal rabbit IgG (control for α-ORF2p ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in RPMI-1640 complemented with 10% IgG-free Fetal Calf Serum (Sigma-Aldrich, #F1283) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were incubated with secondary antibody conjugated with 2 µg/ml Alexa dye (Thermo) and 300 nM DAPI (Sigma) in blocking solution for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... cells and sections were subsequently incubated with Alexa Flour 488/594/647-conjugated secondary antibodies and counterstained with DAPI (1:5000; Sigma). Immunofluorescence staining was examined using a confocal microscope.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with PBS and incubated with EdU click-It reaction (HEPES pH 7.5 125mM, CuSO4·5H2O 20mM, Ascorbate 100mM, Alexa Azide 647 (Sigma, A10277) 5mM) ...
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bioRxiv - Biochemistry 2022Quote: ... Alexa FluorTM 555 NHS stock solution was prepared immediately before use by solubilizing the dye in DMSO (Sigma-Aldrich, >99.9 %) to stock concentration of 1.6 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... Reaction mixes were made with 10 μM bovine tubulin (PurSolutions) containing 5% Alexa 568-labeled bovine tubulin and 1 mg/mL BSA (Sigma) in assay buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1143-1300 of human RTEL1 were amplified by PCR then subcloned between the EcoRI and XhoI restriction sites of pET28 (Novagen). RTEL1 proteins were expressed as 6-histidine-fusions in the E ...
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bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
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bioRxiv - Cell Biology 2019Quote: ... S7077) and used at a final concentration of 10 µM. Human recombinant HGF (Cat. Nr. 1404) was purchased from Sigma and used at a final concentration of 100 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 (human epithelial colorectal adenocarcinoma cell line) cells were purchased from ATCC and cultured in DMEM high glucose media (Sigma). The cells were trypsinized using 0.25% trypsin-EDTA and resuspended in the fresh media ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: The cDNA residues 1-169 of human Rheb (184 amino acid isoform, GenBank accession number EAW53989) was subcloned into the pET28a vector (Novagen), with an N-terminal 6×His-tag ...
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bioRxiv - Pathology 2022Quote: ... biopsies of human xenograft harvested at the indicated time post infection were digested with 0.4 mg/ml Liberase TL (Sigma Aldrich) in CO2-independent medium for 60 min at 37°C with gentle agitation (100 rpm) ...
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bioRxiv - Immunology 2019Quote: The sequence-encoding residues Val77–Leu233 of human TNFα was cloned and fused to the N-terminal 6xHis tag in pET-28a+ vector (Novagen) and transformed into Escherichia coli Rosetta (DE3 ...
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ArchR: An integrative and scalable software package for single-cell chromatin accessibility analysisbioRxiv - Genomics 2020Quote: ... MCF7 cells were ordered from ATCC and cultured in EMEM containing 0.01 mg/ml of human insulin (Millipore-Sigma 91077C). MCF10A cells were ordered from ATCC and cultured in DMEM/F12 containing 5% horse serum (Thermo Fisher 16050130) ...
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bioRxiv - Genomics 2020Quote: Twenty-four well plates were coated with: human or rat tail collagen type I (88 ng per well; Sigma-Aldrich), human collagen type III (88 ng per well ...
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ArchR: An integrative and scalable software package for single-cell chromatin accessibility analysisbioRxiv - Genomics 2020Quote: ... MCF7 cells were ordered from ATCC and cultured in EMEM containing 0.01 mg/ml of human insulin (Millipore-Sigma 91077C). MCF10A cells were ordered from ATCC and cultured in DMEM/F12 containing 5% horse serum (Thermo Fisher 16050130) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were plated on either tissue culture plastic (TCP) or hydrogel substrates coated with 200µg/ml human plasma fibronectin (Corning, NY, USA and Millipore, Germany) at a density of 5,000 to 15,000 cells/cm2.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was harvested for cytokine detection using MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay (Millipore) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: The DNA construct coding for the motor domain of human kinesin-5 (Eg5; residues 1–368) was cloned into a modified pET16b (Novagen) vector for bacterial expression to achieve an N-terminally 6x His tag and TEV cleavage sequence ...
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bioRxiv - Biochemistry 2020Quote: ... An identical sample set was prepared at 5µM in a solution containing 35mg/mL recombinant human albumin (#A9731, Sigma-Aldrich) and incubated at 37°C for either 24hr or 48hr with gentle rotation ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA encoding MHC heavy chain (HLA-C*08:02 and H-2 Kb) and light chain (human β2M and mouse β2M) was synthesized (Idobio) and cloned into pET-22(b) vector (Novagen). The vectors were transformed into the E ...
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bioRxiv - Cancer Biology 2021Quote: ... Chip channels were then coated with a mixture of ECM in Dulbecco’s phosphate-buffered saline (DPBS): 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30μg/mL fibronectin (Gibco ...