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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were reduced using 1 mM DTT (Sigma) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-Protein A antibodies (Sigma, P3775, 1:5000). IRDye secondary antibodies (800CW and 680RD ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Biophysics 2020Quote: ... Fluorescence labeling was done by mixing WT BIR1 with ATTO488-M (Sigma) and TCEP at pH 6.5 ...
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bioRxiv - Bioengineering 2021Quote: ... and phytic acid (50 wt% in water) were purchased from Sigma-Aldrich. Harris haematoxylin and eosin were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... then fixed in 0.2% glutaraldehyde (50 wt. % in water, Sigma-Aldrich, 340855) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... on 2 µg/cm2 poly-l-lysine mol wt 70,000-150,000 (Sigma) coated plastic and used below passage 4 ...
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bioRxiv - Microbiology 2022Quote: ... Stationary phase bacteria were opsonized with 8% (wt/vol) mouse serum (Sigma) for 20 min and added to the bone marrow derived macrophages at a multiplicity of infection (MOI ...
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bioRxiv - Developmental Biology 2019Quote: The WT and Etv5 KO mESCs were fixed with 4% paraformaldehyde (Sigma) at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... 25 wt% N,N,N’,N’-tetrakis (2-hydroxypropyl) ethylenediamine (Sigma, 122262) and 15 wt% Triton X-100 (AppliChem ...
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bioRxiv - Neuroscience 2021Quote: ... 25 wt% N,N,N′,N′-tetrakis (2-hydroxypropyl) ethylenediamine (Sigma-Aldrich) and 15 wt% Triton X-100 (Nacalai Tesque) ...
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bioRxiv - Microbiology 2022Quote: ... The resuspension was treated with 0.3 % (wt/v) sodium deoxylate (Sigma Aldrich) and 0.6 v/v Nonidet P-40 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: An agarose solution 0.35%(wt/vol) of ultra-low melt agarose (Sigma) in dilution buffer (30 mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... 4 wt% RGD-modified MeHA was crosslinked with dithiothreitol (DTT, Sigma Aldrich) at pH 9 ...
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bioRxiv - Bioengineering 2023Quote: ... a solution of 6.4 wt% gelatin type A (300 bloom, Sigma-Aldrich), 0.5 wt% Pluronic F-127 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... containing 2.5% wt/vol 1,4-diazabicyclo [2.2.2] octane (DABCO, D27802, Sigma-Aldrich). Samples for 3D-structured illumination microscopy (3D-SIM ...
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bioRxiv - Biophysics 2024Quote: ... and 3% (wt/vol) hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Sigma, 410896) in ethanol ...
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bioRxiv - Cancer Biology 2019Quote: ... CUBIC reagent 1 was prepared as a mixture of 25 wt% N,N,N,N’-tetrakis (2-hydroxypropyl) ethylenediamine (Sigma-Aldrich, 122262), 25 wt% urea (Carl Roth ...
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Empowering Engineered Muscle Function by Extending Connexin 43 Duration with Reduced Graphene OxidesbioRxiv - Bioengineering 2021Quote: ... The coating solution was prepared by adding 1 mg of fluorescein sodium salt powder in 10mL of 0.5 wt % polyvinylpyrrolidone (PVP, Sigma-Aldrich, Mw = 360,000)/ethanol solution ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast cells were grown in rich medium (YPD; 1% yeast extract [Difco, 10215203], 2% peptone [Difco, 211705], and 2% glucose [wt/vol]) (Sigma-Aldrich, G7021) on an orbital shaker (200 rpm ...
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bioRxiv - Immunology 2023Quote: ... coli and purified to >95% purity following our established protocols38 and are used throughout except WT HEL from Sigma (1 μg/ml) is used in S2F ...
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bioRxiv - Biochemistry 2022Quote: ... were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt, Aldrich) or negatively charged 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS, Sigma-Aldrich, Inc) containing N,N’-methylenebisacrylamide (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... All steps from organoid retrieval through fixation were conducted with tubes and pipette tips pre-coated in 1% (wt/vol) PBS–BSA (Sigma Aldrich; A9647), to prevent organoid loss ...
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bioRxiv - Molecular Biology 2020Quote: The purified LdCsm complex (100 μl) was first mixed with l00 μl Trizol agent (Sigma), and then 200 μl chloroform:isoamylalcohol (24:1 ...
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bioRxiv - Developmental Biology 2020Quote: ... 15 mM EDTA and 10% glycerol) supplied with 8 mM vanadyl ribonucleoside complex (Sigma-Aldrich), 50 U/ml SUPERase-In (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... The ABC Peroxidase complex was visualized using 3,3’-Diaminobenzidine tetrahydrochloride hydrate (DAB, Sigma Aldrich, D5637) for 5 minutes at RT ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
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bioRxiv - Cancer Biology 2020Quote: ... 18 μL of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane complex solution) (Sigma-Aldrich) and one drop of cross-linking modulator (2,4,6,8-Tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane ...
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bioRxiv - Biochemistry 2020Quote: ... The complex was then concentrated to 3-6 mg/ml (Amicon 100 kDa cutoff, Millipore) and loaded onto a size-exclusion column equilibrated with 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... mPSF:PAP complex was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 100 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2020Quote: Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
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bioRxiv - Molecular Biology 2023Quote: ... Fractions containing intact complex were concentrated using an Amicon 100,000 MWCO centrifugal filter unit (Millipore). The concentrated sample was applied to a Superose 6 Increase 10/300 GL (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... the SEC-purified CerS6-Nb22 complex was incubated with 120 µM fumonisin B1 (Sigma-Aldrich) for 10 minutes on ice prior to protein concentration and subsequently for an additional 2h on ice prior to grid preparation ...
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bioRxiv - Biochemistry 2024Quote: ... The purified complex was concentrated using a 10-kDa molecular mass cutoff Centricon tube (Millipore) to a final concentration of 6 mg/ml for crystallization trials ...
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bioRxiv - Immunology 2023Quote: ... MDMs were also pre-treated for 1h with a TLR3/dsRNA complex inhibitor (Merck Millipore) 5 µM final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS and 200 ug/ml Digitonin (D141-100MG ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-RBFOX1 (1:2000 for mouse and 1:1000 for human samples, Merk Millipore, MABE985), mouse anti-RBFOX2 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS ...
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bioRxiv - Immunology 2023Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.1% Triton X-100 dissolved in TBS ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35±3 days25 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were mixed with 200uL of pre-washed slurry of a 1:1 mix of EZview Red Protein G and EZview Red Protein A bead affinity gel slurry (Sigma-Aldrich) and rotated at 4C for 1hr ...
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bioRxiv - Cell Biology 2023Quote: ... protein inhibitor cocktail (1:100) and Benzonase nuclease (Novagen, 70746, 1:100) in PBS ...
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bioRxiv - Immunology 2020Quote: ... Peptides were separated from the HLA molecule remnants by ultrafiltration employing 3 kDa and 10 kDa Amicon filter units (Merck Millipore) for HLA-I and HLA-II ...
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bioRxiv - Immunology 2020Quote: Cell lines (HEK293T, HEK293, 3C11, 143B, MDCK-SIAT1, MDCK-PR8) were maintained in DMEM (Sigma Aldrich) supplemented with 10% FCS (Sigma Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells overexpressing Flag-AurkB was treated with 10 µM of trichostatin A (TSA) (Sigma-Aldrich) or DMSO for 12 hours ...
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bioRxiv - Bioengineering 2021Quote: ... for HEK293-T and SW480 cells were coated with fibronectin bovine plasma (F1141-2MG; Sigma Aldrich) before seeding cells onto the 8-well plates ...
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bioRxiv - Genomics 2021Quote: ... HEK293 and NSC were transfected with STARR-seq plasmid containing enhancer regions using polyethylenimine (PEI, Sigma) or Lipofectamine™ Stem Transfection Reagent (Thermo Scientific ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were seeded in 8-well Nunc Lab-Tek II chamber slides (0,7 cm2, Sigma) at a density of 70.000 cells/cm2 ...