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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Synchronized feeding third instar female larvae were sorted and placed in a hole created at the centre of an agar plate (3.3% wt/vol) filled with yeast paste containing 4% (w/v) FD&C Blue dye # 1 (Sigma Aldrich). The larvae were allowed to feed in the dyed-yeast paste for 2 hours ...
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bioRxiv - Microbiology 2019Quote: ... the biofilm-covered slides were gently rinsed in 1% (wt/vol) phosphate-buffered saline solution (Sigma-Aldrich, St. Louis, MO) and placed in Petri dishes (28) ...
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bioRxiv - Bioengineering 2021Quote: ... at −20°C for 6 minutes followed by a 1-hour incubation in blocking buffer containing 10 wt% BSA (Sigma) in PBS as previously described7 ...
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bioRxiv - Biochemistry 2021Quote: ... ACER3-wt or ACER3-E33G expression was induced in FreeStyle 293-F(TetR) cells with 1 µg/mL doxycycline (Sigma). Induced cells were counted and resuspended at 0.5×106 cells/mL in medium containing vehicle (0.0125% DMSO ...
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bioRxiv - Cell Biology 2020Quote: Total protein from confluent passage 1 Sirt5−/− and WT chondrocytes cultured in 6 cm dishes were collected in RIPA buffer containing 20 mM nicotinamide (Sigma) and protease inhibitors cocktail (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... For in vitro deletion of floxed alleles of Rc3h1fl/fl;Rc3h2fl/fl;CD4Cre-ERT2;rtTA3 (but with no effect on the Cre-deficient WT control Rc3h1fl/fl;Rc3h2fl/fl;rtTA3) CD4+ T cells were treated with 1 μM 4′OH-tamoxifen (Sigma) for 24h prior to activation at a cell density of 0,5-1×106 cells/mL ...
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bioRxiv - Genomics 2021Quote: ... Three mL of samples were first denatured with SDS to a final concentration of 1% (wt/vol) and 2.7 mL of preheated acid phenol (65°C) (Sigma-Aldrich). Mixtures were vortexed for 5 min at 65°C ...
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bioRxiv - Neuroscience 2023Quote: Refractive index matching was achieved by incubating the cleared brains in CUBIC-R+(M) (45 wt% 2,3-dimethyl-1-phenyl-5-pyrazole [Antipyrine; #10784, Sigma-Aldrich, Germany] ...
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bioRxiv - Developmental Biology 2021Quote: ... were soaked overnight with human SDF-1 3.3 μg ml−1 (Sigma-Aldrich, S1577) at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
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bioRxiv - Immunology 2021Quote: ... The detached peptides were separated from HLA molecules using 3 kDa cut-off centrifugal ultrafilters (Millipore, MO, USA), and then concentrated using vacuum centrifugation.
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bioRxiv - Neuroscience 2020Quote: ... and 1% protein phosphatase inhibitor cocktail (Sigma). Lysed tissues were incubated at 4°C for 20 min and centrifuged at 15,000 rpm for 15 min ...
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bioRxiv - Genetics 2021Quote: ... PolyUbiquitinated proteins (1/1000, Sigma 04-262), p62 (1/1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection of proteins was performed by using primary rabbit anti-human hepsin antibody (Sigma Aldrich, Steinheim, Germany), pAKT (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Biochemistry 2023Quote: The in vitro phosphorylation is conducted using the human Src-GST protein (Sigma-Aldrich, Catalogue number: S1076) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with Protein A Agarose/Salmon Sperm DNA and 5 μg human IgG (Sigma, Cat# I4506) or PPAR-γ Antibody (E-8 ...
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bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Biophysics 2021Quote: ... The his-tag affinity purified stator was concentrated to 1 ml using a 100 K MWCO Amicon device (Millipore). The samples were subjected to size exclusion chromatography using Enrich SEC650 column (Bio rad ...
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... The 8 ml supernatant was applied to 1 ml packed volume of Ni-NTA His-Bind Resin (Millipore Sigma). Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad) ...
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bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
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bioRxiv - Cell Biology 2020Quote: ... FA solution (12 μL, 37 wt. % in water, Sigma-Aldrich, #F8775) and HCl (0.5 ml ...
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bioRxiv - Biophysics 2020Quote: ... A small amount of depletant (0.3 wt%, 15 centipoise methylcellulose, Sigma) crowds the F-actin to a thin layer near the surface ...
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bioRxiv - Developmental Biology 2019Quote: WT and TH- adult zebrafish were fixed in 4% paraformaldehyde (Sigma) overnight at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... coated with poly-L-lysine (mol. wt > 350 kDa, Sigma-Aldrich) and cultured for up to 10 days in Nbactive4 (BrainBits® ...
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bioRxiv - Bioengineering 2019Quote: ... The aqueous solution consisted of 0.15% wt rhodamine B (Sigma-Aldrich) and 0.25% wt direct red 81 (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... membranes with cells were fixed with 2.5 wt% glutaraldehyde (Sigma Aldrich) at 4°C overnight ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... and titration of hydrogen peroxide (50 wt. % in H2O, Sigma 516813) until desired oxygen concentration was reached ...
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bioRxiv - Bioengineering 2022Quote: ... in a 0.9 % wt/vol solution of NaCl (Sigma-Aldrich, #S5886) and then filtered through a 0.22 µm syringe filter (Sarstedt ...
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bioRxiv - Developmental Biology 2019Quote: WT and Etv5 KO mESCs were fixed with 4% paraformaldehyde (Sigma) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 2% (wt/vol) sucrose (Sigma-Aldrich, St. Louis, MO) and 1% (wt/vol ...
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bioRxiv - Bioengineering 2021Quote: HiPCO SWCNTs (NanoIntegris) were suspended in 2 wt% SC (Sigma-Aldrich), using bath sonication (80 Hz for 10 minutes ...
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bioRxiv - Pathology 2021Quote: β-aminopropionitrile (0.5% wt/vol, Cat# A3134, Millipore-Sigma, St. Louis, MO) was administered through drinking water at 4 weeks of age.3 BAPN drinking water was replaced twice a week.
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bioRxiv - Pathology 2021Quote: β-aminopropionitrile (0.5% wt/vol, Cat# A3134, Millipore-Sigma, St. Louis, MO) was administered through drinking water at 4 weeks of age.3 BAPN drinking water was replaced twice a week.
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bioRxiv - Microbiology 2020Quote: ... The coding sequence for WT VxrB was cloned into pET15b (Novagen) into the NdeI and BamHI restriction sites ...
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bioRxiv - Cell Biology 2020Quote: ... with 0.4%(wt/vol) collagenase D (Sigma, cat. no. C0130-1G), 50 U/mL DNAse I (Sigma ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... WT and S265D mutant TIPRL1 were subcloned into pET15b (Sigma, #69661) for recombinant protein production and metal affinity purification as described (Sents et al ...
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bioRxiv - Bioengineering 2023Quote: ... cells were permeabilized using 0.3 wt% triton X-100 (Sigma-Aldrich) in PBS for 30 min and exposed to blocking buffer (2 wt% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... Xanthan gum (x. gum) (0.05 wt%) (Sigma-Aldrich, St. Louis, MO) was dissolved in optimized co-culture media composed of FibroLife serum-free media and DermaLife Calcium-Free basal media (LL-0029)20 in a 3:1 ratio and supplemented with fibroblast and keratinocyte growth21 ...
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bioRxiv - Microbiology 2024Quote: ... stained with 2 mL 0.1% (wt/vol) crystal violet (Sigma-Aldrich) solution for 20 min at room temperature ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Microbiology 2020Quote: ... membranes were probed with 1 in 10,000 dilution of human anti-SIC followed by 1 in 50,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich). To directly compare detection of SIC fragments by purified human and rabbit anti-SIC antibodies ...
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bioRxiv - Biophysics 2021Quote: ... and the complex formation was initiated by addition of 25 mU/mL Apyrase (Sigma), 20 mg/mL Nb35 and 10 μM peptide (GenScript) ...
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bioRxiv - Plant Biology 2019Quote: ... The antibody-antigen complex was detected using HRP conjugated Immobilin Western Chemiluminescent substrate (Millipore). For JAZ3 degradation experiments in N ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2% vanadyl ribonuceloside complex] and incubated with 50 μL of Flag M2 agarose (Sigma) for 3 h at 4°C with end-over- end rotation ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with phosphatase inhibitors complex 2 and 3 (Sigma Aldrich, P5726-1ML, P6044-1ML), DTT 10 mM and protease inhibitor cocktail (Sigma-Aldrich ...