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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... only Group-3 animals were treated with 2 mg/kg Dexamethasone sodium phosphate (Sigma Aldrich) in PBS by subcutaneous injection three times ...
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bioRxiv - Physiology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay from Sigma (St. Louis, MO), and D-luciferin from Gold Biotechnology (St ...
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bioRxiv - Developmental Biology 2020Quote: ... 2-3 fillets of late foraging larvae were incubated in M3 insect medium (Sigma-Aldrich) supplemented with 2 mM of trehalose (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% 2-mercaptoethanol) containing 10 µM SB431542 (R&D) and 3 µM CHIR99021 (Sigma), and was subsequently replaced every day ...
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bioRxiv - Cell Biology 2021Quote: ... coated tissue culture dishes and routinely sub-cultured every 2–3 days using accutase (Sigma) for detachment ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in flow cytometry buffer (FB: 2% FCS, 3 nM EDTA (Sigma, E9884) in PBS) ...
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bioRxiv - Biophysics 2021Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were procured from Sigma Aldrich, St ...
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bioRxiv - Microbiology 2022Quote: ... and 2-Heptyl-3-hydroxy-4(1H)-quinolone (pqs, Sigma-Aldrich CAS# 108985-27-9) alone and in combination ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay (Sigma). The absorbance was measured using a Synergy™ 4 plate reader (Bioteck ...
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bioRxiv - Cell Biology 2022Quote: ... then immersed in 100% ethyl-3-phenylprop-2-enoate (ethyl cinnamate [ECi]) (W243000, Sigma-Aldrich) and finally kept at room temperature until subsequent imaging step.
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bioRxiv - Cell Biology 2022Quote: ... MTT reagent [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma-Aldrich, St. Louis, MO), dissolved in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... 2 M NaCl using a 3 kDa MWCO Amicon Ultra Centrifugal Filter Unit (Merck Millipore) and mixed with tailless Xl histone octamer in the same buffer at a molar ratio 2:1 APLFAD-Δ:histone octamer on ice ...
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bioRxiv - Neuroscience 2022Quote: ... and 1% each of Phosphatase Inhibitor Cocktails 2 and 3 (Millipore Sigma #P5726 and #P0044). Cells were scraped ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for 3 hours in a blocking solution containing 2% BSA fraction V (Sigma), 0.1% TritonX-100 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... was used at 1 µM and ARP 2/3 complex inhibitor - CK666 (182515; Sigma-Aldrich) was used at 100 µM concentration ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were air dried for 10 minutes and then freshly prepared 2% 3-aminopropyltriethoxysilane (Sigma) in acetone was applied for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... and 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) for 3 min (1:5000, Sigma Aldrich), and mounted in prolong gold (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... a standard 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT; Sigma-Aldrich) was employed.
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bioRxiv - Cell Biology 2023Quote: ... plates were treated with 3-amino-propyltrimethoxysilane (diluted 1:2 with PBS; Sigma-Aldrich/Merck) for 15min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% each of phosphatase inhibitor cocktails 2 and 3 (Sigma, Saint Louis, MO, USA). Particulate was removed by centrifugation of lysates at 13,000 rpm for 10 minutes at 4°C and filtration through 0.45 µm syringe filters and lysates were stored at -80°C until preparation for MIB-MS ...
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bioRxiv - Biophysics 2023Quote: Thoraces from 2–3-day-old adult Drosophila were dissected in 4% paraformaldehyde (PF, Sigma))) ...
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bioRxiv - Neuroscience 2024Quote: ... 2’-O-Dibutyryladenosine 3’,5’-cyclic monophosphate sodium salt (cAMP; 50 μM, Millipore Sigma, D0627), and cis-4 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... ground and homogenized western corn rootworm larval tissue (3-7 larvae per biological replicate, n=8-11) using the GenElute Universal Total RNA Purification Kit (Sigma-Aldrich, St. Louis, MO, USA). A NanoDrop spectrophotometer (ND-1000 ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7) gradient (20%, 40%, 60%, 80%, 100%), incubated 3 times (1h, o/n, 2h) in 100% dichloromethane (DCM) (Sigma-Aldrich, St. Louis, MO, USA) to remove hydrophobic lipids ...
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bioRxiv - Physiology 2020Quote: ... at 4°C for 60 min using an Amicon Ultra-4 3 kDa centrifugal filter device (Merck Millipore). The 50 μL retentate was the final volume of concentrated EVs ...
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bioRxiv - Neuroscience 2020Quote: ... tissues were washed 3 times in PBS and stored at 4°C in PBS containing 0.05% NaN3 (Sigma) until use ...
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bioRxiv - Microbiology 2021Quote: ... blocked for 20 min at 37 °C in PBS−/− with 3% w/v bovine serum albumin (BSA) (Sigma), incubated at 37 °C for 1 h with a 1:333 dilution of 1 mg/ml anti-VE-cadherin polyclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2019Quote: Gaboxadol (4,5,6,7-retrahydroisoxazolo[5,4-c]pyridin-3-ol hydrochloride, THIP hydrochloride; Sigma Aldrich, St. Louis, MO, USA, # T101) was dissolved in dH2O at 1 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampi were first lysed in 20 volumes of homogenizing buffer (pH 7.3 solution of 0.32 M sucrose and 0.005 M Na3PO4) at 4°C supplemented with protease inhibitors (Sigma), 50 mM sodium fluoride ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were enzymatically dissociated for 20 min at 37°C with papain (3 U/mL, Sigma-Aldrich), diluted in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2022Quote: ... female 3–4-week-old BALB/c or C57BL/6 mice were injected intraperitoneally with 10mg azoxymethane (Sigma) per kg mouse weight ...
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bioRxiv - Plant Biology 2022Quote: ... the same total protein extracts were incubated with Endoglycosidase H at 37 °C for 3 h (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated for 3 h at 22°C in Anti-mono-ADP-Ribose Binding Reagent (Sigma, MABE1076 ...
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bioRxiv - Molecular Biology 2019Quote: ... and immunoprecipitated overnight at 4 °C with 2 µg affinity-purified rabbit anti-PKM2 antibody raised against a C-terminal peptide (Sigma cat. # SAB4200105, Lot #030M4874) pre-coupled to Dynabeads sheep M-280 anti-rabbit IgG beads (LifeTech) ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant became the soluble fraction and the pellet was solubilized in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Millipore Sigma 11873580001), 0.5 mM PMSF ...
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bioRxiv - Neuroscience 2021Quote: ... during 7 days, in a medium composed of equal volumes of modified C2C12 differentiation medium (DMEM, 2% horse serum (Sigma-Aldrich, #H1270),1% antibiotic-antimycotic (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Oral microgavage of 6 dpf larval zebrafish was performed using pH indicators m-cresol purple (0.2%; Thermo-Fisher, 2303-01-7) or phenol red (2%; Sigma, 143-74-8). The fish were then imaged on a Leica MZ10F stereoscope using a Leica MC190 HD color camera ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral supernatants were generated by transfecting the plasmids into 293T cells and used to infect 8988T cells that were then selected for at least 7 days with 2 μg ml−1 puromycin (Sigma-Aldrich, P8833). The RNAi consortium clone IDs for the hairpins used were ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
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bioRxiv - Molecular Biology 2022Quote: ... Stimulation of Jurkat cells for 2-7 hour experiments was achieved with 50ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Alrich, P8139) and 500ng/ml ionomycin calcium salt from Streptomyces conglobatus (ionomycin ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 1 ml of RPMI + 2% Bovine Serum Albumin (BSA, heat shock fraction, protease free, essentially globulin free, pH 7, Sigma, Catalog #A3059) to a concentration of ∼2 x 108 CFU/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were fixed for 7 minutes in 2% formaldehyde/0.2% glutaraldehyde solution at room temperature and further incubated with X-Gal (Sigma, Cat. no. B4252) solution for one hour at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... incubated with anti-Flag M2 Magnetic beads (4 °C for 2 h; Sigma, Cat# M8823), washed 5 times and then eluted with 3xFLAG peptide ([0.1 mg/mL] ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were treated with 2 μM cytosine β-D-arabinofuranoside hydrochloride (Ara-C; Sigma Aldrich) on day 4 to reduce the proliferation of non-neuronal cells ...
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bioRxiv - Physiology 2019Quote: ... at 27°C in 2 mL BSA-free ECS with 1 μL 20% Pluronic (Sigma), followed by 20 min in ECS with 0.1% BSA to de-esterify the dyes ...
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bioRxiv - Bioengineering 2022Quote: ... at 4°C for 2 h and blocked with 1% bovine serum albumin (Sigma-Aldrich), 3% donkey serum (Millipore) ...
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bioRxiv - Genomics 2019Quote: ... to which 2 ml of pre-chilled Buffer C (Nuclei PURE Lysis Buffer (Sigma-Aldrich), 25 μM α-amanitin ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated 30 min at 37°C with Dihydrorhodamine (DHR) 123 (2 µM, Sigma) in complete medium ...
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bioRxiv - Cell Biology 2022Quote: ... for 30 min at 37°C and blocked with 2% Bovine Serum Albumin (Sigma-Aldrich) in PBS for 1hr at 37°C or at 4°C overnight ...
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bioRxiv - Genetics 2023Quote: ... 1 µg ml–1 natural mouse laminin and 2 µM arabinosylcytosine (Ara-C, Sigma-Aldrich). After 48 h ...