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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for 3 3 17 17 Bis ethylenedioxy 19 hydroxyandrost 5 ene 19 d2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... At DIV2-3 the medium was supplemented with 5 µM AraC (Sigma-Aldrich, C1768) to prevent glial overgrowth.
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bioRxiv - Cell Biology 2023Quote: SKOV-3 cells were treated with indicated concentrations of 5-Aza-CdR (Sigma, UK) and SAHA (LC laboratories ...
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bioRxiv - Bioengineering 2022Quote: ... + 0.5 mM 8-bromodenosine 3’,5’-cyclic monophosphate (8-Br-cAMP; Sigma-Aldrich B5386) or (ii ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP, 11383221001, Merck-SIGMA). Sections were mounted using DPX (6522 ...
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bioRxiv - Microbiology 2022Quote: ... 0.1mM 8- Bromoadenosine 3′,5′-cyclic monophosphate (8-Br-cAMP) (Sigma-Aldrich, Cat. B5386), 0.1mM 3-Isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Plant Biology 2022Quote: ... 5-bromo-4-chloro-3’-indolyphosphate p-toluidine (BCIP) (Sigma-Aldrich catalog number B6149) staining was performed ...
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bioRxiv - Bioengineering 2023Quote: The mice were anaesthetized with isoflurane (induction 5%, maintenance 2−3%; Sigma-Aldrich, USA) and secured in a stereotaxic instrument (RWD ...
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bioRxiv - Bioengineering 2023Quote: ... 1μM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (cAMP; Sigma-Aldrich, D0260), 100ng/ml biotin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... before 3 × 10 min washes with 5% milk 0.05% Tween-20 (Sigma, P1379-250ML). Secondary antibody incubations were carried out for 1 h at room temperature before 3 x washes with PBS containing 0.05% Tween-20 ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... and substrates 5-bromo-4-chloro-3’-indolyphosphate/nitro-blue tetrazolium (BCIP/NBT, Sigma). We used CTL-ImmunoSpot S6 Micro Analyzer to visualize and quantify IFNγ+ MAIT cell spots ...
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bioRxiv - Biochemistry 2023Quote: ... and adenosine 5’-diphosphate sodium salt (ADP) (3 μM) (20398-34-9, Sigma Aldrich) were dissolved in phosphate buffered saline (PBS ...
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bioRxiv - Genetics 2023Quote: ... beads were rewashed 3 times 5 minutes with 1× wash buffer (Sigma-Aldrich, W0390) on a rotator and with protease and phosphatase inhibitors ...
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bioRxiv - Developmental Biology 2023Quote: ... and 100 nM 8- Bromoadensoine 3’,5’-cyclic monophosphate sodium salt (Sigma, Cat#B7880) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... from QIAGEN or costume siRNA against MdmX (siMdmX#1, sequence: 5’-AGAUUCAGCUGGUUAUUAA-3’) from Sigma-Aldrich. For Spry4 knockdown cells were transfected with a pool of 3 siRNAs against Spry4 (s37824 ...
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bioRxiv - Microbiology 2024Quote: ... 50 µg/ml of 3-5 kDa Fluorescein isothiocyanate-dextran (FITC-dextran, Sigma-Aldrich) was added to the apical channel media on the day before Salmonella Typhimurium infection ...
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bioRxiv - Genomics 2024Quote: ... blocked for 3 hours at 38°C in 5% BSA (Sigma #A6947 (LOT #SLBZ6761)) in 0.5% TBS-Triton X100 (Sigma-Aldrich #X100 ...
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bioRxiv - Neuroscience 2024Quote: ... 2’-ODibutyryladenosine 3’,5’-cyclic monophosphate sodium salt (cAMP; 50 μM, Millipore Sigma, D0627), and cis-4 ...
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bioRxiv - Molecular Biology 2020Quote: ChIP assay was used to detect the enrichment of HDAC3 in the promoter region of the miR-17-92 cluster and that of p65 in the Pgf promoter region using the EZ-Magna ChIP TMA kit (Millipore). The lung fibroblasts of each group in logarithmic growth phase were cross-linked and cultured with 1% formaldehyde for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were overexpressed in E.coli BL21 (DE3) at 17 °C overnight and then purified by Glutathione-agarose (Sigma #G4510). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... The water was then removed and replaced with 100 μL of eliciting solution containing 17 mg/mL luminol (Sigma Aldrich), 200 μg/mL horseradish peroxidase (Sigma Aldrich) ...
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bioRxiv - Systems Biology 2020Quote: ... were used for binding assays with a constant amount of fluorescein isothiocyanate (FITC)-conjugated peptides (resuspended in 17 mM Tris-HCl pH 8, 100 mM NaCl and 0.5% Brij L23 (Millipore Sigma), peptides final concentration of 10 nM) ...
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bioRxiv - Molecular Biology 2021Quote: D7+17 organoids were exposed to 10% (v/v) plasmapheresis plasma supplemented with anticoagulants heparin (100 μg/ml, Sigma Aldrich) and PPACK (10 μM ...
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bioRxiv - Genomics 2020Quote: CTCF Chromatin immunoprecipitation (ChIP) was performed on 1 × 107 Ter119+ erythroid cells using a ChIP Assay Kit (Millipore: 17-295) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: The assay was performed using the In Vitro Tubulin Polymerization Assay Kit (≥99% Pure Bovine Tubulin; 17-10194 Sigma-Aldrich), according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2020Quote: Polysaccharides were resuspended at a concentration of 5 mg/mL in 50 mM HEPES (pH 7.8) using heat and sonication with the exception of maltodextrin (DE 13-17; Sigma Aldrich) which was resuspended at 50 mg/mL ...
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bioRxiv - Microbiology 2020Quote: ... Benztropine mesylate (CAS No. 132-17-2) and Bafilomycin A1 (CAS No. 88899-55-2) were purchased from Sigma-Aldrich. Active pharmaceutical ingredients for an additional 59 drugs ...
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bioRxiv - Neuroscience 2022Quote: ... additionally filtered with a 735 nm LP filter eliciting an isomerization rate of ~17 R*/s) in oxygenated Ames’ medium (Sigma) with constant bubbling (95% O2 ...
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bioRxiv - Developmental Biology 2022Quote: Wildtype and Nek2 knockout cells were treated with 0.5 μM RA as described [37] for 17 days on poly-L-lysine hydrobromide (Sigma, P5899) coated coverslips ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The MeRIP assay was carried out using the RNA-Binding Protein Immunoprecipitation Kit (Cat# 17-701, EZ-Magna, Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were immunoprecipitated with 2 μg of IgG (negative control) or Smad3 antibody in coimmunoprecipitation reagents (17-195, Millipore). Semi-quantitative and quantitative PCR were performed to amplify α-SMA or Col1A promoter regions containing SBE.
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bioRxiv - Developmental Biology 2020Quote: HLECs were treated with chemicals as described above and their intracellular permeability was analyzed using In Vitro Vascular Permeability Imaging Assay (Cat# 17-10398, Sigma) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... and then purified by mixing 17 mL PBS brain lysate with 4,5 mL Percoll Plus (Sigma, Saint Louis, Missouri, USA). After 15 min 500g centrifugation in +4 □C ...
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bioRxiv - Neuroscience 2021Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore #17-700) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Citrulline-containing proteins were modified on the membrane and detected using the anti-modified citrulline detection kit (Millipore, 17-347) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: RIP assay was performed following the manufacturer’s instructions (EZ-Magna RIP RNA-binding Protein Immunoprecipitation Kit, Sigma-Aldrich, #17-701). Antibody against eIF4G was used for the part of immunoprecipitation ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in this study: monoclonal mouse antibody to BIN1 (clone C99D against exon 17) from Millipore, polyclonal rabbit antibody to IRSp53 (BAIAP2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-PP1α antibody (2 μg) was added to the supplied spin column (Catch and Release Immunoprecipitation Kit; cat no. 17-500; Millipore) along with the cell lysates (500 mg ...
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bioRxiv - Microbiology 2024Quote: ... The pesticides were: 3,6-dichloropyridine-2-carboxylic acid (i e. clopyralid, pesticide analytical standard, CAS 1702-17-6, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Evolutionary Biology 2024Quote: Mouse and macaque endometrial epithelial organoids were treated for two consecutive days with 17-ß estradiol (E2) (Sigma Aldrich, E8875) at 10 nM or for two days with 17-ß estradiol (E2 ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 and 7 post IL-12mRNA/scRNA injection (day 11, 13 and 17 post inoculation) and digested in 30% collagenase (Sigma) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, #17-700) following the manufacturer’s instructions ...
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SPNS1 is required for the transport of lysosphingolipids and lysoglycerophospholipids from lysosomesbioRxiv - Biochemistry 2022Quote: ... control and gSpns1-cKO mice were injected with 4 μl/g body weight of a 17% (w/v in 0.9% NaCl) Triton WR1339 solution (Sigma Aldrich) for three days prior to fractionation of lysosomes ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were incubated with F12K and DMEM media supplemented with 17 mM [U-13C6]glucose (Cambridge Isotopes) or unlabeled naturally occurring glucose (Sigma) (standard concentration in F12K/DMEM media ...
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bioRxiv - Immunology 2023Quote: ROS staining was performed by incubating cells with dihydroergotamine 123 (17 mg/ml) diluted in Hank’s balanced salt solution with 10mMHEPES (all from Sigma Aldrich) for 15 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Media was replaced with fresh MEM without phenol-red (Corning, 17-305-CV; supplemented with 1% sodium pyruvate (Sigma, S8636), 1% HEPES ...
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bioRxiv - Neuroscience 2024Quote: ... Primers were designed to target Nr3c1 exon 17 using PyroMark Assay Design software and synthesised by Sigma Aldrich (HPLC purified). The reverse primer was 5’ end biotin labelled ...
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bioRxiv - Genomics 2024Quote: ... slides were then washed four times in milli-Q water before being stained with Mayer’s Hematoxylin for 17 minutes (Sodium hydrosulfite, Cat No. 157953-5G, Sigma Aldrich; Hematoxylin Solution ...
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bioRxiv - Cell Biology 2024Quote: Glass coverslips were glued to the bottom of a petri dish with a 17 mm diameter hole where a custom-made plastic ring was attached using paraffin (Paraplast X-tra; Sigma). Agarose solution was prepared by mixing one part of 2x Hank’s buffered salt solution (HBSS ...
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bioRxiv - Microbiology 2024Quote: ... 5’-dithio-bis-(2-nitrobenzoic acid) (DTNB) (Sigma Aldrich) 100 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Then the supernatant was separated by size to a large (>3 kDa) and small (<3 kDa) fraction using Amicon ultrafiltration system with 3 kDa filter (Millipore, Ireland). The fraction then filtered again through a 0.22 µm (Millipore).