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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for 3 3 17 17 Bis ethylenedioxy 19 hydroxyandrost 5 ene 19 d2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... and crosslinked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma). Biotin-labelled probes were hybridized with sRNAs on the nylon membrane and stabilized streptavidin-horseradish peroxidase was used to detect the biotin signal.
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bioRxiv - Synthetic Biology 2023Quote: ... activated with 250mM 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma)/ N-Hydroxysuccinimide (NHS ...
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bioRxiv - Systems Biology 2024Quote: ... 5µM 6-bromoindirubicin-3-oxime / GSK-3 inhibitor (Sigma, 361550-1MG) and 100 µg/ml Primocin (InVivogen ...
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bioRxiv - Systems Biology 2024Quote: ... or 3-bromopyruvate (3-BP; Sigma-Aldrich, St. Louis, MO; 16490) were added to their respective wells ...
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bioRxiv - Biophysics 2024Quote: ... 15 mM 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide (Millipore Sigma E6383), 5 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Microbiology 2020Quote: 106 ARPE-19 cells were centrifuged at 2,000g for 10 min at 4°C and resuspended in PBS (Sigma-Aldrich, St Louis, MO). Cells were pelleted ...
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bioRxiv - Biochemistry 2021Quote: ... For the time course shown in Fig 2B 19 μl of this protein were combined with 1 μl of trypsin (Sigma; 1 mg/ml) and the mixture was incubated at RT ...
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bioRxiv - Cell Biology 2021Quote: ... 2.7 mM KCl [Sigma-Aldrich P9541], 19 mM Tris-HCl pH 7.5, and 0.1% v/v Tween 20 [Sigma-Aldrich P7949] in water) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... recordings were performed with borosilicate glass pipettes (7-15 MΩ) and filled with normal rat Ringer’s solution with 2% biocytin (Sigma, cat # 576-19-2). The pipette was positioned with a micromanipulator (Luigs & Neumann ...
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bioRxiv - Molecular Biology 2024Quote: ... for MCF-7/MDA-MB-231/ARPE-19/HEPG2/MCF10A cells and 10% (v/v) bovine calf serum (Sigma-Aldrich, Cat. No. 12133C) for NIH3T3 cells ...
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bioRxiv - Physiology 2024Quote: ... 10-week-old male WT and Lgr4cko mice were injected with vehicle (sodium citrate, pH 4-4.5) or low-dose (40mg/kg body weight) (19, 57, 58) STZ (Sigma Aldrich, St Louis, MO) for five consecutive days ...
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bioRxiv - Plant Biology 2020Quote: ... water was removed and replaced by 100 µl of the assay solution: 17 µg/mL Luminal (Sigma-A8511, USA), 10 µg/ml Horseradish peroxidase (HRP ...
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bioRxiv - Developmental Biology 2021Quote: ... This solution was overlaid on top of a gradient (17%, 25%, 40%, 60%) of Iodixanol (Millipore Sigma, cat. #D1556) and ultracentrifuged at 46,500 rpm (Beckman Coulter VTi50 ...
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bioRxiv - Neuroscience 2020Quote: ... Vaginal plugs were assessed and pregnant mice with gestation day (GD) 17 were injected intravenously (i.v.) with 5mg/kg PolyI:C (Polyinosinic-polycytidylic acid; P9582, Sigma, USA). Aliquots of 5mg/ml were prepared by resuspending in the sterile 0.9% saline and were stored at −20°C until further use ...
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bioRxiv - Neuroscience 2020Quote: PP2A activity was measured by using immunoprecipitation phosphatase assay kit according to the manufacturer’s instructions (Catalog # 17-313, Millipore). Statistical differences were analyzed using post hoc test with Bonferroni’s correction following one-way ANOVA ...
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bioRxiv - Plant Biology 2021Quote: ... The following day the water was replaced with a solution containing 17 μg mL−1 (w/v) luminol (Sigma), 10 μg mL−1 horseradish peroxidase (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... luciferase activity was determined after addition of luciferin/lysis buffer (10 mM MgCl2, 0.3 mM ATP, 0.5 mM Coenzyme A, 17 mM IGEPAL (all Sigma-Aldrich), and 1 mM D-Luciferin (GoldBio ...
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bioRxiv - Microbiology 2022Quote: RNA immunoprecipitation (RIP) assays were performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (EMD Millipore 17-700). HEK293T cells were plated on 15-cm dishes coated with poly-L-ornithine at a density of 4E4 cells/cm2 ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were titrated by infection of 293T-ACE2 cells and luciferase activity was determined after a 48-hour incubation at 37°C and 5% CO2 by addition of luciferin/lysis buffer (10 mM MgCl2, 0.3 mM ATP, 0.5 mM coenzyme A, 17 mM IGEPAL CA-630 (all Sigma-Aldrich), and 1 mM D-Luciferin (GoldBio ...
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bioRxiv - Molecular Biology 2021Quote: RNA immunoprecipitation (RIP) experiments were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700, Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... luciferase activity was determined after addition of luciferin/lysis buffer (10 mM MgCl2, 0.3 mM ATP, 0.5 mM Coenzyme A, 17 mM IGEPAL (all Sigma-Aldrich), and 1 mM D-Luciferin (GoldBio ...
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bioRxiv - Immunology 2021Quote: ... and pSARS-CoV-2 expressing SARS-CoV-2 S protein (0.6ug) (GenBank; MN908947.3) (Brouwer et al., 2020).Transfection was performed in 293T/17 cells using genejuice (Novagen, USA) transfection kit according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... For PILO-SE induction,8,16,17 mice were injected subcutaneously (s.c.) with the muscarinic antagonist scopolamine methyl nitrate (1 mg/kg, s.c.; Sigma Aldrich) to reduce the peripheral effects of PILO ...
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bioRxiv - Cancer Biology 2024Quote: ... ND-Methyladenosine RNA immunoprecipitation (meRIP) was performed using Magna MeRIP™ m6A Kit (Cat# 17-10499, Sigma-Aldrich, UK) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Immunology 2022Quote: ... After the addition of luciferin/lysis buffer (10 mM MgCl2, 0.3 mM ATP, 0.5 mM coenzyme A, 17 mM IGEPAL CA-630 (all Sigma-Aldrich), and 1 mM D-Luciferin (GoldBio ...
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bioRxiv - Immunology 2023Quote: ... after the addition of luciferin/lysis buffer (10 mM MgCl2, 0.3 mM ATP, 0.5 mM coenzyme A, 17 mM IGEPAL CA-630 (all Sigma-Aldrich) and 1 mM D-Luciferin (GoldBio ...
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bioRxiv - Plant Biology 2024Quote: ... Antibodies were added to the beads in 50 μL ChIP dilution buffer (4 μg anti-H3K27me3, Millipore 17-622) and incubated for 1 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ChIP assays were performed using the EZ-Magna ChIP A/G Chro matin Immunoprecipitation Kit (17-10086, Merck Millipore) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... the selective D2 receptor antagonist Sulpiride (Sigma) was injected at a final estimated concentration of 400 nM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The plates were incubated for two hours at RT and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) substrate solution (Sigma-Aldrich, USA) was added for colour development ...
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bioRxiv - Microbiology 2021Quote: 5-aza-2’-deoxycytidine (5-AzadC, A3656) and epigallocatechin-3-gallate (EGCG, E4143) were purchased from Sigma Aldrich. Antibodies against CREB (sc-186) ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM DTT) and eluted with Buffer A supplemented with 5 mM DTT and 3 mM Desthiobiotin (SIGMA). Peak fractions were combined and further purified via size exclusion chromatography on a Superdex 200 (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... FW 5’ – CCA CAT GGG AGA GTC ACA T −3’ and RV 5’-ATA GCC TGG AAG CGG TCA GAT G −3’ (Sigma-Aldrich Japan, Inc., Tokyo, Japan). The PCR products (521 bp ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then dehydrated in ascending concentrations of ethanol for 5 min each (2[×[35%, 50%, 70%, 80%, 90%, 3[×[100%) and washed 3 times for 5 min with propylene oxide (Sigma-Aldrich, #cat 110205-18L-C). Next ...
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bioRxiv - Neuroscience 2024Quote: ... sections were dehydrated in increasing concentrations of ethanol for 5 min each (2 × 35%, 50%, 70%, 80%, 90%, 3 × 100%) and washed 3 x 5 min with propylene oxide (Sigma- Aldrich, #cat 110205-18L-C). Sections were next embedded overnight at RT in Durcupan resin (20g component A ...
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bioRxiv - Immunology 2020Quote: ... two STAT-3 inhibitors (STAT-3 inhibitor III (WP-1066) and STAT-3 inhibitor XIII (C-188-9) were tested (Merck, Sigma) and WP-1066 was used for infection studies at a final concentration of 12 μM ...
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bioRxiv - Plant Biology 2020Quote: ... inflorescences were soaked in 3% (w/v) 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (Sigma Chemical, St. Louis, MO, USA, E6383) with 0.05% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... 2–3 endodermal explants (2PP or 3/4PP endoderm) were combined with 2–3 mesenchymal explants on Nucleopore membrane filters (Millipore) supported by fine meshed metal grids (Goodfellows) ...
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bioRxiv - Neuroscience 2024Quote: ... antibodies were diluted in a 3% BSA in PBS and either 3% normal goat serum or 3% normal donkey serum (Millipore-Sigma) depending on the secondary antibody host species ...
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bioRxiv - Cell Biology 2020Quote: ... 14-3-3 antibody and active recombinant AMPK (α2β1γ1) were from Millipore. Precast Tris-Glycine polyacrylamide gels and 10X tris-glycine gel electrophoresis buffer were from BioRad ...
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bioRxiv - Bioengineering 2021Quote: ... 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC-HCl, Sigma Aldrich, 8510070025), chlorosulfonic acid (Sigma 571024) ...
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bioRxiv - Plant Biology 2022Quote: ... in the presence of 1 mM 3-aminotriazol (3-AT) (Sigma-Aldrich). Results were expressed in the form of a heat map for the strength of interaction according to the colony growth after five days of incubation at 30°C.
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bioRxiv - Neuroscience 2021Quote: ... and developed using 3-3’-diaminobenzidine (DAB; Sigma-Aldrich, St. Louis, MO) as the chromogen.
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bioRxiv - Microbiology 2021Quote: ... N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C12-HSL, Millipore Sigma); and a rhamnolipid mixture (RHL ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μM (2’Z,3’E)-6-Bromoindirubin-3’-oxime (Sigma, B1686), 20 μM Tideglusib (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC, 7.00 g; Sigma) (molar ratio of NHS:EDC = 1:2 ...
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bioRxiv - Immunology 2024Quote: ... HRP activity was detected with SIGMAFAST 3-3’Diaminobenzidine tablets (Sigma-Aldrich), whereas alkaline-phosphatase activity was detected using naphtol AS-MX phosphate and fast blue salt with levamisole (All from Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC-HCl; Sigma-aldrich) were added at a concentration of 0.47 and 0.95 mg mL-1 ...