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Citations for Millipore Sigma :
9251 - 9300 of 10000+ citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and extracted in 200 μl of lysis buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% n-dodecyl-D-maltoside (DDM) (Sigma), and complete protease inhibitors (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... paraformaldehyde (using a stock solution with 0.4 g paraformaldehyde dissolved in 10 ml 0.1 M sucrose with 0.6 µl 10 N sodium hydroxide [all from Sigma-Aldrich]), 0.05 M cacodylate buffer (using dimethylarsinic acid sodium salt trihydrate from Merck and HCl for pH adjustment to 6.4-7.4 ...
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bioRxiv - Microbiology 2022Quote: ... The rest of the supernatant was incubated O/N at 4°C with 8µg (per condition) of anti-NUP98 (rat, N1038 – Sigma). 62.5µl of A/G beads per condition were added and incubated for 2h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The total membranes were solubilized with a buffer containing 1×PBS (pH 7.4) and 0.5% N-lauroylsarcosine sodium (Sigma-Aldrich) for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... but without the use of iodoacetamide for cysteine alkylation due to the treatment of 50 mM N-ethylmaleimide (Sigma) during the sample immunoprecipitation ...
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bioRxiv - Neuroscience 2022Quote: ... live hippocampal cultures were incubated with an N-terminal APP antibody (mouse anti-APP 22C11, 1:500; EMD Millipore) diluted in conditioned neuronal media for 15 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 250 mM xylose or 225 mM N-acetylglucosamine) were applied to a TLC Silica gel 60 F254 (Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: ... 22 mm × 22 mm #1.5 borosilicate coverslips (DOT Scientific) were placed in a Wash-N-Dry coverslip rack (Sigma) and submerged in ∼80 mL of 1 M NaOH in a 100-mL glass beaker ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were collected and raised at either 28.5 or 24°C in N- Phenylthiourea (PTU, Sigma Aldrich, Cat# P7629)/Volvic source water (0.003% final ...
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bioRxiv - Cell Biology 2024Quote: ... and extracted in 200 μl of lysis buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% n-dodecyl-D-maltoside (DDM) (Sigma), and complete protease inhibitors (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... – N medium was prepared using 0.5x MS basal salt micronutrient solution (M0529-1L, Sigma-Aldrich, Saint Louis, MO, USA) supplemented with 1.5mM CaCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cancer Biology 2024Quote: ... Larvae were maintained in E3 containing 0.2 mM N-phenylthiourea beginning 1 day post fertilization (dpf) (PTU, Sigma Aldrich) to prevent pigment formation during imaging experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... The homogenates were then lysed on ice for 30 min in nucleus lysis buffer (HEPES 50 mM, EDTA 10 mM, N lauryl sarkosyl 0.5%, sodium butyrate 10 mM, EDTA free protease inhibitor cocktail Sigma). DNA was sheared using a Bioruptor pico from Diagenode for 10 cycles (30 sec on ...
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bioRxiv - Neuroscience 2024Quote: ... genetic A and B mixture, LGAB) and hydrochloric acid (1.0 N solution, material number: H9892, HCl) were purchased from Sigma–Aldrich (St ...
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bioRxiv - Molecular Biology 2024Quote: Synechocystis cultures (50 mL) grown under different conditions were supplemented with 10 mM N-ethyl-maleimide (NEM, Sigma-Aldrich) and further cultivated for 1 h to block free cysteine residues ...
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bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
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bioRxiv - Immunology 2024Quote: ... Ifngr1flox/flox male mice (n=4/group) were daily administrated intraperitoneally with tamoxifen (Sigma-Aldrich, St. Louis, MO, USA) solution (20 mg/mL stock solution dissolved in corn oil (Aladdin ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reconstituted visual pigments were extracted using 1% n-dodecyl b–D-maltoside (DDM) (Sigma-Aldrich, Saint Louis, MO) in 10 ml of extraction buffer (pH 6.7 ...
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bioRxiv - Biophysics 2022Quote: ... 1% DDM [n-Dodecyl-β-D- maltoside]) containing cOmplete mini protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). Lysates were mixed 1:1 with 2X LDS sample buffer containing 50 mM TCEP (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... PLL labeled with fluorescein 5-isothiocyanate (PLLFITC) and 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester (Rhod) were purchased from Sigma.
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bioRxiv - Microbiology 2023Quote: ... Pelleted cells were resuspended in 1X PBS with 100 mM N-ethylmaleimide (NEM) (Cat#128-53-0, Millipore Sigma) for 15 minutes at room temperature27 ...
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bioRxiv - Physiology 2023Quote: ... Second derivatization was with 35 µL N,O-Bis(trimethylsilyl)trifluoroacetamide (15238- 10x.1ml, Sigma-Aldrich, St. Louis MO) on thermo-shaker at 37°C for 40 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... we deployed a rapid lysis protocol using reagents from the SYBR Green Extract-N-Amp Tissue PCR Kit (Sigma) (Doan and Monuki ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 4 μl (× n samples) of monoclonal anti-Flag M2 antibodies produced in mouse (Sigma-Aldrich). The suspension was moved to 4 °C and rotated for >3 h to conjugate antibodies to magnetic beads ...
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bioRxiv - Biochemistry 2023Quote: Purified LceB was dialyzed O/N at 4 °C with the addition of 1:200 mg/mg LceB:thrombin (Sigma) to remove the N-terminal 6His-tag ...
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bioRxiv - Microbiology 2023Quote: ... 1x106 ATCC46645-H2A:mRFP and ATCC46645-H2A:mRFP Bir1OE-N conidia were inoculated in 100 mL of RPMI + 0.5 ug/mL Voriconazole (Sigma-Aldrich) and incubated at 50 RPM for 6 hrs at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: Neuroblastoma cell line SK-N-AS was maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The supernatants were then mixed with freshly prepared Griess solution consisting of 0.1% N-1-naphthylethylenediamine dihydrochloride (Sigma, #N9125) and 1% sulfanilamide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: PAW-induced outer membrane permeability was measured based on fluorescent dye N-phenyl-1-naphthylamine (NPN; Sigma-Aldrich, Australia) uptake [22] ...
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bioRxiv - Plant Biology 2023Quote: ... Spores were grown on solid 0.5× Gamborg B-5 media for 5 days and dispensed in 6-well plates containing 4 mL of liquid 0.5× Gamborg B-5 plus supplements: 0.1% N-Z amino A (Sigma #C7290) 0.03% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... and microforged glass capillaries (25 to 30µm inner diameter) filled with mineral oil (ref. n°M5904, Merck/Sigma-Alrich). 13.8nL of cell suspension from confluent T25 flasks (50×106 cells per ml approx. ...
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: Animals were randomized with the sealed envelope method to receive either rapamycin (Sigma Aldrich, 250 μg/kg, n=9) or vehicle (<5% ethanol in saline ...
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bioRxiv - Biochemistry 2023Quote: ... 0.6 % (v/v) n-dodecyl-β-D-maltoside (DDM from Inalco Pharmaceuticals) and 1.6 mg/mL iodoacetamide (Sigma-Aldrich) for 20 min at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... a solution of 50 μM αS and 1 μM N-protein in 20 mM Tris buffer (Sigma-Aldrich, UK), pH = 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... consisting of PBS supplemented with 1mM of the amino-reactive MA-NHS (Methacrylic Acid N-Hydroxysuccinimide Ester, Sigma-Aldrich). Specimens were incubated for 1-1.5 hours on gentle rocking at room temperature.
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bioRxiv - Cell Biology 2023Quote: ... The DNA corresponding to the fxn gene was amplified using KOD hot start master mix (Cat. N° 71842 Millipore) and FwDdFXNOE and RevDdFXNOE as primers (Table 1) ...
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bioRxiv - Biochemistry 2023Quote: ... the protein (with N-terminal His6 tag) was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 8.11 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA was extracted from mouse tail tip samples using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR was performed using the REDExtract-N-Amp PCR Readymix (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested and lysed in NP-40 buffer supplemented with 10 mM N-ethylmaleimide (Sigma, catalog no.: E3876). Flag-immunoprecipitations were performed as described previously ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended the cell pellet in 150 μl ice cold 650ug/mL N-Ethylmaleimide (NEM; Sigma-Aldrich, St. Louis, MO) in PBS solution ...
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bioRxiv - Molecular Biology 2023Quote: Full length Xenopus laevis Polκ with an N-terminal 6xHis-tag was amplified from pCMV-Sport.ccdb-Polκ (Williams et al., 2012) and cloned into pET28b (Novagen) using primers A and B ...
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bioRxiv - Plant Biology 2024Quote: Genomic DNA was extracted from a small piece of Arabidopsis leaf using Extract-N-Amp extraction kit (Sigma-Aldrich) and used as a template for PCR ...
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bioRxiv - Microbiology 2023Quote: ... Depending on the culture density up to 1 L of sample was filtered through each (n = 5 per sampling day) 0.22 µm Sterivex polyethersulfone membrane filter unit (Millipore) to obtain biomass for DNA extraction and following amplicon sequencing of 16S and 18S small subunit ribosomal RNA (SSU rRNA ...
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bioRxiv - Neuroscience 2023Quote: ... The antennae were bent outwards from the head and stabilized by a small droplet of n-eicosane (Sigma Aldrich) onto the edge of a microscopy glass slide ...
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bioRxiv - Biochemistry 2023Quote: ... Tubulin was further purified by recycling and some tubulin was labeled with Alexa647-N-hydroxysuccinimide ester (NHS; Sigma-Aldrich), Atto647-NHS (Sigma-Aldrich) ...
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bioRxiv - Physiology 2024Quote: ... Sections were incubated o/n (4°C) with primary antibody against Laminin (Rabbit polyclonal, Sigma, L9393, diluted 1:20). Alexa Fluor Donkey anti-Rabbit 488 (A11034 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-N protein (1:1000, CiteAb #40588-T62) and mouse anti-acetylated α-tubulin (1:200, Sigma-Aldrich #T7451). The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500 ...