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Citations for Millipore Sigma :
9201 - 9250 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... mice were intranasally administered with 3 µg of the RSP protein and 5 μg of cholera toxin (CT; Sigma-Aldrich) as immunological adjuvant ...
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bioRxiv - Bioengineering 2024Quote: ... or 3-5×105 pelleted cells were lysed in TRI Reagent (“LS” for fluid EV samples, conventional for cells, Merck Millipore). Liquid chloroform was added to the lysates at a 1:5 v/v ratio and vigorously mixed for 30 s ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were suspended and incubated for 3 min in 350 µl PBS mixed with 150 µl 0.4% trypan blue solution (Millipore Sigma). For determining viability ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were anesthetized with 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) and mounted laterally in 2% low-melting temperature agarose (Thermo Fisher Scientific 16520) ...
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bioRxiv - Cell Biology 2024Quote: ... 3 days post-fertilization (dpf) larvae were anesthetized in E3 medium containing 0.2 mg/mL Tricaine (ethyl 3-aminobenzoate; Sigma-Aldrich) and maintained at 28.5 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was injected into the fourth ventricle at a final concentration of 1-3 µg/µl in addition to trace amounts of fast-green dye (Sigma). Three 50ms square waveform electrical pulses at 5V (E2 ...
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bioRxiv - Neuroscience 2024Quote: ... Anatomical images were acquired from fish anesthetized with 0.2 mg/ml ethyl-3-aminobenzoic acid ethyl ester (MESAB, catalog # E10521, Sigma-Aldrich). To activate TRPV1-expressing Purkinje cells ...
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bioRxiv - Neuroscience 2024Quote: Larvae were anesthetized in 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) prior to imaging ...
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bioRxiv - Neuroscience 2024Quote: ... coverslips were incubated in 300 μL of primary antibody (anti-TDP-43 1:750, PTGLabs; anti-SUMO2/3 [8A2] 1:500 Sigma) diluted in blocking buffer (1.5% Triton X-100 ...
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bioRxiv - Plant Biology 2024Quote: ... spores were sown directly on plates supplemented with Indole-3-acetic acid (IAA; Alfa aeser) or 1-Naphthaleneacetic Acid (NAA, Sigma). Size measurements were done when plants reached sexual maturity (±6/7 days) ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was extracted from the central body cells of 3-week-old WT and Col5a1+/− menisci by homogenizing freshly dissected tissues in TRI-reagent (T9424, Sigma) and phase-separated in 1-bromo-3-chloropropane (B9673 ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then washed in 1x PBS for 3×30 minutes at RT and mounted using Fluoromount aqueous mounting medium (Sigma).
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bioRxiv - Cancer Biology 2024Quote: ... each coverslip was washed with PBS 3 times each for 5 minutes and nuclei were stained with Hoechst (Merck Sigma, Burlington ...
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bioRxiv - Biophysics 2024Quote: ... The PDMS scaffold surface was activated by oxygen plasma and then treated with a 5% (3-aminopropyl) triethoxysilane (Sigma-Aldrich) solution in methanol for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... After the last EtOH wash the coverslips were incubated with methanol containing 5% acetic acid and 1% (3-Aminopropyl)triethoxysilane (Millipore-Sigma) overnight in a vacuum desiccation chamber in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then collected by centrifugation and washed with 3% FBS in PBS followed by PI (Millipore Sigma, catalog P4170) staining (25 μg/mL PI ...
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bioRxiv - Cancer Biology 2024Quote: HSJD-DIPG007 neurospheres were collected by centrifugation at 800 rpm for 3 min and dissociated into single cells with 1 mL Accutase (Sigma) for 5-10 minutes with occasional pipetting ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% Triton X-100 (PBST)) for 10 minutes and blocked for 1 hour with 10% normal donkey serum (Merck Millipore), 1% bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then washed x3 in 0.24M phosphate buffer solution (PB)(Table 3) and left overnight in 0.12PB with 15% Sucrose (Sigma, 84100-1KG) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Each extract was resuspended in 170 µL of 100% methanol containing isotopically labeled internal standards (5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 1 ug/mL 2-amino-3-bromo-5-methylbenzoic acid, ABMBA, #R435902, Sigma) and centrifuge-filtered (0.22 µm hydrophilic PVDF membrane ...
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bioRxiv - Developmental Biology 2024Quote: Cardiac cells were fixed with cold 4% paraformaldehyde (PFA) solution for 20 min at RT and then washed three times in PBS and once in 3% BSA (Sigma) in PBS for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 1 × 107 CFU were added to a flow cytometry tube containing 1 mL buffer with 10 μM DiOC2(3) (Sigma) and incubated in the dark for 30 min at 30 0C ...
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bioRxiv - Biochemistry 2024Quote: ... were homogenized using a Polytron in 1 ml of 20 mM sodium acetate buffer containing 0.15 M NaCl and 1% Zwittergent 3-14 detergent (Millipore-Sigma 693017), pH 4.75 ...
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bioRxiv - Bioengineering 2024Quote: ... and polyIC incubation was evaluated using the MTT (3-(4, 5dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). The cells were seeded into 96 well-format cell culture plates at a seeding density of 8ξ103 cells per well ...
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bioRxiv - Neuroscience 2024Quote: ... and the samples were washed 3 times for 5 min in PBS and counter-stained with 1:1000 DAPI (D9542, Sigma) for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... 10 min) and resuspended in 3-5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5xCellLytic B [Sigma-Aldrich] ...
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bioRxiv - Immunology 2024Quote: ... followed by activation with 50 mM 3-Dimethylamino-propyl)-ethyl-carbodiimide Hydrochloride (EDC)/100 mM N-Hydroxysuccinimide (NHS) (Sigma-Aldrich) for 12 min at 10 ml/min ...
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bioRxiv - Microbiology 2024Quote: ... bacterial cultures in the exponential phase were collected and then thoroughly mixed with 3 wt% (w/v) of sodium alginate (Sigma) solution to a final concentration of 2.5% alginate ...
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bioRxiv - Immunology 2024Quote: ... Non-specific binding sites were blocked using 300 µl/well of 3% (w/v) skim milk powder (Sigma-Aldrich, USA) in PBS-T ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated for 3 hours with 50 ng/ml phorbol myristic acid (PMA; Sigma-Aldrich, St Louis, MO, USA), 1 μg/ml ionomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... infected cells at different time-points were washed 3 times in 1xPBS and lysed with 0.1% Triton X-100 (#X100 Sigma-Aldrich) in H2O ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed 3 times in 0.01% PBS-Tx (1xPBS with 0.01% Triton X-100 (Sigma-Aldrich, Cat#X100)) for 10 mins at RT ...
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bioRxiv - Biochemistry 2024Quote: ... the enzyme was incubated with 100 μM KCN or 500 μM 3-amino-1,2,4-triazole (ATZ, Sigma, Cat# A-8056) for 30 minutes at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... the column was washed using 50 mL of 10 mM Tris buffer (pH 7.4) containing 100 mM NaCl and 3 % Empigen BB (Sigma-Aldrich) to replace urea and SDS with Empigen ...
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... Filled electrodes then had their tips rapidly dipped in a solution of poly-vinyl chloride in tetrahydrofuran (10 mg in 3 mL; Sigma) to prevent displacement of the ionophore ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... anti-total-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor (anti-tAMPAR) (Cat.#AB1504, Merck Millipore, Burlington, MA, USA), anti-phospho (Ser845)-AMPAR (anti-pAMPAR;cat.#AB5849 ...
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bioRxiv - Plant Biology 2024Quote: Arabidopsis Ws-2 CCR2::LUC WT seeds were surface sterilized and plated on 1x MS medium containing 3% w/v sucrose (Sigma), 1.5% w/v phytoagar (Duchefa) ...
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bioRxiv - Cell Biology 2024Quote: ... Complete depletion of RAD2l in these genome edited cells was achieved by incubation with Inole-3-acetic acid (IAA, ISl48, Sigma) for at least l.S hours ...
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bioRxiv - Cell Biology 2024Quote: ... The vector control and PTP1B knockdown clones were generated in LCN2.3 by transfecting these cells with an shRNA construct against PTP1B and maintained in 0.5μg/ml puromycin (Sigma Catalog #8833) ETS1 knockdowns were performed as described (Chaudhary et al. ...
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bioRxiv - Genetics 2024Quote: ... Then cells were washed with cold staining buffer to terminate reaction and incubated with rabbit complement sera (1:3 dilution, Cat. no. S7764, Sigma) for 30 min at RT ...
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bioRxiv - Genetics 2024Quote: ... and 3-5 µl plasmid solution (Plasmid concentration 100-200 ng/µl) containing Phenol Red for visualization (0.1% Sigma #P0290) was injected intramuscularly using a Nanofil syringe (WPI ...
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bioRxiv - Microbiology 2024Quote: ... RNA extraction was performed according to the manufacturer’s protocol except that 1 M 1-Bromo-3-ChloroPropane (BCP) (Sigma-Aldrich) was used instead of chloroform ...
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bioRxiv - Microbiology 2024Quote: ... Indole-3-carboxaldehyde (I3A, catalog # 129445) and 6-Formylindolo[3,2-b]carbazole (FICZ, catalog # SML1489) were purchased from Millipore Sigma and reconstituted in DMSO ...
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bioRxiv - Genomics 2024Quote: ... followed by TBST washes (3 x 5 min) and then counterstaining with DAPI (20µg/ml in TBST, D9542, Sigma Aldrich). Slides were then mounted using an antifade reagent composed of 10% v/v Mowiol 4 to 88 ...
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bioRxiv - Cell Biology 2024Quote: ... Fully grown oocytes were collected 48 hours after injection and placed in M2 medium containing 200 μM 3-isobutyl-1-methyl-xanthine (IBMX, Sigma) at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... A portion of the LV and septum was minced in a beaker on ice for 3 minutes and subsequently was homogenized using a glass dounce homogenizer at 4°C for 3 minutes in 10 mL of isolation buffer (200 mM Mannitol [M9647, Sigma], 60 mM sucrose [S7903, Sigma] ...
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bioRxiv - Cell Biology 2024Quote: ... A portion of the LV and septum was minced in a beaker on ice for 3 minutes and subsequently was homogenized using a glass dounce homogenizer at 4°C for 3 minutes in 10 mL of isolation buffer (200 mM Mannitol [M9647, Sigma] ...
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bioRxiv - Cell Biology 2024Quote: ... Arteries were then stimulated with increasing concentrations of serotonin (5-HT, from 10–8 to 3·10–5 M, Sigma), endothelium-dependent vasodilator acetylcholine (ACh ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were cultivated under humidified conditions with 5% CO2 at 37°C and passaged every 3-4 days using 0.05% trypsin/EDTA (Sigma-Aldrich). Cells were harvested in ice-cold PBS buffer using a cell scraper ...
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bioRxiv - Neuroscience 2024Quote: ... Two length-matched siblings were anesthetized in 0.2 mg/ml ethyl-3-aminobenzoic acid ethyl ester (MESAB, catalog # E10521, Sigma-Aldrich) simultaneously and mounted in 2% low-melting temperature agar ...