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Citations for Millipore Sigma :
9151 - 9200 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... otherwise as described above) and front-filled by suction with oil containing Na+ ionophores (sodium ionophore II – cocktail A Selectophore™, Sigma Aldrich, UK). An Axon 900B amplifier (Molecular Devices ...
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bioRxiv - Immunology 2022Quote: ... then digested for 2 hours at 37 degrees celsius in a solution consisting of RPMI supplemented with 10% FBS and 0.1mg/mL collagenase II (Sigma Aldrich; St. Louis, MO). After digestion ...
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bioRxiv - Physiology 2022Quote: ... Alzet osmotic minipumps (Model 2004; ALZA Scientific Products, Cupertino, California, USA) were filled with Ang II (Sigma Chemical Co., St. Louis, Missouri, USA) that subcutaneously delivered 1,000 ng/min/kg of Ang II for 28 days ...
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bioRxiv - Neuroscience 2022Quote: ... Hydrogel was prepared separately as solution I and II: Solution I was prepared by dissolving 8 g (16% (wt/vol)) paraformaldehyde (Sigma-Aldrich, Steinheim, Germany) (PFA ...
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bioRxiv - Biochemistry 2024Quote: ... Each reaction mixture constituted protein at the desired concentration with 1.25 mM Tris (2,2’-bipyridyl)dichlororuthenium(II)hexahydrate (Sigma, CAS No. 50525-27-4, and 2.5 mM ammonium persulfate (Sigma, CAS No. 7727-54-0) to a final volume of 10 μL ...
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bioRxiv - Developmental Biology 2023Quote: ... oocytes are first isolated from ovaries by short enzymatic digestion (3 mg/ml Collagenase I, Sigma-Aldrich, #C0130; 3 mg/ml Collagenase II, Sigma-Aldrich, #C6885; 1.6 mg/ml Hyaluronidase, in HL-15, Sigma-Aldrich, #H4272), at 28°C and in HL-15 ...
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bioRxiv - Microbiology 2024Quote: ... instead of 0.5% AlbuMAX II and 30 μM each of the two essential fatty acids oleic and palmitic acid (Sigma #O1008 and #P0500, respectively). After completion of the invasion cycle ...
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bioRxiv - Molecular Biology 2019Quote: ... Myofibers present in a muscle section were stained with an anti-myosin antibody (MF 20, DSHB) while anti-laminin antibody (Sigma) was used to visualize the outline of all myofibers present in a given muscle section ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were loaded and run on SDS-PAGE gels, transferred to PVDF membranes, and immunoblotted with antiKv2.1 antibody (K89/34, NeuroMab) and anti-α tubulin antibody (Sigma). The blots were detected using chemiluminescence reagent on a Bio-Rad Chemidoc MP and Image J was used for quantification of detected bands.
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bioRxiv - Molecular Biology 2020Quote: ... antibodies, and the secondary antibodies used included anti-rabbit (31460, Pierce), anti-mouse (31430, Pierce) and anti-rat (A9037, Sigma) HPR antibodies ...
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bioRxiv - Biochemistry 2019Quote: ... anti-actin (A4700) and anti-tubulin antibody (T6199) and goat anti-human IgG–HRP antibodies were obtained from Sigma-Aldrich, Dorset ...
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bioRxiv - Genetics 2021Quote: ... 10 μg of chromatin was immunoprecipitated with the one of the following antibodies: 5 μg CTCF antibody (07-729, Merck-Millipore), 5 μg RAD21 antibody (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... Pierce anti-HA beads for immunoprecipitation of HA-tagged GAPDH was purchased from Thermo Fisher (Cat. # 88836) Rabbit polyclonal antibody (Cat. # G4595) and mouse monoclonal antibody against GAPDH (Cat. # G8795) were purchased from Sigma Aldrich. Rabbit polyclonal antibody against Peroxiredoxin-SO3 (Cat ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-α-tubulin primary antibody as well as secondary antibodies coupled to horseradish peroxidase (HRP) were obtained from Sigma-Aldrich. AlexaFluor-labelled secondary antibodies were obtained from ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation reactions were performed using anti-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, F1804, Sigma-Aldrich). The complex of chromatin antibody was captured with protein G beads (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblot of phosphorylated MAPKs was performed using anti-phospho p44/42 MAPK antibody as the primary antibody and peroxidase-conjugated goat anti-rabbit IgG (Sigma) as the secondary antibody.
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Microbiology 2021Quote: ... We just changed the secondary antibody and for this step horseradish peroxidase (HRP)-conjugated rabbit anti-bovine IgG antibody (Sigma) was used ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Flag (M2) and anti-MAP2 (HM-2) antibodies as well as polyclonal rabbit anti-Flag antibodies (F3165, F7425) were from Sigma. Anti-Xpress antibodies were from Invitrogen (46-0528).
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with an alkaline phosphatase-conjugated secondary antibody directed against the first antibody (Sigma Aldrich, Taufkirchen, Germany) for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Cell Biology 2022Quote: The mouse monoclonal anti-GABAA α1 subunit antibody (clone BD24) (catalog #: MAB339) and mouse monoclonal anti-GABAA β2/β3 antibody (catalog #: 05-474) were obtained from Millipore. The rabbit monoclonal anti-GABAA α1 subunit antibody (catalog # ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti-β-actin antibody (catalog #: A1978) and mouse monoclonal FLAG antibody (catalog #: F1804) came from Sigma Aldrich. The fluorescent anti-β-actin antibody Rhodamine came from Biorad (catalog # ...
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bioRxiv - Microbiology 2020Quote: ... In this case the blot was incubated with the IcdH primary antibody at room temperature for 2 hours and with the secondary antibody (anti-rabbit HRP, Sigma) was done at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Blotting was performed according to standard procedures using primary antibodies as follows: Two Lpd-specific antibodies were purchased from Sigma (HPA016744 recognising the N-terminus ...
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bioRxiv - Microbiology 2021Quote: ... except that the primary antibody for viral detection was a monoclonal antibody against the coronavirus group antigen (Milipore Sigma #MAB9013) and the secondary was a horseradish-peroxidase-conjugated goat-anti-mouse antibody (enQuire BioReagents # Q2AB1).
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Biochemistry 2019Quote: ... the presence of the viruses was revealed by immunostaining in infected cells with a polyclonal rabbit antibody that recognizes the MOPV Z protein (Agrobio, France) and a phosphatase alkaline-conjugated polyclonal goat anti-rabbit antibody (Sigma) and 1-Step NBT/BCIP substrate (Thermo Fisher scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... similarly performed as in co-immunoprecipitation except that the primary antibody used is anti-PCNA antibody (cat#SAB2108448; Sigma-Aldrich) in 1:750 dilutions.
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bioRxiv - Cell Biology 2019Quote: ... gels were transferred onto 0.45 μm PVDF membranes using a semidry transfer apparatus before being subjected to immunoblot analysis using specific primary antibodies and secondary antibodies (Sigma). Protein detection was performed using ECL chemiluminescent reagent (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... Bound primary antibodies were detected using peroxidase-coupled secondary anti-rabbit antibody (7074, Cell signalling) and enhanced chemiluminescence (WBLUF0500, Millipore). Blots were exposed digitally using the ChemiDoc MP System (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... washed three times with TBST and incubated with the primary antibody – the polyclonal anti-Dam1p (Keating et al., 2009) or monoclonal anti-GFP antibody (#11814460001 from Sigma) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: Isotyping of the monoclonal antibodies and polyclonal sera responses was performed using the Mouse Monoclonal Antibody Isotyping Kit (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The following primary antibodies were used for the Western blotting: mouse antibodies against Flag (Sigma-Aldrich, St. Louis, MO, USA) and mouse antibody against β-actin (Proteintech Group ...
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bioRxiv - Cell Biology 2020Quote: ... The target protein was detected by specific primary antibodies followed by secondary horseradish peroxidase (HRP)-conjugated antibodies (for less abundant proteins) (Sigma) or fluorescence-labeled secondary antibodies (for abundant antigens ...
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bioRxiv - Immunology 2020Quote: ... Immunofluorescence staining using a goat anti-LYVE1 antibody (R&D) followed by incubation with an Alexa647-conjugated secondary antibody together with Hoechst33342 (Sigma) was performed on 7 µm sections without acetone fixation ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated with primary antibodies specific for TSC22D4 (home-made, by PINEDA Antibody Service, Flag-HRP (#A8592, Sigma), HA (#2999 ...
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bioRxiv - Cancer Biology 2020Quote: ... serum samples used to detect levels of IgG by anti-IgG-HRP antibody (Bio-Techne) or IgM by anti-IgM-HRP antibody (Sigma) on 100μg/ml NP-KLH-coated 57 ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... RPN11- 3xFLAG tagged subunits were detected by monoclonal anti-FLAG antibodies (Monoclonal ANTI- FLAG® M2 antibody #F3165, Millipore Sigma). The lysate from a wild-type strain with untagged RPN11 was used as a negative control ...
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bioRxiv - Genomics 2022Quote: ... Infectious foci were stained with a human anti-SARS-CoV-2 Spike antibody (H2-162, Hugo Mouquet’s laboratory, Institut Pasteur) and the corresponding HRP-conjugated secondary antibody (Sigma-Aldrich). Foci were visualized by 3,3’-diaminobenzidine staining solution (DAB ...
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bioRxiv - Microbiology 2022Quote: ... samples were washed with 1 ml PBS buffer three times and vacuum-infiltrated for 20 min with 0.1 mg/ml of primary mouse anti-1,3-β-glucan antibody (Biosupplies; Sydney, Australia) for β-1,3-glucan labelling or 0.1 mg/ml mouse MOPC-104E primary antibody (Sigma-Aldrich) for α-1,3-glucan labelling ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Secondary antibody HRP conjugated-rabbit anti-goat IgG (cat#: AD106P) and primary anti-MPO antibody (cat#: AF3667) were purchased from Sigma and R&D systems ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS twice and then incubated in antibody solution (2% NGS, 0.2% Triton-X-100, PBS) with anti-PSD-95 antibody (Millipore, MABN68) for overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed for target proteins with specific antibodies against phosphorylated or total proteins and secondary antibodies before being developed using Immobilon chemiluminescent substrate (Millipore) and visualized using the Odyssey Infrared Imaging System (LI-COR).
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bioRxiv - Cancer Biology 2024Quote: ... About 3x106 cells were immunoprecipitated using 10 µg of a monoclonal SF3B1 antibody (clone 16, MBL D221-3) or 10 µg of the monoclonal U2AF2 antibody (U4759, SIGMA).
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bioRxiv - Bioengineering 2024Quote: ... MyoD (primary Anti-MyoD Antibody, #ZRB1452, Sigma-Aldrich, and an anti-rabbit secondary antibody coupled with Rhodamine #SAB3700846, Sigma-Aldrich), DAPI (4′-6-diamidino-2-phenylindol ...
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bioRxiv - Neuroscience 2024Quote: Detection of newly synthesized proteins by proximity ligation assay was carried out using anti-puromycin antibody in combination with protein-specific antibodies and detection using Duolink reagents (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody is diluted in blocking buffer and slides are incubated in primary antibody overnight at 4°C (ChAT, Millipore, AB144P ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunolabeling with a rat anti-phospho- histone H3 antibody (polyclonal rat antibody against phosphorylated Ser28 histone H3, Sigma- Aldrich, H9908) was carried out following the previously outlined techniques ...
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bioRxiv - Microbiology 2024Quote: ... and MreB or Spo0J were detected using respective in-house polyclonal antibodies and HRP-conjugated anti-rabbit IgG antibody (Sigma). Samples were developed using Clarity Western ECL Substrate (Bio-Rad ...