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Citations for Millipore Sigma :
9101 - 9150 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were incubated with a monoclonal anti-myc antibody (Covance, MMS-150R) or anti-Flag antibody (Sigma, F3165) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... were performed prior to primary antibody incubation (12hrs, biotinylated mouse monoclonal antibody, Sigma-Aldrich SIG-39240 Beta-Amyloid - 4G8) at 2μg/ml (antibody diluent ...
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bioRxiv - Cancer Biology 2022Quote: ... which were subsequently incubated overnight at 4 °C with primary antibodies as follows: anti-P2X7 antibody (P8232, Sigma-Aldrich) was diluted 1:300 ...
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bioRxiv - Molecular Biology 2022Quote: ... for 30 min at room temperature and incubated with the following primary antibodies diluted in Antibody Diluent (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Antibodies used for the western blots were obtained from commercial suppliers: anti-actin monoclonal antibody (Sigma-Aldrich A0480-200μL) and anti-mouse antibody (Agrisera AS111772) ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were immunoprecipitated with ACC antibody (CST) or ACC2 antibody (CST) and EZview Red Protein G Affinity Gel (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used for experiments in this work: polyglutamylated tubulin (mouse monoclonal antibody, clone B3 T9822, Sigma), pan-polyglycylated antibody (mouse monoclonal antibody ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we used both anti-α-tubulin antibody and antibody against FMRFamide (rabbit polyclonal; Millipore, Sigma, Cat# AB15348, RRID: AB_805291). After incubating in a blocking solution of 6% goat serum in PBS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we used both anti-α-tubulin antibody and antibody against FMRFamide (rabbit polyclonal; Millipore, Sigma, Cat# AB15348, RRID: AB_805291). After incubating in a blocking solution of 6% goat serum in PBS ...
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bioRxiv - Immunology 2021Quote: ... The bound antibodies were revealed using a HRP-conjugated goat antibody to mouse Ig absorbed with human Ig (Sigma) (1:4,000 dilution) ...
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bioRxiv - Microbiology 2021Quote: ... The following primary antibodies were used: monoclonal rabbit antibody against COX-2 (Clone SP21, Cat# SAB5500087-100UL, Sigma-Aldrich), mouse monoclonal anti-acetylated α-Tubulin antibody (Cat# sc-23950 ...
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bioRxiv - Plant Biology 2022Quote: ... and αTubulin levels were used as loading control (α_Tubulin antibody, Monoclonal Anti-α-Tubulin antibody produced in mouse, Sigma, Catalog number T5168 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in primary antibody solution of rabbit polyclonal antibody to tyrosine hydroxylase (TH; Sigma AB152, RRID: AB_390204) in 5% normal donkey serum in TBS at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Molecular Biology 2023Quote: ... an anti-GFP polyclonal antibody (Medical & Biological Laboratories) and a rabbit horseradish-peroxidase-linked IgG whole antibody (Sigma-Aldrich) were used as the primary and secondary antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... anti-FLAG antibody conjugated with horse radish peroxidase (A8592,1:10000) and HA antibody (SAB4300603,1:1000) were purchased from Sigma-Aldrich. Antibody against β-actin (sc-4777 ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound primary antibodies were incubated with HRP-conjugated secondary antibodies (Amersham) and detected using chemiluminescence (Luminata HRP substrate, Millipore). List of antibodies used is listed in Supplementary Information 1.
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bioRxiv - Molecular Biology 2024Quote: ... The membranes probed with the appropriate primary antibodies were incubated with secondary antibodies linked to horseradish peroxidase (HRP) (Sigma). Blots were revealed using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The beads were separated on a magnet and resuspended in 25 µl of cold Antibody buffer (Wash buffer with 0.1% BSA) containing respective primary antibody or IgG (TFE3, Sigma HPA023881 ...
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bioRxiv - Physiology 2023Quote: ... Mouse monoclonal anti-FLAG M2 antibody (F1804) and mouse monoclonal anti-β-actin antibody (A1978) were obtained from Sigma. HRP-conjugated goat anti-rabbit (CST#7074 ...
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bioRxiv - Cell Biology 2023Quote: ... or NK92 cells with antibodies against acetylated (Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse, Sigma-Aldrich, Cat# T6793) and detyrosinated (Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker ...
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bioRxiv - Cell Biology 2023Quote: Other primary antibodies used were: Atlas Antibody anti-MYO18A (Cat# HPA021121, RRID:AB_1854250) from Sigma-Aldrich (St. Louis, MO, USA); Proteintech (Rosemont ...
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bioRxiv - Molecular Biology 2023Quote: The following primary antibodies were used in this study: anti-v-Src (OP07) antibody was purchased from Sigma-Aldrich. Anti-phospho-Src family (pY416 ...
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bioRxiv - Pathology 2023Quote: ... Primary antibodies were incubated overnight at room temperature and diluted in antibody diluent (Sigma Aldrich, St. Louis, MO, USA) as follows ...
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bioRxiv - Cell Biology 2022Quote: ... They were then blocked in 10% goat serum and incubated with primary antibodies (anti-phosphotyrosine antibody (4G10; EMD Millipore), 1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody, A3682, Sigma Aldrich). All three blots were developed for 9.2 s after adding SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse and anti-rabbit IgG horseradish peroxidase (HRP)-linked antibodies (Amersham) and streptavidin HRP-conjugated antibodies (Sigma-Aldrich) were used for ECL detection Reagents (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were re-suspended in antibody buffer (50 μL) with BG4 (∼5 μM, 4 μL/sample) or H3K27ac antibody (0.5 μL/sample, Millipore) and incubated at 4 ◦C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... primary antibodies were incubated overnight at 4 °C and secondary antibodies with 0.2 mg/ml bisBenzimide Hoechst (Sigma-Aldrich) or 1 µg/ml DAPI (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... detached leaves were incubated for 30 min in lactophenol Trypan Blue solution (water: glycerol: lactic acid: phenol [1:1:1:1] + Trypan Blue solution [1 mg mL-1; Sigma-Aldrich]). Thereafter ...
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bioRxiv - Molecular Biology 2021Quote: ... were prepared by extraction with trichloroacetic acid (TCA) and subjected to Western blot analysis as described previously (13) using antibody against the His6 epitope (EMD Millipore/Novagen 70796, 1:2000 dilution) Experiments were repeated three or more times from Biological replicates.
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bioRxiv - Developmental Biology 2020Quote: ... in TNT for 2-3 h at room temperature and incubated with an anti-Fluo-POD antibody (1/50, Sigma-Aldrich REF 11 426 346 910) in 2% TNB overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... For visualization of the eya1 probe the samples were incubated with an anti-Dig-POD antibody (1/50 in 2% TNB, Sigma-Aldrich REF 11 207 733 910) and DAPI (1/1000 ...
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bioRxiv - Biophysics 2019Quote: ... The membrane was rinsed three times in TBS-Tw and incubated for 30 min with primary antibody (M2 anti-FLAG from Sigma, diluted 1:500 in TBS-Tw). The membrane was rinsed three more times in TBS-Tw and incubated for 30 min with secondary antibody (HRP-conjugated sheep anti-mouse IgG ...
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bioRxiv - Neuroscience 2021Quote: ... and immunoblotted overnight at 4°C with shaking in Intercept Blocking Buffer with the following antibodies: α GluA2 (1 µg/mL; Millipore, MAB397 or Alomone, AGC-005); α GluA1 (1 µg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto a nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Microbiology 2019Quote: ... The membrane was washed five times with TBS-T and incubated for 1 h at room temperature with the anti-IgY secondary antibody conjugated to peroxidase (Sigma-Aldrich®, St. Louis, MO, USA), diluted 1:1,000 ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins of the eluate were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Biochemistry 2019Quote: ... secondary antibody incubation was carried out for 1 h at RT followed by staining of the nuclei with DAPI (Sigma, Cat. No. 32670, Lot No. BCBD9022V) and mounting of the sections with ProLong Gold Antifade Mountant (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were blocked in 5% FBS then incubated overnight at 4 C with primary mouse antibody against HA (H9658, Sigma-Aldrich, Oakville, ON, Canada, 1:1000) and then with secondary Alexa 488 antibody against mouse (Invitrogen ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 anti-RBD and N IgG antibody levels were determined using the Milliplex® SARS-CoV-2 antigen panel 1 IgG assay (Millipore Sigma, Cat No. HC19SERG1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4 °C in the following primary antibodies diluted in TBST: 1:200 dilution of anti-CRABP2 (MAB5488, EMD Millipore/Sigma Aldrich, St. Louis MO), 1:200 dilution of anti-RALDH2 (#13951-1-AP ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10% vol/vol foetal bovine serum (Hyclone FetalClone II Serum, Fischer Scientific, New Hampshire, US) and 21% vol/vol F12 nutrient mix (Sigma-Aldrich, Gillingham, UK), supplemented with 2 mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Primary osteoblast precursors were isolated from the calvarias of newborn mice by sequential digestion (0.2 % dispase Grade II (Roche, Sigma-Aldrich, /collagenase Typ Ia, Sigma Aldrich, C-9891). Cells were seeded (2×104 cells/well ...
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bioRxiv - Immunology 2021Quote: ... Minced heart tissue was digested in 3 mL of digestion buffer (2 mg/mL Collagenase IV [Worthington Biochemical], 1.2 units/mL Dispase II [Sigma-Aldrich], in perfusion buffer) for ∼45 minutes at 37° C using a rotating holder ...
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bioRxiv - Cell Biology 2021Quote: ... and the bone marrow cells were flushed out before the long bones were chopped and digested by collagenase II (Sigma, 0.1% vol/vol) at 37 °C for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were minced into small pieces with a razor blade and digested with Dispase II (1.25 units/ml, D4693, Sigma-Aldrich, St. Louis, MO), 0.4 mg/ml collagenase IV (C5138 ...
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bioRxiv - Neuroscience 2021Quote: ... For FACS sorting a cell suspension was obtained upon digesting the tissue in 2 mg/ml Dispase II (D4693, Sigma-Aldrich, Steinheim, Germany), 2 mg/ml Collagenase IV (#1710401 ...
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bioRxiv - Cancer Biology 2021Quote: ... glucose metabolic inhibitor 2DG (10 mM or 20 mM) was added 15 min before cell lysis and ATP determination using the ATP Bioluminescence Assay Kit HS II (Sigma catalogue number 11699709001) and analyzed on an Infinite M200 PRO Luminometer (Tecan).
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bioRxiv - Neuroscience 2021Quote: ... further dissociated with enzymatic solution (0,01% Papain, Sigma, 10108014001; 0,1% dispase II, Sigma, D4693; 0,005% DNAseI, Sigma, 10104159001; 12,4 mM MgSO4 in HBSS) for 20 min at 37 °C and filtered through 70 µm nylon mesh (Miltenyi ...