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Citations for Millipore Sigma :
9151 - 9200 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... CRY1 proteins were purified using Myc resin (EZview™ Red Anti-c-Myc Affinity Gel, SIGMA-ALDRICH Catalog No: E6654) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: Protein half-life was measured by treating cells with DMSO as control or 100 μg/ml of cycloheximide (Sigma, #C4859) for 30 or 60 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation at 500 g for 10 minutes and filtration through nylon low protein-binding filters (SLHP033RS, Millipore). Virus was concentrated by ultracentrifugation (employing a SW32 rotor at 25K for 2hr and 30min at 4° C ...
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bioRxiv - Genetics 2021Quote: ... the resin was washed 5 times with ice cold DOC buffer and the bound protein eluted by 15 minute 70°C incubation in 5% w/v sodium dodecyl sulphate (SDS, Sigma). The eluted protein was reduced with 10 mM TCEP and alkylated using 20 mM Iodoacetamide ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (DE3) (Transgen, CD601-02) and protein expression was induced by an auto-induction system (71757-5, EMD Millipore). Upon overnight induction at 25°C ...
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bioRxiv - Microbiology 2021Quote: ... After blocking samples were incubated for 120 hours with primary antibody or trimeric RBD protein in PBSGT with 0.1% saponin (S2149, Sigma-Aldrich) on a shaking incubator at 200 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were separated by SDS-PAGE under reducing conditions and transferred to polyvinyl difluoride (PVDF) membranes (Millipore, Billerica, MA). Antibody incubations were performed in 3% BSA in TBS buffer using the following antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
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bioRxiv - Molecular Biology 2020Quote: ... then 1-2 mg (as indicated) of total protein was incubated with pre-washed M2 α-FLAG magnetic beads (Sigma) or MagStrep “type3” XT beads (IBA ...
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bioRxiv - Cell Biology 2021Quote: ... protein was dialyzed into NMR buffer [30 mM phosphate buffer pH 7.4 (22.6 mM Na2HPO4 (Sigma-Aldrich, Cat. No. S9763) and 7.4 mM NaH2PO4 (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: The coding sequence of Hd3a and RFT1 were cloned into the pCold-GST vector42 and produced as a glutathione S-transferase (GST) fusion protein in Escherichia coli BL21 Rosetta (DE3) (Novagen). Cells were grown in LB medium or minimal medium containing 0.5 g l™1 of 15N-ammonium chloride ...
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bioRxiv - Plant Biology 2021Quote: ... and 1 × protease inhibitor cocktail] and precleared by incubation with 50 μL Protein-A agarose beads/Salmon sperm DNA (Millipore) at 4 °C for 1 h ...
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bioRxiv - Plant Biology 2021Quote: ... and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000, Sigma) and anti-MAPK6 (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... and the electrophoresed proteins were then transferred onto a polyvinylidene difluoride membrane (Immobilon®-PSQ membrane, Millipore GmbH, Eschborn, Germany) for immunoblotting analysis ...
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bioRxiv - Neuroscience 2021Quote: ... 200µl of total protein was further fractionated into soluble and aggregated forms using membrane based centrifugal filter unit (Millipore MRCF0R100) 500X g for 15 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The debris were removed from the lysates by centrifugation and protein estimation was performed by Bicinchoninic Acid (BCA) assay (Sigma). Proteins were resolved by loading 10ug of protein on a 10% and 12% SDS-PAGE gel ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing equal amounts of protein were separated by SDS-PAGE (4% stacking and 12% resolving gel) and transferred onto a PVDF membrane (Millipore). The membrane was blocked with 5% skim milk (for detection of E ...
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bioRxiv - Neuroscience 2020Quote: ... the coding region corresponding amino acid 321-475 of dHtt protein was amplified by PCR and cloned into pET28b expression vector (Novagen). To generate anti-CG8134 antibody ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were resolved on 8-15% SDS-PAGE depending on protein size and transferred to polyvinylidene difluoride membrane (Immobilon-P, Millipore). Membranes were blocked with PBS-T (PBS ...
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bioRxiv - Microbiology 2021Quote: ... and fractions containing protein of the appropriate size were pooled and concentrated using 10 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Millipore, UFC505024) into buffer without imidazole (20 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... Beads were washed with TBS-lysis buffer and proteins were eluted from beads by addition of 3×Flag peptides (150 μg/mL, Sigma) for 1 h at 4°C and then subjected to tryptic digestion followed by LC-MS/MS ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were first precleared by incubation with 40 µL of protein A-sepharose beads CL-4B (Sigma, #GE17-0963-03) for 1 h at 4°C and then incubated with mouse mAb anti-HA (clone 16B12 ...
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bioRxiv - Microbiology 2021Quote: ... 20 µM of copper-bound proteins were taken in duplicates and incubated with 100-fold excess of the copper chelator bathocuproine disulfonate (BCS) (Sigma) and 100-fold excess of the reducing agent hydroxyl amine (NH2OH ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein fractions were concentrated to ∼12 mL using an Amicon Ultra-50 centrifugal filtration device (100-kDa MW cutoff, Millipore) and loaded onto a HiPrep Desalting column 26/10 (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... The protein was concentrated to 15 mL using an Amicon Ultra-15 centrifugal filtration device (50-kDa MW cutoff, Millipore). The protein was diluted with a modified buffer A (8% (vol∕vol ...
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bioRxiv - Biophysics 2021Quote: ... The fractions of the flow-through containing MSP protein were pooled together and concentrated with a 10 kDa Amicon Ultra-filter (Millipore) to 100-200 μM ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10-30 μg total cellular protein were resolved on SDS-PAGE and transferred onto Immobilon-FL PVDF membranes (Millipore). Bound antibodies were detected with IRDye infrared secondary antibodies using the Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then followed the manufacturer’s protocol to extract soluble proteins by using CelLytic™ B Cell Lysis Reagent (B7435-500ml, Sigma). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were then pre-cleared using 25 μL of Protein A agarose beads (Pierce) and then incubated with 25 μL of mouse anti-V5 agarose beads (Sigma) and washed with RIPA buffer ...
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (Chatterjee et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant GST fusion proteins were eluted by resuspending the resin in elution buffer containing 15 mg/mL reduced L-glutathione (Sigma) and incubated at 4°C for 4 hours ...
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bioRxiv - Microbiology 2022Quote: 50-100 μg of protein extracted as above was incubated with 25 μL anti-FLAG M2 magnetic beads (Sigma-Aldrich) in modified 1X RIPA in a total volume of 300 μL at 4°C overnight with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... the protein was eluted in 10-13 ml elution buffer and concentrated with a centrifuge filter (Amicon Ultracel-30K, Millipore). Thereafter ...
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bioRxiv - Biophysics 2022Quote: ... Protein was buffer exchanged into phosphate buffered saline (PBS) containing 10% glycerol using a spin concentrator (10 kDa MWCO, Millipore).
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bioRxiv - Plant Biology 2022Quote: ... 100 ng extracted protein for each plants were separated via SDS-PAGE and analyzed using anti-actin (Sigma A0480, MFCD00145889) at 1:1000.
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bioRxiv - Plant Biology 2022Quote: ... A 1–month–old ICR mouse (SLAC laboratory animal) was injected with 50 μg GST–CAR9 protein emulsified with Complete Freund’s adjuvant (F5881, Sigma–Aldrich). Two weeks later ...
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bioRxiv - Biochemistry 2019Quote: ... Disulfide bonds were reduced by adding 2mM Dithiothreitol (DTT) (Fisher) and protein alkylation was performed with 10 mM iodoacetamide (IAA) (Sigma). Samples were acidified by adding phosphoric acid to a final concentration of 1.2% and subsequently diluted 7-fold with 90% methanol in 100mM TEAB ...
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bioRxiv - Biochemistry 2019Quote: ... the presence of the viruses was revealed by immunostaining in infected cells with a polyclonal rabbit antibody that recognizes the MOPV Z protein (Agrobio, France) and a phosphatase alkaline-conjugated polyclonal goat anti-rabbit antibody (Sigma) and 1-Step NBT/BCIP substrate (Thermo Fisher scientific ...
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bioRxiv - Cell Biology 2019Quote: ... and His12-Arf1 proteins were expressed as the N-terminal GST-His6-tagged form in Escherichia coli BL21(DE3) cells (Novagen) harboring pET-41-based vectors in Lysogeny Broth (LB ...
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bioRxiv - Neuroscience 2019Quote: Tau variants (full length protein and a fragment encoding amino acids 256-368) were cloned into the pET19b vector (Novagen) in between the NcoI and BamHI restriction sites ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Protein samples were separated by sodium dodecyl sulfate (SDS)-PAGE and transferred onto a nitrocellulose membrane (Millipore, Temecula, CA, USA). After blocking with 5% non-fat powdered milk ...
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bioRxiv - Biochemistry 2019Quote: ... were incubated overnight at 4°C with the chromatin extracts and then coupled with 50 μl of protein-G-sepharose beads (GE17-0618-01, Sigma) during 4h at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Sonicated lysates were pelleted for 5 min at 14000 rpm in a microcentrifuge and the entire supernatant was transferred to a new microfuge tube and incubated overnight at 4°C with 5 µg of anti-Myc antibody (9E10) and 20 µl of protein G magnetic beads (Millipore). Following IP ...
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bioRxiv - Molecular Biology 2019Quote: ... degradation of V5-AID-fused Brn1 protein was induced by addition of 0.5 mM indole-3-acetic acid (Auxin, Sigma # 13750).
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bioRxiv - Cancer Biology 2019Quote: ... Methanol/chloroform extraction was performed on of protein for each lysate and pellet finally re-dissolved in 100ul of 6M Urea (Sigma), 2M thiourea (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... Bound proteins were separated by SDS-PAGE and transferred to nitrocellulose before incubation with an anti-myc antibody (9E10; Sigma). Blots were revealed with the Odyssey Western Detection System.
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bioRxiv - Systems Biology 2019Quote: ... and searched with MaxQuant 1.5.1.228 against the yeast protein database from Ensembl 89 and the UPS spike-in Fasta file obtained from Sigma-Aldrich.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... aliquots of 15 µg of hippocampal protein were extracted with lysis buffer containing phosphatase and protease inhibitors (Cocktail II, Sigma) and protein concentration was determined by Bradford’s method ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were centrifuged at 20,000 × g for 20 min at 4°C and the total protein lysate was quantified using Bradford Reagent (B6916; Sigma). Then ...