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Citations for Millipore Sigma :
9151 - 9200 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... and subsequently used for IP with 48 μg of an anti-NaVPAN mouse monoclonal antibody (mαNaVPAN, Sigma-Aldrich, #S8809), raised against the SP19 epitope (Vassilev et al. ...
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bioRxiv - Microbiology 2022Quote: ... incubated 1 hour with primary mouse monoclonal antibody anti-HA tag (G036, Abm Inc.) diluted 1:10,000 and then probed with horseradish peroxidase-labeled anti-mouse immunoglobulin (A9044, Sigma), diluted 1:15,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were incubated with 15 μg of mouse monoclonal anti-FLAG M2 antibody (Sigma-Aldrich, F1804, RRID: AB_262044) in 1 mL of PBS containing 0.5% BSA and 0.1% Triton X-100 at 4°C for 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Sporozoites were incubated for one hour at room temperature with mouse anti-tubulin (1:500, Sigma-Aldrich, Ref T5168) to stain microtubules followed by three washing steps with RPMI ...
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bioRxiv - Immunology 2022Quote: ... the cells were immunostained with a mouse monoclonal antibody against SARS-CoV-1/2 nucleoprotein [clone 1C7C7 (Sigma-Aldrich)] ...
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bioRxiv - Neuroscience 2022Quote: ... live hippocampal cultures were incubated with an N-terminal APP antibody (mouse anti-APP 22C11, 1:500; EMD Millipore) diluted in conditioned neuronal media for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the brain slices were washed 3 times using 1 x PBS in a shaker and incubated in blocking solution (10% normal goat serum and 0.2% Triton X-100 in PBS) for more than 1.5 hours in a shaker and incubated with the primary antibody (Mouse anti- GAD67, Millipore; Mouse anti-CaMK2a ...
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bioRxiv - Molecular Biology 2022Quote: ... Nanobody fusion proteins were detected using a primary anti-HA (mouse; 1:3,000, Sigma-Aldrich, St. Louis, MO, USA). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were rabbit anti-MAP2 (ab281588, abcom) and mouse anti-β-tubulin III (T8578, Sigma-Aldrich). Then ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed once with TBS pH 8.0 for 5 min and incubated for overnight at 4 °C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Neuroscience 2024Quote: ... Adjacent compartments through the ventral midbrain were processed using mouse anti-CaBP (1: 10,000, C9848, Sigma, St. Louis, MO) and mouse anti-TH (1:10,000 ...
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bioRxiv - Neuroscience 2024Quote: Sox14gfp/+ and Sox14gfp/gfp mouse brain samples were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...
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bioRxiv - Immunology 2024Quote: ... with the test mAb diluted to 5 µg/mL and AP-conjugated goat anti-mouse IgG (A3562 Sigma-Aldrich) used as a secondary.
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bioRxiv - Immunology 2024Quote: ... the cells were incubated in a 1:500 dilution of 4D2 mouse anti-PLSCR1 antibody (Millipore Sigma, cat. #MABS483) in PBS-BG (1x PBS with 0.5% bovine serum albumin and 0.1% glycine ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse zygotes were isolated in FHM under mineral oil and cumulus cells were removed with hyaluronidase (Sigma-Aldrich; H4272). Mouse embryos were cultured in pre-equilibrated Global Media (Cooper Surgical ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Neuroscience 2024Quote: ... to perform immunohistochemical procedures in the Penn Digital Neuropathology Lab as described previously.21,61 Immunohistochemistry for neuronal markers included antigen retrieval using citrate buffer and heat for neuronal nuclei protein (NeuN) enriched in most neurons (Anti-NeuN mouse monoclonal antibody, clone A60,1:1000, Millipore) and heavy non-phosphorylated neurofilament (NF-H ...
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bioRxiv - Cancer Biology 2024Quote: Protein-protein interaction was measured by PLA with Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92102, Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 5 mg of mouse VAT tissue was homogenized in 300 μl of RIPA lysis buffer (R0278-50ML, Sigma-Aldrich) containing protease inhibitor and centrifuged at 12,000g for 10min at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000, Sigma) and anti-c-Myc rabbit antibody clone 7E18 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... spleen cells from a single mouse were harvested and stimulated for 6 h with 16 nM PMA (Sigma-Aldrich) and 1 μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The FLAG-tagged NiV-F and mutants were detected by the anti-FLAG mouse monoclonal antibody (Sigma-Aldrich, F1804) and an Alexa Fluor® 647 conjugated donkey anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... samples stained with primary FBXO11 (mouse) and LONP1 (rabbit) antibodies were incubated with Anti-Rabbit PLUS (Sigma-Aldrich DUO92002) and Anti-Mouse MINUS (Sigma-Aldrich DUO92004 ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibody used was goat anti-mouse IgG (Fc specific) conjugated with alkaline phosphatase from Sigma (CAT#A1418) and used at 1:3000 dilution.
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bioRxiv - Neuroscience 2024Quote: ... each mouse received a bilateral injection of 1.25 μl of 6-hydroxydopamine hydrochloride (6-OHDA, Sigma-Aldrich, 4μg/μl) or vehicle (0.9 % NaCl + Ascorbic Acid 0.02% ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated with the primary antibody solution at 4 °C overnight (mouse anti- FLAG (1:1000; Sigma-Aldrich; F1804), mouse anti-RpS6 (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... were washed 3 x with PBS followed by incubation with 20 μg mouse anti-FLAG M2 Ab (Sigma Aldrich) for 2 h at 4 °C with rotation prior to 3 x PBS washes and resuspension in PBS ...
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bioRxiv - Genomics 2024Quote: ... Samples were incubated with primary antibodies (mouse anti-acetyl-alpha tubulin antibody, clone 6-11B-1 [Millipore-Sigma Aldrich, cat# ...
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bioRxiv - Microbiology 2024Quote: ... Membrane was washed 3 times for 5 minutes with TBS-T and incubated in secondary antibody 1:10,000 dilution goat anti-mouse HRP (Sigma).
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bioRxiv - Immunology 2024Quote: ... nonspecific antibody binding was blocked by incubation with an anti-CD16/32 antibody (clone 2.4G2; UCSF Ab production facility) and whole mouse IgG (Sigma) on ice for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was used for a western-blot experiment using a mouse-anti-Puromycin antibody (1:5,000, Millipore MABE343). To detect primary Ab ...
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bioRxiv - Biochemistry 2024Quote: ... Transferred PVDF membranes were blocked in 5% milk powder in 0.2% Tween-TBS overnight at 4°C and immunoblotted with mouse anti-FLAG-HRP (Sigma) conjugated primary antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... #9664, CST), rabbit anti-GAPDH (1:10,000, #2118, CST) and mouse anti-β-actin (1:10,000, #A5441, Sigma-Aldrich). GAPDH and β-actin served as loading controls.
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bioRxiv - Cell Biology 2024Quote: ... hiPSC-CMs were grown on coverslips and were stained with anti-α-actinin (mouse, Sigma-Aldrich, A7811, 1:300) and anti-Tomm20 (rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in a primary antibody solution containing 1:250 mouse anti-NeuN (Millipore Sigma; MA, USA) or 1:200 goat anti-DCX (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies (insulin rabbit monoclonal, Cell Signaling 3014, 1:3000 and glucagon, mouse monoclonal, Sigma-Aldrich G2654, 1:1000) were applied and incubated for 60 minutes followed by secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... the tissues were incubated with primary mouse anti–α-actinin (sarcomeric) antibody (1:750, Sigma-Aldrich, cat. no. A7811), and vimentin (Abcam ...
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bioRxiv - Cell Biology 2023Quote: Pancreas islets were isolated from mouse (8 weeks male C57BL/6J) pancreas by collagenase (1 mg/ml, Sigma-Aldrich) injection in the bile duct and handpicked under a binocular microscope (Leica) ...
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bioRxiv - Pathology 2023Quote: Concentration of APLN in the medium and in mouse plasma was determined using APLN EIA Kit (RAB0018, Sigma-aldrich) using the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Each mouse was treated with 500μg 6-OHDA (dissolved in 200 μl sterile PBS containing 0.01% ascorbic acid; Sigma-Aldrich), administered by subcutaneous injection daily for 2 days ...
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bioRxiv - Immunology 2023Quote: ... mouse anti-β-tubulin (#T8328) and rabbit anti-phospho-TDP-43 (#SAB4200223) from Sigma Aldrich (St. Louis, MO, USA). Secondary antibodies labelled with horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2022Quote: ... the HBGA phenotype was detected with horseradish peroxidase conjugated with goat anti-mouse IgG (1:1500; Sigma-Aldrich, USA) for type A ...
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bioRxiv - Genomics 2023Quote: E14 mouse embryonic stem cells (E14Tg2a.4) (RRID:MMRRC_015890-UCD) from Mutant Mouse Regional Resource Centers were maintained on 0.1% gelatin (Sigma-Aldrich G1393) coated 6-well plates under the serum/LIF condition containing 15% ES-grade FBS (Gibco 16141061) ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 4 μl (× n samples) of monoclonal anti-Flag M2 antibodies produced in mouse (Sigma-Aldrich). The suspension was moved to 4 °C and rotated for >3 h to conjugate antibodies to magnetic beads ...
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bioRxiv - Developmental Biology 2023Quote: ... incubated overnight at 4°C in blocking buffer with primary antibody (1:500 mouse anti-Elastin, Millipore Sigma MAB2503), washed ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunostaining for NOTCH3 was performed using mouse monoclonal NOTCH3/N3ECD primary antibody (MABC594, Millipore Sigma, 1:25-1:100) with incubation for 32min following CC1 antigen retrieval for 32min ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was immunoprecipitated with anti-PPARγ (A3409A, Perseus Proteomics, Tokyo, Japan) or anti-mouse IgG (Millipore, 12-371) antibody ...
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bioRxiv - Microbiology 2023Quote: ... NIH/3T3 embryonic mouse fibroblast cells (ATCC CRL-1658) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM high glucose; Sigma D6329) supplemented with 10% fetal bovine serum (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... USA) with the mouse Tumor Dissociation kit (Miltenui Biotec) and lymphocytes were enriched on a Ficoll gradient (Sigma-Aldrich). T cells were stained using anti-CD3-Alexa Fluor 488 ...
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bioRxiv - Microbiology 2023Quote: The following primary antibodies were used for immunoblotting: mouse monoclonal anti-α- tubulin (#T5168, Sigma-Aldrich, dilution 1:1,000), rabbit polyclonal anti-MX1(MxA ...