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Citations for Millipore Sigma :
9051 - 9100 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The blocked membrane was incubated with the primary antibodies: mouse anti-HA (2,000×, Sigma-Aldrich HA-7, Massachusetts, USA), mouse anti-α-actin (20,000× ...
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bioRxiv - Pathology 2022Quote: ... The PLA was conducted according to the manufacturer’s instructions using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Cat. No. DUO92101, SIGMA). After blocking for 30 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and Melan-a (pigmented a from Black C57BL/6J mice),49,50 mouse melanocytes were grown in RPMI-1640 medium (Sigma) supplemented with 10% not-heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2, Sigma-Aldrich) in complete RPMI-1640 culture medium supplemented with 10 μM BrdU ...
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bioRxiv - Immunology 2022Quote: ... and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2, Sigma-Aldrich) in complete RPMI-1640 culture medium ...
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bioRxiv - Cell Biology 2022Quote: ... Dilutions of antibodies used for immunoblotting were as follows: mouse anti-Flag at 1:7,500 (clone M2; Sigma-Aldrich), mouse anti-Myc at 1:1000 (9E10 ...
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bioRxiv - Neuroscience 2022Quote: Mouse monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody (diluted 1:200; cat. no. G3893; Sigma-Aldrich, MO, USA) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2022Quote: ... The mouse E14TG2a male ESC line originated from the European Collection of Authenticated Cell Cultures (Sigma 08021401, Lot #15H010). The E14TG2a ESCs were cultured in gelatin-coated (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with primary antibody in blocking solution (1:500 mouse anti-gamma tubulin (Sigma Aldrich, GTU-88), 1:3000 mouse anti-ARL13B (Biolegend ...
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bioRxiv - Cell Biology 2019Quote: ... 3% goat serum) and then incubated with mouse anti-tropomyosin-5 antibody (1:200; clone 2G10.2, Millipore, Burlington, MA) which recognizes Tpm3.1 and Tpm3.2 ...
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bioRxiv - Genomics 2020Quote: ... Immunoprecipitation of the Spo11-oligo complexes was performed using 2.5 μg of monoclonal mouse anti-Flag M2 antibody (Sigma). Precipitated complexes were end-labeled with 5 μCi of [α-32P]dCTP and 5 units of terminal deoxynucleotidyl transferase (Enzymatics) ...
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bioRxiv - Neuroscience 2019Quote: ... Cleared supernatants (500 μg) were mixed with 2 μg IgG control (Sigma Aldrich, 12-371 (normal mouse IgG control) or 12-370 (normal rabbit IgG control) ...
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bioRxiv - Bioengineering 2019Quote: ... The antibodies used were mouse anti-human HSA (1:500; clone HSA-11, A6684; Sigma Aldrich; St. Louis, MO) and 10 nm colloidal gold-conjugated goat anti-mouse IgG (1:40 ...
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bioRxiv - Microbiology 2019Quote: ... Expression of MxA proteins was detected by Western blot analysis using mouse ANTI-FLAG® monoclonal antibody F4042 (Sigma) and rabbit anti-beta-actin polyclonal antibody ab8227 (Abcam ...
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bioRxiv - Zoology 2019Quote: ... animals were preincubated for 30 min at room temperature in PTx+NGS (5% Normal Goat Serum in PBS + 0.1% Triton X-100) and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin ...
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bioRxiv - Pathology 2020Quote: ... Adiponectin was measured in the samples using MILLIPLEX Mouse Cytokine/Chemokine 1-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed 3X with tris-buffered saline with 0.05% Tween-20 (TBST) and incubated with anti-mouse secondary antibody (Sigma) at 1:50,000 for 1 hour at RT ...
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bioRxiv - Developmental Biology 2019Quote: ... expression of Flag-tagged UNC-3 in the total cell lysate was detected using mouse anti-Flag (Sigma, #F3165), expression of Flag-tagged UNC-3 in the IP was detected using rabbit anti-Flag (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... mouse lung tissues were diced into small pieces and further digested with 1x Liberase (0.25U/ml) (Sigma-Aldrich, 5401127001) on a Thermomixer at 37°C for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated overnight in block at 4°C in monoclonal mouse cre recombinase antibody (Millipore MAB 3120) diluted 1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Cytokines and chemokines were assessed using the MILLIPLEX-MAP-Mouse-Cytokiine/Chemokine-Magnetic-Bead-Panel (MCYTOMAG-70K, EMD Millipore). This multiplex panel allows the detection of 25 different cytokines/chemokines and includes individual quality controls for each cytokine/chemokine ...
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bioRxiv - Neuroscience 2019Quote: DNA from offspring was extracted from mouse ear punches using Extract-N-Amp® kit (Sigma, St. Louis, MO) and genotyped using a multiplex PCR reaction ...
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bioRxiv - Cell Biology 2020Quote: Human 293T and mouse P19 cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by washing and staining with horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG secondary antibodies (1:5,000 dilution; Merck Millipore), respectively ...
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bioRxiv - Immunology 2020Quote: ... ROP5-His6-3xFlag complementation strains were assessed by IFA with a mouse anti-Flag primary antibody (Sigma, clone M2) at 1:500 dilution and Alexa Flour 594 goat anti-mouse IgG secondary antibody (Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... The proximity ligation assay was performed using a Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich DUO92101). The cells were blocked with a solution provided by the kit and then incubated overnight at 4°C in a 1:500 dilution of primary antibodies for p53 and Parkin ...
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bioRxiv - Epidemiology 2020Quote: ... An in-house polyclonal anti-flavivirus antibody (anti-DENV 1-4) and an anti-mouse IgG-FITC antibody (Sigma) were used ...
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bioRxiv - Developmental Biology 2019Quote: The primary antibodies and concentrations used were: mouse anti-alpha tubulin (1:500; Sigma-Aldrich, Inc. Cat.# T9026. RRID:AB_477593). Secondary antibodies are listed in the Key Resources table.
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bioRxiv - Developmental Biology 2020Quote: Mouse testes were collected and homogenized in RIPA-Buffer supplemented with protease inhibitor cocktail (Sigma-Aldrich, St. Louis, USA). Following sonication and centrifugation ...
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bioRxiv - Synthetic Biology 2019Quote: ... After rinsing 30 mL of 3% BSA TBST buffer with 0.0625 µL of anti-mouse antibody (Sigma-Aldrich, A9044) was added ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used for experiments in this work: polyglutamylated tubulin (mouse monoclonal antibody, clone B3 T9822, Sigma), pan-polyglycylated antibody (mouse monoclonal antibody ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and eluates were separated by SDS-PAGE and analyzed by Western blotting using mouse α-FLAG M2 (Sigma #F1804).
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bioRxiv - Neuroscience 2020Quote: Whole-cell lysates were prepared from whole mouse brains using CST buffer (Cell Signal Technology) containing protease inhibitor (Sigma), and were rotated at 4°C for 30 minutes before DNA was pelleted ...
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bioRxiv - Immunology 2019Quote: ... Horseradish peroxidase (HRP)-linked goat anti-rabbit and goat anti-mouse IgG secondary antibodies were purchased from Sigma-Aldrich.
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bioRxiv - Immunology 2019Quote: ... resuspended in RPMI-10% FBS containing 40ng/mL CTLA4-Ig (CD152/Fc chimera, non-cytolytic, from mouse, Sigma C4358), plated in 24-well culture plates at a density of 2 x 106 cells/well and cultured for an additional 24 hours (BMDCCTLA4-Ig) ...
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bioRxiv - Biochemistry 2020Quote: ... to detect the nanobodies and secondary antibody anti-mouse coupled to horse radish peroxidase (HRP) (0.5 μg/ml – Millipore). 50 μl of 1-Step UltraTMB-ELISA (ThermoScientific ...
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bioRxiv - Microbiology 2019Quote: ... overnight at 4°C at a 1:1000 at dilution and α-mouse IgG (whole molecule)-peroxidase (Sigma #9044) for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... After incubation at room temperate for 2 hr with a 1:5,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times for 5 min and incubated with anti-mouse-HRP antibody (0.2 μg/ml – Millipore) for 1 h in TBST ...
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bioRxiv - Immunology 2019Quote: ... mouse peritoneal neutrophils were harvested 4 h after intraperitoneal injection of 2 ml of 4% thioglycollate broth (Sigma-Aldrich). The collected peritoneal neutrophils were cultured in RPMI 1640 (Welgene ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBST (1:2,000 for mouse anti-GFAP, Sigma; 1:1,000 for chicken anti-GFAP ...
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bioRxiv - Microbiology 2019Quote: ... injection with 200 μL per mouse of a 1:1 (v/v) mixture of Freund’s complete adjuvant (Sigma-Aldrich) and a solution containing 250 μg of purified rTMPT2A ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated overnight at 4°C in mouse anti-NeuN primary antibody (#MAB377, RRID:AB_2298772, Millipore Sigma, Billerica, MA) at a 1:1000 concentration in diluted blocking solution (10% fetal plus ...
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bioRxiv - Evolutionary Biology 2021Quote: Pulldown experiments assayed by western blotting were visualized using a commercially available mixture of Mouse ⍺-GFP clones 7.1 and 13.1 (Sigma-Aldrich) at a dilution of 1:103 for the primary antibody incubation followed by secondary incubation with goat ⍺-mouse conjugated to horseradish peroxidase (1:104 dilution ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extraction from skelatal muscle tissue or C2C12 mouse myoblasts was done using TRI Reagent (Millipore-Sigma #T9424). Muscle tissue samples were flash-frozen in liquid nitrogen until further processing ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extraction from skelatal muscle tissue or C2C12 mouse myoblasts was done using TRI Reagent (Millipore-Sigma #T9424). Muscle tissue samples were flash-frozen in liquid nitrogen until further processing ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5 μl blocking buffer (10 mg/ml mouse IgG with 5 mM Azide) and 0.5 μl 0.5% Phenol red (Sigma P0290) were added for blocking the unconjugated Anti-Mouse-IgG-Atto647N and vortexed thoroughly ...
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bioRxiv - Molecular Biology 2020Quote: ... blocked with 5% non-fat dry milk in TBS-0.1% Tween-20 for 1 h and incubated overnight with mouse anti-ataxin-3 (1:1000; Millipore), mouse anti-HA (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... the slides were incubated in primary antibody (Mouse anti-TH antibody, 1:1000, product #MAB5280, Millipore, Billerica, MA, USA) for 1 hour at room temperature and 36 hours at 4° C ...
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bioRxiv - Neuroscience 2020Quote: ... or AAVretro-EF1a-Cre primary antiserum (1:100 mouse-anti-Cre Recombinase antibody, clone 2D8, Millipore Sigma Cat# MAB3120) mixed with donkey serum ...