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Citations for Millipore Sigma :
9001 - 9050 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated in TBST-5 % w/v milk (TBST supplemented with 5 % (w/v) skimmed milk powder) with anti-rabbit-HRP secondary antibody (A0545, Sigma Aldrich) (1:5,000 ...
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bioRxiv - Developmental Biology 2019Quote: ... dishes were washed twice for 5 minutes with 0.002% Triton X-100 in PBS and incubated with anti-rabbit-PLUS (Sigma-Aldrich, DUO92002) and anti-mouse-MINUS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... the ligation experiment was performed according to the manufacturer’s instructions using the Duolink® In Situ Orange Starter Kit Goat/Rabbit (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred to a PVDF membrane and incubated with one of the following primary antibodies: α-FLAG polyclonal rabbit (Sigma) or α-RpoA monoclonal mouse (Biolegend) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... directed against the N-terminal half of mouse AHR and the polyclonal goat anti-rabbit secondary antibody cross-linked to alkaline phosphatase (Sigma-Aldrich). The blot was developed using the AP Conjugate Substrate Kit (Bio-Rad).
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bioRxiv - Pathology 2020Quote: ... Membranes were then incubated with the corresponding secondary antibodies (rabbit anti-mouse IgG/HRP, Dako, #P0260 and goat anti-rat IgG/HRP, Sigma, #A9037) at a 1:5000 dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-cleared nuclear lysate was then added to 50 μL beads conjugated with 2-4 μg primary IgG antibody: rabbit anti-APOBEC2 (Sigma, HPA017957) or rabbit IgG isotype control ...
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bioRxiv - Microbiology 2021Quote: In situ proximity ligation assay (PLA) was performed using the Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: For ChIP the following antibodies were used in this study: Rabbit polyclonal anti H3K9/K14-ac (Merck Millipore; Cat#06-599); Rabbit monoclonal anti H3K4-me3 (clone MC315 ...
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bioRxiv - Immunology 2019Quote: ... Beads were harvested and boiled in 40 μL of Laemmli sample buffer prior to western blot analysis with a polyclonal rabbit anti-PTPN2 antibody (SAB4200249, Sigma-Aldrich).
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... Blotted proteins were detected with a monoclonal antibody that recognizes SARS-CoV-2 S1 (SinoBiological) and a secondary anti-rabbit horse radish peroxidase (HRP)-conjugated antibody (Sigma Aldrich). Blots were developed with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: Proximity ligation assay (PLA) was performed using the Duolink In Situ Red Started Kit Mouse/Rabbit (Millipore Sigma, Cat# DUO92101-1KT) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Magnetic protein G beads were washed with blocking solution (0.5% BSA in PBS) and then incubated with rabbit antiserum against acetylated histone H4 (Millipore 06-866) or normal rabbit anti-serum in blocking solution for several hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... Surviving clones were screened via immunoblotting targeting TANGO1L (2 ng per mL rabbit polyclonal anti-MIA3, Sigma-Aldrich Prestige, HPA056816-100UL) and a rabbit polyclonal targeting anti-TANGO1-CC1 (1211 – 1440aa/exon9-15 shared between both TANGO1S/L (a gift from Kota Saito ...
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bioRxiv - Microbiology 2022Quote: ... α-PilC 20 and α-RomY antibodies were used together with goat anti-rabbit immunoglobulin G conjugated with horseradish peroxidase (Sigma) as secondary antibody ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed and probed with either goat anti-mouse horseradish peroxidase or goat anti-rabbit horseradish peroxidase (Sigma-Aldrich) at a dilution of 1:10,000 for 1 h (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... Chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated with the indicated antibodies or rabbit preimmune sera complexed with Protein A agarose beads (Sigma-Aldrich). Immunoprecipitated complexes were sequentially washed with Wash Buffer A ...
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bioRxiv - Microbiology 2023Quote: ... The interactions between the two labeled proteins were detected using the Duolink in Situ Red Starter Kit Mouse/Rabbit (#DUO92101-1kit, Sigma-Aldrich) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... A stock ATPase reaction mix (20X) contained 20 μL of a mixture of pyruvate kinase and lactic dehydrogenase from rabbit muscle (P0294, Sigma Aldrich), 10 μL of 200 mM NADH (CAS# 606688) ...
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bioRxiv - Molecular Biology 2024Quote: ... Mouse monoclonal anti-Flag (M2), mouse monoclonal anti-Actin (MAB1501, clone C4) and rabbit polyclonal anti-GST (G7781) were from Sigma-Aldrich. Rabbit polyclonal anti-FOXK1 (MNF ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in blocking solution (5% normal donkey serum and 0.3% Triton X-100 in PBS) for 30 minutes and then incubated in rabbit anti-TH polyclonal antibody (Millipore, Cat: AB152) diluted 1:1000 in 0.3% Triton X-100 in PBS ...
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bioRxiv - Microbiology 2024Quote: ... HRP-conjugated anti-mouse secondary antibody (57404) was purchased from SRL and anti-rabbit secondary antibodies were purchased from Sigma (A8275). Alexa Fluor 488 goat anti-mouse (A11001) ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...
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bioRxiv - Cell Biology 2024Quote: Samples were incubated with secondary antibodies conjugated with PLA probes MINUS and PLUS: the PLA Probe anti-Mouse PLUS and anti-Rabbit Minus (Sigma-Aldrich). The incubation with all antibodies was carried out in a humidified chamber for 1 h at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times for five min each with TBST and then incubated with secondary antibodies (goat anti-rabbit IgG HRP, A0545, Sigma Aldrich; goat anti-mouse IgG HRP ...
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bioRxiv - Biochemistry 2024Quote: ... After incubation with horse-radish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit-HRP and goat anti-mouse-HRP, Sigma-Aldrich) at room temperature for 1 h ...
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bioRxiv - Biochemistry 2023Quote: GS activities were measured using the pyruvate kinase/ lactate dehydrogenase (PK/LDH) coupled enzymes from rabbit muscle ordered from Sigma-Aldrich (containing 600-1,000 units/ml pyruvate kinase 900-1,400 units/ml lactate dehydrogenase) ...
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bioRxiv - Bioengineering 2023Quote: ... Antibody binding was detected using HRP-conjugated anti-rabbit IgG and visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, Burlington, MA) and an ImageQuant LAS4000 digital imaging system (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA (rabbit monoclonal from Cell Signaling Technology Inc., cat. no. 3724S; and mouse monoclonal from Sigma-Aldrich, cat. no. H3663), anti-myc (mouse monoclonal clone 9E 10 from the University of Iowa Hybridoma Bank) ...
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bioRxiv - Genomics 2023Quote: ... Revelation of the primary antibodies was performed by incubation with goat anti-rabbit Horseradish peroxidase-conjugated secondary antibody (Ref#A0545, Sigma-Aldrick) at 1/10000 ratio in blocking solution for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... pTyr (clone 4G10, 05-321), and HA (clone HA-7, H3663) and rabbit polyclonal anti-SEPTIN12 (HPA041128) were from Sigma-Aldrich. Rabbit and mouse monoclonal anti-DYKDDDDK (clone D6W5B ...
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bioRxiv - Cell Biology 2023Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated in primary antibody solution containing Anti-Neurofilament 200 primary antibody produced in rabbit IgG (N4142, Sigma Aldrich) and biotin-conjugated Anti-NeuN primary antibody produced in mouse IgG (MAB377B ...
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bioRxiv - Microbiology 2023Quote: PLA: PLA was carried out using the Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich, Cat #DUO92101-1KT). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
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bioRxiv - Microbiology 2023Quote: ... anti-p-PKC-δ-T505 (AP0776) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Biochemistry 2023Quote: Duolink proximity ligation assays were performed using a Duolink™ In Situ PLA Kit Goat/Rabbit (Olink Biosciences) purchased from Sigma. Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... rat monoclonal antibody against the N-terminus of DAT (MAB369) and rabbit polyclonal antibody against tyrosine hydroxlase (TH) (AB152) were from EMD Millipore, Rabbit polyclonal antibodies against vacuolar protein sorting-associated protein 35 (VPS35 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Genetics 2023Quote: ... Duolink probes (mouse plus and rabbit minus) were prepared and applied as described by manufacturer’s instructions for the Duolink® PLA kit Cy5 (Millipore-Sigma DUO92013).
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bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin ...
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bioRxiv - Developmental Biology 2024Quote: ... Tween-20 in PBS for 15 min at RT and then incubated with rabbit anti-human RYBP polyclonal antibody (Sigma, HPA053357) and mouse anti-human hCG-β antibody (abcam ...
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bioRxiv - Immunology 2024Quote: ... The wells were rinsed three times with PBS containing 0.05 % Tween 20 and horseradish-peroxidase-labeled rabbit anti-human or anti mouse IgG (100 μL/well; Sigma-Aldrich, Germany) was added to each well and incubated for 1 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002; In-Situ Detection Reagents FarRed, Sigma DUO92013) according to the manufacturer’s in situ fluorescence protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with the secondary antibodies Duolink® In Situ PLA® Probe Anti-Rabbit PLUS (Sigma-Aldrich, DUO92002-100RXN) and Duolink® In Situ PLA® Probe Anti-Mouse MINUS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibody Donkey anti-mouse 488 (Alexa-fluor A32766, Life Technology), Donkey anti- rabbit 555 (Alexa-fluor A32794, Life Technology) and DAPI (MBD0015, Sigma-Aldrich) in blocking buffer were added to the samples and incubated for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... was performed to detect the interactions between ITGβ1 and CD44 using Duolink® In Situ Red Starter Kit Mouse/Rabbit (DUO92101, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were blocked and incubated with the following primary antibodies for 1 hour at RT in humidity chamber (anti-CldU, Abcam ab6326, 1:100; anti-IdU, BD 347580, 1:100; anti-ssDNA, Millipore MAB3034, 1:100). After washes with PBS ...
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bioRxiv - Cell Biology 2019Quote: ... anti PMF1/NNF1 (in house made mouse affinity purified monoclonal; clone RH25-1-54, 1:1000) and anti-Tubulin (mouse monoclonal, Sigma-Aldrich; 1:10000). Quantification of protein levels from western blots was performed with the following formula ...