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Citations for Millipore Sigma :
8951 - 9000 of 10000+ citations for Rabbit Anti Human IgG+IgM+IgA Alexa Fluor 660 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... membrane was further incubated with anti-HA (Abcam)/anti-SUMO1 (in-house produced) and anti-MYC (Sigma) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-STIM1 (Novus) or mouse anti-FLAG (Sigma) were used for IF staining ...
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bioRxiv - Cell Biology 2023Quote: ... the anti-ATG7 and anti-ATG13 are from Sigma (cat ...
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bioRxiv - Neuroscience 2024Quote: ... Monoclonal anti-S100β antibodies (mouse anti-S100β, Sigma Aldrich #S2532 or rabbit anti-S100β ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies (Anti-3-hydroxybutyryllysine [PTM Biolabs 1201], anti-p84 [GeneTex GTX70220], anti-α-Tubulin [Sigma T5168] ...
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bioRxiv - Plant Biology 2024Quote: ... anti-HA antibodies (Anti-HA High Affinity Roche 3F/10, Sigma-Aldrich, and anti-HA Abcam). At least three independent biological replicates were performed per experiment.
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation at 8000 g for 10 min and pre-cleared by pre-incubated with 100 μl Mouse IgG−Agarose beads (A0919 Sigma) without antibody for 2 h at 4°C on wheel ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with an affinity-purified specific antibody (1:10,000) and goat peroxidase-conjugated antirabbit IgG (1:20,000, Sigma-Aldrich, USA), for 2 h at 25 ºC ...
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bioRxiv - Immunology 2022Quote: ... we used the appropriate corresponding isotype control antibodies (mouse IgG1 K and mouse IgG2 B, both from Biolegend, goat IgG from SIGMA) and a drug carrier control.
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bioRxiv - Systems Biology 2020Quote: ... TMT labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (Sigma) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... up to 100 μg of carrier-free IgG antibody was subjected to buffer exchange by washing with R-buffer using a 50 kDa Amicon filter (Millipore) that was pre-soaked with R-buffer ...
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were immunoprecipitated with 2 μg of IgG (negative control) or Smad3 antibody in coimmunoprecipitation reagents (17-195, Millipore). Semi-quantitative and quantitative PCR were performed to amplify α-SMA or Col1A promoter regions containing SBE.
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bioRxiv - Plant Biology 2019Quote: ... four times and then placed for 1 h in a solution of goat antirabbit IgG (dilution 1:17500) conjugated to alkaline phosphatase (Sigma). The membrane was rinsed in TBS-T four times ...
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bioRxiv - Microbiology 2021Quote: ... An α-mouse IgG conjugated to horseradish peroxidase (HRP) was used as the secondary antibody at a dilution of 1:4,000 (Sigma-Aldrich). To assess cell wall fractionation a mouse α-protein A (Spa ...
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bioRxiv - Biochemistry 2022Quote: Two commercially available mAbs (immunoglobulin or IgG, type 1) were used for the fragmentation experiments: Sigma mAb standard (SiLuLite, IgG1 lambda, CHO, Sigma) and NIST mAb standard (HzIgG1 kappa ...
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bioRxiv - Developmental Biology 2023Quote: ... HSF5-N2 and control IgG antibodies were crosslinked to 50 µl of protein A-Dynabeads (Thermo-Fisher) by DMP (Sigma). The antibody-crosslinked beads were added to the testis extracts prepared above ...
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bioRxiv - Cancer Biology 2023Quote: ... TMT-labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (P3300, Sigma) overnight at 4℃ ...
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bioRxiv - Plant Biology 2022Quote: ... Seedlings were then washed 2 times with PBS and cell walls were stained with 0.1% Calco fluor white (Fluorescent brightener 28 F3543-1G Sigma-Aldrich) in PBS for 30 min in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... CuAAC reaction was performed following the protocol from ClickChemistryTools (Cat. No. 1001) and Azide-fluor 488 (Cat. No. 760765, Sigma) was used to react with IA-Alkyne ...
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bioRxiv - Developmental Biology 2023Quote: ... The resultant samples were load on SDS-PAGE and subjected to pIMAGO phospho-protein detection kit with fluor-680 (Sigma) detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... grids were incubated with secondary antibody Alexa 488 and in the last 5 minutes Nile red (Sigma, 72485) and Hoechst 33342 (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... FAK) recombinant proteins to be labeled with Alexa fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Biophysics 2020Quote: ... Micropatterns were printed for 5 min with specifically designed chrome masks and coated for 1h at 5% CO2 and 37°C with 50μg/mL fibronectin and 20μg/mL Alexa 546–fibrinogen (Sigma) diluted in distilled water ...
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bioRxiv - Neuroscience 2024Quote: ... Pellets were resuspended in blocking buffer containing DPBS+BSA with NEUN-Alexa 488 pre-conjugated antibody (Millipore #MAB377X) at 1:1000 dilution along with SOX9 rabbit antibody (Abcam AB185966 ...
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bioRxiv - Cancer Biology 2021Quote: ... after which slides were washed in phosphate-buffered saline and stained against Human Nuclei (MAB4190, 1:250, Millipore) and Human specific Ki67 (MAB4383 ...
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bioRxiv - Cell Biology 2020Quote: ... 25×106 cells were plated on 6-well culture dishes coated with human fibronectin (Sigma, St Louis, MO) and maintained in endothelial cell medium-2 (EGM-2 - Clonetics ...
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bioRxiv - Cell Biology 2020Quote: Senescence in normal human fibroblasts was induced using five treatments with 30 μM tert-butyl hydroperoxide (tBHP, Sigma) for 5 consecutive days (1 h/day ...
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bioRxiv - Immunology 2021Quote: ... were added to either tissue culture-treated plates coated with 10 μg/ml human plasma fibronectin (Sigma-Aldrich) or Corning® Costar® Ultra-Low Attachment plates (Sigma-Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... mice underwent the full superovulation procedure with an intraperitoneal injection of 10 IU human chronic gonadotropin (hCG, Sigma) 48 hours after PMSG administration ...
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bioRxiv - Bioengineering 2019Quote: ... or standard 6-well tissue culture plastic dishes were incubated with human plasma fibronectin (Millipore Sigma, Catalog #FC010) at a concentration of 10 μg/ml diluted in PBS (3 ml/well of ECM protein solution added ...
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bioRxiv - Cell Biology 2019Quote: ... human HT-1080 fibrosarcoma cells (CCL-121; ATCC) were maintained using Eagle’s Minimum Essential Medium (EMEM; Millipore Sigma) supplemented with 2 mM L-glutamine and containing 10% (vol/vol ...
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bioRxiv - Genomics 2019Quote: ... we measured 41 different cytokines and chemokines using the Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore kit no ...
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bioRxiv - Biochemistry 2021Quote: ... reactions were then incubated in the presence of 50 μg/mL (~0.1 units) human salivary α-amylase (Sigma) or 1.5 M hydroxylamine for a further 60 min at 37°C ...
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bioRxiv - Genetics 2021Quote: ... AG1-0 cells were passaged according to the product manual for PluriSTEM Human ES/iPS Medium (Millipore Sigma), which is feeder-free and utilizes Dispase II (Millipore Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... and cultured in Human IntestiCult™ Organoid Growth Medium (StemCell) supplemented with 10 mmol/L Y-27632 (Sigma), 5 mmol/L CHIR99021 (ReproCell ...
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bioRxiv - Neuroscience 2021Quote: ... the eye cup was treated for ∼15 minutes with human plasmin (∼50 μg/mL, Sigma or Haematologic Technologies) to aid vitreous removal ...
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bioRxiv - Cell Biology 2019Quote: ... either directly or after coating with 10 µg/ml human plasma fibronectin (cat no. FC010; Millipore, Billerica, MA), as noted in the figure legend.
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bioRxiv - Biochemistry 2020Quote: DNA sequences containing human SUN2 were cloned into a modified bicistronic ampicillin resistant pETDuet-1 vector (EMD Millipore), superfolder GFP-KASH fusions (sfGFP ...
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bioRxiv - Molecular Biology 2021Quote: Nuclei from human liver (15 mg) were isolated using with the Nuclei EZ Prep kit (Sigma-Aldrich, #NUC101) and a glass dounce homogenizer (25 strokes with lose pastel and 25 strokes with tight pastel in ice-cold Nuclei EZ Lysis Buffer) ...
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bioRxiv - Neuroscience 2020Quote: Chromatin for ChIP was prepared from immortalized human microglia using the Imprint® Chromatin Immunoprecipitation kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... shRNAs stably integrated in mouse and human cells were selected with 10 and 2 μg/ml puromycin (Sigma), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Human colon cancer cell lines HCT116 TP53+/+ and HCT116 TP53-/- were grown in McCoy’s 5A Medium (Sigma, M9309) supplemented with 10% FBS and Pen/Strep ...
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bioRxiv - Cell Biology 2021Quote: ... α-taxilin (NM_175852.4) and its truncated segments were amplified from human cDNA and cloned into pcDNA3.1(+) (Sigma-Aldrich). Full-length α-taxilin and γ-taxilin were also cloned into p3×FLAG-CMV-14 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa D2 parental control cells were transfected with 300 ng/ml human VRK1 MISSION® esiRNA (EHU119541, Sigma) using HiPerFect Transfection Reagent (301707 ...
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bioRxiv - Microbiology 2021Quote: ... HMEC-1 cells (human microvascular endothelial cells RRID:CVCL_0307) were grown in HMEC media (MCDB 131 media (Sigma, M8537) supplemented with 10% FBS (HyClone ...
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bioRxiv - Microbiology 2021Quote: Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma, Cat#M4655) supplemented with 10% (upon and after viral infection ...
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bioRxiv - Microbiology 2021Quote: ... Then the inoculum was aspirated and DMEM supplemented with 5% NBCS and 7.5 µg/mL human immunoglobulin (Sigma) was added ...
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bioRxiv - Microbiology 2021Quote: Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma, Cat#M4655) supplemented with 10% (upon and after viral infection ...
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bioRxiv - Microbiology 2021Quote: ... and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma), 100U/mL P/S ...