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Citations for Millipore Sigma :
8751 - 8800 of 10000+ citations for Rabbit Anti Human IgG+IgM+IgA Alexa Fluor 660 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... F9 and WEHI-164 tumors was studied on ice-cold acetone-fixed 8-10μm cryostat sections stained with FITC labeled IgG(L19) (FITC_F7250 Sigma) (final concentration 2μg/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... Background precipitation levels were determined by performing mock precipitations with a non-specific IgG antiserum (Sigma C-2288) and were only a fraction of the precipitation levels obtained with the specific antibodies ...
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bioRxiv - Immunology 2020Quote: ... eluted with IgG elution buffer and concentrated using 10-kDa Amicon ultra-15 centrifugal filter units (EMD Millipore).
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bioRxiv - Biochemistry 2023Quote: ... The 139H2 membranes were washed again with TSMT and incubated with α-mouse IgG secondary antibody (A2304, Sigma) diluted 1:8000 in TSMT with 1% BSA for 1 h at RT ...
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bioRxiv - Biophysics 2023Quote: ... Elution fractions containing highest concentrations of IgG were pooled and concentrated using Amicon Ultra-15 centrifugal filters (Millipore). The final concentration of purified mouse IgG was determined with a Bradford assay ...
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bioRxiv - Biochemistry 2023Quote: ... All assays were conducted using FP buffer [SEC buffer plus 0.1 mg mL-1 bovine IgG (Sigma-Aldrich) and 0.5 mM Thesit (Sigma-Aldrich)] Binding affinities were determined using SOLVER ...
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bioRxiv - Synthetic Biology 2024Quote: ... and incubated for 2 h in 1:20,000 horseradish peroxidase (HRP)-conjugated goat α-mouse IgG (Sigma-Aldrich)/1% BSA/TBS ...
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bioRxiv - Cell Biology 2023Quote: ... added at the time of treatments and labeled by the click reaction with Azide Fluor 545 (Sigma Aldrich) as reported (14,19) ...
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bioRxiv - Cancer Biology 2022Quote: ... secondary proximity probes binding rabbit and mouse immunoglobulin (PLA probe rabbit PLUS and PLA probe mouse MINUS, Olink Bioscience, Sigma-Aldrich, St. Louis, MO, USA) were diluted 1:15 and 1:5 in blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-phosphotyrosine (anti-pY) clone 4G10 and anti-facilitative glucose transporter 3 (anti-GLUT3) AB1344 from Millipore (Temecula, CA, USA); anti-phospho PKA substrates (anti-pPKAs ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-MYC (Cell Singaling) or anti-FLAG (Sigma) antibodies ...
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bioRxiv - Genetics 2019Quote: ... anti-α-tubulin (1:20,000, Sigma, anti-mouse), IRDye 800CW (1:20,000 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-goat and anti-mouse antibodies (Sigma-Aldrich, cat ...
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bioRxiv - Cell Biology 2022Quote: ... anti-actin and anti-ß-tubulin from Sigma, anti-S-tag from Novagen ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... anti-MCT1 (chicken anti-mouse, 1:200, Sigma) and anti-pimonidazole conjugated with red fluorophore (mouse anti-pimonidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-SMC3 (homemade) or anti-PAXIP1 (Sigma #ABE1877). Protein-antibody complexes were precipitated with 10µL of protein G Dynabeads (Fisher #10003D) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Sequestosome-1 (anti-SQSTM1/p62, Sigma, P0067), anti-phosphoinositide 3-kinase regulatory subunit 4 (anti-PIK3R4/VPS15 ...
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bioRxiv - Microbiology 2020Quote: ... For all ChIP experiments a mock IP and an experimental IP were set up at the same time using 1,350 µl pre-cleared chromatin and 10 µg of normal mouse IgG (Millipore) and monoclonal mouse anti-c-Myc respectively ...
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatants were pre-cleared twice by incubation with 28 μL 50% mouse IgG agarose slurry (Sigma #A0919) for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... were coated with 3 μg of specific antibody or control IgG by resuspending them in 60 μl PBS containing 0.02% Tween-20 (P1379, Sigma), and rotated for 45 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 1000µg of the soluble fraction were taken for IP using 2µg of YAP antibody (NB110-58358, Novus) or IgG control (#I5006, Sigma), and 25µl of magnetic beads ...
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bioRxiv - Plant Biology 2021Quote: ... For Co-IPs with MED10B-MEC-ProtA we used IgG Sepharose® 6 Fast Flow (Sigma, GE17-0969-01) beads and increased washing steps (1xIP buffer ...
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bioRxiv - Microbiology 2019Quote: ... overnight at 4°C at a 1:1000 at dilution and α-mouse IgG (whole molecule)-peroxidase (Sigma #9044) for 1 h ...
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bioRxiv - Biochemistry 2019Quote: ... ∼500-ml cell suspension containing the secreted IgG was clarified by centrifugation and subsequent filtration through a 0.45 µm HAWP membrane (Merck Millipore). The filtrate was mixed with an equal volume of binding buffer (20 mM Na2HPO4/NaH2PO4 ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5 μl blocking buffer (10 mg/ml mouse IgG with 5 mM Azide) and 0.5 μl 0.5% Phenol red (Sigma P0290) were added for blocking the unconjugated Anti-Mouse-IgG-Atto647N and vortexed thoroughly ...
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bioRxiv - Neuroscience 2022Quote: ... Two additional 0.5 mL solubilisates of cerebellum and midbrain samples were incubated with 7.5 µg IgG (Millipore, 12-370), which were used as internal specificity control ...
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bioRxiv - Immunology 2022Quote: ... Fractions containing IgG were identified by SDS-PAGE under reducing/non-reducing conditions and concentrated using Centrifugal Filters (Millipore). Protein concentration was quantified with the BCA Assay Kit (Beyotime ...
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bioRxiv - Molecular Biology 2023Quote: ... The beads were separated on a magnet and resuspended in 25 µl of cold Antibody buffer (Wash buffer with 0.1% BSA) containing respective primary antibody or IgG (TFE3, Sigma HPA023881 ...
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bioRxiv - Cell Biology 2023Quote: ... Tetraspanin and mouse IgG (mIgG) beads were washed in PBS with 0.1% (m/v) bovine serum albumin (BSA) (Sigma) prior to use ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous SA1 and SA2 were immunoprecipitated with 10 µg SA1 and SA2 antibodies or non-specific IgG control (Sigma) conjugated to protein G dynabeads (Dynal ...
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bioRxiv - Molecular Biology 2022Quote: ... Approximately 150 synchronized L4 worms were transferred to plates supplemented with 0.5 μg/ml of 5-fluor-2-deoxyuridine - FUdR (Sigma), an inhibitor of mitosis ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and incubated with Alexa-488 conjugated secondary antibody (ThermoScientific, USA) and DAPI (Sigma, USA). The coverslips were mounted using glycerol and imaged on an Axio Imager Z2 microscope (Zeiss ...
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bioRxiv - Biophysics 2019Quote: ... and then coated with 50 μg/mL fibronectin and 20 μg/mL Alexa 546–fibrinogen-red (Sigma). Cells were seeded on freshly prepared protein-coated micropatterns and allowed to spread for at least 2–3 hrs before experiment started 50 ...
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bioRxiv - Biophysics 2020Quote: ... then immersed in a solution of Human Plasma Fibronectin (10 ug/mL in PBS; #FC010, EMD Millipore), and washed thoroughly in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were probed with antibody specific for human P2X4 (Cat. No. HPA039494, Sigma-Aldrich, St. Louis, MO), mouse P2X4 (Cat ...
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bioRxiv - Microbiology 2021Quote: ... in PBS for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) for 1 hour at room temperature ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... hiPSCs were plated at a density of 4-5×104 cells/cm2 onto a human fibronectin (Millipore)-coated 12 well plate in GK15 medium (GMEM [Life Technologies] with 15% KSR ...
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bioRxiv - Neuroscience 2021Quote: ... A 100x high purity N2 solution was made with 10 mg/ml human holo-transferrin (Sigma T4132), 0.5mg/ml human recombinant insulin solution (Sigma I9278) ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... The DNA-binding domain of human Timeless (DBD; residues 816-954) was cloned into pRSF-Duet1 (Novagen) with an N-terminal His14-SUMO tag and expressed in E ...
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bioRxiv - Immunology 2020Quote: ... T cells were cultured in Click’s medium supplemented with 10% heat-inactivated human serum (Sigma-Aldrich, H4522), 1% L-glutamine and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... and primary human monocytes were treated with 100 ng/ml LPS (O26:B6) (Sigma Aldrich; Cat.# L2654) for 3-4 hrs ...
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bioRxiv - Cell Biology 2019Quote: ... 4H1 mouse monoclonal antibody targeting amino acids 35 to 44 of human ORF1p40,67,68 (Millipore, cat. number MABC1152) was used at 1:15000 dilution ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... mice were further injected with 5 IU (0.1 mL/animal) human chorionic gonadotropin (hCG) (Sigma-Aldrich, USA). Twenty hours after PMSG injection ...
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bioRxiv - Microbiology 2019Quote: ... Parasites were grown in human O+ erythrocytes at 4% hematocrit in complete RPMI1640-HEPES media (Sigma-Aldrich) supplemented with 0.3% L-glutamine ...
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bioRxiv - Microbiology 2019Quote: ... Routine plaque assays were performed in duplicate in the presence of 1% pooled neutralising human serum (Sigma). Single-step infections were performed at moi 5 or 10 pfu/cell and multi-round infections at a moi of 0.005 pfu/cell ...
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bioRxiv - Microbiology 2019Quote: Human foreskin fibroblasts (HFFs) were cultured in minimum essential medium Eagle (MEM; Sigma-Aldrich, St. Louis, Missouri) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: Human HepG2 cell line was grown in Dulbecco’s Modified Eagle’s medium – high glucose (Sigma-Aldrich®, D6429) supplemented with 10% fetal bovine serum (PAN Biotech ...
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bioRxiv - Bioengineering 2019Quote: ... and fibrinogen (FBG; from human plasma, 20 mM sodium citrate-HCl pH 7.4, Lot #3169957, Millipore Sigma). FAM-proteins were prepared with 10 mg of protein reconstituted in 900 μL of 0.1 M PBS and 8-fold molar excess of FAM NHS ester solubilized in 100 μL DMSO ...
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bioRxiv - Immunology 2019Quote: ... human liver tissue was minced with scissors and digested in 5mg/ml pronase (Sigma-Aldrich, P5147-5G), 2.93mg/ml collagenase B (Roche ...