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Citations for Millipore Sigma :
8951 - 9000 of 10000+ citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 CRASP 1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The blot was stripped and then reprobed for FLAG-tagged receptor using anti-FLAG antibody (1:2000, Sigma, Cat. no. A8592). Data were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... the membranes were washed with TBST buffer for three times and subsequently incubated in 1:10,000 dilution of secondary antibody (anti-mouse IgG whole molecule conjugated with alkaline phosphatase) (A3562, Sigma-Aldrich, USA). The membranes were then washed with TBST buffer for three times and detected using AP detection kit (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: ... and mouse monoclonal anti-DNA/Histona H1 antibody (1:100; catalog number 05-457/clone AE-4; Merck Millipore, MA, EUA). Alternatively ...
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bioRxiv - Bioengineering 2019Quote: ... was incubated in 5% milk powder in TBS-t buffer with anti-tubulin primary antibody (1:500, Sigma #T9026, Buchs, Switzerland)) ...
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bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
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bioRxiv - Developmental Biology 2020Quote: ... terminus or c.terminus anti-SPAG17 antibodies (previously described by Zhang and colleague, Zhang, Jones et al. 2005) and 1:1000 Mouse anti-Tubulin (Sigma #T6199) antibodies) ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with the secondary antibody (anti-Mouse-HRP IgG, 1:10,000, Sigma-Aldrich, Saint Louis, MO, USA) diluted in TBS-T for 1 hour ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... then blocked with 4% NHS/TBST-0.2% and incubated overnight at 4°C in primary antibody solution (1:2500 anti-tyrosine hydroxylase, clone LNC1, Millipore, Billerica, MA) in 2% NHS/TBST-0.2% ...
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bioRxiv - Molecular Biology 2020Quote: Antibodies and dilutions for IF and Duolink were the following: mouse monoclonal anti-RPAP3 at 1:250 dilution (Sigma-Aldrich, SAB407956); polyclonal rabbit anti-TRBP at 1:100 dilution (Abcam ...
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bioRxiv - Bioengineering 2021Quote: ... the cultures were fixed with 4% paraformaldehyde and immunolabeled with anti-neurofilament 200 (NF200) antibodies (1:400, Sigma-Aldrich, catalog # N0142) followed by an Alexa 488 or Alexa 568 conjugated secondary antibody (1:800 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation at 4°C in a 1:500 dilution of the anti-Calbindin-D-28K antibody (Sigma, CB-955) in blocking solution for 48 hours ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma, F3165). Each reaction was incubated with Protein-G agarose beads (50 μL ...
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bioRxiv - Genetics 2021Quote: ... was used for staining DNA and mouse anti-a tubulin monoclonal antibody DM1A (1:50) conjugated with FITC (Sigma, St. Louis) was used for staining microtubules ...
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bioRxiv - Physiology 2021Quote: ... Sections were then incubated for 45 minutes at 37°C with the primary antibody at 1:100 for anti-laminin (L9393, Sigma-Aldrich). Three washes were then performed with PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... plus 0.1 % Triton X-100 and incubated overnight at 4 °C or for 2 h at room temperature with the following primary antibodies: anti-REST (1:500; 07-579 Millipore, MA), anti-Npas4 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.4) for 1 hour at room temperature and then incubated at 4°C overnight with primary antibodies (anti-multicillin, Sigma Aldrich, HPA058073 1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were blocked in 5 % skimmed milk in PBS prior to incubation with primary antibody (anti-APP A8717 1:5000 Sigma Aldrich) at 4°C overnight prior to incubation with anti-rabbit HRP ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated with primary antibodies diluted in 5% milk overnight at 4°C (anti-ATG13 (SAB4200135, 1:100, Sigma, MI, USA); anti-GAPDH (10494-1-AP ...
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bioRxiv - Biophysics 2020Quote: ... The cells were then washed with 1× PBS 3 times and immunofluorescence staining was performed using mouse anti-alpha tubulin primary antibody (T5168, Sigma Aldrich) at 1:1000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, A8592, 1:5,000 dilution) and TMB substrate ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were blocked in %5 BSA for 2 h at room temperature and incubated with anti-desmin antibody (1:20; Sigma, D8281) for two overnights ...
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bioRxiv - Developmental Biology 2021Quote: ... blocked for one hour at room temperature (%5 nonfat dried milk in %0,2 TBS-T) and probed with anti-desmin antibody (1:1000; Sigma-Aldrich, D8281) overnight at 4°C or anti-lamin B1 antibody (Abcam ...
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bioRxiv - Genomics 2020Quote: ... permeabilized with 0.2% Triton X-100 in PBS and then stained with primary antibody specific for Cy3-conjugated anti-αSMA (1:400, Sigma, #C6198), rat anti-PDGFRβ (1:200 ...
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bioRxiv - Genomics 2020Quote: ... Incubation with monoclonal anti-ATPIF1 (ATP synthase subunit IF1) antibody diluted in staining buffer (PBS with 1% bovine serum albumin (BSA) (Sigma-Aldrich)) was performed overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The freshly isolated vessels were fixed with 3.7% PFA and incubated with mixed primary antibodies of anti-α-SMA (1: 300, A2547, Sigma-Aldrich) and anti-CYP4A (1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Blocking with 5% BSA in PBS for 1 hour at 37°C and incubation with an anti single-stranded DNA antibody (Sigma, MAB3034) diluted 1:80 in 5% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C in anti-NeuN primary antibody (1:5000; monoclonal-Mouse Cat# MAB377; Millipore, California USA) in PBSTx with 1% normal goat serum (NGS ...
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bioRxiv - Cancer Biology 2022Quote: ... and cells were then incubated for 90 minutes at RT with 0.1% Triton X-100 containing Alexa Fluor 647-conjugated anti-puromycin antibody (1:5000; Millipore, MABE343-AF647). Cells were washed thrice with PBST for 5 minutes each ...
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bioRxiv - Biophysics 2022Quote: ... cells were permeabilized with 0.3% Triton X-100 in PBS and then incubated with anti-α-actinin mouse monoclonal antibody (1:100, A7732, Sigma-Aldrich) in CAS-Block reagent (Life Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... or a concentration of 50 nM for IMAC-purified DARPins) binding to CagI was performed using a mouse-anti-FLAG M2 monoclonal antibody (dilution 1:5000; Sigma, F1804) as primary and a goat-anti-mouse antibody conjugated to an alkaline phosphatase (dilution 1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were washed as before and incubated with an HRP-conjugated anti-FLAG antibody (clone M2, Sigma-Aldrich, 1:10.000 dilution) for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... was used as a substrate for in situ hybridization and immunolabeling was carried out with primary antibodies anti-GABA (1:500, Sigma–Aldrich), anti-GFP (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were subjected to the following primary antibodies overnight at 4°C according to the experiment: mouse monoclonal anti-β-tubulin III (1:5000; Sigma) or mouse monoclonal anti-actin (1:4000 ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 μL time points were taken and quenched with 2 mM EDTA then brought to 20 μL final volume for detection using 1:500 dilution anti-H3K4me3 antibody (05-745R, EMD Millipore), 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082) ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were incubated with the corresponding primary antibodies diluted in blocking solution: anti-tyrosine hydroxylase (TH) (Millipore, #MAB318; 1:2000) or anti-NeuN (Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... Probed membrane was washed with TBS-T thrice before incubating with alkaline phosphatase-conjugated anti-mouse secondary antibody (1:10000 dilution, Sigma, USA) or goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... for one hour before overnight incubation at 4℃ with one of the following primary antibodies: anti-NG2 (1:200; Millipore AB5320), anti-Laminin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... For staining the following primary antibodies were used: For labeling of nerve fibers mouse anti-beta-Tubulin III (1:200, Sigma, T8578); for pre-synaptic terminal staining rabbit anti-Synapsin ...
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bioRxiv - Neuroscience 2024Quote: ... iBECs were cultured in Transwell inserts with 3.0 µm pores and exposed to FITC-conjugated 150 kDa dextran (0.5 mg/ml) or anti-TDP-43 antibody (1 µM, Sigma, Cat#T1705) and 10 µl of phospholipid-shelled microbubbles with octafluoropropane gas core prepared in-house as described in (40) ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 and 10% foetal calf serum in PBS] containing primary antibodies including: mouse anti-NeuN (1:200; Millipore, #MAB337); mouse anti-parvalbumin (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were washed five times with five-minute incubations in PBST and then incubated overnight in primary antibody solution containing anti-acetylated alpha-tubulin (1:500; Sigma, T6793) in PBST plus 20% NGS ...
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bioRxiv - Microbiology 2024Quote: ... and treated with 16 μM Hoechst 33342 and/or Anti-Human IgG (Fc specific)−FITC antibody (1:100 dilution, Sigma-Aldrich) in iCM ...
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bioRxiv - Immunology 2024Quote: ... Viral nucleoprotein (NP) was detected by ELISA as described using a 1/1,000 dilution of the anti-NP antibody (Millipore cat. #MAB8257) and a 1/2,000 dilution of the horseradish peroxidase-labeled goat anti-mouse IgG (SeraCare cat ...
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bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and the indicated proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Developmental Biology 2023Quote: ... the membrane was incubated for 1h at room temperature with the secondary antibody anti-mouse IgG peroxidase-conjugated (1/500; A5906, Sigma-Aldrich). The revelation was performed using Pierce™ ECL Plus Western Blotting Substrate (32312 ...