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Citations for Millipore Sigma :
8951 - 9000 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Blood was extracted from hBLT mice at 3 and 5-6 weeks post-infection and lysed with Red Blood Cell Lysis Buffer (SIGMA). T cells were activated for 1.5 h with 5μl/ml of anti-CD28 and anti-CD49d in the presence or absence of 6.4μg/ml of a Gag pool of peptides in the presence of 0.5μg/ml Brefeldin A ...
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bioRxiv - Immunology 2021Quote: ... 1-2 x 106 mononuclear cells isolated from blood or tonsil samples were cultured in a round bottom 96 well plate at 37°C 5% CO2 in R10 media for 6 hours with or without 200 ng/ml phorbol myristate acetate (PMA) and 1.0 μg/ml Ionomycin (Sigma-Aldrich) or 0.5 μM/ml 5-amino-6-D-ribitylaminouracil (5-A-RU ...
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bioRxiv - Molecular Biology 2021Quote: ... 4×106 ESCs were allowed to attach to gelatinised 15cm dishes for 6-8 hours and treated with 1μM all-trans retinoic acid (Sigma-Aldrich) in EC-10 medium (DMEM supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2019Quote: ... adult animals (6-12 weeks) were perfused with 25 mL of PBS (Hyclone) and 25 mL of 4% paraformaldehyde (PFA, Sigma) sequentially at room temperature (RT) ...
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bioRxiv - Neuroscience 2019Quote: ... mononuclear cells (1×106 cells/mL) were cultured for 6 h with or without phorbol 12-myristate 13-acetate (PMA, 50ng/mL; Sigma), ionomycin (50ng/mL ...
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bioRxiv - Biophysics 2019Quote: ... The reaction was started by adding 20 µl of 500 µM DHF (Sigma Aldrich, cat# D7006, CAS: 4033-27-6) in MTEN with 5 mM DTT to 180 µL of pre-reaction mixture ...
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bioRxiv - Cell Biology 2019Quote: ... Covalently modified αSYN was separated from non-reacted dye on a Sephadex G-25 column (Amersham, GE) and concentrated with 6 kDa MW cut-off spin columns (Millipore). The final concentration of the fluorescently labeled protein was measured by UV-VIS spectrophotometry at 280 nm with E ...
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bioRxiv - Bioengineering 2019Quote: Cy3-(GT)6-SWCNTs and Cy3-(GT)30-SWCNTs were first filtered 3 times using 100kDa Amicon centrifuge filters (Millipore) to remove free Cy3-DNA from solution ...
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bioRxiv - Bioengineering 2019Quote: ... Laccase activity was followed by the oxidation of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (Sigma-Aldrich, USA) (33) ...
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bioRxiv - Cancer Biology 2020Quote: Cre expression and activity was induced in 6-8 week old Krt19-CreERT and Rosa26-CreERT mice by intraperitoneal injection of tamoxifen (Sigma) dissolved in corn oil (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... Viral size particles in the filtrate were concentrated to ~500 mL (viral concentrate) using tangential flow ultrafiltration with a 30kDa MW prep-scale Spiral Wound TFF-6 cartridge (Millipore) (53).
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bioRxiv - Cell Biology 2019Quote: Cold APC purification buffer (100 mM NaPi, Sigma#S3139 and Sigma#S9390, 300 mM KCl, Sigma#P9333, 5 mM MgCl2 × 6 H2O, Sigma#M2670 ...
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bioRxiv - Developmental Biology 2019Quote: ATDC5 were plated in 6-well plates to 80% confluency and lentiviral transduction was using diluted viral media with 0.1% polybrene (EMD Millipore) for 24 hours followed by selection with 4μg/ml Blastocidin and Puromycin for 5 days post transfection ...
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: Bloodstream parasites were spun down and resuspended in differentiation media (19) supplemented with 6 mM cis-aconitate (Sigma-Aldrich A3412) at 27°C for three days.
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bioRxiv - Biochemistry 2019Quote: ... 3 mM KCl) and harvested in a PBS lysis buffer (PBS plus 6 uL/mL protease inhibitors cocktail (Sigma P8340), 0.6 mM PMSF ...
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bioRxiv - Genomics 2019Quote: ... 1.5 g/L (days 0-5 and days 11-28) or 2.5 g/L (days 6-10) NaHCO3 (Sigma-Aldrich), and 0.25 mM (days 3-10 ...
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bioRxiv - Neuroscience 2019Quote: 12 female young adult C57BL/6 mice (mean age 2.2 months) were sedated intraperitoneally with chlorprothixene (0.5 mg/kg, Sigma-Aldrich, UK) and anaesthetised with isoflurane (2% for induction ...
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bioRxiv - Cancer Biology 2019Quote: ... 6 week-old mice were injected intraperitoneally three times at one day interval with tamoxifen (T5648; Sigma-Aldrich, Bornem, Belgium) dissolved in corn oil at a concentration of 30 mg/mL and at a dose of 150 mg/kg of body weight ...
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bioRxiv - Immunology 2021Quote: ... Monolayers were then rinsed thoroughly with Promocell DC generation medium to remove non-adherent cells and subsequently were cultured for 6 days in dendritic cell generation medium supplemented with cytokines (Sigma) to generate immature MoDCs.
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured for different periods (6, 12 or 24 hours) in different combinations of vascular endothelial growth factor (VEGF, human recombinant; Millipore) and brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Neuroscience 2020Quote: ... Spontaneous IPSCs were isolated by blocking AMPA receptor-mediated events with CNQX (6-cyano-7-nitroquinoxaline-2,3-dione; 20uM; Sigma-Aldrich). Miniature events were isolated by blocking sodium channels with the addition of tetrodotoxin (1uM ...
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bioRxiv - Neuroscience 2020Quote: ... two brains were pooled (n=6) and homogenized in 300 µL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). Tissue preparation and biochemical analysis followed protocols described in (Sachett et al. ...
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bioRxiv - Biophysics 2020Quote: ... Dead cells were removed on day 6 after T-cell isolation by means of density-dependent gradient centrifugation (Histopaque 1119, Sigma). Antigen-experienced T-cells were used for experiments on day 7 – 9.
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bioRxiv - Cancer Biology 2021Quote: ... cells were plated in 6-well plates and stained/fixed with 2.5mg/mL solution of crystal-violet (Sigma, Cat #C3886) in 20% methanol.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were trypsinazed and plated at a density of 25000 cells/ml in 6-multiwell plates in DMEM (Sigma, UK), supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626), 0.4 mg/L biotin and 0.5% methanol ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: Frozen skin tissues from a normal individual and the patients were sectioned at 6 μm and stained with mouse monoclonal anti-NC1 C7 antibody (clone LH 7.2; Sigma-Aldrich) at a 1:1000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... three brains were pooled (n=6) and homogenized in 450 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich) and centrifuged at 10,000 g at 4 °C in a cooling centrifuge ...
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bioRxiv - Neuroscience 2020Quote: ... We mechanically dissociated the tissue in a glass homogenizer with 6 ml of aCSF-based Homogenization buffer [aCSF plus 10 mM HEPES (Sigma) to maintain stable pH despite CO2 released from the cells ...
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bioRxiv - Cancer Biology 2020Quote: ... In some cases mononuclear cells were cultured as mammospheres in 6 cm culture plates coated with polyhydroxyethylmethacrylate (12 mg/ml, Sigma), under hypoxic conditions (7% O2 ...
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bioRxiv - Cell Biology 2021Quote: C57BL/6N female mice (4-6 weeks old) were superovulated by injection with 5 IU of pregnant mare serum gonadotropin (PMSG) (Millipore), followed 5 IU of human chorionic gonadotropin (hCG ...
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bioRxiv - Physiology 2020Quote: ... HMDC (passage 7-9) were seeded onto plastic 6-well fibronectin-coated (10 μg/ml in PBS, Sigma-Aldrich, UK) culture plates (described below ...
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bioRxiv - Molecular Biology 2020Quote: ... Nucleofected cells were plated onto duplicate wells of Matrigel®-coated 6-well culture plates for 24 h before adding 2.5μg/mL puromycin dihydrochloride (Sigma-Aldrich) for selection ...
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bioRxiv - Plant Biology 2020Quote: ... 6 to 7 day old light or dark grown seedlings were pre-treated with 50 μM cycloheximide (CHX, Sigma Aldrich) or with 50 μM proteasome inhibitor Bortezomib (Selleckchem) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The washed cell pellets were resuspended into 6 mL of DM29 with the addition of 60 μL of pefabloc (100 mM, Sigma) and 6 μL of DNase I (20 mg mL-1 ...
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bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Microbiology 2021Quote: ... Exponentially growing cells pre-cultured in the presence or absence of Mn were harvested and resuspended at a density between 1E+07 and 2.5E+07 cells/mL in their corresponding growth medium supplemented with erythromycin (5μg/mL) and 10μM 5(6)-carboxyfluorescein (Sigma-Aldrich 21877), with or without Mn (20μM) ...
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bioRxiv - Microbiology 2021Quote: ... trypsinized and analysed on a Guava EasyCyte 6/2L bench cytometer equipped with 488 nm and 532 nm lasers (Millipore), for detection of GFP-positive cells and dextran-positive cells ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mg/L avidin and 6 mM MgCl2 were added to the supernatant to improve the Strep tag binding efficiency with Strep-Tactin beads (Novagen). Next ...
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bioRxiv - Systems Biology 2020Quote: ... TMT labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (Sigma) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... parasites at 32 hpi and 38 hpi were cultured at 37 °C or 40 °C for 6 h in the presence or absence of 20 μM Wortmannin (Selleckchem, Houston, TX) or 40 μM LY294002 (Sigma). The cells were subsequently washed and resuspended in complete medium (1% hematocrit) ...
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bioRxiv - Neuroscience 2021Quote: ... Astrocyte cell line cultures were scraped off 6-well plates with ice-cold RIPA buffer with protease and phosphatase inhibitors (MSSAFE, Sigma) and transferred to a chilled microcentrifuge tube ...
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bioRxiv - Neuroscience 2021Quote: ... four brains were pooled (n=6) and homogenized in 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich) and centrifuged at 10,000 g at 4 °C in a cooling centrifuge ...
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bioRxiv - Cancer Biology 2020Quote: ... host cells were transduced with viral particles in 6-well dish along with polybrene (hexadimethrine bromide; 8μg/ml) (Sigma-Aldrich) to increase the transduction efficiency ...
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bioRxiv - Cell Biology 2021Quote: SK-N-SH-N cells were plated onto 6-well tissue culture plates coated with poly-L-lysine (Sigma-Aldrich). Cells were plated at 3×105 cells/ml in MEMα medium containing a very low-serum level (2% FBS) ...
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bioRxiv - Cell Biology 2021Quote: NCMs were seeded at a density 4 or 8 × 105 cells/well in 6-well plates coated with 0.01% of collagen (Sigma-Aldrich) and cultured in a medium containing DMEM/Medium199 (4:1) ...
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bioRxiv - Cell Biology 2022Quote: ... and 3 mL volumes of culture were transferred per well to 6-well tissue culture-treated polystyrene plates (Sigma CLS3506) and incubated at 23°C for 48 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Liposomes consisting of DPPC/egg yolk PC/egg yolk PG/cholesterol (6:3:1.2:0.95, DPPC from Avanti Polar Lipids, all other Sigma) with 0.6 μCi tracer [2-palmitoyl-1-14C]-dipalmitoyl phosphatidylcholine (14C-DPPC ...
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bioRxiv - Neuroscience 2022Quote: ... rats were subcutaneously injected with desipramine (15 mg/kg) 30 min before 6-OHDA (Sigma-Aldrich, Saint Quentin-Fallavier, France) injection in order to protect the noradrenergic neurons ...