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Citations for Millipore Sigma :
851 - 900 of 1090 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... whole cell lysates were prepared from two pellets per group using Phosphosafe Extraction Reagent (Merck Millipore, Darmstadt, Germany) containing 1 mM Pefablock SC (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: Larvae were incubated from 30hpf to 48hpf with a mix of two S-phase inhibitors: 30 mM Hydroxyurea (HU) (Sigma-Aldrich, H8627; diluted in water) and 150 μM Aphidicolin (A ...
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bioRxiv - Neuroscience 2022Quote: During the training phase for the short-term memory experiments the STROBE was loaded with 4 µL of tastant (salt: Sigma #S7653 or sucrose: Sigma #S7903) on channel 1 and 4 µL 1% agar on channel 2 ...
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bioRxiv - Biophysics 2022Quote: ... This oil was dispersed and emulsified by hand in an aqueous continuous phase containing 15% w/w of Pluronic F68 block polymer surfactant and 1% w/w sodium alginate (Ref: W201502, Sigma Aldrich, St. Louis, MO, USA) at a final oil fraction equal to 75% w/w ...
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bioRxiv - Microbiology 2020Quote: ... 100 μl of sample were added to 5PRIME Phase Lock tubes (Quantabio, Beverly, MA, USA) followed by addition of 20 μl of chloroform/tube (Sigma-Aldrich, St. Louis, MO, USA) and inversion by hand 10 times ...
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bioRxiv - Microbiology 2021Quote: ... ovatus cells were grown to mid-exponential phase on either purified mucin O-linked glycans (purified in house from Sigma Type III porcine gastric mucin) or glucose as a reference as previously described (22) ...
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bioRxiv - Bioengineering 2022Quote: ... SLS-graft-PLGA (SLS-PLGA) was dissolved in ethyl acetate (EtOAc, organic phase) and calcium peroxide (CaO2, Cat# 466271, Sigma-Aldrich, St. Louis, MO, USA) and dissolved under stirring at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Selected fractions were combined and subject to a 2D-HPLC analysis by reversed-phase HPLC (Ascentis Express RP-amide from Sigma-Aldrich; 150 × 4.6 mm, 2.7 µm) and a gradient of 30% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Collected cells were immediately spun down and resuspended in BugBuster® Protein Extraction reagent (Novagen, Merck and Co. Inc) to lyse the bacterial cells and release proteins ...
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bioRxiv - Cell Biology 2020Quote: ... The cell pellet was resuspended in 100 μl of Proteinase K extraction buffer [40 mM Tris-HCl (pH 8.0) (Sigma), 1% Tween-20 (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: RNA extraction with on-column DNAse treatment were done using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Yolk sacs were dissected from embryos and used for DNA extraction with the Red Extract-N-Amp kit (Sigma). Usp9x status was assessed by PCR using Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Microbiology 2020Quote: ... cell pellets were lysed with the M-PER Protein Extraction Reagent (Pierce) complemented with a protease inhibitor cocktail (Sigma) and a phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Plant Biology 2020Quote: ... The protein extraction buffer (50 mM TrisHCl, pH 7.0, 1% w/v SDS, P9599 protease inhibitor cocktail, Sigma-Aldrich) was added to the powder ...
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bioRxiv - Plant Biology 2021Quote: RNA from frozen ground stem samples was extracted using a Plant Total RNA extraction kit (Sigma, St Louis, MO) with modifications to the kit protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The pellets were washed with cytosol extraction buffer for three times and resuspended in membrane extraction buffer (50 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM DTT, protease inhibitor cocktail (Sigma), 0.5% NP40 ...
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bioRxiv - Plant Biology 2022Quote: ... Leaves samples were homogenized in liquid nitrogen and incubated with cytosol extraction buffer (50 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM DTT, protease inhibitor cocktail (Sigma), 0% NP-40 (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... protein induction was performed on a small scale (2mL) and cells were disrupted using BugBuster protein extraction reagent (Novagen).
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The ground tissue was resuspended in extraction buffer (50 mM Tris pH 7.5, 2% SDS and a protease inhibitor cocktail [P9599, Sigma]) and incubated for 30 minutes on ice ...
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bioRxiv - Plant Biology 2021Quote: ... resuspended in 1 mL of extraction buffer containing 10% (w/v) trichloroacetic acid (TCA; Sigma-Aldrich; St. Louis, USA) in acetone (Merck ...
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bioRxiv - Plant Biology 2021Quote: ... Total proteins were extracted in SDS extraction buffer (50 mM Tris, pH 7.8, 2 % SDS, 1 x protease inhibitor cocktail; P9599, Sigma), 2 mg/ mL NaF ...
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bioRxiv - Neuroscience 2022Quote: ... HeLa cells or iPSC-derived motor neurons grown on 6-well plates were rapidly rinsed with ice-cold PBS and subjected to ATP extraction with 0.5% TCA (Sigma). After incubation for 15 min at room temperature ...
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bioRxiv - Immunology 2022Quote: RNA extraction of freshly isolated cells in BAL or detached AMs was performed using total RNA purification kit (Sigma) together with DNase I (invitrogen ...
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bioRxiv - Systems Biology 2019Quote: ... the recovered nuclear pellet was extracted as for broccoli nuclei using Nuclear Extraction Buffer (CelLytic PN kit, Sigma-Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... Beads were removed in a magnetic field and Trizol/Chloroform RNA extraction was performed according to the manufacturer (Sigma). RNAs were precipitated using 2 volumes EtOH 100% ...
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bioRxiv - Developmental Biology 2020Quote: Genomic DNA extractions were performed with Extract-N-Amp or REDExtract-N-Amp™ Tissue PCR Kits (Sigma-Aldrich). Whole embryos or adult zebrafish fin-clips were lysed by incubation in a mixture of 50 μL extraction solution (Extraction Solution E7526 ...
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bioRxiv - Systems Biology 2019Quote: ... The digested DNA was cleaned up through phenol/chorloform extraction followed by ethanol precipitation with 20ug of glycogen (Sigma) as a carrier.
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bioRxiv - Plant Biology 2020Quote: ... 375 μL of methanol as extraction solvent and 25 μL of a 10 % (w/v) solution of canthaxanthin (Sigma) in chloroform as internal control ...
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bioRxiv - Microbiology 2021Quote: ... Cells were centrifuged and resuspended (50 µl per 1 mL of sample) with SDS sample loading buffer (msGFP2 fusions) or BugBuster Protein Extraction Reagent with 0.1% lysonase (Millipore) followed by SDS loading buffer (3xFLAG fusions) ...
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bioRxiv - Molecular Biology 2021Quote: ... Each piece of sclera was rinsed with PBS and then placed into 20 mL of pH 7.5-8.0 extraction medium containing: >1000 BAEE unit/mL trypsin (9002-07-7, Sigma-Aldrich), 4 mM ethylenediaminetraacetic acid (60-00-4 ...
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bioRxiv - Plant Biology 2020Quote: ... Leaf tissue was ground in extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.2 % Nonidet P-40 [Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extraction from skelatal muscle tissue or C2C12 mouse myoblasts was done using TRI Reagent (Millipore-Sigma #T9424). Muscle tissue samples were flash-frozen in liquid nitrogen until further processing ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extraction from skelatal muscle tissue or C2C12 mouse myoblasts was done using TRI Reagent (Millipore-Sigma #T9424). Muscle tissue samples were flash-frozen in liquid nitrogen until further processing ...
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bioRxiv - Cell Biology 2020Quote: HMLE variants were washed with PBS prior to protein extraction with lysis buffer (PBS, protease inhibitor cocktail (Sigma-Aldrich), phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Evolutionary Biology 2019Quote: DNA was extracted from a single GRZ male individual’s muscle tissue by griding in liquid nitrogen followed by phenol-chlorofom extraction (Sigma). The rapid sequencing kit (SQK-RAD004 ...
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bioRxiv - Immunology 2020Quote: For immunoblot analysis, the M-PER Protein Extraction Reagent (Pierce, USA) supplemented with a protease inhibitor cocktail (Sigma, USA) was used to lyse the cells ...
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bioRxiv - Plant Biology 2023Quote: RNA extraction of Marchantia polymorpha and Physcomitrium patens was done using the Spectrum™ Plant Total RNA Kit (Sigma) and for Arabidopsis thaliana the NucleoSpin RNA Plant min kit (Macherey -Nagel ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells (100% confluent) were harvested and lysates prepared using CytoBuster protein extraction reagent (Novagen/EMD, Gibbstown, NJ) supplemented with complete mini EDTA-free protease inhibitor (Roche ...
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bioRxiv - Plant Biology 2023Quote: Frozen leaf tissue was ground in two volumes of protein extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.1% Nonidet P-40 [Sigma-Aldrich] ...
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bioRxiv - Genomics 2023Quote: ... the PFC was placed in 5 ml ice-cold nuclei extraction buffer (0.32 M sucrose (Sigma-Aldrich, S0389-500G), 5 mM CaCl2(Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... the nuclei enrichment and isolation was performed on fresh leaf material using CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich). DNA was extracted from plant cells and Hi-C libraries were prepared using EpiTect Hi-C Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... followed by resuspension of the dry pellets by vortexing (≈1 min) and subsequent addition of 150 μL lysis buffer (HEPES buffer containing 0.35X BugBuster Protein Extraction Reagent (Novagen) and 0.1% Lysonase Bioprocessing Reagent (Novagen)) ...