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Citations for Millipore Sigma :
551 - 600 of 1090 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The mobile phase buffer consisted of 150 mM AMAC in water and filtered using a 0.22 µm disposable membrane cartridge (Millipore) before use ...
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bioRxiv - Microbiology 2021Quote: ... in the logarithmic phase of growth were plated onto chamber Lab Tek culture slides in 2.5 mL of RPMI 1640 (Sigma), 10% FBS medium containing 25 ng/mL of phorbol 12-myristate 13-acetate (Sigma ...
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bioRxiv - Biochemistry 2022Quote: The enriched biomass in the liquid phase was filtered on a 0.22-μm pore size polycarbonate membrane (Millipore, USA) and a half was used for DNA extraction and the other half for RNA extraction ...
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bioRxiv - Immunology 2022Quote: ... The aqueous phase was transferred to a new tube and 0.6 mL of isopropyl alcohol (Sigma Aldrich, Darmstadt, Germany) was added per mL TRIzol ...
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bioRxiv - Microbiology 2022Quote: ... Log-phase bacteria were resuspended in RPMI 1640 infection media: supplemented with 10% human serum (Millipore Sigma, Burlington, MA), 10 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... 10uL of sample was spiked with 10uL of internal standard (IS; L-Trp-d5) and 10uL acidified mobile phase (AMP; 0.2%FA/0.05%TFA/1% acetonitrile (ACN; Millipore-Sigma) in water) ...
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bioRxiv - Genomics 2023Quote: ... The second barrel was filled with 5 μl of the organic phase mixture of 10 mM THATPBCl in 1,2 DCE (Sigma), following silanisation of the backend of the nanopipette by exposure to Trichloro(1H ...
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bioRxiv - Molecular Biology 2023Quote: ... The aqueous phase (400-500 µL) was added to 300 µL of pre-cooled isopropanol/70% ethanol (Sigma, USA), and immediately mixed via pipetting up and down 7-8 times ...
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bioRxiv - Cell Biology 2023Quote: ... it was baked at 150°C for 2 hours and coated with gas-phase trichloro(perfluorooctyl)silane (Sigma-Aldrich). A negative mold of the silicon wafer was created using PDMS and treated with silane in the same way as the wafer ...
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bioRxiv - Molecular Biology 2023Quote: ... Metacyclic promastigotes were purified from stationary phase promastigote culture using agglutination with peanut lectin from Arachis hypogaea (Sigma-Aldrich), as previously described [4].
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bioRxiv - Cancer Biology 2023Quote: LC-MS/MS analysis was performed with ion-pairing reverse phase chromatography using an Ascentis Express column (C18, 5 cm × 2.1 mm, 2.7 μm, Sigma-Aldrich) for separation and a Waters Xevo TQ-XS triple quadrupole mass spectrometer ...
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bioRxiv - Cell Biology 2024Quote: ... The dried samples were resuspended in 50 μl of LC/MS phase A containing an internal standard (Leu-Tyr, 0.02 mg/mL, SIGMA). Quality control (QC ...
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bioRxiv - Systems Biology 2023Quote: ... Mobile phase A and B were 10 mM ammonium formate (Sigma-Aldrich, >99/0%, LC-MS grade, 70221-100GF), pH 10 and 20% 10mM ammonium formate (pH 10)/80% methanol (Optima LC/MS grade ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... log-phase cells were washed with lithium acetate (100 mM) and incubated in a mix containing lithium acetate (Sigma), PEG 2000 (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... Cells grown in the log-phase were harvested and resuspended to 2.5 × 106 cells/ml in Dulbecco’s PBS (Sigma-Aldrich) for tumour injection.
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bioRxiv - Microbiology 2024Quote: ... Equal cell numbers of mid-log phase p25.C2 and AMD290 were conjugated for 6 hours on 0.45 μm nitrocellulose filters (Millipore) overlaid on LB agar plates containing Diaminopimelic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... The upper aqueous phase was washed once with an equal volume of chloroform:isoamyl alcohol (24:1, v/v) (Sigma-Aldrich) by vigorous shaking and centrifugation at 12,000g for 15min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... mid-log phase cells expressing pHlourin were permeabilized with digitonin at 100 μg/ml final concentration (Sigma-Aldrich, Cat #D141) for 10 minutes at 30C ...
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bioRxiv - Microbiology 2019Quote: Cell wall integrity and oxidative stress assays were carried out with cultures grown at 28 °C to exponential phase in CMD and spotted at 0.4 OD600 in CM plates supplemented with calcofluor white 40 µg/ml (Sigma-Aldrich), Congo Red 50 µg/ml (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... Cells were collected at different time-points of phase two growth to monitor erythroid differentiation and extract RNA using Tri-reagent (Sigma). Differentiation was assessed using qRT-PCR (with Taqman probes for HBB and HBG2 ...
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bioRxiv - Microbiology 2019Quote: ... Microaerobic cultures were inoculated at an OD660nm of 0.1 from stationary-phase precultures in 50 mL Bio-One Cellstar Cellreactor tubes (Sigma-Aldrich) containing 30 mL of the same medium ...
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bioRxiv - Plant Biology 2020Quote: ... The combined organic phase was dried using CentriVap Cold Traps (Labconco) and derivatized using bis-(N,N,-trimethylsilyl)-tri-fluoroacetamide (BSTFA; Sigma) as described previously (Franke et al. ...
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bioRxiv - Microbiology 2021Quote: ... 450 μl of aqueous phase was collected and precipitated with 1 ml 100 % ethanol in the presence of 45 μl NaOAc (Sigma) and 1.2 μl of Glycoblue (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... These strains were grown in complete 7H9 medium supplemented with hygromycin until mid-log phase and expression of recombineering enzymes was induced by 0.2% acetamide (Sigma-Aldrich) overnight at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... A549 LIMD1−/− and LIMD1+/+ were grown until reaching the beginning of the exponential growth phase and then detached and resuspended in Matrigel 5mg/ml (Sigma). 1×106 cells in 100 μl of Matrigel were injected subcutaneously into the mice ...
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bioRxiv - Biophysics 2020Quote: ... Warner Instruments) were either surface functionalized using plasma cleaning for activation followed by vapor phase silanization with Dichlorodimethylsilane (Sigma-Aldrich), resulting in a hydrophobic surface for sandwiching the curing hydrogel or with 3-(Trimethoxysilyl)propylmethacrylate (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... A 25 μL aliquot of the quenched reaction mixture was prepared for MALDI-TOF mass spectrometry with a C18 reverse-phase ZipTip (Millipore) following the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2021Quote: Interactions of shikonin with cell sap was analyzed by normal phase TLC (silica gel 60 F254, Merck Millipore, Darmstadt, Germany). In addition ...
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bioRxiv - Microbiology 2021Quote: ... aureus Cowan I wildtype as well as the isogenic mutants ΔpknB and Δstp were grown to logarithmic phase and 108 CFUs mixed 1:1 with cytodex beads (Sigma) 60 ...
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bioRxiv - Molecular Biology 2020Quote: ... The aqueous phase was transferred to a new tube and 500 μL of ice-cold isopropanol (Sigma-Aldrich, 278475-1L) and 2 μL of Glycoblue (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... using a Waters Alliance 2695 HPLC with a C18 reversed-phase column (5 μm, 15 cm x 4.6 mm inside diameter, SUPELCOSIL LC-18-S; Sigma Aldrich) equipped with a guard column at a flow rate of 1 ml/min ...
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bioRxiv - Bioengineering 2021Quote: ... the oil phase consisted of 2.2% Ionic Krytox 157 FS-H in HFE7500 and the aqueous phase consisted of 40 μM DFHBI (Sigma-Aldrich) with or without 50 nM Broccoli aptamer in binding buffer (40 mM Tris HCl ...
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bioRxiv - Neuroscience 2019Quote: ... Chromatographic separation was done on a reversed-phase Kromasil C18 column (250 mm× 4.6 mm, 5 μm particle size) purchased from Sigma-Aldrich. The Shimadzu Nexera system was equipped with dual pumps ...
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bioRxiv - Systems Biology 2020Quote: ... Total proteins from diverse organelles were extracted using a phase-transfer surfactant solution containing 12 mM sodium deoxycholate (Sigma–Aldrich), 12 mM sodium lauroyl sarcosine (MP Biomedical ...
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bioRxiv - Biophysics 2019Quote: ... coli imp4213 cells were grown to early stationary phase (OD600 = 0.8) and incubated in media containing 1 μg mL−1 vancomycin (Sigma-Aldrich) for 10 min at 37 °C before fixation ...
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bioRxiv - Plant Biology 2020Quote: ... a binary gradient was used, with mobile phase A consisting of water (Tedia, Fairfield, Ohio, USA) and 0.1% formic acid (Sigma-Aldrich), and mobile phase B consisting of acetonitrile (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... mobile phase and stained using ethanol: sulfuric acid (70:3 v/v) solution containing 1% (w/v) orcinol monohydrate (Sigma). HPAEC-PAD samples were diluted to 50 μM and resolved on a Dionex PA20 column in a mobile phase of 30 mM NaOH and gradient of 10 mM – 120 mM sodium acetate over 40 min at a flow rate of 0.5 mL min-1 ...
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bioRxiv - Bioengineering 2019Quote: ... The 20-µL aqueous phase contained 1×WarmStart LAMP Mastermix and 50 μM calcein (Sigma-Aldrich, St. Louis, MO, USA). Four parameters including oil phase volume ...
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bioRxiv - Plant Biology 2019Quote: ... The dried non-polar phase was resuspended in 70 μL of pyridine and derivatized with 30 μL of MTBSTFA + 0.01 % t-BDMCS (Sigma-Aldrich). Following transfer to a glass vial ...
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bioRxiv - Plant Biology 2021Quote: ... and mobile phases A and B consisted of water (Tedia, Fairfield, USA) and 0.1% formic acid and acetonitrile (Sigma-Aldrich) and 0.1% formic acid ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µL of stationary phase cultures of the complemented strains were spotted in BHI agar plates supplemented with Congo Red (Sigma) 25 µg.mL-1 and IPTG 1 mM ...
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bioRxiv - Cell Biology 2022Quote: ... diluted with 150 μl of 1% trifluoroacetic acid (TFA) and desalted on poly(styrenedivinylbenzene)-reversed phase support (SDB-RPS) micro-columns (Sigma) as described previously(Humphrey et al. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cells grown to mid-log phase were fixed and stained using 1 part Lugol’s solution (EMD Millipore Cat. No. 1.09261.1000) with 3 parts culture (usually 25 µl and 75 µl) ...
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bioRxiv - Microbiology 2022Quote: ... during the exponential growth phase after adding 100 μM of the PSII blocker 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU, Sigma-Aldrich, USA).
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bioRxiv - Neuroscience 2022Quote: ... Phase I: Mice in the experimental group (“OXY mice”) were supplied with a single bottle of oxycodone hydrochloride (Sigma Aldrich) dissolved in drinking water as their only source of liquid ...
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bioRxiv - Cell Biology 2022Quote: ... cells were synchronised in early S phase by two consecutive rounds of treatment with 2 mM thymidine and 3 ug/ml (Sigma) aphidicolin ...
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bioRxiv - Molecular Biology 2023Quote: Yp strains were induced to upregulate expression of the T3SS and associated effectors by taking 100 μL of stationary phase overnight cultures and adding to 3 mL 2x YT media containing 20mM sodium oxalate (Sigma) and 20mM MgCl (Sigma) ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were expressed by induction at exponential growth phase (OD600nm≈0.5) with 0.5 mM isopropyl-β-D-thiogalactopyranoside (Sigma, I6758) for 2.5 h at 37°C ...
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bioRxiv - Genomics 2023Quote: ... the aqueous phase was passed through a 0.5-ml Amicon Ultra Centrifugal Filter with a 100 kDa cutoff (Millipore, UFC510024) by 3 min of centrifugation at 14,000g ...
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bioRxiv - Microbiology 2023Quote: Bacteria from synchronized log-phase cultures (OD600 = 0.3) were incubated in LB buffered medium supplemented or not (control) with 1 mM Paraquat (Sigma Aldrich) for 30 min at 37 °C in a 12-well polystyrene plate ...