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Citations for Millipore Sigma :
851 - 900 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... followed by incubation with fluorescent-tagged carboxylate-coated 2 um size latex beads (L3030, Sigma) for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-tagged Pros-S-HD in soluble fraction was purified using Glutathione-Agarose beads (Sigma). The bound proteins were eluted in elution buffer (50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
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bioRxiv - Genetics 2022Quote: V5H6-tagged FRQ was immunoprecipitated with 20 μL of V5 agarose (Sigma-Aldrich, Catalog #7345) from 2 mg of centrifugation-cleared lysate ...
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bioRxiv - Microbiology 2022Quote: ... and FLAG tagged S-protein was stained using anti-FLAG (clone M2, Sigma-Aldrich, F1804). SARS-CoV-2 Spike Antibody (ProSci ...
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bioRxiv - Neuroscience 2023Quote: ... GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich. 200 µg of GST-α synuclein was incubated with the equivalent amount of purified His-spectrin at 4°C overnight in the presence of glutathione sepharose beads ...
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bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...
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bioRxiv - Molecular Biology 2023Quote: ... myc-tagged TEV protease was detected with a mouse monoclonal anti-myc antibody (Sigma M4439). The proteins were detected using SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).
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bioRxiv - Cell Biology 2023Quote: ... Flag-tagged Scube was detected by using polyclonal α-FLAG antibodies (Sigma, St. Louis, USA). GAPDH ...
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bioRxiv - Immunology 2023Quote: ... Co-IP of FLAG-tagged proteins was performed by incubating M2 anti-FLAG resin (Sigma) with clarified cell lysates for 2 h at 4°C with end-over-end rotation ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD3 and PFD5 were detected using anti-FLAG rabbit antibody (1:5,000, Sigma), FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... 4% 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS, Sigma)] ...
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bioRxiv - Neuroscience 2022Quote: ... 3 3’-diaminobenzidine tetrahydrochloride hydrate (DAB) (Sigma SIG-32750) in sodium acetate ...
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bioRxiv - Bioengineering 2021Quote: ... 3- [(3- Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS, Sigma RES1300C), digitonin (Sigma D141) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 3-3’ diaminobenzidine tetrahydrochloride (DAB, Sigma Aldrich) staining ...
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bioRxiv - Microbiology 2022Quote: ... 3-amino-9-ethylcarbazol (3-AEC; Sigma Aldrich, Germany) was used as a chromogen ...
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bioRxiv - Cancer Biology 2019Quote: ... 14-3-3 Theta/Tau (Millipore Sigma T5942-.1MG). After exposure to the matching HRP-conjugated secondary antibody ...
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bioRxiv - Microbiology 2021Quote: ... 3-Amino-1,2,4-triazole (3-AT, A8056, Sigma Aldrich). Bovine liver catalase (C1345 ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µM CHIR99021/GSK-3 Inhibitor (Millipore Sigma 361571), and 1 µM PD0325901/MEK1/2 Inhibitor (Millipore Sigma 444968 ...
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bioRxiv - Microbiology 2021Quote: ... 3-(3-hydroxy-phenyl)propionate (Sigma Cat Number PH011597) and 3-hydroxycinnamic acid (CAS Number 14755-02-3) ...
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bioRxiv - Cancer Biology 2023Quote: ... Interleukin-3 (IL-3, Cat# SRP4135-10UG, Sigma, USA), Alexa Fluor 488 EGF complex (Cat# E13345 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 0.5 nM neurotrophin-3 (NT-3, Sigma #SRP6007). Cells were released by trituration through fire-polished glass pipettes of decreasing diameter ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/ml 3′,3′-diaminobenzidine tetrahydrochloride hydrate (Sigma), 24 units/ml catalase from bovine liver (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... and activating transcription factor 3 (ATF-3, HPA001562, Millipore). After three washes with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... 0.05% 3-3′ diaminobenzidine tetrahydrochloride hydrate (DAB, Sigma D5637) and 4% sucrose in PBS ...
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bioRxiv - Developmental Biology 2021Quote: Embryos used for long-term imaging or imaging after 1 dpf were treated with 0.003% 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) to prevent melanin pigment formation.
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bioRxiv - Molecular Biology 2019Quote: ... The PCR of tetur01g11270 fragments (amplicon 1 and 2) was conducted using the Expand™ Long Range dNTPack (Sigma-Aldrich). PCR reaction mixtures were prepared according to the manufacturer’s instructions and using the following temperature profile ...
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bioRxiv - Genomics 2019Quote: ... was used along with 0.36 μl of 50 μM custom-designed SYNE2 short and long isoform primer and 0.5 μl of 10 μM probe set (Table 1, obtained from Sigma-Aldrich) and 1 μl of the primer limited cyclophilin A (PPIA ...
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bioRxiv - Biophysics 2020Quote: ... while recombinant PD-L1 (18 – 134 aa) and PD-L1-Long (19 – 238 aa) were cloned into pET-21b and pET-28a (Novagen), respectively ...
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bioRxiv - Microbiology 2021Quote: ... The system was fitted with a 1.5 m long 80/100 Porapak Q column (inner diameter 2.1 mm; Sigma-Aldrich, US) held at 250°C using nitrogen (50 mL/min ...
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bioRxiv - Immunology 2022Quote: ... the colon was washed with cold PBS and minced into 0.5cm long pieces before incubation in chelating buffer Hanks’ Balanced Salt Solution (HBSS) (Sigma Aldrich) with 5% foetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... and treated with a short (30 minutes) or a long (24 hours) pulse of 10 μM BrdU (Sigma-Aldrich, B5002), 24-hour post-plating ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Cytidine 3′, 5′ cyclic monophosphate (3′, 5′ cCMP) and Cytidine 2′, 3′ cyclic monophosphate (2′, 3′ cCMP) were purchased from Sigma-Aldrich (Bangalore, India) and used without further purification ...
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bioRxiv - Cancer Biology 2020Quote: ... On this plasmid a single primer PCR with primer 5’-GCA AGC CCT GAA AGC GCA AG-3’ and the cDNA human HIF-1α in a p3XFLAG-CMV™-10 expression vector (Sigma, St Louis, MO, USA) (Gort et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human mesenchymal stem cells (MSCs) were cultured on gelatin coated flasks for 2-3 days in Alpha-MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Recombinant GST-p50 subunit D434-969 was purchased from Sigma-Aldrich. Binding reactions were performed for 20 minutes at room temperature with 20 pmol annealed primers ...
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bioRxiv - Immunology 2021Quote: Recombinant cytokines were purchased from indicated suppliers: IFN-β1a (Sigma, #IF014); IFN-γ (Sigma ...
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bioRxiv - Genomics 2020Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... Following 12 h recombinant expression in Rosetta Competent Cells (Millipore, #70954) at 12° C with ampicillin and chloramphenicol selection ...
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bioRxiv - Cell Biology 2019Quote: ... 1000 U/mL mouse recombinant Leukemia inhibitory factor (LIF, ESG1107, Millipore) and Penicillin-Streptomycin-Neomycin Antibiotic Mixture (15640055 ...
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bioRxiv - Biophysics 2021Quote: Recombinant HSA expressed in Pichia pastoris was purchased from Sigma-Aldrich (#A7736 ...
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bioRxiv - Neuroscience 2021Quote: ... and with or without 0.3 µM recombinant BirA (Sigma-Aldrich; SRP0417) (Howarth and Ting ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...