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Citations for Millipore Sigma :
1101 - 1150 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... media supplemented with 1000U/mL ESGRO Recombinant Mouse LIF (Millipore Sigma, ESG1107), 2 mM GlutaMAX (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG ...
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bioRxiv - Immunology 2023Quote: ... then tested against recombinant proteins coated in Dulbecco’s PBS (DPBS, Sigma-Aldrich) overnight onto 96-well Immunlon-2 plates (VWR Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and with recombinant mouse Leukemia Inhibitory Factor (LIF, 20 U/ml, Millipore).
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bioRxiv - Biochemistry 2023Quote: ... They were incubated with recombinant ULK1 proteins (Sigma, #SRP5096-10UG, Darmstadt, Germany) in the kinase buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 10 μg/μl recombinant mouse leukemia inhibitory factor (LIF; Sigma-Aldrich) in a 5% CO2 incubator at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... interleukin 1b (recombinant murine Il-1b, Sigma-Aldrich, #I5271, 10 ng/mL) and interferone γ (recombinant mouse INFγ ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA was digested by adding 10 U DNase I recombinant (Sigma) and incubating for one additional hour ...
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bioRxiv - Cell Biology 2019Quote: ... (TDP43: 5’-gcaaagccaagaugagccu-3’ and EGFP control: 5’-gcaccaucuucuucaagga-3’; Sigma). Silenced cells were collected by trypsinization ...
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bioRxiv - Cancer Biology 2020Quote: ... The antibody reaction was visualized with 3-3’ diaminobenzidine (Sigma, D8001) followed by counterstaining with hematoxylin ...
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bioRxiv - Neuroscience 2022Quote: ... prior to being rinsed and reacted using 3-3’diaminobenzidine (Sigma) as chromagen ...
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bioRxiv - Biochemistry 2021Quote: ... and 3’,3’c-di-AMP were purchased from Sigma-Aldrich, 2’,3’-cGAMP ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimidehydrochloride (EDC) were procured from Sigma Aldrich, USA.
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bioRxiv - Physiology 2019Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma-Aldrich, No. E7750) and an amide ...
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bioRxiv - Immunology 2019Quote: ... 3 ml of 3% bacteria-free thioglycollate solution (Sigma, B2551-500G) was injected into the abdominal cavity of C57/BL6 mice ...
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bioRxiv - Plant Biology 2022Quote: ... and crosslinked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma). Biotin-labelled probes were hybridized with sRNAs on the nylon membrane and stabilized streptavidin-horseradish peroxidase was used to detect the biotin signal.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1.5 mM 3-amino-1,2,4-triazol (3-AT, Sigma). Selection plates were left for 8 days at 30°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... activated with 250mM 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma)/ N-Hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2023Quote: ... 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate hydrate (CHAPS) (C3023; Sigma Aldrich), and sodium dodecyl sulfate (SDS ...
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bioRxiv - Systems Biology 2024Quote: ... or 3-bromopyruvate (3-BP; Sigma-Aldrich, St. Louis, MO; 16490) were added to their respective wells ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Microbiology 2020Quote: ... anti-mouse (BioSupplies) or anti-His (CBM) secondary antibodies conjugated to alkaline phosphatase (Sigma Aldrich) as described in Pedersen et al35 ...
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bioRxiv - Bioengineering 2019Quote: Ni-NTA columns were prepared by packing 1 mL Ni-NTA His•Bind resins (Sigma) into a 6-ml ISOLUTE® Single Fritted Reservoir column with 10 μm polyethylene frit (Biotage ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was cleaved by overnight incubation with thrombin (from bovine plasma, Sigma Aldrich) at 4 °C during dialysis ...
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bioRxiv - Biochemistry 2020Quote: ... His-PBP1B containing fractions were pooled and treated with 2 U/mL of thrombin (Novagen) for 20 h at 4 °C during dialysis against dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... and 100 µL of alkaline phosphatase-conjugated anti-HIS tag antibody (Sigma-Aldrich, cat: A5588), diluted 1:2,000 in TBST ...
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bioRxiv - Microbiology 2021Quote: ... PfArlH or biotinylated PfArlI were subsequently mixed with HIS-Select® Nickel Affinity resin (SIGMA) while lysate of cells overexpressing Strep-SaArlI was mixed with Streptactin column material (IBA GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... DTT was added to a final concentration of 0.5 mM and His-SUMO protease (Sigma) was added to a concentration 100-fold lower than the total protein concentration ...
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bioRxiv - Bioengineering 2021Quote: ... then probed with the conjugated anti-6x His tag® mouse monoclonal antibody (Sigma-Aldrich) overnight at 4ºC or 1 hour at room temperature and rewashed three times with washing buffer (CMT ...
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bioRxiv - Physiology 2020Quote: ... the N-terminal-His-tag was labeled with the Ni-NTA Atto550 fluorophore (Sigma-Aldrich) following the protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were subjected to purification using either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... and purified with a commercial kit according to manufacturer’s instructions (His-bind buffer kit, Millipore).
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bioRxiv - Cell Biology 2021Quote: ... and a western blot was performed using anti-HIS (Sigma H-1029, mouse, 1:3000) and anti-HA (Biolegend MMS-101R or ...
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bioRxiv - Immunology 2022Quote: ... coli and protein was isolated and purified on His-binding nickel resin (Novagen, Madison, Wisconsin). Nitrophenyl-haptenated ovalbumin (NP-OVA ...
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bioRxiv - Cell Biology 2024Quote: ... containing HI-2 at final concentration of 100 µM (or vehicle control DMSO, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... His-OGT-Flag was generated by subcloning the OGT cDNA into pET30a+ vector (Novagen®). siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic® ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysate was then incubated with HIS-select HF Nickel Affinity Gel (Millipore-Sigma) overnight ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysate was then incubated with HIS-select HF Nickel Affinity Gel (Millipore-Sigma) overnight ...
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bioRxiv - Biochemistry 2023Quote: ... and His-AC elution buffer were supplemented with 0.1 v/v% Tween-20 (Sigma Aldrich), 0.1 v/v% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Biochemistry 2023Quote: Strep-Full-length REC114 and His-MEI41-127 were co-expressed from pRSFDuet-1 (Novagen) and pProEXHTb (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... For super-shift assays monoclonal 0.2 μl of Anti-His antibody (H1029, Sigma-Aldrich, USA) was used ...
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bioRxiv - Plant Biology 2023Quote: ... with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore) following manufacturer’s recommendations.
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bioRxiv - Biochemistry 2022Quote: ... and the supernatant was purified by affinity chromatography using Ni-NTA His-bind resin (Novagen). The column packed with the resin was equilibrated with binding buffer ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.4 and then bound to poly-prep chromatography columns containing His-bind resin (Novagen). The yeast lysate mixture was loaded in the columns ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% v/v heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich, F7524), Penicillin-Streptomycin 100U/ml (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 1 µg/mL His-EqtII-EGFP and DAPI (D9542, SIGMA) at room temperature for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated for 1 hour with HIS-Select® Nickel Affinity Gel (Sigma), 300 μL of gel per 500 mL of His6-SUMO-AGARP expression cell culture ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... plants grown under a long day photoperiod (16 h light/8 h dark, 60% humidity) using the SpectrumTM Total RNA extraction kit (Sigma-Aldrich, USA). The sample was enriched for mRNA using the Poly(A ...
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bioRxiv - Molecular Biology 2019Quote: ... that amplify the coding sequence of the phytoene desaturase gene (tetur01g11270) (Table S1. PCRs were performed using the Expand Long Range dNTP Pack (Roche/Sigma-Aldrich, Belgium). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... through a long-pass filter (Y-52; AGC Techno Glass, Japan) and a heat-absorbing filter (HAF-50S-50H; SIGMA KOKI, Japan). The light-induced pH changes were measured using a pH electrode (9618S-10D ...