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Citations for Millipore Sigma :
851 - 900 of 1654 citations for Real Time Thermal Cycler Thermocycler Accessories since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... For all ChIP experiments a mock IP and an experimental IP were set up at the same time using 1,350 µl pre-cleared chromatin and 10 µg of normal mouse IgG (Millipore) and monoclonal mouse anti-c-Myc respectively ...
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bioRxiv - Systems Biology 2021Quote: ... The retention times of the eluted peaks were compared to those of commercial analytical standards (Sigma-Aldrich, Madrid, Spain). Metabolite concentrations were quantified by the calibration graphs (R2 value > 0.99 ...
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bioRxiv - Neuroscience 2021Quote: ... glucose solution at 37 °C for 20 min and rinsed five times with washing buffer (PBS −/−with 0.2% fish skin gelatin (G7765, Sigma)) ...
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bioRxiv - Microbiology 2020Quote: ... Metabolite peaks were identified by their exact mass and matching retention times to those of pure standards (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... The plates were washed five times with TBS containing 0.05% v/v Tween-20 and polyclonal anti-human antibody conjugated to HRP (Sigma) added for one hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Plates were then washed 3 times with PBST before the addition of 100ul of .5mg/ml o-Phenylenediamine (Sigma) diluted in phosphate-citrate buffer with sodium perborate (Sigma) ...
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bioRxiv - Genetics 2020Quote: ... Islets were dissociated from the exocrine tissues by shaking vigorously several times followed by Histopaque 1077 (Sigma-Aldrich, 10771) density centrifugation (900g ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then washed three times with distilled water and spun down in low melting agarose 2 % (Sigma Aldrich) and solidified in ice ...
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bioRxiv - Microbiology 2019Quote: ... The antigen bound Magnetic Beads were washed five times with the lysis buffer and eluted by 2.5 volume of 3×FLAG peptide (150 ng/µl, Sigma) for 3h at 4°C with gently shaking ...
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bioRxiv - Microbiology 2019Quote: ... Larvae were washed three times in 0.05% PBS-Tween20 before incubation in 10 µg/ml Hoechst (#B2883, Sigma-Aldrich) diluted in Tris-buffered saline ...
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bioRxiv - Biophysics 2021Quote: ... the flow cell was flushed three times with 20 μL of 1 mg/mL bovine serum albumin (EMD Millipore) and incubated for 5 min to block the surface from nonspecific interactions ...
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bioRxiv - Cell Biology 2021Quote: ... twice in Tris-HCl (pH 8.5) and three times in 100 mM triethylammonium bicarbonate buffer (TEAB) pH 7.5 (Sigma-Aldrich). The washed beads were resuspended in 200 μL 50 mM ammonium bicarbonate (pH 8.3 ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed three times in PBS-gly and anti-GFP was further labelled with ATTO647N (1:500, Sigma) and anti-mCherry was labelled with CF568 (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Cryo-sections and tail skin whole mounts were washed three times in PBS and fixed in 4% Paraformaldehyde (Sigma) in PBS for 30 mints at RT ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed 3 times in PBS and blocked in PBS supplemented with 0.5% fish skin gelatin (Sigma-Aldrich) for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3 times in staining buffer (PBS with 0.1% Bovine Serum Albumin (cat. num. A8806, Sigma), 0.05% NaN3 (cat ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed five times and stained with 4',6-Diamidino-2-phenylindole (DAPI) (Sigma, Cat No. 10236276001, Germany) for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... plates were washed three times with phosphate buffered saline (PBS-Tween 20 [pH=7.4]; catalogue number 524653, Millipore Sigma), blocked with 200 μL per well casein blocking buffer (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally washed 3 times in PBS-Tween 20 0.1% and mounted in Mowiol 4-88 (81381, Sigma).
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bioRxiv - Cell Biology 2022Quote: ... the slides were washed 3 times for 5 mins while gently shaking at RT with PBS + 0.1% Tween (Sigma) and stained for 90 mins in blocking buffer with a combination of secondary antibodies ...
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bioRxiv - Systems Biology 2022Quote: ... Formic acid-extracts were spun down at 13k rpm for 10 minutes and a total of 2 μl supernatant was spotted 0.5 μl at a time near the base of a PEI-Cellulose F thin layer chromatography plate (Millipore). The spots were dried and nucleotides were resolved using freshly prepared 1.5M KH2PO4 (pH 3.4) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... DNA was purified from cells harvested at the indicated time points using a Mammalian Genomic DNA Miniprep Kit (Sigma). DNA was immobilized on nitrocellulose and analyzed by immunodot blotting with antibodies against cyclobutane pyrimidine dimers (CPDs ...
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bioRxiv - Microbiology 2022Quote: ... Nexcelom Biosciences) at the time of harvest by staining with a 0.2% final concentration of trypan blue (Sigma; T8154) in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was washed 3 times in a solution of 0.3 M guanidine hydrochloride (Sigma, Cat. No. SRE0066) in 95% ethanol ...
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bioRxiv - Neuroscience 2019Quote: ... Thereafter the sections were rinsed three times in 0.1 M sodium cacodylate buffer (pH 7.2-7.4) (Sigma-Aldrich, Germany) and incubated with 1 % osmium tetroxide (Science Services ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 times for 5 min with PBS and permeabilized for 1h at room temperature (permeabilization buffer: 2,5% donkey serum (Sigma), 1% BSA ...
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bioRxiv - Immunology 2020Quote: ... the sections were washed 3 times in PBS and permeabilized in PBS containing 0.3% Triton-X100 (#X100, Sigma-Aldrich) for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... with the exception that the samples were spiked with isotope-labelled peptides and MSRT retention time peptides standards (Sigma), prior to MS analysis.
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed three times in PBS and then fixed in 4% formaldehyde (paraformaldehyde powder, 95%, 158127-2.5KG, Sigma) at room temperature for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... Cover slides were washed three times with 0.1 M sodium cacodylate (pH 7.2) (>98% purity, Sigma Aldrich, Steinheim, Germany) and post-fixed for 1 h with 1% osmium tetroxyde in the same cacodylate buffer ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed three times in PBS with 0.05% Tween 20 (PBS-T) and then blocked with 1x casein (Sigma) for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... were collected from the top of the gradient and the fraction material was cleaned by washing three times and resuspended in 0.02 µm-filtered FSW using 100 kDa Amicon-ultra filters (Merck Millipore). Vesicles were detected in fractions with densities of 1.05–1.07 g ml−1 (fractions 3-5 from the top).
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bioRxiv - Genetics 2022Quote: ... cells were washed three times in YPD (30°C) containing 0.1 mg/ml Pronase E (Sigma-Aldrich; Catalogue # P5147), resuspended in SC minimal medium containing nocodazole (15μg/ml) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were washed five times with 100 µl/well DPBST and 50 µl/well 3,3′,5,5′-tetramethylbenzidine (TMB) substrate solution (Sigma; T0440) was added to each plate ...
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bioRxiv - Genetics 2022Quote: ... Slides were then washed three times with Tween20 in 1xPBS (0.02% final, Sigma; room temperature, 5 minutes each wash), and blocked with BSA solution (2% BSA ...
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bioRxiv - Cell Biology 2022Quote: Cells at 80–90% confluence were washed two times with minimal media [Glasgow minimal essential media (Sigma-Aldrich G6148) buffered with 10 mM HEPES] at 4 °C and then incubated with toxin (anthrax toxin ...
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bioRxiv - Developmental Biology 2022Quote: ... and spun down three times before being resuspended to a concentration of 150,000 cells/mL in 20% Optiprep (Sigma) for inDrop sorting.
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bioRxiv - Biochemistry 2022Quote: ... 10 g/L bovine serum fraction V protein and 1.5 mM β-mercaptoethanol) and disrupted by shaking five times for 1 min with glass beads (0.45–0.50 mm diameter, Sigma-Aldrich). The crude extracts were recovered by washing the glass beads four times with 5 ml TES ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed six times with PBS and incubated for 1 h with the secondary antibodies (phalloidin (Sigma - P1951), 1:500 ...
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bioRxiv - Genetics 2020Quote: ... Beads were then washed three times with lysis buffer and eluted in lysis buffer supplemented with 0.5mg/mL 3xFLAG peptide (Sigma). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Bioengineering 2019Quote: ... The filtrate was then concentrated ∼60 times using 15mL 10kDa weight cut-off Amicon Ultra Centrifugal Filters (EMD Millipore) at 3,901xg ...
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bioRxiv - Systems Biology 2019Quote: ... Beads were washed 4 times with 1 mL cold TBS pH 7.4 + 0.05% Igepal CA-630 (Sigma Aldrich Corporation). Protein was eluted with 5 units AcTEV™ Protease (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... three times for 10 minutes and then incubated with primary antibody (1:250 Rabbit anti-CGRP, Millipore-Sigma, PC205L) in blocking buffer solution (1x PBS ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... three times for 10 minutes and then incubated with primary antibody (1:250 Rabbit anti-CGRP, Millipore-Sigma, PC205L) in blocking buffer solution (1x PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were then washed 3 times with TBS containing 1% BSA and 0.1% w/v sodium azide (Sigma Aldrich). After staining ...
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bioRxiv - Neuroscience 2019Quote: ... Sections from VGLUT2-EYFP and VGLUT2-ChR2 mice were processed In parallel and for the same period of time (15 min) in 3.3’-diaminobenzidine tetrahydrochloride (DAB, Sigma fast tablets; Sigma), rinsed in KPBS ...
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bioRxiv - Cell Biology 2020Quote: ... the beads were washed with 1% Trition buffer three times and then incubated in 30 μl of 1% Trition buffer with 0.2 μg/μl of 3xFLAG peptide (Sigma) at room temperature for 20 min ...
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bioRxiv - Systems Biology 2021Quote: ... The retention times of the eluted peaks were compared to those of commercial analytical standards (Sigma-Aldrich, Madrid, Spain). Metabolite concentrations were quantified by the calibration graphs (R2 value > 0.99 ...
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bioRxiv - Genomics 2020Quote: ... 2 mM DTT) by performing buffer exchange three times using one Amicon 30 kDa MWCO spin column (Millipore UFC903008). The purified protein was then transferred to a new tube ...
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bioRxiv - Molecular Biology 2021Quote: ... A 5 µL aliquot for each time point was removed and quenched with 5 µL nuclease P1 (Sigma #N8630) in a 100 mM sodium citrate suspension (pH 4.7 ...