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Citations for Millipore Sigma :
801 - 850 of 1654 citations for Real Time Thermal Cycler Thermocycler Accessories since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured after 72hrs (or at the indicated time point) using the MTT assay (Sigma M2128), with 500ug/mL salt diluted in complete medium and incubated at 37°C for 3 hrs ...
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bioRxiv - Cancer Biology 2023Quote: Tumors were collected at different time points and enzymatically digested in 10mg.mL−1 collagenase and 0.2mg.mL−1 DNAse I (Sigma-Aldrich) for 30min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Then the wells were washed 2 times with PBS and 200 μl of SLC3A2 (1: 500) (Sigma / HPA017980) primary antibody solution (in 5%BSA ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were washed three times in PBS and incubated in DAPI solution (MBD0015, Sigma-Aldrich, Missouri, USA) to label cell nuclei ...
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bioRxiv - Cell Biology 2023Quote: ... The reaction was quenched by washing three times with the quenching buffer (containing 5 mM Trolox [Sigma-Aldrich, cat#238813-25G] ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were washed 3 times in PBS before addition of 2% Triton X– 100 (Sigma-Aldrich) PBS solution to lyse the cells for enumeration of the intracellular bacteria ...
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bioRxiv - Bioengineering 2023Quote: ... Diabetes was induced through a one-time intraperitoneal injection of 110 mg kg−1 streptozotocin (Step, Sigma Aldrich) dissolved in citrate buffer (pH 4.5 ...
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bioRxiv - Neuroscience 2023Quote: ... and retention time comparison against an in-house standard library (Sigma-Aldrich, IROA Technologies, Sea Girt, NJ, USA). Pathway analysis and analysis of metabolite set enrichment was performed using MetaboAnalyst (Xia and Wishart ...
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bioRxiv - Neuroscience 2023Quote: ... The mixtures were washed three times with 1X PBS with 0.15% Triton X-100 (Sigma, Cat#X100-500ML), and then resuspended in 20 μl of 1X NuPAGE LDS sample buffer for NuPAGE and immunoblotted analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were then washed three times with IP lysis buffer and eluted using 50 mM DTT (Sigma-Aldrich) and 0.5% SDS in lysis buffer at 58°C for 30 min ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were rinsed with 1x PBS three times and incubated with blocking buffer (0.5% BSA (Sigma-Aldrich A7030) in 1x PBS ...
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bioRxiv - Biophysics 2023Quote: ... The samples were washed five times with extraction buffer and then immunoblotted with anti-Cherry antibodies (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... cells were washed five times in ice-cold DPBS and lysed in 6 M guanidine hydrochloride (Sigma-Aldrich) in 10 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... spotted lipids were migrated three times sequentially in a running solution of 50:1 petroleum ether (Sigma Aldrich) and ethyl acetate (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... were washed three times with 3 ml of water using Amicon Ultra-4 (3 kDa cut-off, Millipore) (centrifugation at 4°C ...
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: ... brains were washed three times in PBS with 0.5% Triton X-100 (Millipore Sigma, Cat#X100, Burlington, MA) (PBST ...
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bioRxiv - Developmental Biology 2024Quote: ... PFA was removed and samples were washed 3 times with PBST (RNAse-free PBS and Tween20, Sigma Aldrich) with incubation times of 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... Animals were then harvested by washing with M9 multiple times before RNA was isolated using TriReagent (Sigma-Aldrich), column purified (Qiagen) ...
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bioRxiv - Cell Biology 2024Quote: ... then washed three times with 1xPBS and permeabilized with 0.5 % Triton-X100 (Sigma-Aldrich, St. Louis, MO, USA) in 1xPBS for another 5 min ...
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bioRxiv - Plant Biology 2020Quote: ... the epidermal strip was washed three times with Milli-Q water and stained with 50 μM H2DCFDA (Sigma-Aldrich) (10 mM stock in DMSO ...
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bioRxiv - Biophysics 2021Quote: ... cells were rinsed three times with PBS containing 0.1 g L−1 Mg2+ and 0.133 g L−1 Ca2+ (PBS++; Sigma-Aldrich) and incubated with glutaraldehyde solution (2.5% (v/v ...
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bioRxiv - Biophysics 2020Quote: ... The cells were washed three times with PBS again and incubated with Hoechst 33342 (Sigma, 14533, 10 μg/ml) for 20 min in 37 C and washed twice with PBS before acquiring the images.
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bioRxiv - Microbiology 2021Quote: ... Cells were pelleted a second time and resuspended in PBS supplemented with 2% heat-inactivated fetal bovine serum (Sigma). Numeration and viability were determined using Propidium Iodide marker exclusion and MACSQUANT Flow cytometer (Miltenyi) ...
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bioRxiv - Cell Biology 2020Quote: ... Retinal organoids (n=3-4) for each time point were homogenized using a Dounce Tissue Grinder (Sigma-Aldrich, UK) and processed using SYBR™ Green Fast Advanced Cells-to-CT ™ Kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Membranes were washed three times for 5 min in PBST before being incubated with DAPI (50 nM; Sigma-Aldrich) as a nuclear counterstain ...
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bioRxiv - Microbiology 2020Quote: Time-lapse imaging was performed on 1.5% agarose pads embedded with 7H9 OADC medium containing ATc (Sigma, 100ng/ml) and kanamycin (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed with PBS 1X three times and cell nuclei were stained with 0.5μg/mL Hoechst (Sigma Aldrich) (diluted 1:5000) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times and secondary staining was done in permeabilization buffer supplemented with 5% Donkey serum (Sigma) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... the worms were washed three times with M9 and incubated in a 10 mM serotonin solution (Sigma Aldrich, 14927) for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were washed three times in PBS and blocked with 10% Normal Goat Serum in PBS with 0.1% Triton-X (Sigma) for 1 hour at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads were washed three times with NETN buffer and then eluted with 2 mg/ml biotin (Sigma-Aldrich) for 2 hr at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were further washed three times with PBS and incubated with 5% bovine serum albumin (BSA) (Sigma-Aldrich) in PBS for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were removed and the plate was washed two times with water before adding 0.1 % crystal violet (Sigma-Aldrich) for 10 min ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed with PBS-T 3 times before the plates were incubated with 75μl Streptavidin peroxidase (1:1000, Sigma) for 60 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... Slides were washed 3 times in PBS for 5 min and incubated in DAPI (Sigma Aldrich, Cat. No.10236276001) for nuclei counterstaining ...
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bioRxiv - Microbiology 2019Quote: ... The spheroplasts were then washed 3 times in SPM buffer permeabilized by incubation in 1 mL of 0.1% triton X-100 (Sigma) in PBS for 2 minutes on ice and washed twice in PBS ...
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bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
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bioRxiv - Immunology 2019Quote: ... The plates were washed three times with PBS-T and then blocked with 280 µl/well of assay buffer [2.5% (w/v) BSA (Sigma), 2.5% (v/v ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed four times and enzyme activity detected by incubation with 100 µl/well o-phenylenediamine (Sigma) containing hydrogen peroxide for 15 min at room temperature in the dark ...
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bioRxiv - Developmental Biology 2019Quote: ... Retention time (3.8 seconds) for 5-HT were determined by a control 5-HT dilution of 1pM (Sigma-Aldrich). Results are stated as area under the curve (AUC ...
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bioRxiv - Cell Biology 2019Quote: ... Gels were then washed three times with PBS and coated with either 10 µg/ml fibronectin (Merck-Millipore, 341631) (U2OS and U-251 cells ...
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bioRxiv - Microbiology 2021Quote: ... the conjugate underwent buffer exchange three times into 1× DPBS pH 7.4 using a 4-ml Amicon Ultra centrifugal filter (30 kDa MWCO, Millipore). The conjugate was characterized using SDS-PAGE ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... The samples were washed with PBS three more times and incubated with 1.0 mg/mL 4,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich) for nuclear staining ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were released via 0.12% trypsin without EDTA and washed 3 times with PBS (Sigma-Aldrich, St. Louis, MO). 0.4% trypan blue (Thermofisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: Cryo-sections were let to stabilize at room temperature for at least 2 hours and then washed three times in PBST (1X PBS with 0.1% Triton X-100, Sigma). To block endogenous peroxidase ...
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bioRxiv - Neuroscience 2021Quote: ... The PVDF membrane was rinsed three times with a 0.1 M Tris-buffered saline (TBS) solution containing 0.5% Tween-20 (TBS-Tween; Sigma-Aldrich) and blocked in TBS-Tween with 5% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... the culture medium was removed and cells were rinsed 3 times with Dulbecco’s Phosphate Buffered Saline (DPBS, Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... After 3 times washing with PBS-T [PBS with 0.5% (v/v) Triton X-100 (Sigma Aldrich, # X-100)] fixed brains were incubated with primary antibody containing 10% Normal Donkey Serum in 0.4% PBS-T overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were then rinsed 3 times with PBS followed by permeabilization with 0.1% triton X-100 (Sigma, X100) in PBS for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the conjugate underwent buffer exchange three times into 1x DPBS pH 7.4 using a 4-ml Amicon Ultra centrifugal filter (30 kDa MWCO, Millipore). The conjugate was characterized using SDS-PAGE ...