Labshake search
Citations for Millipore Sigma :
851 - 900 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... with a molecular weight cut-off (MWCO) 12–14 kDa and concentrated to 5 mg/ml using spin-filters (Centriprep, Millipore) with a MWCO of 10 kD ...
-
bioRxiv - Neuroscience 2023Quote: ... for 14–22 hours, followed by a stimulation with bicuculine methiodide (30 μM, WAKO) and 4-aminopyridine (100 μM, SIGMA) for 2 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... 50 ml of cells were grown in L1 media and collected 14 days after inoculum by filtration through Durapore 5 µm PDVF membranes (Millipore) using a manifold system ...
-
bioRxiv - Cell Biology 2023Quote: ... Myc3 fragment was obtained from ATF6α(C)-TAP2 (Myc3-TEV-ProteinA) (George et al., 2021) and inserted into A p3xFlag-CMV-14 expression vector (Sigma) at the site of BamHI to construct p3xMyc-CMV-14 expression vector ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were grown with or without Dox (0.5 µg/ml) for 8–14 d depending on the cell line and afterwards stained with crystal violet (Sigma-Aldrich). Colony number and area was determined on scanned plates using Fiji (Image J)57,58 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and histones were subjected to derivatization using 5 µL of propionic anhydride and 14 µL of ammonium hydroxide (Sigma-Aldrich) to balance the pH at 8.0 ...
-
bioRxiv - Biochemistry 2024Quote: ... were homogenized using a Polytron in 1 ml of 20 mM sodium acetate buffer containing 0.15 M NaCl and 1% Zwittergent 3-14 detergent (Millipore-Sigma 693017), pH 4.75 ...
-
bioRxiv - Molecular Biology 2021Quote: ... elution profiles and sample purity were confirmed by Western blot with antibodies against His tag (Sigma, H1029, lot 033m4785) and Strep tag (IBA ...
-
bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
-
bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
-
bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
-
bioRxiv - Neuroscience 2022Quote: ... A total of 3×106 co-transformants was initially screened for growth on synthetic defined media (SD)-Leu-/ Trp-/ Ura-/ His- media containing 20mM of 3-amino-1,2,4-triazole (3-AT, Sigma-Aldrich). Plasmids were isolated from the potential positive colonies ...
-
bioRxiv - Plant Biology 2021Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentration of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported (Putarjunan et al. ...
-
bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
-
bioRxiv - Molecular Biology 2020Quote: ... the His6-tag-fusion proteins were purified from the supernatant by a HIS-Select® Nickel Affinity Gel (Sigma) column ...
-
bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
-
bioRxiv - Neuroscience 2019Quote: ... Adult zebrafish were assayed for response to a mixture of 8 amino acids (Ala, Cys, His, Lys, Met, Phe, Trp, and Val; 0.1 mM each in distilled water; Sigma). No stimulation was provided for the first 5 mins following which the amino acid mix was introduced in one side of the tank at the rate of 1 ml/min using a syringe pump ...
-
bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
-
bioRxiv - Biochemistry 2021Quote: ... The resulting lysate was clarified by centrifugation and the supernatant was applied to Ni2+ -NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and His wash buffer (50 mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2021Quote: ... The his-tag affinity purified stator was concentrated to 1 ml using a 100 K MWCO Amicon device (Millipore). The samples were subjected to size exclusion chromatography using Enrich SEC650 column (Bio rad ...
-
bioRxiv - Microbiology 2020Quote: ... The culture media for the dividing cells (HI-10) consisted of RPMI-1640 medium + 10% heat inactivated FBS (Sigma), 100 μg/mL penicillin and 100 μg/mL streptomycin ...
-
bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
-
bioRxiv - Immunology 2021Quote: Expression cassette of maltose binding protein (MBP)-Mb2Cas12a-His in pMal vector was transformed to Rosetta 2 (DE3) (Novagen) competent cells ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
-
bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
-
bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
-
bioRxiv - Biochemistry 2024Quote: ... Supernatants were harvested and Fab fragment was purified via the heavy chain His tag using affinity chromatography with Talon resin as per the manufacturer’s specifications (Sigma). Purified protein samples were dialysed into PBS as quantified by absorbance at 280nm.
-
bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtered through a 0.22 µm filter then applied to a 5 mL His-TrapTM HP (Sigma) column pre-equilibrated with the same buffer at a flow rate of 1.5 mL.min-1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The 8 ml supernatant was applied to 1 ml packed volume of Ni-NTA His-Bind Resin (Millipore Sigma). Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad) ...
-
bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... The resulting lysate was clarified by centrifugation and the supernatant was applied to Ni2+-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and His wash buffer (50 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
-
bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein expression and native purification was performed by using His-select Nickel affinity gel resin column (Sigma Aldrich, USA) according to the manufacture’s instruction ...
-
bioRxiv - Biophysics 2023Quote: His-FUS LC was labeled with Atto-488 or Atto-647 NHS-ester fluorescent dyes (ATTO-TEC, Sigma-Alrich). Labeling occurred at or near the N-terminus because only the N-terminus and a lysine at residue position 5 in the leader sequence preceding the N-terminal region hexa-histidine tag were expected to react with the NHS-functionalized dye ...
-
bioRxiv - Microbiology 2023Quote: An N-terminal His-tagged fusion of HcpGA1 was constructed by cloning QR305_02952 into the NdeI–BamHI sites of pET16b (Novagen), and the plasmid was transformed into E ...
-
bioRxiv - Microbiology 2023Quote: ... The supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), incubated at room temperature for 20mins and poured into a 1-cm separation column (Bio-Rad) ...
-
bioRxiv - Plant Biology 2023Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentrations of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported 21 ...
-
bioRxiv - Plant Biology 2024Quote: ... benthamiana were tandem affinity-purified using a His-Bind resin followed by anti-Flag-M2-agarose beads (Sigma-Aldrich) as described32 with slight modifications ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.5 mg/mL mouse anti-Myc (EMD Millipore, 4A6 [mouse]), 1 mg/mL rabbit anti-Myc (EMD Millipore ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse anti mouse Nestin (Millipore, product no: MAB353, (1:100)) ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-γ tubulin and mouse anti-a tubulin (Sigma). Goat anti-mouse IRDye 680RD and goat anti-rabbit IRDye 800CW (LI-COR Biosciences ...
-
bioRxiv - Molecular Biology 2024Quote: ... and used for transduction of 1E+05 HEK 293T cells in a 24-well plate along with 8 μg/mL polybrene (Sigma Cat No. TR-1003-G) in 500 μL of complete DMEM ...
-
bioRxiv - Plant Biology 2021Quote: ... anti-mouse (Sigma), 1:10,000 ...
-
bioRxiv - Cancer Biology 2021Quote: ... mouse IgG (Sigma), and rat IgG (Sigma).
-
bioRxiv - Plant Biology 2020Quote: ... anti-mouse (Sigma) or anti-rabbit peroxidase-conjugated (Merck Millipore ...
-
bioRxiv - Neuroscience 2019Quote: ... Flag (Mouse, Sigma), vGLUT1 (Guinea Pig ...
-
bioRxiv - Microbiology 2019Quote: ... β-actin (mouse, Sigma), GAPDH (mouse ...
-
bioRxiv - Neuroscience 2019Quote: ... Flag(mouse, Sigma), GST(rabbit ...