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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Biochemistry 2024Quote: ... and lysates were clarified by ultracentrifugation (20,000 x g for 20 min) and incubated with 0.4 mL of His-Select nickel resin (Sigma) for 1 h at 4°C with gentle mixing ...
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... and the soluble fraction was purified by affinity chromatography (either Ni2+ or Strep) (His-Select resin (Sigma-Aldrich); Strep-Tactin®XT 4Flow resin (IBA Lifesciences)) ...
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bioRxiv - Biochemistry 2024Quote: Cleaved MntR was loaded onto a 5 ml column of Ni-NTA His•Bind® Resin (Millipore Sigma) equilibrated with Buffer A and then eluted with 40 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... or anti-mouse IgG (mouse, A9044, 1:10000,Sigma) at room temperature for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-STIM1 (Novus) or mouse anti-FLAG (Sigma) were used for IF staining ...
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bioRxiv - Cell Biology 2023Quote: ... Normal Mouse IgG (12-371, Sigma-aldrich, Mouse polyclonal).
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in OM with or without 10μM SP600125 for 7 or 14 days and tested by ARS staining (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Stage 14 egg chambers were treated with 10 μg/ml puromycin dihydrochloride from Streptomyces alboniger (Millipore Sigma, St. Louis, MO) in PBS on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... laevis were primed 2-14 days before ovulation by subcutaneous injection of 50 U pregnant mare serum gonadotropin (PMSG; Sigma) at the dorsal lymph sac ...
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bioRxiv - Microbiology 2019Quote: ... of Saksenaea dorisii strain BiMM-F232 (collected from CZA grown at 30°C for 14 days into physiological solution) was evenly distributed on RPMI-1640 (Sigma) medium plates ...
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bioRxiv - Genetics 2019Quote: ... and 14-mpf) with the following antibodies: primary—corneal keratan sulfate proteoglycan (CKS; 1:125; MAB2022; EMD Millipore, Billerica, MA), N-cadherin (cdh2 ...
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bioRxiv - Bioengineering 2020Quote: ... Nanoparticles were removed by spin filtering (14 krcf for 30 min; Amicon Ultra-0.5 mL centrifugal filters with 30 kDa MWCO, Millipore Sigma). Nanoparticle removal was done after protein digestion into peptides due to the otherwise very similar sizes of nanoparticles and proteins ...
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bioRxiv - Bioengineering 2020Quote: ... and spin-filtered to concentrate and remove impurities (14 krcf for 30 min; Amicon Ultra-0.5 mL centrifugal filters with 3 kDa MWCO, Millipore Sigma). Proteins were alkylated with 15 mM iodoacetamide for 30 min in the dark ...
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bioRxiv - Bioengineering 2020Quote: ... CSF was concentrated 10X prior to the incubation step to maintain the same protein to nanoparticle ratios under volume constraints (14 krcf for 30 min; Amicon Ultra-0.5 mL centrifugal filters with 3 kDa MWCO, Millipore Sigma). The ratio of protein concentration to nanoparticle surface area was maintained constant for each respective nanoparticle type in different biofluids ...
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bioRxiv - Microbiology 2020Quote: ... VLP samples were separated by 10% or 14% SDS-PAGE later blotted to PVDF membrane (Immobilon®-FL, Merck Millipore) and blocked overnight with ODYSSEY® blocking buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF7 cell lines were cultured during 14 to 21 days before Crystal violet coloration (0.01% final concentration; Sigma Aldrich #CO775) or MTT assay (1 mg/mL final concentration).
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bioRxiv - Plant Biology 2022Quote: ... total RNA was extracted from 14 day-old Arabidopsis thaliana seedlings using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) and treated with DNAase I (RapidOut DNA Removal Kit ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.5 g/L (days 0-5 and days 11-14) or 2.5 g/L (days 6-10) NaHCO3 (Sigma-Aldrich), and 0.25 mM ascorbic acid (days 3-10) ...
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bioRxiv - Bioengineering 2021Quote: ... total DNA content was isolated from the 3D constructs at 14 and 21 days using a DNA extraction kit (Sigma). The isolated DNA contents were collected and quantified by using Nano drop (Thermo Scientific-2000).
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bioRxiv - Pathology 2019Quote: Kidney sections were examined using immunofluorescence and confocal microscopy as described previously.14 Primary antibodies used were rabbit polyclonal APOL1 (1:400 dilution, Sigma), mouse monoclonal Synaptopodin (1:10 dilution ...
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bioRxiv - Biochemistry 2019Quote: ... STAT1 (0.3 µM) (Biosource, PHF0011) was phosphorylated in vitro by incubation with recombinant human cdk1/cyclinB (0.2 µM) (Millipore, 14-450) at 30 °C for 90 min in the presence of 1 mM ATP ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were digested for at least 14 h (overnight) with an animal-free recombinant bovine trypsin expressed in corn (Trypzean T3568 from Sigma) at 1/50 w/w and RT ...
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bioRxiv - Physiology 2019Quote: ... all mice were treated with doxycycline in the drinking water around 10-14 weeks of age (1g/L; Sigma Aldrich) for 3 weeks followed by a wash out period of 3 weeks.
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bioRxiv - Neuroscience 2021Quote: ... Fatty acids were converted to fatty acid methyl esters (FAME) by heating with 14% boron trifluoride (BF3) in methanol (Sigma) and separated on capillary columns in an HP 5890 Series II GLC equipped with a flame ionization detector ...
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bioRxiv - Physiology 2021Quote: ... 6 × 105 cells were seeded on 6-well multiwell plates and counted every two days from day 4 to day 14 using Trypan blue (Sigma) and a Neubauer chamber.
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bioRxiv - Microbiology 2021Quote: ... Rats in the designated groups were treated for 14 consecutive days with on average 1·5 mg/kg dopamine (H8502, Sigma), 0·0625 mg/kg pramipexole (A1237 ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with primary antibodies (anti-Vimentin, 1:200 dilution, Abcam; anti-cytokeratin 14, 1:200 dilution, Millipore, USA ...
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bioRxiv - Developmental Biology 2020Quote: ... Metaphase II-arrested oocytes tightly packed with cumulus cells were collected from the oviductal ampulla 14 h after hCG injection and placed in a 100-μl drop of human tubal fluid (HTF; Millipore) medium covered with mineral oil ...
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bioRxiv - Cell Biology 2021Quote: ... frogs were primed 2-14 days before ovulation by subcutaneous injection of 50 U pregnant mare serum gonadotropin (PMSG; Sigma) at the dorsal lymph sac ...
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bioRxiv - Immunology 2021Quote: ... HLA typed HLA-A*02:01+ PBMCs from COVID-19 recovered individuals were incubated with SARS-CoV-2 peptide pools (2µM / peptide) and cultured for 10-14 days in RPMI-1640 supplemented with 1x Non-essential amino acids (NEAA; Sigma), 5 mM HEPES (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: The phosphorylation of the S1/S2 linker constructs was examined in vitro using purified cyclin B-Cdk1 (Millipore 14-450) and PKA (murine cAMP-dependent protein kinase) ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were processed using the EpiCypher CUTANA™ CUT&RUN Kit (SKU: 14-1048) per the manufacturer’s instructions with 0.5 μg/100 μl anti-Hoxa11 (Sigma-Aldrich SAB1304728) or anti-IgG (CUTANA™ SKU ...
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bioRxiv - Developmental Biology 2022Quote: ... Fatty acids were then methylated with 2 mL of Boron Trifluoride (BF3) in Methanol (14% W/V) (Sigma Aldrich, USA) and incubated at 55 °C for 1.5 hours with intermittent shaking every 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were incubated in primary antibodies (Anti-LAMP1 1:100 Invitrogen CAT#14-1079-80; anti-Iba-1 1:250 Abcam CAT#ab5076; anti-dsDNA 1:250 Millipore CAT#MAB1293 ...
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bioRxiv - Cell Biology 2024Quote: ... Gel binding on the same glass coverslips as for PDMS gels was facilitated by activating the glass surface using a 1:1:14 solution of acetic acid (695092, Sigma)/bind-silane (10700467 ...
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bioRxiv - Cell Biology 2023Quote: ... 50 ml of cells were grown in L1 media and collected 14 days after inoculum by filtration through Durapore 5 µm PDVF membranes (Millipore) using a manifold system ...
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bioRxiv - Cell Biology 2023Quote: ... Myc3 fragment was obtained from ATF6α(C)-TAP2 (Myc3-TEV-ProteinA) (George et al., 2021) and inserted into A p3xFlag-CMV-14 expression vector (Sigma) at the site of BamHI to construct p3xMyc-CMV-14 expression vector ...
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bioRxiv - Neuroscience 2022Quote: ... both followed by 150 mL Lana’s fixative (room temperature, 4% depolymerized paraformaldehyde and 14% saturated picric acid solution, Sigma Aldrich, P6744-1GA ...
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bioRxiv - Microbiology 2023Quote: ... were fed through a mesh-covered container on mice anesthetized with 4-6 μL/g ip of 14 mg/mL ketamine (67457010810, Myland Pharmaceuticals) plus 1.2 mg/mL xylazine hydrochloride (X1251-1G, Sigma Aldrich) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Fbp1F/F and Fbp1ΔHep mice that were HFD fed for 12 wk or NCD-8 wo mice were fasted for 12-14 h and then given 1 g/kg glucose or sodium pyruvate (2 g/kg, Sigma) by i.p ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting cell-modified graphite electrodes were encased in a dialysis membrane (14 kDa molecular weight pore size, Sigma-Aldrich) that was previously soaked in deionized water for 1 h before insertion in the electrochemical cell ...
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bioRxiv - Cell Biology 2023Quote: ... Inserted products were excised using the Hind III restriction site and ligated into pre-digested p3xFLAG-CMV-14 (Sigma Aldrich) cut with the same enzyme.
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bioRxiv - Cell Biology 2023Quote: ... cells were grown in complete medium and for days 8-14 this medium was supplemented with 50μM L-ascorbic acid (Sigma-Aldrich). Medium changes were performed every 3 days ...
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bioRxiv - Genomics 2023Quote: ... and histones were subjected to derivatization using 5 µl of propionic anhydride and 14 µl of ammonium hydroxide (Sigma Aldrich) to balance the pH at 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... 14 hours post-infection the cells were washed 2x with Hanks□ Balanced Salt solution (HBSS, Sigma-Aldrich Cat. Nr. H8264) and fixed with 4 % PFA in PBS (Ampliqon Cat ...
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bioRxiv - Biochemistry 2023Quote: ... Twenty μl of each sample were diluted 14-fold in HDX-MS buffer made with 99.9% D2O (151882-100G, Sigma-Aldrich) to mimic the exposure conditions for HDX-MS experiments ...