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Citations for Millipore Sigma :
851 - 900 of 10000+ citations for 8 Chloro 2 3 4 5 tetrahydropyrido 3 2 f 1 4 oxazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Immediately prior to plating on agar plates containing 20 mM 4-chloro-phenylalanine (Sigma-Aldrich), the cells were washed twice with phosphate-buffered saline (PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... 4-Chloro-DL-phenylalanine and agarose were added as appropriate: erythromycin (Sigma, 25 μg/mL), gentamicin sulfate (TEKnova ...
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bioRxiv - Cell Biology 2020Quote: ... and resuspended in HBSS with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/ml, Sigma) for flow cytometry ...
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bioRxiv - Microbiology 2020Quote: ... Nucleic acids were stained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma, 1 µg/mL) and the cells were washed three times with PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunofluorescent sections were counterstained with 4′,6′-diamidino-2-phenylindole (DAPI, 1:3000; Sigma-Aldrich) and mounted with FluoroGel (Electron Microscopy Services) ...
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bioRxiv - Neuroscience 2020Quote: ... All sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 1:1000). Secondary antibodies from Jackson Laboratories were used at 1:400.
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bioRxiv - Neuroscience 2021Quote: ... The nuclei were stained with DAPI (1:1000, 4’, 6-diamidino-2-phenylindole dihydrochloride; Sigma). The absence of labeling when using sense probes was used to confirm the specificity of the anti-sense probes ...
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bioRxiv - Systems Biology 2021Quote: ... diluted 1:200 in blocking reagent) and 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) was used at 200 ng/mL for nuclei staining ...
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bioRxiv - Bioengineering 2022Quote: ... at 4°C for 2 h and blocked with 1% bovine serum albumin (Sigma-Aldrich), 3% donkey serum (Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were stained using 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich #D8417, 1:1000).
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were stained with DAPI (4′, 6-diamidino-2-phenylindole, 1 μg/mL, Sigma-Aldrich). Images were acquired on a point-scanning confocal microscope SP8 Leica with an oil 40x objective 1.25 N.A ...
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bioRxiv - Biophysics 2023Quote: ... Nucleus were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 1:1000; Sigma-Aldrich) for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, 1 µg/ml, Sigma, USA) for 10 min in the dark.
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bioRxiv - Developmental Biology 2023Quote: ... The sections were then stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:3000, Sigma) diluted in TBS for 1 min and then finally rinsed three more times with TBS ...
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bioRxiv - Neuroscience 2020Quote: ... SYSY), vesicular glutamate transporter 2 (VGLUT2; 1:1,000, SYSY) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:50,000, Millipore) at 4 °C for 16 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Oligonucleotides 5’-GAAAAAAAAAATATACGCTAAGATTTTTGG-3’ and 5’-ATGACTAAACCCCCCCTCC-3’ synthesized and HPLC-purified by Sigma-Aldrich were used ...
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bioRxiv - Physiology 2023Quote: ... CDH1 Forward 5′-TTACTGCCCCCAGAGGATGA-3′ and Reverse 5′- TGCAACGTCGTTACGAGTCA-3′;) were purchased from Sigma-Aldrich. mRNA expression was determined using the comparative 2-ΔΔCt method and normalized to the mRNA expression level of endogenous reference (PPIA ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of cell culture medium were incubated at 37°C for 3 hours with 20 µL reaction mix containing sodium citrate (10 mM; pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...
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bioRxiv - Immunology 2023Quote: ... Media was removed from the apical compartment of the transwell and replaced by 200µL of fresh medium containing of 2 mg/mL fluorescein isothiocyanate (FITC)-labelled dextran (4 kDa) (Sigma-Aldrich, # 46944-500MG-F). As a negative control and to calculate the background ...
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bioRxiv - Neuroscience 2022Quote: ... data was separated into pre-defined frequency bins (Delta 0.5-4 Hz, Theta 4-8 Hz, Alpha 8-13 Hz, Sigma 12-15 Hz ...
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bioRxiv - Biochemistry 2019Quote: ... Cell pellets were resuspended in PBS and mixed with the equal volume of lysis buffer (3 M NaCl, 100 mM Tris-HCl pH 7.5, 4 mM EDTA pH 8, 20 mM ß-mercaptoethanol, Sigma Protease Inhibitor Cocktail (Sigma)) ...
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bioRxiv - Microbiology 2020Quote: ... 4 μL of Cell Counting Kit-8 (CCK-8; Sigma-Aldrich) was added to each well ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM 4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride (AEBSF) and 1 complete ULTRA protease inhibitor tablet (Sigma-Aldrich)) then clarified by centrifugation at 14,000 x g for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... carbonyl cyanide 5-chloro-phenylhydrazone (CCCP) (Sigma), PLD2 inhibitor VU 0364739 hydrochloride (Tocris 4171) ...
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bioRxiv - Neuroscience 2019Quote: ... and spinal cords of both sexes (n = 3 each) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Developmental Biology 2020Quote: ... Fertilized oocytes were collected from 3-4 week-old superovulated C57Bl6 females prepared by injecting 5 IU each of pregnant mare serum gonadotrophin (PMSG) (Sigma Aldrich) and human chorionic gonadotropin (hCG ...
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bioRxiv - Plant Biology 2021Quote: ... sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418, SIGMA)) ...
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bioRxiv - Bioengineering 2022Quote: ... Bone-marrow derived hMSCs (hBMSCs) were frozen at passage 3 or 4 with 5*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Cell Biology 2019Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Sigma-Aldrich) for 5-30 min at RT ...
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bioRxiv - Biochemistry 2019Quote: ... anti-tubulin (B-5-1-2, Sigma), anti-FLAG (M2 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µM 5-fluoro-2’-deoxyuridine (Sigma), penicillin ...
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bioRxiv - Cell Biology 2021Quote: ... alpha-tubulin (B-5-1-2, Sigma), tyrosinated tubulin (YL1/2 ...
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bioRxiv - Cell Biology 2019Quote: ... After washing 3–4 X with 1× PBS-T the strips were incubated with 1:5000 mouse anti-GST antibodies (Sigma). The secondary antibodies (anti-rabbit-HRP or anti-mouse-HRP ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated 1-3 overnights at 4°C in the following primary antibodies: goat anti-choline acetyltransferase (1:200; Millipore), rabbit anti-substance P (1:500 ...
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bioRxiv - Immunology 2023Quote: ... Th17 cells were split (usually 1:3) and transduced by adding 1:80-diluted retroviral supernatant and 4 µg/ml polybrene (Sigma) in addition to 10 ng/ml IL-23 and 10 µg/ml anti-IFNγ antibody (all diluted in pre-warmed T cell medium and calculated for culture volume) ...
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bioRxiv - Genetics 2019Quote: ... 1000 μg whole cell lysates were incubated with the indicated anti-Flag M2 affinity gel and monoclonal anti-HA agarose for 3-4 hr at 4 degree (Millipore Sigma). Immunoprecipitants were washed five times with NETN buffer (20 mMTris ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: For multiphoton microscopy kidneys were fixed in 4% paraformaldehyde overnight at 4 °C and embedded in 3% low-melting point agarose (Sigma- Aldrich). Sections (500 µM ...
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bioRxiv - Immunology 2022Quote: ... 10 µl of hemolymph was mixed with 90 µl of 3, 4-Dihydroxy-L-phenylalanine (L-DOPA, 4 mg/ml)(Sigma Aldrich) dissolved in nuclease free water (Cytiva) ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 3-4 h at 4 °C with 15-20 μL of ANTI-FLAG® M2 Affinity Gel (Sigma) and washed 3-4 times with Co-IP wash buffer containing 0.1% Triton-100 but without Phosphatase Inhibitor Cocktails and Protease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2023Quote: Organoids were seeded in 15-well Ibidi chambers after passaging and fixed 3-4 days later in 4% Paraformaldehyde (Sigma-Aldrich) for 60 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 2-4 months old mice were orally gavaged with 3 x 2 (Mist1creERT/+KRASG12D/+) or 5 x 5 mg (Ptf1acreERT/+KRASG12D/+) of tamoxifen (TX; Sigma-Aldrich, T5648-5G) at Western University and 5 x 4 mg (Ela-creERT ...
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bioRxiv - Biophysics 2021Quote: NmeCas9 was expressed in Rosetta 2(DE3) cells (Millipore Sigma, 71400-3) and grown in LB supplemented with 100 µg/mL Carbenicillin and 34µg/mL Chloramphenicol ...
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bioRxiv - Immunology 2019Quote: ... the MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) powder is re-suspended into PBS ...
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bioRxiv - Neuroscience 2020Quote: ... containing Phosphatase Inhibitor Cocktails 2 and 3 (Sigma-Aldrich, St. Louis, MO) and MiniComplete Protease Inhibitor Cocktail (Roche Diagnostics) ...
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bioRxiv - Cancer Biology 2021Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) (Sigma-Aldrich) for the MTT assay was dissolved in PBS in stock concentration 5 mg/mL and stored at 4 °C in the dark ...
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bioRxiv - Neuroscience 2022Quote: 2’-3’-dideoxycitidine (ddC) was purchased from Sigma Aldrich (St. Louis, MO). AM1710 was synthesized by the lab of Alexandros Makriyannis (Northeastern University ...
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bioRxiv - Cancer Biology 2022Quote: ... containing phosphatase inhibitor cocktails 2 and 3 and protease inhibitors (Sigma-Aldrich), for 30–45min at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... 400μL (3 mg/mL) of methylbenzothiazolinone-2-hydrazone (MBTH, Sigma M8006−1G), 200μL of sample and 0.5μI of alcohol oxidase (E.C ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 µl (3–4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma) were added to each well and the plate incubated at 37°C for 3 h ...