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Citations for Millipore Sigma :
851 - 900 of 10000+ citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The instrument was calibrated against a poly(ethylene glycol) methyl ether standard (Sigma Aldrich, Average Mn = 2,000 g mol-1) prepared under the same conditions with DCTB matrix.
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bioRxiv - Cell Biology 2024Quote: ... Fully grown oocytes were collected 48 hours after injection and placed in M2 medium containing 200 μM 3-isobutyl-1-methyl-xanthine (IBMX, Sigma) at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and contained 800 μl culture media (Leibovitz’s L15 Medium, no phenol red, Thermo Fisher 21083027; 50 ng/μl Protein A; 0.01% Methyl Cellulose, Sigma 274429). Surface conditions were critical to successful cultures ...
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bioRxiv - Neuroscience 2024Quote: ... the brains were cryoprotected with 30% sucrose in phosphate buffer saline (PBS; 0.1 M; pH 7.4) and frozen with isopentane (2-methyl butane, Sigma-Aldrich, Germany) at −60/−70°C following the protocol described by Rosene et al ...
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bioRxiv - Cell Biology 2020Quote: ... then a 6-fold molar excess of Maleimide-PEG8-succinimidyl ester (Sigma 746207) was added ...
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bioRxiv - Biochemistry 2022Quote: ... Pellets were resuspended in lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM MgSO4, 5 mM 6-aminocaproic acid (Sigma, cat. #A2504), 5 mM benzamidine (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... FISH probes were labelled with 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester (FLUOS) or with the sulfoindocyanine dyes (Cy3) at the 5’ end of the probes (Sigma Aldrich company, Germany). FISH sample preparation was conducted as described earlier (Fernando et al. ...
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bioRxiv - Physiology 2023Quote: ... Cells were incubated with P-gp substrate calcein-acetoxymethyl ester (Ca-AM, 177831, 10−6 M, Sigma; 37°C, 5% CO2, 1 hour). Once substrate was added ...
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bioRxiv - Cell Biology 2020Quote: ... Linoleic acid (C18:2n-6, Sigma # L1376), γ-linolenic acid (C18:3n-6 ...
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bioRxiv - Cell Biology 2020Quote: ... γ-linolenic acid (C18:3n-6, Sigma # L2378), Dihomo-γ-linolenic acid (C20:3n-6 ...
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bioRxiv - Biophysics 2022Quote: ... 6 μL acrylic acid (147230, Sigma Aldrich), 84 μL 1M NaOH and 10 μL 500nm-diameter yellow-green fluorospheres was prepared (F8813 ...
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bioRxiv - Cell Biology 2023Quote: ... docosahexaenoic acid (DHA 22:6; Sigma-Aldrich) and oleic acid (OA 18:1 ...
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bioRxiv - Cell Biology 2022Quote: ... and 5-PVA (5-phenylvaleric acid, Sigma) were dissolved in equimolar amount of NaOH to prepare a 0.5M stock solution with pH 7.
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bioRxiv - Developmental Biology 2022Quote: ... and (+−)-6-hydroxy-2,5,7,8- tetra- methylchromane-2-carboxylic acid (Trolox) (cat: 238813-5 G) were ordered from Sigma. Potassium chloride (cat ...
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bioRxiv - Cell Biology 2023Quote: ... and (+−)-6-hydroxy-2,5,7,8-tetra-methylchromane-2-carboxylic acid (Trolox) (cat: 238813-5 G) were ordered from Sigma. Sodium hydroxide (cat ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... and (+−)-6-hydroxy-2,5,7,8-tetra-methylchromane-2-carboxylic acid (Trolox) (cat: 238813-5 G) were ordered from Sigma. 1× Phosphate Buffered Saline (PBS ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.1% w/v Ferrozine (Disodium-4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzosulfonate (Sigma-Aldrich) in dd ...
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bioRxiv - Cell Biology 2020Quote: ... Fatty acid methyl esters (FAMEs) were generated by dissolving dried fatty acids in 0.5 mL 2% (v/v) methanolic sulfuric acid (Sigma-Aldrich) and incubating at 50°C for 2 hours ...
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bioRxiv - Biophysics 2022Quote: ... anaesthetized with 0.02% aminobenzoic acid ethyl ester (tricaine, Sigma-Aldrich St. Louis, MO, USA), and dechorionated using tweezers ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.1% v/v acrylic acid N-hydroxysuccinimide-acrylamide ester (AA-NHS, Sigma-Aldrich) to facilitate the binding of fluorophores and proteins ...
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bioRxiv - Microbiology 2024Quote: ... 500 μM of methoxy-polyethylene glycol maleimide with an average molecular weight of 5 kDa (PEG5000-mal, Sigma) was added to the C ...
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bioRxiv - Microbiology 2020Quote: ... cholera toxin B subunit (CtxB)-FITC conjugate (C1655), methyl-β-cyclodextrin (Mβ-CD, C4555) and chlorpromazine hydrochloride (CPZ, C8138) were procured from Sigma.
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bioRxiv - Cancer Biology 2020Quote: ... Each spheroid was made by resuspending 8,000 cells in 15 μl media with 0.24% methyl cellulose (4,000 cpi, Sigma-Aldrich, St. Louis, MO) by the hanging drop method 34 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were fluorescently labeled using methyl green (Prieto et al, 2014) and actin filaments with TRITC-conjugated phalloidin (Sigma-Aldrich, P1951). Observation of whole embryos was performed using a Zeiss LSM 880 or a Zeiss LSM 800 laser confocal microscope ...
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bioRxiv - Developmental Biology 2020Quote: ... Gentle pressure was applied to epididymis tissue using forceps and sperm were allowed to swim out during a 45-minute incubation in HTF + 0.75mM methyl-β-cyclodextrin (Millipore MR-070-D, Sigma C4555) at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... LMPs were cultured in this cholesterol-free medium for 20 t0 24 hrs and then treated with 10mM methyl-β-cyclodextrin (MβCD, Sigma C4555) for 15min at 37°C to remove cholesterol from plasma membranes ...
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bioRxiv - Systems Biology 2020Quote: ... TBDMS (N-methyl-N-(t-butyldimethylsilyl) trifluoroacetamide + 1% t-butyl-dimethylchlorosilane) and MSTFA (N-methyl-N-(trimethylsilyl) trifluroacetamide) were also purchased from Sigma-Aldrich. Media components and other reagents were purchased from Himedia and Sigma-Aldrich.
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bioRxiv - Cell Biology 2021Quote: ... 100 nM of the specified SPR2-GFP proteins along with 2.5 μM 1:9 rhodamine-labeled porcine tubulin were then introduced into the flow chamber in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrin), 50 mM DTT and an oxygen-scavenging system consisting of 250 μg/ml glucose oxidase ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule polymerization was initiated by flowing in 20 mM 1:25 rhodamine-labeled porcine tubulin in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrich), 50 mM DTT ...
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bioRxiv - Physiology 2021Quote: ... female C57BL/6J mice (age 10-11 weeks) were given 2mg/ml IDO1 inhibitor (1-methyl-L-tryptopham, L1MT; Sigma-Aldrich) in drinking water supplemented with Splenda sweetener (2 sachets/liter ...
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bioRxiv - Plant Biology 2021Quote: ... tomato leaflets from the 3rd leaf were detached and immediately dipped for 10 sec in a 50 μM or 100 μM methyl-jasmonate (MeJA; Sigma-Aldrich) or mock solution (0.1 % EtOH ...
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bioRxiv - Bioengineering 2021Quote: ... with the control and labeling diets containing regular L-methionine (Met) and methyl-13C-L-methionine (13C-Met, 99%, Sigma-Aldrich), respectively (i.e. ...
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bioRxiv - Plant Biology 2021Quote: ... were permethylated by two rounds of treatment with sodium hydroxide (15 minutes each) and methyl iodide (45 minutes each) in DMSO (Sigma, USA) (46) ...
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bioRxiv - Physiology 2022Quote: ... 70 animals were placed onto each 50 mm tight-lidded petri plate with modified NGM containing 40 mM paraquat (or methyl viologen dichloride, from Sigma-Aldrich), 51 μM FUdR ...
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bioRxiv - Physiology 2022Quote: ... young adult animals were transferred to 1 mM auxin plates supplemented with 20 mM paraquat (Methyl viologen dichloride hydrate, Sigma-Aldrich). Animals were scored as in lifespan assays but twice a day ...
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bioRxiv - Neuroscience 2020Quote: ... the mice were anesthetized with 20 mg/kg 1.25% Avertin (a mixture of 12.5 mg/mL of 2,2,2-Tribromoethanol and 25 μL/mL 2-Methyl-2-butanol, Sigma, St. Louis, MO, USA) through intraperitoneal injection and then placed in a prone position in the stereotaxic frame ...
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bioRxiv - Physiology 2021Quote: ... animals were transferred at a density of 70 worms per plate to 50 mm tight-lidded plates with modified NGM containing 40 mM paraquat (or methyl viologen dichloride, from Sigma-Aldrich), 51 μM FUdR ...
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bioRxiv - Immunology 2020Quote: ... cells were treated for 1 h with a range of concentrations of the DNA methylating agent methyl methanesulfonate (MMS) (Sigma-Aldrich), and subsequently cultured for an additional 48 h ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were collected at various timepoints and the reaction was terminated by addition of SDS-PAGE sample buffer containing 100 mM methyl methanethiosulfonate (MMTS) (Sigma Aldrich). The PEGylation reaction samples were analysed using non-reducing polyacrylamide gels ...
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bioRxiv - Cell Biology 2020Quote: ... solutions of PVDF-TrFE (70/30) (400 kDa., PDI of 2.1, Solvay Solexis, Inc., Thorofare, NJ) in methyl ethyl ketone (MEK; Sigma-Aldrich, Inc.) was loaded into a syringe with an 18-gauge needle ...
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bioRxiv - Cancer Biology 2022Quote: ... mice were randomized and treated with 25 mg/kg Crizotinib or the corresponding dose of vehicle (hydroxypropyl methyl cellulose, Sigma Aldrich) (oral gavage ...
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bioRxiv - Neuroscience 2022Quote: ... was carbon filtered and passed through PFA vials (Savillex 200-30-12) containing odor (2% methyl valerate in mineral oil, Sigma Aldrich product #1489977 ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were derivatized for GC-MS analysis by adding 10 μL of pyridine and 50 μL of N-Methyl-N-(trimethylsilyl)trifluoroacetamide (Sigma-Aldrich) to the residue ...
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bioRxiv - Microbiology 2024Quote: ... In-cell SHAPE modifications were made by adding fresh 500µl of 100mM 1-Methyl-7-nitroisatoic anhydride (1M7) (Sigma-Aldrich, 908401) in DMSO to 4.5ml pre-warmed culture media to the dish and incubated for 3min at 37C ...
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bioRxiv - Immunology 2024Quote: ... The small intestine sections were rolled up from distal-to-proximal into “swiss rolls.” The swiss rolls were embedded in Optimal Cutting Temperature (OCT) (Sakura Finetek) and flash frozen in 2-methyl butane (Sigma Aldrich) over dry ice ...
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bioRxiv - Bioengineering 2024Quote: ... as inhibitor), and poly(ethylene glycol) methyl ether methacrylate (MEMA-PEG, 950 g/mol, MEHQ as inhibitor) are purchased from Sigma Aldrich and purified by recrystallizing in acetone to remove inhibitors ...
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bioRxiv - Systems Biology 2023Quote: ... followed by an incubation at 40°C for 1 h and then adding 70 μl of MSTFA (N methyl-N-trimethylsilyltrifluoroacetamide; Sigma-Aldrich) followed by another incubation at 40°C for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... and the remaining un-labelled EdU sites were then saturated by click-it chemistry with an excess of non-fluorescent azide (Azido-methyl-phenyl-sulfide 95%, Sigma, #244546): the reaction was prepared and achieved according to the manufacturer’s recommendations except the standard fluorescent azide was replaced by the non-fluorescent one at a final concentration of 2mM ...
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bioRxiv - Microbiology 2023Quote: The enzymatic production of DsrC-trisulfide was performed inside a Coy anaerobic chamber (98% N2, 2% H2) at 60 °C using Zn-reduced methyl viologen (Sigma-Aldrich) as the electron donor (7) ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ul of reaction was passed over a Dowex cation exchange resin column (N-methyl D-glucamine form) (217484; Millipore-Sigma) to remove any uninternalized 14C-lysine ...