Labshake search
Citations for Millipore Sigma :
801 - 850 of 10000+ citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... animals received daily intraperitoneal (I.P) injections of L3,4-dihydroxyphenylalanine methyl (L-DOPA, 3 day at 3mg/kg, then 6mg/kg, Sigma-Aldrich) and the peripheral DOPA decarboxylase inhibitor bensezaride hydrochloride (12mg/kg ...
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bioRxiv - Neuroscience 2022Quote: Lesion animals (n=18) received bilateral excitotoxic HPCv lesions via intracranial injections of N-methyl-d-aspartate (NMDA, Sigma, St-Louis ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 M NaCl) before eluting bound proteins with buffer A containing 0.5 M Methyl-α-D mannose (Sigma). Fractions containing NA activity were pooled ...
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bioRxiv - Physiology 2024Quote: Mice subjected to SNT were anesthetized through intraperitoneal injection of 0.5 mg/g avertin (2,2,2,-Tribromoethanol 97%, Sigma #T48402, and 2-methyl-2-butanol 99%, Sigma #240486-100ml). Soleus muscles were freshly dissected and embedded into O.C.T compound (23-730-571 ...
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bioRxiv - Cancer Biology 2024Quote: ... pre-diluted in 80/20 methanol:water and 50µL 0.02 ng/µL Propionyl-L-carnitine-(N-methyl-d3) (Sigma Aldrich 52941). After vortexing for 1 min samples were retuned to −80°C for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... For PILO-SE induction,8,16,17 mice were injected subcutaneously (s.c.) with the muscarinic antagonist scopolamine methyl nitrate (1 mg/kg, s.c.; Sigma Aldrich) to reduce the peripheral effects of PILO ...
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bioRxiv - Microbiology 2023Quote: ... and 200 μL aliquots were cultured in the presence or absence of paraquat (Methyl viologen dichloride hydrate, Sigma-Aldrich) in 96-well microplates at 37 °C for 24 h with shaking ...
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bioRxiv - Biochemistry 2022Quote: ... followed by another incubation with 20 µL N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide with 1% tert-Butyldimethylchlorosilane (TBDMS) (Sigma) at 60 °C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: Luria-Bertani medium and Bushnell Hass medium were used for primary [17] and secondary screening [18] of dye degradation capability of Serratia marcescens. Congo red (CR) (C.I. 22120) and Methyl orange (MO) (C.I. 13025) were purchased from Sigma Aldrich. All reagents used were of analytical grade ...
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bioRxiv - Plant Biology 2023Quote: ... (2) addition of 15 μL of derivatization grade N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA; Sigma-Aldrich; Catalog Number 394866) and incubation at 37°C for 30 min with agitation at 750 RPM ...
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bioRxiv - Neuroscience 2023Quote: ... methyl-tert butyl ether and ammonium acetate (LC-MS grade) were purchased from Sigma Aldrich (St. Louis, MO, USA), internal standard mixture SPLASH® LIPIDOMIX® was obtained from Avanti Polar Lipids (Alabaster ...
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bioRxiv - Cancer Biology 2024Quote: ... labelled cells were suspended in 2mL of medium containing 20μg/mL of soluble collagen I (BioEngineering) and 4.8mg/mL of Methyl Cellulose (MTC, Sigma-Aldrich). 20μL drops of the cell suspension were then hanged on the lid of a 10cm tissue culture dish to generate the spheroids ...
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bioRxiv - Cell Biology 2024Quote: ... 3) paraquat: worms were placed on standard NGM/OP50 plates treated with 25 mM paraquat (methyl viologen; Sigma Aldrich) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: Brain samples were embedded in 4-5% agarose (Sigma-Aldrich: 9012-36-6) in 0.1M PB and imaged using serial two-photon tomography (Han et al 2018 ...
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bioRxiv - Genetics 2022Quote: ... 5’-fluoroorotic acid (5’FOA; Sigma) was used at a concentration of 1 g/L in PMG containing 45 mg/L uracil ...
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bioRxiv - Molecular Biology 2024Quote: ... 5-Fluoroorotic acid (5-FOA; Sigma), Blasticidin (Invivogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% DMSO and 300 μM 3-maleimidobenzoic acid N-hydroxysuccinimide ester (MBS; Sigma) dissolved in microtubule stabilization buffer specific for algae (MTB ...
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bioRxiv - Bioengineering 2023Quote: ... the larvae were anesthetized with tricaine (3-aminobenzoic acid ethyl ester, Sigma Aldrich) and immobilized in 1% low-melting-point agarose inside a fluorinated ethylene propylene tube for further imaging.
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bioRxiv - Evolutionary Biology 2020Quote: ... Both fins of each genotyped larva were dissected and transferred to a drop of Methyl Cellulose 3% (M0387, Sigma-Aldrich) on a slide for documentation using an Olympus SZX16 (Model SZX2-ILLB ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were allowed to grow for 96 h in drug containing medium (methyl methanesulfonate, Acros Organics 156890250, mitomycin C, Sigma M4287 ...
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bioRxiv - Microbiology 2021Quote: ... the samples were dissolved in 50 μL of 20 mg/ml methoxyamine hydrochloride in pyridine for 1.5 h at 33°C followed by derivatization with N-Methyl-N-(trymethylsolyl)trifluoroacetamide (MSTFA, Sigma Aldrich) for 2 h at 35°C.
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bioRxiv - Biophysics 2021Quote: D-glucose and 3-O-methyl-D-glucose powder for in vitro studies were obtained from Sigma-Aldrich (Milan, Italy). Solutions of D-glucose and 3OMG for in vitro studies were prepared in 10 mM phosphate-buffered saline (PBS 1X) ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... and incubated at 37° C for 90 minutes followed by addition of 20μL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert–Butyldimethylchlorosilane (Sigma 375934) and incubated at 60°C for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated at 37°C for 90 minutes followed by addition of 20µL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert– Butyldimethylchlorosilane (Sigma 375934) and incubated at 60°C for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Cysteine Biotinylation was carried out using Poly(ethylene glycol) [N-(2-maleimidoethyl)carbamoyl]methyl ether 2-(biotinylamino)ethane (Sigma 757748) (Biotin-maleamide) ...
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bioRxiv - Biochemistry 2022Quote: ... Blocking Buffer (100 mM HEPES, pH 7.5 [Gibco], 1 mM EDTA [Sigma-Aldrich], 2.5% SDS [Sigma-Aldrich], 0.1% S-methyl methanethiosulfonate [Sigma-Aldrich]), Binding Buffer (100 mM HEPES ...
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Cell Biology 2022Quote: A solution of methimazole (1-methyl-3H-imidazole-2-thione) (CAS 60-56-0; MW, 114.17 g/mol; purity, ≥99%; Sigma-Aldrich) was used to induce hypothyroidism ...
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bioRxiv - Plant Biology 2021Quote: ... The sample was then derivatized by adding 60 μL N-methyl-N-(trimethylsilyl)trifluoro acetamide (MSTFA) (Cat# 69479; Sigma-Aldrich) and incubating for 30 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... ALP activity was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Biochemistry 2020Quote: ... These were mounted in a drop of in 0.1 M phosphate buffer containing 1:1 of 2% methyl cellulose (Sigma-Aldrich) and 2.3 M sucrose on TEM grids with a carbon-coated Formvar film (Taab Laboratory Equipment Ltd) ...
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bioRxiv - Neuroscience 2021Quote: The following odors/concentrations were used for comparing molecular properties to receptor responses: 1% 2-methyl-2-pentenal (Sigma 294667), 1% trans-cinnamaldehyde (Sigma C80687) ...
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bioRxiv - Developmental Biology 2021Quote: ... 70nm Sections were picked up using a 1 :1 mixture of 2.3M sucrose and 2% methyl cellulose (cat# M-6385, Sigma-Aldrich) as a pick-up solution and sections were transferred to carbon and formvar coated mesh grids (cat# H100-Cu ...
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bioRxiv - Cell Biology 2021Quote: ... sphere (1.5 μm – 3 μm)) coated with 8.45 mM poly(ethylene glycol)methyl ether thiol (Mn 800, Cat. No. 729108, Sigma Aldrich) solution for 2 h at room temperature were used ...
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bioRxiv - Cell Biology 2022Quote: ... Inhibitors and drugs (etoposide, trimethoprim, hydroxyurea, methyl methanesulfonate MMS, HIV protease inhibitors, E64, chloroquine and artemisinin) were from Sigma-Aldrich, Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Neuroscience 2022Quote: ... Compounds used in these studies are as follows: N-methyl-D-aspartate (NMDA; 1.5ul of 100mM in PBS; Sigma; M3262), recombinant mouse IL1β (200 ng/dose ...
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bioRxiv - Bioengineering 2022Quote: ... 80 μL of the supernatant was mixed with 20 μL of 0.75M 2-amino-2-methyl-1-propanol buffer (A65182, Sigma-Aldrich) and 100 μL 100 mM p-nitrophenylphosphate solution (71768 ...
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bioRxiv - Biophysics 2024Quote: ... methyl viologen (MV) (catalog no. 856177-1G) and sodium hydroxide (catalog no. 30620-1KG-M) were obtained from Sigma-Aldrich. Methanol (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... and 2-(14-carboxytetradecyl)-2-ethyl-4,4-di methyl-3-oxazolidinyloxy (16-doxyl-stearicacid, 16-DSA, cat. no. 253596) from Sigma-Aldrich. 5-DSA (spin probe containing the nitroxide group attached on C5 that is located on the opposite terminal relative to the charged carboxyl fatty acid terminus ...
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bioRxiv - Neuroscience 2024Quote: ... the bound protein was visualized using 100 µl of Tetra-methyl-benzidine liquid substrate (TMB slow kinetic form, Sigma-Aldrich) at RT for 20 min ...
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bioRxiv - Plant Biology 2024Quote: ... The tissue was blotted on tissue paper before weighing to remove excess water and then was transferred into a 1.5mL Eppendorf tube containing 1 mL of di-methyl sulfoxide (DMSO) (#D8418, Sigma Aldrich) and incubated in the dark at 65C with 300 rpm shaking for 45 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... TRACER scaffolds were manufactured by infiltrating cellulose paper strips (0.5 x 12 cm, Miniminit Products) with poly(methyl methacrylate) (PMMA, 120 kDa) dissolved in acetone (Sigma-Aldrich) at 0.175 g/mL with an Allevi Beta 1 3D printer ...
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bioRxiv - Immunology 2023Quote: ... HPLC grade methyl tert-butyl ether (MTBE), chloroform, ammonium fluoride (NH4F) (ACS reagent, ≥ 98%) and ammonium formate from Sigma-Aldrich or VWR ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: Visualization of the accessible pool of cholesterol was performed after 30 min treatment with 10 mM methyl-beta-cyclodextrin (MβCB) in 1% lipoprotein-deficient serum (LPDS, Sigma #S5519) or 1% LPDS control ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were then further incubated with 20 µL of N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide with 1% tert-Butyldimethylchlorosilane (TBDMS) (Sigma) at 60 °C for 60 min ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ul of reaction was passed over a Dowex cation exchange resin column (N-methyl D-glucamine form) (217484; Millipore-Sigma) to remove any uninternalized 14C-lysine ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were treated with 1 ml pre-cooled (-20°C) mixture of methyl tert-butyl ether (MTBE, Sigma 306975), Optima LC-MS grade methanol (A456-212 ...
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bioRxiv - Molecular Biology 2023Quote: 2 x 106 hVSMCs were seeded in to tissue culture flasks and incubated with 15 mL of serum free complete medium (Cat. #311-500) for 24 hours under control or cholesterol treated (methyl-β-cyclodextrin-cholesterol mixture (5µg/ml, Sigma)) conditions using an established protocol35 ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were treated with 1 ml pre-cooled (-20°C) mixture of methyl tert-butyl ether (MTBE, Sigma 306975), Optima LC-MS grade methanol (A456-212 ...