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Citations for Millipore Sigma :
8901 - 8950 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... ALP activity was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Cell Biology 2019Quote: ... Central channels were loaded with cells and 2 mg/mL fibrinogen after addition of bovine thrombin (2 U/ml) (Sigma). After polymerization ...
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bioRxiv - Microbiology 2019Quote: ... referred to here as hCMEC were obtained from Millipore and were maintained in an EndoGRO-MV complete medium kit supplemented with 1 ng/ml fibroblast growth factor-2 (FGF-2; Millipore) (81–84) ...
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bioRxiv - Developmental Biology 2019Quote: ... Dechorionated live embryos were incubated for 6, 12 and 24 hours in 40μM Nifedipine or 20mM BDM (2, 3-butanedione-2-monoxime) (Sigma-Aldrich) in Danieau’s solution ...
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bioRxiv - Immunology 2020Quote: ... the membranes were incubated with the appropriate horseradish-peroxidase conjugated secondary Ab (1:5000 in TBST + 2% BSA; 2 h at room temperature; anti-mouse A4416 and anti-rabbit A0545, Sigma). The immunoreactivity was detected by ECL reagents (Amersham) ...
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bioRxiv - Cell Biology 2021Quote: ... and re-suspended in equal volume of 2×Native lysis buffer (200mM Tris pH 7.5, 150mM NaCl, 2mM PMSF, 2×Protease Inhibitor Cocktails (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2021Quote: ... of 2 in the presence of 2.2 mM ferrihydrite (Schwertmann and Fischer, 1973; Stookey, 1970) and 2 mM ferrozine (Sigma-Aldrich). Where indicated ...
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bioRxiv - Neuroscience 2021Quote: ... NEL-MG were treated with 2 μL of red fluorescent latex beads (2 μm, Sigma-Aldrich, St. Louis, MO, USA), HiLyte™ Fluor 488-labeled amyloid β peptide 25-35 (2 μL) ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting pellets were further extracted by sonication in 3X volumes of SDS lysis buffer (50 mM Tris-HCl pH 7.4, 2% SDS, 1X EDTA-free Complete protease inhibitor cocktail [Roche] and 1X phosphatase inhibitor cocktail 2 and 3 [Sigma]). Samples were centrifuged at 21,000g for 30 min at 25°C to obtain the Triton-insoluble (SDS-soluble ...
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bioRxiv - Cell Biology 2021Quote: ... hPSCs were cultured in RPMI B27-medium (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Mesoderm was induced with RPMI B27- (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Skin was minced into small pieces (about 2-3 mm × 2-3 mm) and digested by incubation with 1 mg/mL collagenase V (Sigma) in PBS for one hour with shaking (250 rpm) ...
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bioRxiv - Neuroscience 2021Quote: The following odors/concentrations were used for comparing molecular properties to receptor responses: 1% 2-methyl-2-pentenal (Sigma 294667), 1% trans-cinnamaldehyde (Sigma C80687) ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Immunology 2022Quote: Equimolar amounts of IgG1-F405L and IgG1-K409R antibodies (1 mg/mL) were mixed with freshly prepared 2-mercaptoethylamine (2-MEA; Sigma) solution (750 mM ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A volume of 40 µL of the mix (corresponding to 8.105 cells) was then plated on 6 cm Petri dishes filled with 2 mL of 2% Phytagel (Sigma-Aldrich), following Dubravcic et al (Dubravcic et al. ...
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Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: ... About 2×106 cells of HEK293T cells were transfected with 2 ug of plasmids encoding S proteins using polyetherimide (PEI; Sigma). After 16 hours of incubation ...
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bioRxiv - Plant Biology 2022Quote: ... 100 mM ammonium bicarbonate [AB], 10 mM Tris[2-carboxyethyl]phosphine hydrochloride, 50 mM 2-chloroacetamide, 0.2% protease inhibitor [Sigma, P8340]), and incubated in the dark at room temperature for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were placed into 2 ml cold EZ lysis buffer in a 2 ml glass dounce tissue grinder (Sigma, D8938) and homogenized by hand 25 times with pestle A followed by 25 times with pestle B while incorporating a 180-degree twist ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with PBS-T containing 2% NGS and antisera against Olig-2 (1:500,Millipore) A2B5 (which stains for OPCs ...
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bioRxiv - Neuroscience 2022Quote: ... mice were injected with the noradrenergic neuron specific neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP4; Sigma, C8417) once at a dose of 50 mg/kg (i.p.) ...
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bioRxiv - Bioengineering 2022Quote: ... 80 μL of the supernatant was mixed with 20 μL of 0.75M 2-amino-2-methyl-1-propanol buffer (A65182, Sigma-Aldrich) and 100 μL 100 mM p-nitrophenylphosphate solution (71768 ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... in PBS for 2 h at room temperature and counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Sigma-Aldrich) to identify cell nuclei.
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bioRxiv - Plant Biology 2024Quote: ... (Catalog No: PCT0809, Himedia), 10 mM of 2-Naphthaleneacetic acid (2-NAA, inactive form of auxin) (Catalog No: N4002, Sigma) and 20 nM of synthetic auxin 2,4-Dichlorophenoxyacetic acid (2,4-D ...
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bioRxiv - Genetics 2024Quote: ... acr-2(gf) double mutants were washed into a 2 mL solution of M9 media with 47 mM ethyl methanesulfonate (Sigma) and incubated at 22°C on a spinning wheel for 4 hours ...
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bioRxiv - Immunology 2024Quote: T cell clones were rested overnight in fresh media without IL-2 and added to wells containing matured DCs at 1:1 ratio in the presence of monensin (2 uM, Sigma) and Brefeldin A (5 ug/ml ...
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bioRxiv - Bioengineering 2023Quote: ... substrates containing 400 nm diameter pores at a density of 2·106 pores·cm-2 were coated with 100 µL of 1 µg/mL human plasma fibronectin (Sigma-Aldrich) in PBS (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... and human epithelial type 2 (HEp-2, CCL-23, ATCC) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... or DMSO 0.1% as vehicle was evaluated using a MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) assay as described in (Sanz et al. ...
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bioRxiv - Neuroscience 2023Quote: ... the dyes were removed by flowing 2.5 mL of cleavage buffer comprising 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; Sigma, 646547) with 15 min incubation in the flow chamber to cleave the dyes linked to the readout probes through disulfide bond ...
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bioRxiv - Neuroscience 2023Quote: The mice received three injections every 2 h (200 ul/injection, intraperitoneally in alternative sites of the body) of 5-Chloro-2’-deoxy-Uridine (CldU, Sigma reference C6891 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown overnight in SC-URA medium with 2% dextrose and were plated in SC-URA plates in the presence of 2% dextrose (Sigma) or 2% galactose (Difco) ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then blocked 1 hr at the room temperature (RT) in PBS with 2% Triton-X100 and 2% normal donkey serum (Sigma), followed by an incubation overnight at 4℃ with primary antibody diluted in the blocking solution ...
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bioRxiv - Microbiology 2023Quote: ... the reaction was stopped by FBS then cell were washed 2 time with PBS-based staining buffer containing 2% fetal bovine serum (FBS) and 0.01% NaN3 (Sigma-Aldrich), blocked Fc IgG receptors by pre-incubated with CD16/32(93 ...
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bioRxiv - Biochemistry 2022Quote: Phosphate buffer saline solution (Ca 2+, Mg 2+ free) was prepared using Phosphate Buffer Saline (PBS) powder (Sigma Aldrich, USA.). 9.6 grams of the powdered reagent was added to 990 milliliters of ultra-purified milli-Q water (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilised by washing in 0.1% Triton-X in PBS (PBS-T) for 15 min and incubated for 2 h in a blocking buffer of 2% bovine serum albumin (Sigma) and 3% normal donkey serum (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... pH 9.5) before being transferred to the staining buffer (2% NBT-BCIP stock solution [Sigma-Aldrich, 11681451001] in Buffer 2). Samples were allowed to be stained for 30 min in the dark and were then dehydrated through a series of increasing methanol concentration (20% ...
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bioRxiv - Immunology 2023Quote: Duodenal biopsies were collected in ice-cold RPMI-1640 and processed into single- cell suspensions by treatment with 2 mM EDTA in 2% (v/v) FBS/PBS followed by digestion with collagenase (Sigma) and cryopreservation as previously described.22 PBMCs were isolated from peripheral blood by density gradient centrifugation followed by cryopreservation ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected with 2 µg of scrambled siRNA (MISSION siRNA Universal Negative Control #2 Sigma-Aldrich SIC002-5x1nmol) or 2 µg of MATR3 siRNA (MISSION esiRNA human MATR3 Sigma-Aldrich EHU113801-50UG ...
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bioRxiv - Biochemistry 2023Quote: ... Standard amino acids and 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid (BCH) were purchased from Sigma-Aldrich (St Louis, MO). JPH203 ((S)-2-amino-3-(4-((5-amino-2-phenylbenzo [d]oxazol-7-yl)methoxy)-3,5-dichlorophenyl ...
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bioRxiv - Cell Biology 2024Quote: ... Retinas were manually dissected with gentle up and down passes in ice-cold Ca+2–Mg+2-free Tyrode’s buffer and treated with papain (P3125, Sigma–Aldrich) 20 min and deoxyribonuclease I (18047-019 ...
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bioRxiv - Cell Biology 2024Quote: ... hPSCs were cultured in RPMI B27-medium (RPMI 1640 GlutaMAX, 2% B27 supplement without insulin, 200 µM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Phenotypical reversal of T4-2 cells was performed by adding 10 µM Tyrphostin AG 1478 (Sigma, cat; # 175178-82-2) to the medium on day zero of 3D culture as previously described (Fiore et al. ...
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bioRxiv - Cell Biology 2024Quote: ... To assess glycolysis-related ECAR an additional injection of 100 mM 2-deoxy-d-glucose (2-DG, from Sigma-Aldrich), an inhibitor of glycolysis ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were sequentially treated with short (2 s) applications (separated by 2 min BS washout) of 100 μM GABA (Sigma), 100 μM glutamate (together with 30 μM NMDA receptor co-agonist glycine ...
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bioRxiv - Immunology 2024Quote: ... NK cells were pretreated with DMSO or 2-DG (0.5 mM, 1 mM, 2 mM, 5 mM; Sigma-Aldrich, USA), BAY 87 (10 μM ...
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bioRxiv - Immunology 2024Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30°C for 18 hours at 200rpm ...
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bioRxiv - Immunology 2024Quote: ... Cultures were washed with 4% FBS in PBS and incubated with blocking solution (2% FBS, 2% bovine serum albumin [BSA, A2153; Sigma] ...