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Citations for Millipore Sigma :
8801 - 8850 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used: Anti-phospho-Histone H2A.X (Ser139) (Millipore, #05-636, 1:1000) and FANCD2 (CST ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation of primary anti-cisplatin DNA adducts antibody (1:1000, Millipore) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... mixtures of 100 μM oxaliplatin and 1000 μM cimetidine (Millipore Sigma, cat # C4522) in 5% DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were stripped and re-probed for β-actin (1:1000, Sigma, #A3854). Intensities of HTR2A were normalized against β-actin in the same lane ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-α tubulin antibody (Sigma#T9026, 1:100 IF, 1:1000 WB), mouse anti-BRCA1 D-9 antibody (Santa Cruz Biotechnology#SC6954 ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies used were: rabbit anti-Tyrosine Hydroxylase (TH) (ab152, Millipore, 1:1000), mouse anti-TH (mab318 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated in diluted antibody (ANTI-FLAG, 1:1000, Sigma Aldrich #F1804) in 1% BSA PBST overnight at 4 °C by rocking ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-β-Tubulin (cat. no. T5201, Sigma-Aldrich; IB, 1:1000 dilution) and mouse anti-HA (cat ...
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bioRxiv - Biochemistry 2023Quote: ... and 10% (w/v) glycerol) with proteinase inhibitor cocktail (1:1000, Milipore Sigma). Lysates were centrifuged at 20,000 x g for 10 min at 4 °C and protein concentrations were determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Western blot was performed with anti-FLAG antibody 1:1000 (Sigma Aldrich, #F3165) incubated overnight at 4 °C in PBS-T buffer (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... transferred to PVDF membranes and blotted with anti-FLAG (Sigma, F3165, 1:1000) or anti-His (Cell Signaling #2366 His-Tag 27E8 ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used were mouse anti-FLAG antibody (1:1000, Sigma-Aldrich, F3165), mouse anti-β-actin antibody (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... diluted 1:1000 in TBS-T with 5% Bovine Serum Albumin (BSA, Sigma). Membranes were then washed three times in TBS-T and incubated for 1 h at RT with goat anti-rabbit or goat anti-mouse HRP conjugated secondary antibodies (31460 and 31430 ...
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bioRxiv - Cell Biology 2024Quote: We used rabbit anti-Myosin IIA (Sigma-Aldrich, M8064, 1:1000 for IF), mouse anti-Vinculin (Sigma ...
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bioRxiv - Immunology 2024Quote: ... followed by detection with a goat anti-mouse IgG HRP (1/1000; Millipore) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were counterstained with DAPI (1/1000) and mounted using FluorSave reagent (Millipore).
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bioRxiv - Physiology 2024Quote: ... B-subunit (CTB, 1/1000, goat polyclonal, Calbiochem #227040, Millipore-Merck, Guyancourt, France). The secondary antibody anti-goat was linked to the fluorochromes Alexa Fluor 594 (1/2000 ...
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight at 4 C with primary antibody (DM1a, 1:1000, Sigma T6199), washed 3x 5 min in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Par3 (IF: 1:200, WB: 1:1000, 07-330, EMD Millipore, Burlington, MA), and beta-actin (IF ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-INCENP (Millipore, 05-940, 1:250-IF 1:1000-WB) and Rat anti-Tubulin (EMD Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used: Mouse anti-α-tubulin (1:1000 Sigma T6074); Rabbit anti-α-tubulin (1:2000 Invitrogen PA519489) ...
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bioRxiv - Molecular Biology 2024Quote: ... at a dilution of 1:1000 and Hoechst 33358 (Sigma, cat no# 94403) diluted in blocking buffer for 1hr at room temperature ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:1000 in blocking buffer (PBS 1X containing 1% BSA (Sigma Aldrich) and 0.1% Tween 20) ...
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bioRxiv - Microbiology 2024Quote: ... and mouse monoclonal anti-HCoV-OC43 nucleocapsid protein (1:1000, Millipore Sigma, MAB9012). Secondary antibodies coupled to HRP were purchased from molecular probes and Jackson Immuno-research.
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bioRxiv - Molecular Biology 2024Quote: ... DAPI was used as a counterstain for nuclei (1:1000, Sigma-Aldrich D9542).
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bioRxiv - Biochemistry 2024Quote: Following antibodies were used: anti-FLAG® M2 (1:1000, Sigma Aldrich, F3165), anti-FLAG (1:500 ...
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bioRxiv - Biophysics 2021Quote: ... 2 μL of 100 mM 5,5′-dithiobis-(2-nitrobenzoic acid) (Sigma Aldrich, dissolved in 50 mM NaH2PO4, pH 7.5) was immediately added and fully mixed by pipetting before incubating at room temperature for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... These were digested with 2 mg/ml collagenase IV and 2 mg/ml hyaluronidase (Sigma-Aldrich, St Louis, USA) at 37 °C for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... 150 mM NaCl, 2 mM EGTA, 2 mM MgCl2, 0.5% NP-40, 1mM DTT, 1U/ml Benzonase from Novagen/EMD Millipore ...
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bioRxiv - Immunology 2021Quote: ... BMDMs were pretreated for 30 min with hexokinase inhibitor 2-deoxy-D-glucose (2-DG; 10 mM, Sigma-Aldrich), actin polymerization inhibitor cytochalasin D (10 μM ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... Peptides were separated using a 250 min gradient ranging from 2 % to 90 % mobile phase B (mobile phase A: 2% acetonitrile [Scharlab S.L.], 0.1 % formic acid [Sigma-Aldrich] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2-Methoxy-3-methyl-1,4-BQ was synthesized as follows: 2 mmol 2-Methoxy-3-methyl-1,4-hydroquinone (Sigma Aldrich) and 2 mmol NaIO4 were mixed in a 50 ml round-bottom flask and 10 ml CH2Cl2 and 5 ml of water were then added ...
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bioRxiv - Cancer Biology 2019Quote: ... Reagents used including 5-chloro-2′-deoxyuridine (CldU) and 5-Iodo-2′-deoxyuridine (IdU) were obtained from Sigma-Aldrich. Concentration and duration of treatment are indicated in the corresponding figures and sections.
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bioRxiv - Immunology 2019Quote: Cell viability was monitored by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT; Sigma, USA) assay as previously described [52] ...
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bioRxiv - Molecular Biology 2019Quote: ... the collagenase inhibitor Ilomastat ((R)-N′-Hydroxy-N-[(S)-2-indol-3-yl-1-(methylcarbamoyl)ethyl]-2-isobutylsuccinamide) (25μM, CC1010, Merck Millipore) or a combination of both ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were incubated with 2 μg/mL 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, Missouri, USA) for 10 minutes as the final step in all flow cytometry assays to distinguish membrane integrity and disruption ...
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bioRxiv - Microbiology 2021Quote: ... pellets were resuspended in 2 ml 10 mM Tris HCl (pH 7.4) containing 2 mg ml−1 pronase (Sigma). Tubes were incubated for 3 h at 60 °C to digest proteins ...
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bioRxiv - Bioengineering 2020Quote: ... yeast extract 5 g/L, 2.7 g/L Na2HPO4, 1.5 g/L citric acid, 2% glucose, 2% cellulase from Trichoderma reesei [Sigma-Aldrich]) in shaking conditions at 250 rpm/min and at 30°C for 3 days ...
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bioRxiv - Cancer Biology 2020Quote: Both pBabe-SAOS 2 and hTERT-SAOS 2 (107 cells) were cross-linked with 1% paraformaldehyde (Sigma Aldrich, # P6148) in culture medium for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Triton-X buffer was supplemented further with 2 μM olaparib (Selleckchem) and 2 μM PARG inhibitor PDD00017273 (Sigma Aldrich)
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bioRxiv - Cell Biology 2021Quote: β-cell like organoids (day 31) were fixed in a freshly prepared mixture of 2 % formaldehyde and 2 % glutaraldehyde (Sigma) in 0.1 M phosphate buffer (18.2% 0.1 M KH2PO4 ...
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bioRxiv - Neuroscience 2019Quote: ... They were then transferred to carbogenated ACSF without sucrose (NaCl, 125; KCL, 3.5; NaH2PO4, 1.25, NaHCO3, 26; CaCl2, 2; MgCl2, 2; glucose, 20; from Sigma-Aldrich) and were used for experiments after at least one hour ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated at room temperature in carbogenated aCSF (125 NaCl, 25 NaHCO3, 2.5 KCl, 1.25 NaH2PO4, 2 CaCl2, 2 MgCl2 and 25 glucose, all from Sigma Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... cells growing in either 6-well plates or 10-cm dish in log phase were rinsed with PBS twice and lysed on ice in 100-500 ul 2x Sample buffer (80 mM Tris pH6.8, 2% SDS, 10% Glycerol, 0.0006% Bromophenol blue) containing 280 mM 2-Mercaptoethanol (Sigma #M7522), 1x Aprotinin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 126 NaCl, 2.5 KCl, 1.25 NaH2PO4, 2 MgCl2, 2 CaCl2, 26 NaHCO3, and 10 glucose, 311 mOsm, Sigma-Aldrich) at 34°C for at least one hour before recording ...
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bioRxiv - Biochemistry 2020Quote: ... The peptide concentration was 20 μM in 100 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) (Sigma Aldrich) buffer (pH 7.4) ...
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bioRxiv - Biophysics 2019Quote: ... 200 μl of RPMI medium containing a distinct concentration of PMA (0.2, 2, 20, 200 nM) or lipopolysaccharide from Pseudomonas Aeruginosa (LPS) (0.2, 2, 20, 200 μg/ml) (Sigma-Aldrich) were added to a well in a blinded fashion and phase contrast as well as DAPI channels were recorded every two minutes for 160 minutes to image the cell movement.
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bioRxiv - Immunology 2021Quote: Murine NK cells were incubated for 2 h in the presence of GABA (2 or 10 μM, Sigma-Aldrich), supernatants from unchallenged mBMDCs or supernatants from mBMDCs challenged with T ...
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bioRxiv - Microbiology 2019Quote: ... MRS was supplemented with 2% glucose (glc) (MRS-G) or 2% fructose (frc) (MRS-F) (Sigma-Aldrich, Espoo Finland) or less (1.75% ...
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bioRxiv - Biophysics 2021Quote: ... extraction solution #1 was replaced by extraction solution #2 (2% Triton X-100, 1% CHAPS hydrate; Sigma Aldrich, Germany) for 5 min ...